Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: the periodic hydrodynamic loading and the shear stress accumulation
N Biotechnol. 2023 Jul 11:S1871-6784(23)00036-5. doi: 10.1016/j.nbt.2023.07.002. Online ahead of print.ABSTRACTIn vitro production of blood platelets for transfusion purposes is gaining interest. While platelet production is now possible on a laboratory scale, the challenge is to move towards industrial production. Attaining this goal calls for the development of platelet release devices capable of producing large quantities of platelets. To this end, we have developed a continuous-flow platelet release device composed of five spherical chambers each containing two calibrated cones placed in a staggered configuration. Foll...
Source: New Biotechnology - July 13, 2023 Category: Biotechnology Authors: Ana ïs Pongérard L éa Mallo Valentin Do Sacramento Olivier Boiron Anita Eckly Christian Gachet Fran çois Lanza Yannick Knapp Catherine Strassel Source Type: research
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: the periodic hydrodynamic loading and the shear stress accumulation
N Biotechnol. 2023 Jul 11:S1871-6784(23)00036-5. doi: 10.1016/j.nbt.2023.07.002. Online ahead of print.ABSTRACTIn vitro production of blood platelets for transfusion purposes is gaining interest. While platelet production is now possible on a laboratory scale, the challenge is to move towards industrial production. Attaining this goal calls for the development of platelet release devices capable of producing large quantities of platelets. To this end, we have developed a continuous-flow platelet release device composed of five spherical chambers each containing two calibrated cones placed in a staggered configuration. Foll...
Source: New Biotechnology - July 13, 2023 Category: Biotechnology Authors: Ana ïs Pongérard L éa Mallo Valentin Do Sacramento Olivier Boiron Anita Eckly Christian Gachet Fran çois Lanza Yannick Knapp Catherine Strassel Source Type: research
Development of an efficient, ready to use, blood platelet-release device based on two new flow regime parameters: the periodic hydrodynamic loading and the shear stress accumulation
N Biotechnol. 2023 Jul 11:S1871-6784(23)00036-5. doi: 10.1016/j.nbt.2023.07.002. Online ahead of print.ABSTRACTIn vitro production of blood platelets for transfusion purposes is gaining interest. While platelet production is now possible on a laboratory scale, the challenge is to move towards industrial production. Attaining this goal calls for the development of platelet release devices capable of producing large quantities of platelets. To this end, we have developed a continuous-flow platelet release device composed of five spherical chambers each containing two calibrated cones placed in a staggered configuration. Foll...
Source: New Biotechnology - July 13, 2023 Category: Biotechnology Authors: Ana ïs Pongérard L éa Mallo Valentin Do Sacramento Olivier Boiron Anita Eckly Christian Gachet Fran çois Lanza Yannick Knapp Catherine Strassel Source Type: research
Taming CRISPRi: Dynamic Range Tuning Through Guide RNA Diversion
N Biotechnol. 2023 Jul 6:S1871-6784(23)00035-3. doi: 10.1016/j.nbt.2023.07.001. Online ahead of print.ABSTRACTCRISPRi is a powerful technique to repress gene expression in a targeted and highly efficient manner. However, this potency is a double-edged sword in inducible systems, as even leaky expression of guide RNA results in a repression phenotype, complicating applications such as dynamic metabolic engineering. We evaluated three methods to enhance the controllability of CRISPRi by modulating the level of free and DNA-bound guide RNA complexes. Overall repression can be attenuated through rationally designed mismatches ...
Source: New Biotechnology - July 8, 2023 Category: Biotechnology Authors: Bob Van Hove Lien De Wannemaeker Isolde Missiaen Jo Maertens Marjan De Mey Source Type: research
Taming CRISPRi: Dynamic Range Tuning Through Guide RNA Diversion
N Biotechnol. 2023 Jul 6:S1871-6784(23)00035-3. doi: 10.1016/j.nbt.2023.07.001. Online ahead of print.ABSTRACTCRISPRi is a powerful technique to repress gene expression in a targeted and highly efficient manner. However, this potency is a double-edged sword in inducible systems, as even leaky expression of guide RNA results in a repression phenotype, complicating applications such as dynamic metabolic engineering. We evaluated three methods to enhance the controllability of CRISPRi by modulating the level of free and DNA-bound guide RNA complexes. Overall repression can be attenuated through rationally designed mismatches ...
