In pursuit of a minimal CHO genome: Establishment of large-scale genome deletions
N Biotechnol. 2023 Dec 26:S1871-6784(23)00075-4. doi: 10.1016/j.nbt.2023.12.007. Online ahead of print.ABSTRACTChinese hamster ovary (CHO) cells are the most commonly used mammalian cell line for the production of complex therapeutic glycoproteins. As CHO cells have evolved as part of a multicellular organism, they harbor many cellular functions irrelevant for their application as production hosts in industrial bioprocesses. Consequently, CHO cells have been the target for numerous genetic engineering efforts in the past, but a tailored host cell chassis holistically optimized for its specific task in a bioreactor is still...
Source: New Biotechnology - December 28, 2023 Category: Biotechnology Authors: Tobias Jerabek Linus Wei ß Hannah Fahrion Nikolas Zeh Nadja Raab Benjamin Lindner Simon Fischer Kerstin Otte Source Type: research
Inefficient transcription is a production bottleneck for artificial therapeutic BiTE ® proteins
N Biotechnol. 2023 Dec 26:S1871-6784(23)00076-6. doi: 10.1016/j.nbt.2023.12.008. Online ahead of print.ABSTRACTAntibodies are potent biopharmaceuticals used to treat severe diseases, including cancers. During the past decade, more complex modalities have been developed including bispecific T-cell engager (BiTE®) molecules, e.g. by Amgen. However, non-natural and complex molecule formats often prove to be difficult-to-express (DTE), which is the case for BiTE® molecules. Due to the growing importance of multispecific modalities such as half-life extended (HLE) BiTE® and HLE dual-targeting bispecific T-cell engager (dBiTE...
Source: New Biotechnology - December 28, 2023 Category: Biotechnology Authors: Tobias Jerabek Madina Burkhart Selina Goetz Benedikt Greck Anika Menthe Ruediger Neef Kerstin Otte Source Type: research
Pre-analytical properties of different respiratory viruses for PCR-based detection: Comparative analysis of sampling devices and sample stabilization solutions
N Biotechnol. 2023 Dec 23:S1871-6784(23)00073-0. doi: 10.1016/j.nbt.2023.12.005. Online ahead of print.ABSTRACTAfter the decline of the COVID-19 pandemic, health systems were challenged by the simultaneous prevalence of different respiratory viruses causing a wide overlap in symptoms. This increased the demand for multi-virus diagnostic tests which require suitable pre-analytical workflow solutions in order to receive valid diagnostic results. In this context, the effects of specimen storage duration and temperature on the RNA/DNA copy number stability of influenza A/B, RSV A/B, SARS-CoV-2 and adenovirus were examined for ...
Source: New Biotechnology - December 25, 2023 Category: Biotechnology Authors: Melina Hardt Franziska Kaiser Thorsten Voss Uwe Oelm üller Kurt Zatloukal Source Type: research
Pre-analytical properties of different respiratory viruses for PCR-based detection: Comparative analysis of sampling devices and sample stabilization solutions
N Biotechnol. 2023 Dec 23:S1871-6784(23)00073-0. doi: 10.1016/j.nbt.2023.12.005. Online ahead of print.ABSTRACTAfter the decline of the COVID-19 pandemic, health systems were challenged by the simultaneous prevalence of different respiratory viruses causing a wide overlap in symptoms. This increased the demand for multi-virus diagnostic tests which require suitable pre-analytical workflow solutions in order to receive valid diagnostic results. In this context, the effects of specimen storage duration and temperature on the RNA/DNA copy number stability of influenza A/B, RSV A/B, SARS-CoV-2 and adenovirus were examined for ...
Source: New Biotechnology - December 25, 2023 Category: Biotechnology Authors: Melina Hardt Franziska Kaiser Thorsten Voss Uwe Oelm üller Kurt Zatloukal Source Type: research
Pre-analytical properties of different respiratory viruses for PCR-based detection: Comparative analysis of sampling devices and sample stabilization solutions
N Biotechnol. 2023 Dec 23:S1871-6784(23)00073-0. doi: 10.1016/j.nbt.2023.12.005. Online ahead of print.ABSTRACTAfter the decline of the COVID-19 pandemic, health systems were challenged by the simultaneous prevalence of different respiratory viruses causing a wide overlap in symptoms. This increased the demand for multi-virus diagnostic tests which require suitable pre-analytical workflow solutions in order to receive valid diagnostic results. In this context, the effects of specimen storage duration and temperature on the RNA/DNA copy number stability of influenza A/B, RSV A/B, SARS-CoV-2 and adenovirus were examined for ...
