Earth: Extinguishing anthropogenic risks through harmonization
N Biotechnol. 2024 Feb 15;80:69-71. doi: 10.1016/j.nbt.2024.02.002. Online ahead of print.ABSTRACTHuman diseases can kill one person at a time, but the COVID-19 pandemic showed massacres could be possible. The climate crisis could be even worse, potentially leading to a bigger number of deaths of the human species and all living systems on Earth. I urge us to change our human-focused mindset to solve many problems, including the climate crisis, which humans caused to the entire ecosystems due to our arrogance: humans own this world. In this perspective article, I propose four recommendations to address climate issues throu...
Source: New Biotechnology - February 17, 2024 Category: Biotechnology Authors: Tae Seok Moon Source Type: research
Earth: Extinguishing Anthropogenic Risks Through Harmonization
N Biotechnol. 2024 Feb 15:S1871-6784(24)00004-9. doi: 10.1016/j.nbt.2024.02.002. Online ahead of print.ABSTRACTHuman diseases can kill one person at a time, but the COVID-19 pandemic showed massacres could be possible. The climate crisis could be even worse, potentially leading to a bigger number of deaths of the human species and all living systems on Earth. I urge us to change our human-focused mindset to solve many problems, including the climate crisis, which humans caused to the entire ecosystems due to our arrogance: humans own this world. In this perspective article, I propose four recommendations to address climate...
Source: New Biotechnology - February 17, 2024 Category: Biotechnology Authors: Tae Seok Moon Source Type: research
Earth: Extinguishing Anthropogenic Risks Through Harmonization
N Biotechnol. 2024 Feb 15:S1871-6784(24)00004-9. doi: 10.1016/j.nbt.2024.02.002. Online ahead of print.ABSTRACTHuman diseases can kill one person at a time, but the COVID-19 pandemic showed massacres could be possible. The climate crisis could be even worse, potentially leading to a bigger number of deaths of the human species and all living systems on Earth. I urge us to change our human-focused mindset to solve many problems, including the climate crisis, which humans caused to the entire ecosystems due to our arrogance: humans own this world. In this perspective article, I propose four recommendations to address climate...
Source: New Biotechnology - February 17, 2024 Category: Biotechnology Authors: Tae Seok Moon Source Type: research
Earth: Extinguishing Anthropogenic Risks Through Harmonization
N Biotechnol. 2024 Feb 15:S1871-6784(24)00004-9. doi: 10.1016/j.nbt.2024.02.002. Online ahead of print.ABSTRACTHuman diseases can kill one person at a time, but the COVID-19 pandemic showed massacres could be possible. The climate crisis could be even worse, potentially leading to a bigger number of deaths of the human species and all living systems on Earth. I urge us to change our human-focused mindset to solve many problems, including the climate crisis, which humans caused to the entire ecosystems due to our arrogance: humans own this world. In this perspective article, I propose four recommendations to address climate...
Source: New Biotechnology - February 17, 2024 Category: Biotechnology Authors: Tae Seok Moon Source Type: research
Deep mining of antibody phage-display selections using Oxford Nanopore Technologies and Dual Unique Molecular Identifiers
N Biotechnol. 2024 Feb 12:S1871-6784(24)00003-7. doi: 10.1016/j.nbt.2024.02.001. Online ahead of print.ABSTRACTAntibody phage-display technology identifies antibody-antigen interactions through multiple panning rounds, but traditional screening gives no information on enrichment or diversity throughout the process. This results in the loss of valuable binders. Next Generation Sequencing can overcome this problem. We introduce a high accuracy long-read sequencing method based on the recent Oxford Nanopore Technologies (ONT) Q20+ chemistry in combination with dual unique molecular identifiers (UMIs) and an optimized bioinfor...
Source: New Biotechnology - February 14, 2024 Category: Biotechnology Authors: Oscar Mejias-Gomez Marta Braghetto Morten Kielsgaard Dziegiel S ørensen Andreas Visbech Madsen Laura Salse Guiu Peter Kristensen Lasse Ebdrup Pedersen Steffen Goletz Source Type: research
Deep mining of antibody phage-display selections using Oxford Nanopore Technologies and Dual Unique Molecular Identifiers
N Biotechnol. 2024 Feb 12:S1871-6784(24)00003-7. doi: 10.1016/j.nbt.2024.02.001. Online ahead of print.ABSTRACTAntibody phage-display technology identifies antibody-antigen interactions through multiple panning rounds, but traditional screening gives no information on enrichment or diversity throughout the process. This results in the loss of valuable binders. Next Generation Sequencing can overcome this problem. We introduce a high accuracy long-read sequencing method based on the recent Oxford Nanopore Technologies (ONT) Q20+ chemistry in combination with dual unique molecular identifiers (UMIs) and an optimized bioinfor...