Source: New Biotechnology - July 8, 2023 Category: Biotechnology Authors: Bob Van Hove Lien De Wannemaeker Isolde Missiaen Jo Maertens Marjan De Mey Source Type: research
Taming CRISPRi: Dynamic Range Tuning Through Guide RNA Diversion
N Biotechnol. 2023 Jul 6:S1871-6784(23)00035-3. doi: 10.1016/j.nbt.2023.07.001. Online ahead of print.ABSTRACTCRISPRi is a powerful technique to repress gene expression in a targeted and highly efficient manner. However, this potency is a double-edged sword in inducible systems, as even leaky expression of guide RNA results in a repression phenotype, complicating applications such as dynamic metabolic engineering. We evaluated three methods to enhance the controllability of CRISPRi by modulating the level of free and DNA-bound guide RNA complexes. Overall repression can be attenuated through rationally designed mismatches ...
Source: New Biotechnology - July 8, 2023 Category: Biotechnology Authors: Bob Van Hove Lien De Wannemaeker Isolde Missiaen Jo Maertens Marjan De Mey Source Type: research
Taming CRISPRi: Dynamic Range Tuning Through Guide RNA Diversion
N Biotechnol. 2023 Jul 6:S1871-6784(23)00035-3. doi: 10.1016/j.nbt.2023.07.001. Online ahead of print.ABSTRACTCRISPRi is a powerful technique to repress gene expression in a targeted and highly efficient manner. However, this potency is a double-edged sword in inducible systems, as even leaky expression of guide RNA results in a repression phenotype, complicating applications such as dynamic metabolic engineering. We evaluated three methods to enhance the controllability of CRISPRi by modulating the level of free and DNA-bound guide RNA complexes. Overall repression can be attenuated through rationally designed mismatches ...
Source: New Biotechnology - July 8, 2023 Category: Biotechnology Authors: Bob Van Hove Lien De Wannemaeker Isolde Missiaen Jo Maertens Marjan De Mey Source Type: research
Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C < sub > 5 < /sub > and C < sub > 6 < /sub > sugars obtained from hardwood hydrolysis
N Biotechnol. 2023 Jun 28;77:40-49. doi: 10.1016/j.nbt.2023.06.005. Online ahead of print.ABSTRACTGlucose and xylose are fermentable sugars readily available from lignocellulosic biomass, and are a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work - Paraburkholderia sacchari, Hydrogenophaga pseudoflava, and Bacillus megaterium - for their ability to uptake both C5 and C6 sugars contained in a hardwood hydrolysate produced via a thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch conditions, B. meg...
Source: New Biotechnology - June 30, 2023 Category: Biotechnology Authors: Warren Blunt Purnank Shah Vinicio Vasquez Mengwei Ye Christopher Doyle Yali Liu Sajjad Saeidlou Fanny Monteil-Rivera Source Type: research
Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C < sub > 5 < /sub > and C < sub > 6 < /sub > sugars obtained from hardwood hydrolysis
N Biotechnol. 2023 Jun 28;77:40-49. doi: 10.1016/j.nbt.2023.06.005. Online ahead of print.ABSTRACTGlucose and xylose are fermentable sugars readily available from lignocellulosic biomass, and are a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work - Paraburkholderia sacchari, Hydrogenophaga pseudoflava, and Bacillus megaterium - for their ability to uptake both C5 and C6 sugars contained in a hardwood hydrolysate produced via a thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch conditions, B. meg...
Source: New Biotechnology - June 30, 2023 Category: Biotechnology Authors: Warren Blunt Purnank Shah Vinicio Vasquez Mengwei Ye Christopher Doyle Yali Liu Sajjad Saeidlou Fanny Monteil-Rivera Source Type: research
Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C < sub > 5 < /sub > and C < sub > 6 < /sub > sugars obtained from hardwood hydrolysis
N Biotechnol. 2023 Jun 28;77:40-49. doi: 10.1016/j.nbt.2023.06.005. Online ahead of print.ABSTRACTGlucose and xylose are fermentable sugars readily available from lignocellulosic biomass, and are a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work - Paraburkholderia sacchari, Hydrogenophaga pseudoflava, and Bacillus megaterium - for their ability to uptake both C5 and C6 sugars contained in a hardwood hydrolysate produced via a thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch conditions, B. meg...
