Electrochemical H < sub > 2 < /sub > O < sub > 2 < /sub > - stat mode as reaction concept to improve the process performance of an unspecific peroxygenase
In this study, two in-situ electrogeneration modes of H2O2 were established and compared. Experiments under galvanostatic conditions (constant productivity of H2O2) were conducted at current densities spanning from 0.8mAcm-2 to 6.4mAcm-2. For comparison, experiments under H2O2-stat mode (constant H2O2 concentration) were performed. Here, four H2O2 concentrations between 0.06mM and 0.28mM were tested. A maximum H2O2 productivity of 5.5µMmin-1 cm-2 and productivity of 10.5gL-1 d-1 were achieved under the galvanostatic condition at 6.4mAcm-2. Meanwhile, the highest total turnover number (TTN) of 710,000molmol-1 and turnover ...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Giovanni V Sayoga Victoria S Bueschler Hubert Beisch Tyll Utesch Dirk Holtmann Bodo Fiedler Daniel Ohde Andreas Liese Source Type: research
New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria
N Biotechnol. 2023 Oct 16:S1871-6784(23)00056-0. doi: 10.1016/j.nbt.2023.10.008. Online ahead of print.ABSTRACTSeven photosynthethic microbiomes were collected from field environmental samples to test their potential in polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) production, both alternatives to chemical-based polymers. Microscope observations together with microbial sequence analysis revealed the microbiome enrichment in cyanobacteria after culture growth under phosphorus limitation. PHB and EPS production were studied under three culture factors (phototrophy, mixotrophy and heterotrophy) by evaluating and opti...
Source: New Biotechnology - October 18, 2023 Category: Biotechnology Authors: Beatriz Altamira-Algarra Estel Rueda Artai Lage David San Le ón Juan F Mart ínez-Blanch Juan Nogales Joan Garc ía Eva Gonzalez-Flo Source Type: research
Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content
N Biotechnol. 2023 Oct 15:S1871-6784(23)00057-2. doi: 10.1016/j.nbt.2023.10.005. Online ahead of print.ABSTRACTThe halotolerant non-conventional yeast Debaryomyces hansenii can grow in media containing high concentrations of salt (up to 4M), metabolize alternative carbon sources than glucose, such as lactose or glycerol, and withstand a wide range of temperatures and pH. These inherent capabilities allow this yeast to grow in harsh environments and use alternative feedstock than traditional commercial media. For example, D. hansenii could be a potential cell factory for revalorizing industrial salty by-products, using them...
Source: New Biotechnology - October 17, 2023 Category: Biotechnology Authors: M ònica Estrada Clara Navarrete S ønke Møller Manuel Quir ós Jos é L Martínez Source Type: research
Personalized 3D printed scaffolds: the ethical aspects
This article provides an overview of these relevant ethical aspects. We identified nine themes which were linked to three stages of the life cycle of the scaffold: the development process, clinical testing, and the implementation process. The described ethical issues are related to good research and clinical practices, such as privacy issues concerning digitalization, first-in-human trials, responsibility and commercialization. At the same time, this article also creates awareness for underexplored ethical issues, such as irreversibility, embodiment and the ontological status of these scaffolds. Moreover, it exemplifies ho...
Source: New Biotechnology - October 17, 2023 Category: Biotechnology Authors: Manon van Daal Anne-Floor de Kanter Annelien Bredenoord Nienke de Graeff Source Type: research
Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content
N Biotechnol. 2023 Oct 15:S1871-6784(23)00057-2. doi: 10.1016/j.nbt.2023.10.005. Online ahead of print.ABSTRACTThe halotolerant non-conventional yeast Debaryomyces hansenii can grow in media containing high concentrations of salt (up to 4M), metabolize alternative carbon sources than glucose, such as lactose or glycerol, and withstand a wide range of temperatures and pH. These inherent capabilities allow this yeast to grow in harsh environments and use alternative feedstock than traditional commercial media. For example, D. hansenii could be a potential cell factory for revalorizing industrial salty by-products, using them...
Source: New Biotechnology - October 17, 2023 Category: Biotechnology Authors: M ònica Estrada Clara Navarrete S ønke Møller Manuel Quir ós Jos é L Martínez Source Type: research
Personalized 3D printed scaffolds: the ethical aspects
This article provides an overview of these relevant ethical aspects. We identified nine themes which were linked to three stages of the life cycle of the scaffold: the development process, clinical testing, and the implementation process. The described ethical issues are related to good research and clinical practices, such as privacy issues concerning digitalization, first-in-human trials, responsibility and commercialization. At the same time, this article also creates awareness for underexplored ethical issues, such as irreversibility, embodiment and the ontological status of these scaffolds. Moreover, it exemplifies ho...
Source: New Biotechnology - October 17, 2023 Category: Biotechnology Authors: Manon van Daal Anne-Floor de Kanter Annelien Bredenoord Nienke de Graeff Source Type: research
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source
This study investigated the potential for the production of mcl-PHA from volatile fatty acids (VFA) at a larger scale, given their potential as low-cost and sustainable raw material within a carboxylate-platform based biorefinery. Pseudomonas citronellolis (DSMZ 50332) was chosen as the production strain, and acetic acid was selected as the main carbon and energy source. Nitrogen was limited to trigger polymer production, and a fed-batch process using a pH-stat feeding regime with concentrated acid was established. We report successful production, extraction, and characterization of mcl PHA, obtaining a total of 1.76kg fro...