Source: New Biotechnology - February 14, 2024 Category: Biotechnology Authors: Oscar Mejias-Gomez Marta Braghetto Morten Kielsgaard Dziegiel S ørensen Andreas Visbech Madsen Laura Salse Guiu Peter Kristensen Lasse Ebdrup Pedersen Steffen Goletz Source Type: research
Deep mining of antibody phage-display selections using Oxford Nanopore Technologies and Dual Unique Molecular Identifiers
N Biotechnol. 2024 Feb 12:S1871-6784(24)00003-7. doi: 10.1016/j.nbt.2024.02.001. Online ahead of print.ABSTRACTAntibody phage-display technology identifies antibody-antigen interactions through multiple panning rounds, but traditional screening gives no information on enrichment or diversity throughout the process. This results in the loss of valuable binders. Next Generation Sequencing can overcome this problem. We introduce a high accuracy long-read sequencing method based on the recent Oxford Nanopore Technologies (ONT) Q20+ chemistry in combination with dual unique molecular identifiers (UMIs) and an optimized bioinfor...
Source: New Biotechnology - February 14, 2024 Category: Biotechnology Authors: Oscar Mejias-Gomez Marta Braghetto Morten Kielsgaard Dziegiel S ørensen Andreas Visbech Madsen Laura Salse Guiu Peter Kristensen Lasse Ebdrup Pedersen Steffen Goletz Source Type: research
Robust and resource-efficient production process suitable for large-scale production of baculovirus through high cell density seed train and optimized infection strategy
N Biotechnol. 2024 Feb 1;80:46-55. doi: 10.1016/j.nbt.2024.01.002. Online ahead of print.ABSTRACTThe aim of this study was the development of a scalable production process for high titer (108 pfu/mL and above) recombinant baculovirus stocks with low cell line-derived impurities for the production of virus-like particles (VLP). To achieve this, we developed a high cell density (HCD) culture for low footprint cell proliferation, compared different infection strategies at multiplicity of infection (MOI) 0.05 and 0.005, different infection strategies and validated generally applicable harvest criteria of cell viability ≤ 80%...
Source: New Biotechnology - February 1, 2024 Category: Biotechnology Authors: Lena Achleitner Martina Winter Patricia Pereira Aguilar Nico Lingg Alois Jungbauer Miriam Klausberger Peter Satzer Source Type: research
From food waste to eco-friendly functionalized polymer composites: Investigation of orange peels as active filler
N Biotechnol. 2024 Jan 20;80:37-45. doi: 10.1016/j.nbt.2024.01.001. Online ahead of print.ABSTRACTThe development of eco-friendly polymer composites with multifunctional properties aligns with the goals of the circular economy agenda, which aims to minimize waste and promote the sustainable use of resources by closing the loop of product life cycles. Eco-friendly polymer composites play a crucial role in achieving these objectives. The present work focuses on the preparation of fully biobased blends obtained by melt mixing a bio-polyester, poly(butylene succinate-co-adipate) (PBSA), with orange peels up to 20 wt%, to yield...
Source: New Biotechnology - January 22, 2024 Category: Biotechnology Authors: Elia Pagliarini Carmen Minichiello Laura Sisti Grazia Totaro Loredana Baffoni Diana Di Gioia Andrea Saccani Source Type: research
Protein coding regions prediction by fusing DNA shape features
N Biotechnol. 2024 Jan 3;80:21-26. doi: 10.1016/j.nbt.2023.12.006. Online ahead of print.ABSTRACTExons crucial for coding are often hidden within introns, and the two tend to vary greatly in length, which results in deep learning-based protein coding region prediction methods often performing poorly when applied to more structurally complex biological genomes. DNA shape information also plays a role in revealing the underlying logic of gene expression, yet current methods ignore the influence of DNA shape features when distinguishing coding and non-coding regions. We propose a method to predict protein-coding regions using...