Source: New Biotechnology - June 30, 2023 Category: Biotechnology Authors: Warren Blunt Purnank Shah Vinicio Vasquez Mengwei Ye Christopher Doyle Yali Liu Sajjad Saeidlou Fanny Monteil-Rivera Source Type: research
Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C < sub > 5 < /sub > and C < sub > 6 < /sub > sugars obtained from hardwood hydrolysis
N Biotechnol. 2023 Jun 28;77:40-49. doi: 10.1016/j.nbt.2023.06.005. Online ahead of print.ABSTRACTGlucose and xylose are fermentable sugars readily available from lignocellulosic biomass, and are a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work - Paraburkholderia sacchari, Hydrogenophaga pseudoflava, and Bacillus megaterium - for their ability to uptake both C5 and C6 sugars contained in a hardwood hydrolysate produced via a thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch conditions, B. meg...
Source: New Biotechnology - June 30, 2023 Category: Biotechnology Authors: Warren Blunt Purnank Shah Vinicio Vasquez Mengwei Ye Christopher Doyle Yali Liu Sajjad Saeidlou Fanny Monteil-Rivera Source Type: research
Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C < sub > 5 < /sub > and C < sub > 6 < /sub > sugars obtained from hardwood hydrolysis
N Biotechnol. 2023 Jun 28;77:40-49. doi: 10.1016/j.nbt.2023.06.005. Online ahead of print.ABSTRACTGlucose and xylose are fermentable sugars readily available from lignocellulosic biomass, and are a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work - Paraburkholderia sacchari, Hydrogenophaga pseudoflava, and Bacillus megaterium - for their ability to uptake both C5 and C6 sugars contained in a hardwood hydrolysate produced via a thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch conditions, B. meg...
Source: New Biotechnology - June 30, 2023 Category: Biotechnology Authors: Warren Blunt Purnank Shah Vinicio Vasquez Mengwei Ye Christopher Doyle Yali Liu Sajjad Saeidlou Fanny Monteil-Rivera Source Type: research
Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C < sub > 5 < /sub > and C < sub > 6 < /sub > sugars obtained from hardwood hydrolysis
N Biotechnol. 2023 Jun 28;77:40-49. doi: 10.1016/j.nbt.2023.06.005. Online ahead of print.ABSTRACTGlucose and xylose are fermentable sugars readily available from lignocellulosic biomass, and are a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work - Paraburkholderia sacchari, Hydrogenophaga pseudoflava, and Bacillus megaterium - for their ability to uptake both C5 and C6 sugars contained in a hardwood hydrolysate produced via a thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch conditions, B. meg...
Source: New Biotechnology - June 30, 2023 Category: Biotechnology Authors: Warren Blunt Purnank Shah Vinicio Vasquez Mengwei Ye Christopher Doyle Yali Liu Sajjad Saeidlou Fanny Monteil-Rivera Source Type: research
Biosynthesis and properties of polyhydroxyalkanoates synthesized from mixed C < sub > 5 < /sub > and C < sub > 6 < /sub > sugars obtained from hardwood hydrolysis
N Biotechnol. 2023 Jun 28:S1871-6784(23)00034-1. doi: 10.1016/j.nbt.2023.06.005. Online ahead of print.ABSTRACTGlucose and xylose are fermentable sugars readily available from lignocellulosic biomass, and are a sustainable carbon substrate supporting industrial biotechnology. Three strains were assessed in this work - Paraburkholderia sacchari, Hydrogenophaga pseudoflava, and Bacillus megaterium - for their ability to uptake both C5 and C6 sugars contained in a hardwood hydrolysate produced via a thermomechanical pulping-based process with concomitant production of poly(3-hydroxyalkanoate) (PHA) biopolymers. In batch condi...
Source: New Biotechnology - June 30, 2023 Category: Biotechnology Authors: Warren Blunt Purnank Shah Vinicio Vasquez Mengwei Ye Christopher Doyle Yali Liu Sajjad Saeidlou Fanny Monteil-Rivera Source Type: research
Crossing bacterial boundaries: The carbon catabolite repression system Crc-Hfq of Pseudomonas putida KT2440 as a tool to control translation in E. coli
This study reveals the feasibility of controlling gene expression using heterologous regulation systems.PMID:37348756 | DOI:10.1016/j.nbt.2023.06.004 (Source: New Biotechnology)
Source: New Biotechnology - June 22, 2023 Category: Biotechnology Authors: Chunzhe Lu Tiago P Ramalho Markus M M Bisschops Rene H Wijffels Vitor A P Martins Dos Santos Ruud A Weusthuis Source Type: research
