The stressostat: A novel approach in adaptive laboratory evolution to improve end-product resistance
In conclusion, stressostat cultivation is a valuable method to obtain L. lactis variants with improved end-product resistance and further characterization is needed to elucidate underlying resistance mechanisms and potential industrial applications.PMID:37879567 | DOI:10.1016/j.nbt.2023.10.010 (Source: New Biotechnology)
Source: New Biotechnology - October 25, 2023 Category: Biotechnology Authors: Sylviani Hartono Marlisa F A Meijerink Tjakko Abee Eddy J Smid Oscar van Mastrigt Source Type: research

The stressostat: A novel approach in adaptive laboratory evolution to improve end-product resistance
In conclusion, stressostat cultivation is a valuable method to obtain L. lactis variants with improved end-product resistance and further characterization is needed to elucidate underlying resistance mechanisms and potential industrial applications.PMID:37879567 | DOI:10.1016/j.nbt.2023.10.010 (Source: New Biotechnology)
Source: New Biotechnology - October 25, 2023 Category: Biotechnology Authors: Sylviani Hartono Marlisa F A Meijerink Tjakko Abee Eddy J Smid Oscar van Mastrigt Source Type: research

The stressostat: A novel approach in adaptive laboratory evolution to improve end-product resistance
In conclusion, stressostat cultivation is a valuable method to obtain L. lactis variants with improved end-product resistance and further characterization is needed to elucidate underlying resistance mechanisms and potential industrial applications.PMID:37879567 | DOI:10.1016/j.nbt.2023.10.010 (Source: New Biotechnology)
Source: New Biotechnology - October 25, 2023 Category: Biotechnology Authors: Sylviani Hartono Marlisa F A Meijerink Tjakko Abee Eddy J Smid Oscar van Mastrigt Source Type: research

The stressostat: a novel approach in adaptive laboratory evolution to improve end-product resistance
In conclusion, stressostat cultivation is a valuable method to obtain L. lactis variants with improved end-product resistance and further characterization is needed to elucidate underlying resistance mechanisms and potential industrial applications.PMID:37879567 | DOI:10.1016/j.nbt.2023.10.010 (Source: New Biotechnology)
Source: New Biotechnology - October 25, 2023 Category: Biotechnology Authors: Sylviani Hartono Marlisa F A Meijerink Tjakko Abee Eddy J Smid Oscar van Mastrigt Source Type: research

The stressostat: a novel approach in adaptive laboratory evolution to improve end-product resistance
In conclusion, stressostat cultivation is a valuable method to obtain L. lactis variants with improved end-product resistance and further characterization is needed to elucidate underlying resistance mechanisms and potential industrial applications.PMID:37879567 | DOI:10.1016/j.nbt.2023.10.010 (Source: New Biotechnology)
Source: New Biotechnology - October 25, 2023 Category: Biotechnology Authors: Sylviani Hartono Marlisa F A Meijerink Tjakko Abee Eddy J Smid Oscar van Mastrigt Source Type: research

Effects of municipal waste compost on microbial biodiversity and energy production in terrestrial microbial fuel cells
N Biotechnol. 2023 Oct 22:S1871-6784(23)00060-2. doi: 10.1016/j.nbt.2023.10.009. Online ahead of print.ABSTRACTMicrobial Fuel Cells (MFCs) transform organic matter into electricity through microbial electrochemical reactions catalysed on anodic and cathodic half-cells. Terrestrial MFCs (TMFCs) are a bioelectrochemical system for bioelectricity production as well as soil remediation. In TMFCs, the soil is the ion-exchange electrolyte, whereas a biofilm on the anode oxidises organic matter through electroactive bacteria. Little is known of the overall microbial community composition in a TMFC, which impedes complete exploita...
Source: New Biotechnology - October 24, 2023 Category: Biotechnology Authors: Gian Luigi Garbini Anna Barra Caracciolo Ludovica Rolando Andrea Visca Domenico Borello Carlotta Cosentini Gabriele Gagliardi Ioannis Ieropoulos Paola Grenni Source Type: research

Effects of municipal waste compost on microbial biodiversity and energy production in terrestrial microbial fuel cells
N Biotechnol. 2023 Oct 22:S1871-6784(23)00060-2. doi: 10.1016/j.nbt.2023.10.009. Online ahead of print.ABSTRACTMicrobial Fuel Cells (MFCs) transform organic matter into electricity through microbial electrochemical reactions catalysed on anodic and cathodic half-cells. Terrestrial MFCs (TMFCs) are a bioelectrochemical system for bioelectricity production as well as soil remediation. In TMFCs, the soil is the ion-exchange electrolyte, whereas a biofilm on the anode oxidises organic matter through electroactive bacteria. Little is known of the overall microbial community composition in a TMFC, which impedes complete exploita...
Source: New Biotechnology - October 24, 2023 Category: Biotechnology Authors: Gian Luigi Garbini Anna Barra Caracciolo Ludovica Rolando Andrea Visca Domenico Borello Carlotta Cosentini Gabriele Gagliardi Ioannis Ieropoulos Paola Grenni Source Type: research