Source: New Biotechnology - December 25, 2023 Category: Biotechnology Authors: Melina Hardt Franziska Kaiser Thorsten Voss Uwe Oelm üller Kurt Zatloukal Source Type: research
Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater
N Biotechnol. 2023 Dec 19;79:50-59. doi: 10.1016/j.nbt.2023.12.003. Online ahead of print.ABSTRACTBetter understanding of macrophyte tolerance under long exposure times in real environmental matrices is crucial for phytoremediation and phytoattenuation strategies for aquatic systems. The metal(loid) attenuation ability of 10 emergent macrophyte species (Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus, and Typha angustifolia) was investigated using real groundwater from an industrial site, over a 90-day exposure ...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Blanca Velasco-Arroyo Sandra Curiel-Alegre Aqib Hassan Ali Khan Carlos Rumbo Daniel P érez-Alonso Carlos Rad Herwig De Wilde Alfredo P érez-de-Mora Roc ío Barros Source Type: research
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein
N Biotechnol. 2023 Dec 19:S1871-6784(23)00072-9. doi: 10.1016/j.nbt.2023.12.004. Online ahead of print.ABSTRACT'Epivolve' (epitope evolution) is an innovative paratope-evolving technology using a haptenated peptide or protein immunogen as a means of directing the in vivo immune response to specifically targeted sites at a one amino acid residue resolution. Guided by protein structural analysis, Epivolve technology was tested to develop site-directed neutralizing antibodies (nAbs) in a systematic fashion against the SARS-CoV-2 Receptor Binding Domain (RBD). Thirteen solvent-exposed sites covering the ACE2 receptor-binding i...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Xiaofeng Li Liudmila Kulakova Eric A Toth Kezzia Jones Thomas R Fuerst Michael P Weiner Source Type: research
Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater
N Biotechnol. 2023 Dec 19:S1871-6784(23)00071-7. doi: 10.1016/j.nbt.2023.12.003. Online ahead of print.ABSTRACTBetter understanding of macrophyte tolerance under long exposure times in real environmental matrices is crucial for phytoremediation and phytoattenuation strategies for aquatic systems. The metal(loid) attenuation ability of 10 emergent macrophyte species (Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus, and Typha angustifolia) was investigated using real groundwater from an industrial site, over a 90-...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Blanca Velasco-Arroyo Sandra Curiel-Alegre Aqib Hassan Ali Khan Carlos Rumbo Perez-AlonsoDaniel P érez-Alonso Carlos Rad Herwig De Wilde Alfredo P érez-de-Mora Roc ío Barros Source Type: research
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein
N Biotechnol. 2023 Dec 19:S1871-6784(23)00072-9. doi: 10.1016/j.nbt.2023.12.004. Online ahead of print.ABSTRACT'Epivolve' (epitope evolution) is an innovative paratope-evolving technology using a haptenated peptide or protein immunogen as a means of directing the in vivo immune response to specifically targeted sites at a one amino acid residue resolution. Guided by protein structural analysis, Epivolve technology was tested to develop site-directed neutralizing antibodies (nAbs) in a systematic fashion against the SARS-CoV-2 Receptor Binding Domain (RBD). Thirteen solvent-exposed sites covering the ACE2 receptor-binding i...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Xiaofeng Li Liudmila Kulakova Eric A Toth Kezzia Jones Thomas R Fuerst Michael P Weiner Source Type: research
Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater
N Biotechnol. 2023 Dec 19:S1871-6784(23)00071-7. doi: 10.1016/j.nbt.2023.12.003. Online ahead of print.ABSTRACTBetter understanding of macrophyte tolerance under long exposure times in real environmental matrices is crucial for phytoremediation and phytoattenuation strategies for aquatic systems. The metal(loid) attenuation ability of 10 emergent macrophyte species (Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus, and Typha angustifolia) was investigated using real groundwater from an industrial site, over a 90-...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Blanca Velasco-Arroyo Sandra Curiel-Alegre Aqib Hassan Ali Khan Carlos Rumbo Perez-AlonsoDaniel P érez-Alonso Carlos Rad Herwig De Wilde Alfredo P érez-de-Mora Roc ío Barros Source Type: research
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein
N Biotechnol. 2023 Dec 19:S1871-6784(23)00072-9. doi: 10.1016/j.nbt.2023.12.004. Online ahead of print.ABSTRACT'Epivolve' (epitope evolution) is an innovative paratope-evolving technology using a haptenated peptide or protein immunogen as a means of directing the in vivo immune response to specifically targeted sites at a one amino acid residue resolution. Guided by protein structural analysis, Epivolve technology was tested to develop site-directed neutralizing antibodies (nAbs) in a systematic fashion against the SARS-CoV-2 Receptor Binding Domain (RBD). Thirteen solvent-exposed sites covering the ACE2 receptor-binding i...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Xiaofeng Li Liudmila Kulakova Eric A Toth Kezzia Jones Thomas R Fuerst Michael P Weiner Source Type: research
Phytostabilization of metal(loid)s by ten emergent macrophytes following a 90-day exposure to industrially contaminated groundwater
N Biotechnol. 2023 Dec 19:S1871-6784(23)00071-7. doi: 10.1016/j.nbt.2023.12.003. Online ahead of print.ABSTRACTBetter understanding of macrophyte tolerance under long exposure times in real environmental matrices is crucial for phytoremediation and phytoattenuation strategies for aquatic systems. The metal(loid) attenuation ability of 10 emergent macrophyte species (Carex riparia, Cyperus longus, Cyperus rotundus, Iris pseudacorus, Juncus effusus, Lythrum salicaria, Menta aquatica, Phragmites australis, Scirpus holoschoenus, and Typha angustifolia) was investigated using real groundwater from an industrial site, over a 90-...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Blanca Velasco-Arroyo Sandra Curiel-Alegre Aqib Hassan Ali Khan Carlos Rumbo Perez-AlonsoDaniel P érez-Alonso Carlos Rad Herwig De Wilde Alfredo P érez-de-Mora Roc ío Barros Source Type: research
Site-directed neutralizing antibodies targeting structural sites on SARS-CoV-2 spike protein
N Biotechnol. 2023 Dec 19:S1871-6784(23)00072-9. doi: 10.1016/j.nbt.2023.12.004. Online ahead of print.ABSTRACT'Epivolve' (epitope evolution) is an innovative paratope-evolving technology using a haptenated peptide or protein immunogen as a means of directing the in vivo immune response to specifically targeted sites at a one amino acid residue resolution. Guided by protein structural analysis, Epivolve technology was tested to develop site-directed neutralizing antibodies (nAbs) in a systematic fashion against the SARS-CoV-2 Receptor Binding Domain (RBD). Thirteen solvent-exposed sites covering the ACE2 receptor-binding i...
Source: New Biotechnology - December 21, 2023 Category: Biotechnology Authors: Xiaofeng Li Liudmila Kulakova Eric A Toth Kezzia Jones Thomas R Fuerst Michael P Weiner Source Type: research
Bioinformatics and functional selection of GH77 4- α-glucanotransferases for potato starch modification
N Biotechnol. 2023 Dec 12;79:39-49. doi: 10.1016/j.nbt.2023.12.002. Online ahead of print.ABSTRACT4-α-glucanotransferases (4αGTs, EC 2.4.1.25) from glycoside hydrolase family 77 (GH77) catalyze chain elongation of starch amylopectin chains and can be utilized to structurally modify starch to tailor its gelation properties. The potential relationship between the structural design of 4αGTs and functional starch modification is unknown. Here, family GH77 was mined in silico for enzyme candidates based on sub-grouping guided by Conserved Unique Peptide Patterns (CUPP) bioinformatics categorization. From + 12,000 protein seq...
Source: New Biotechnology - December 14, 2023 Category: Biotechnology Authors: Stefan Jarl Christensen Michael Schmidt Madsen Signe Schram Zinck Christinne Hedberg Ole Bandsholm S ørensen Birte Svensson Anne S Meyer Source Type: research
Bioinformatics and functional selection of GH77 4- α-glucanotransferases for potato starch modification
N Biotechnol. 2023 Dec 12:S1871-6784(23)00070-5. doi: 10.1016/j.nbt.2023.12.002. Online ahead of print.ABSTRACT4-α-glucanotransferases (4αGTs, EC 2.4.1.25) from glycoside hydrolase family 77 (GH77) catalyze chain elongation of starch amylopectin chains and can be utilized to structurally modify starch to tailor its gelation properties. The potential relationship between the structural design of 4αGTs and functional starch modification is unknown. Here, family GH77 was mined in silico for enzyme candidates based on sub-grouping guided by Conserved Unique Peptide Patterns (CUPP) bioinformatics categorization. From +12,000...
Source: New Biotechnology - December 14, 2023 Category: Biotechnology Authors: Stefan Jarl Christensen Michael Schmidt Madsen Signe Schram Zinck Christinne Hedberg Ole Bandsholm S ørensen Birte Svensson Anne S Meyer Source Type: research