Source: New Biotechnology - January 5, 2024 Category: Biotechnology Authors: Miao Chen Yangyang Li Kun Zhang Hao Liu Source Type: research
Protein coding regions prediction by fusing DNA shape features
N Biotechnol. 2024 Jan 3:S1871-6784(23)00074-2. doi: 10.1016/j.nbt.2023.12.006. Online ahead of print.ABSTRACTExons crucial for coding are often hidden within introns, and the two tend to vary greatly in length, which results in deep learning-based protein coding region prediction methods often performing poorly when applied to more structurally complex biological genomes. DNA shape information also plays a role in revealing the underlying logic of gene expression, yet current methods ignore the influence of DNA shape features when distinguishing coding and non-coding regions. We propose a method to predict protein-coding ...
Source: New Biotechnology - January 5, 2024 Category: Biotechnology Authors: Miao Chen Yangyang Li Kun Zhang Hao Liu Source Type: research
Protein coding regions prediction by fusing DNA shape features
N Biotechnol. 2024 Jan 3:S1871-6784(23)00074-2. doi: 10.1016/j.nbt.2023.12.006. Online ahead of print.ABSTRACTExons crucial for coding are often hidden within introns, and the two tend to vary greatly in length, which results in deep learning-based protein coding region prediction methods often performing poorly when applied to more structurally complex biological genomes. DNA shape information also plays a role in revealing the underlying logic of gene expression, yet current methods ignore the influence of DNA shape features when distinguishing coding and non-coding regions. We propose a method to predict protein-coding ...
Source: New Biotechnology - January 5, 2024 Category: Biotechnology Authors: Miao Chen Yangyang Li Kun Zhang Hao Liu Source Type: research
Protein coding regions prediction by fusing DNA shape features
N Biotechnol. 2024 Jan 3:S1871-6784(23)00074-2. doi: 10.1016/j.nbt.2023.12.006. Online ahead of print.ABSTRACTExons crucial for coding are often hidden within introns, and the two tend to vary greatly in length, which results in deep learning-based protein coding region prediction methods often performing poorly when applied to more structurally complex biological genomes. DNA shape information also plays a role in revealing the underlying logic of gene expression, yet current methods ignore the influence of DNA shape features when distinguishing coding and non-coding regions. We propose a method to predict protein-coding ...
Source: New Biotechnology - January 5, 2024 Category: Biotechnology Authors: Miao Chen Yangyang Li Kun Zhang Hao Liu Source Type: research
Live cell pool and rare cell isolation using Enrich TROVO system
N Biotechnol. 2024 Jan 2;80:12-20. doi: 10.1016/j.nbt.2023.12.013. Online ahead of print.ABSTRACTAlthough several technologies have been developed to isolate cells of interest from a heterogenous sample, clogging and impaired cell viability limit such isolation. We have developed the Enrich TROVO system as a novel, nonfluidic technology to sort live cells. The TROVO system combines imaging-based cell selection and photo-crosslinking of (gelatin methacrylate) gelMA-hydrogel to capture cells. After capture, cells are released by enzymatic digestion of the hydrogel and then retrieved for downstream analysis or further cell cu...
Source: New Biotechnology - January 4, 2024 Category: Biotechnology Authors: Stephen Rotatori Yichong Zhang Kirby Madden-Hennessey Christina Mohammed Chi-Han Yang Jordan Urbani Prem Shrestha Joseph Pettinelli Dong Wang Xueqi Liu Qi Zhao Source Type: research
Live cell pool and rare cell isolation using Enrich TROVO system
N Biotechnol. 2024 Jan 2:S1871-6784(23)00081-X. doi: 10.1016/j.nbt.2023.12.013. Online ahead of print.ABSTRACTAlthough several technologies have been developed to isolate cells of interest from a heterogenous sample, clogging and impaired cell viability limit such isolation. We have developed the Enrich TROVO system as a novel, nonfluidic technology to sort live cells. The TROVO system combines imaging-based cell selection and photo-crosslinking of (gelatin methacrylate) gelMA-hydrogel to capture cells. After capture, cells are released by enzymatic digestion of the hydrogel and then retrieved for downstream analysis or fu...
Source: New Biotechnology - January 4, 2024 Category: Biotechnology Authors: Stephen Rotatori Yichong Zhang Kirby Madden-Hennessey Christina Mohammed Chi-Han Yang Jordan Urbani Prem Shrestha Joseph Pettinelli Dong Wang Xueqi Liu Qi Zhao Source Type: research