Electrochemical H < sub > 2 < /sub > O < sub > 2 < /sub > - stat mode as reaction concept to improve the process performance of an unspecific peroxygenase
In this study, two in-situ electrogeneration modes of H2O2 were established and compared. Experiments under galvanostatic conditions (constant productivity of H2O2) were conducted at current densities spanning from 0.8 mA cm-2 to 6.4 mA cm-2. For comparison, experiments under H2O2-stat mode (constant H2O2 concentration) were performed. Here, four H2O2 concentrations between 0.06 mM and 0.28 mM were tested. A maximum H2O2 productivity of 5.5 µM min-1 cm-2 and productivity of 10.5 g L-1 d-1 were achieved under the galvanostatic condition at 6.4 mA cm-2. Meanwhile, the highest total turnover number (TTN) of 710,000 mol mol-1...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Giovanni V Sayoga Victoria S Bueschler Hubert Beisch Tyll Utesch Dirk Holtmann Bodo Fiedler Daniel Ohde Andreas Liese Source Type: research

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria
N Biotechnol. 2023 Oct 16:S1871-6784(23)00056-0. doi: 10.1016/j.nbt.2023.10.008. Online ahead of print.ABSTRACTSeven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) production, both alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and opti...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Beatriz Altamira-Algarra Estel Rueda Artai Lage David San Le ón Juan F Mart ínez-Blanch Juan Nogales Joan Garc ía Eva Gonzalez-Flo Source Type: research

Electrochemical H < sub > 2 < /sub > O < sub > 2 < /sub > - stat mode as reaction concept to improve the process performance of an unspecific peroxygenase
In this study, two in-situ electrogeneration modes of H2O2 were established and compared. Experiments under galvanostatic conditions (constant productivity of H2O2) were conducted at current densities spanning from 0.8 mA cm-2 to 6.4 mA cm-2. For comparison, experiments under H2O2-stat mode (constant H2O2 concentration) were performed. Here, four H2O2 concentrations between 0.06 mM and 0.28 mM were tested. A maximum H2O2 productivity of 5.5 µM min-1 cm-2 and productivity of 10.5 g L-1 d-1 were achieved under the galvanostatic condition at 6.4 mA cm-2. Meanwhile, the highest total turnover number (TTN) of 710,000 mol mol-1...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Giovanni V Sayoga Victoria S Bueschler Hubert Beisch Tyll Utesch Dirk Holtmann Bodo Fiedler Daniel Ohde Andreas Liese Source Type: research

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria
N Biotechnol. 2023 Oct 16:S1871-6784(23)00056-0. doi: 10.1016/j.nbt.2023.10.008. Online ahead of print.ABSTRACTSeven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) production, both alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and opti...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Beatriz Altamira-Algarra Estel Rueda Artai Lage David San Le ón Juan F Mart ínez-Blanch Juan Nogales Joan Garc ía Eva Gonzalez-Flo Source Type: research

Electrochemical H < sub > 2 < /sub > O < sub > 2 < /sub > - stat mode as reaction concept to improve the process performance of an unspecific peroxygenase
In this study, two in-situ electrogeneration modes of H2O2 were established and compared. Experiments under galvanostatic conditions (constant productivity of H2O2) were conducted at current densities spanning from 0.8 mA cm-2 to 6.4 mA cm-2. For comparison, experiments under H2O2-stat mode (constant H2O2 concentration) were performed. Here, four H2O2 concentrations between 0.06 mM and 0.28 mM were tested. A maximum H2O2 productivity of 5.5 µM min-1 cm-2 and productivity of 10.5 g L-1 d-1 were achieved under the galvanostatic condition at 6.4 mA cm-2. Meanwhile, the highest total turnover number (TTN) of 710,000 mol mol-1...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Giovanni V Sayoga Victoria S Bueschler Hubert Beisch Tyll Utesch Dirk Holtmann Bodo Fiedler Daniel Ohde Andreas Liese Source Type: research

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria
N Biotechnol. 2023 Oct 16:S1871-6784(23)00056-0. doi: 10.1016/j.nbt.2023.10.008. Online ahead of print.ABSTRACTSeven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) production, both alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and opti...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Beatriz Altamira-Algarra Estel Rueda Artai Lage David San Le ón Juan F Mart ínez-Blanch Juan Nogales Joan Garc ía Eva Gonzalez-Flo Source Type: research

Electrochemical H < sub > 2 < /sub > O < sub > 2 < /sub > - stat mode as reaction concept to improve the process performance of an unspecific peroxygenase
In this study, two in-situ electrogeneration modes of H2O2 were established and compared. Experiments under galvanostatic conditions (constant productivity of H2O2) were conducted at current densities spanning from 0.8mAcm-2 to 6.4mAcm-2. For comparison, experiments under H2O2-stat mode (constant H2O2 concentration) were performed. Here, four H2O2 concentrations between 0.06mM and 0.28mM were tested. A maximum H2O2 productivity of 5.5µMmin-1 cm-2 and productivity of 10.5gL-1 d-1 were achieved under the galvanostatic condition at 6.4mAcm-2. Meanwhile, the highest total turnover number (TTN) of 710,000molmol-1 and turnover ...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Giovanni V Sayoga Victoria S Bueschler Hubert Beisch Tyll Utesch Dirk Holtmann Bodo Fiedler Daniel Ohde Andreas Liese Source Type: research

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria
N Biotechnol. 2023 Oct 16:S1871-6784(23)00056-0. doi: 10.1016/j.nbt.2023.10.008. Online ahead of print.ABSTRACTSeven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) production, both alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and opti...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Beatriz Altamira-Algarra Estel Rueda Artai Lage David San Le ón Juan F Mart ínez-Blanch Juan Nogales Joan Garc ía Eva Gonzalez-Flo Source Type: research