Source: New Biotechnology - October 12, 2023 Category: Biotechnology Authors: Milos Kacanski Franz Stelzer Meg Walsh Shane Kenny Kevin O'Connor Markus Neureiter Source Type: research
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source
This study investigated the potential for the production of mcl-PHA from volatile fatty acids (VFA) at a larger scale, given their potential as low-cost and sustainable raw material within a carboxylate-platform based biorefinery. Pseudomonas citronellolis (DSMZ 50332) was chosen as the production strain, and acetic acid was selected as the main carbon and energy source. Nitrogen was limited to trigger polymer production, and a fed-batch process using a pH-stat feeding regime with concentrated acid was established. We report successful production, extraction, and characterization of mcl PHA, obtaining a total of 1.76kg fro...
Source: New Biotechnology - October 12, 2023 Category: Biotechnology Authors: Milos Kacanski Franz Stelzer Meg Walsh Shane Kenny Kevin O'Connor Markus Neureiter Source Type: research
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source
This study investigated the potential for the production of mcl-PHA from volatile fatty acids (VFA) at a larger scale, given their potential as low-cost and sustainable raw material within a carboxylate-platform based biorefinery. Pseudomonas citronellolis (DSMZ 50332) was chosen as the production strain, and acetic acid was selected as the main carbon and energy source. Nitrogen was limited to trigger polymer production, and a fed-batch process using a pH-stat feeding regime with concentrated acid was established. We report successful production, extraction, and characterization of mcl PHA, obtaining a total of 1.76kg fro...
Source: New Biotechnology - October 12, 2023 Category: Biotechnology Authors: Milos Kacanski Franz Stelzer Meg Walsh Shane Kenny Kevin O'Connor Markus Neureiter Source Type: research
Pilot-scale production of mcl-PHA by Pseudomonas citronellolis using acetic acid as the sole carbon source
This study investigated the potential for the production of mcl-PHA from volatile fatty acids (VFA) at a larger scale, given their potential as low-cost and sustainable raw material within a carboxylate-platform based biorefinery. Pseudomonas citronellolis (DSMZ 50332) was chosen as the production strain, and acetic acid was selected as the main carbon and energy source. Nitrogen was limited to trigger polymer production, and a fed-batch process using a pH-stat feeding regime with concentrated acid was established. We report successful production, extraction, and characterization of mcl PHA, obtaining a total of 1.76kg fro...
Source: New Biotechnology - October 12, 2023 Category: Biotechnology Authors: Milos Kacanski Franz Stelzer Meg Walsh Shane Kenny Kevin O'Connor Markus Neureiter Source Type: research
Site-specific antibody masking enables conditional activation with different stimuli
N Biotechnol. 2023 Oct 9:S1871-6784(23)00054-7. doi: 10.1016/j.nbt.2023.10.004. Online ahead of print.ABSTRACTAntibody therapeutics show great potential to treat a variety of diseases. Often, the dose that can be safely administered is limited by the onset of side effects that arise from the interaction with the target outside the diseased tissue. Conditionally-active antibodies provide an additional layer of selectivity to improve safety. Distinct external stimuli or internal cues enable different control strategies and applications. However, current antibody masking strategies have low transferability across stimuli. Her...
Source: New Biotechnology - October 11, 2023 Category: Biotechnology Authors: Roberta Lucchi Maria C Lucana Montserrat Escobar-Rosales Cristina D íaz-Perlas Benjam í Oller-Salvia Source Type: research
Microalgae Harvesting for Wastewater Treatment and Resources Recovery: A Review
N Biotechnol. 2023 Oct 9:S1871-6784(23)00053-5. doi: 10.1016/j.nbt.2023.10.002. Online ahead of print.ABSTRACTMicroalgae-based wastewater treatment has been conceived to obtain reclaimed water and produce microalgal biomass for bio-based products and biofuels generation. However, microalgal biomass harvesting is challenging and expensive, hence one of the main bottlenecks for full-scale implementation. Finding an integrated approach that covers concepts of engineering, green chemistry and the application of microbial anabolism driven towards the harvesting processes, is mandatory for the widespread establishment of full-sc...
Source: New Biotechnology - October 11, 2023 Category: Biotechnology Authors: Etiele Greque de Morais Igor Carvalho Fontes Sampaio Eva Gonzalez-Flo Ivet Ferrer Enrica Uggetti Joan Garc ía Source Type: research
Site-specific antibody masking enables conditional activation with different stimuli
N Biotechnol. 2023 Oct 9:S1871-6784(23)00054-7. doi: 10.1016/j.nbt.2023.10.004. Online ahead of print.ABSTRACTAntibody therapeutics show great potential to treat a variety of diseases. Often, the dose that can be safely administered is limited by the onset of side effects that arise from the interaction with the target outside the diseased tissue. Conditionally-active antibodies provide an additional layer of selectivity to improve safety. Distinct external stimuli or internal cues enable different control strategies and applications. However, current antibody masking strategies have low transferability across stimuli. Her...
Source: New Biotechnology - October 11, 2023 Category: Biotechnology Authors: Roberta Lucchi Maria C Lucana Montserrat Escobar-Rosales Cristina D íaz-Perlas Benjam í Oller-Salvia Source Type: research
Microalgae Harvesting for Wastewater Treatment and Resources Recovery: A Review
N Biotechnol. 2023 Oct 9:S1871-6784(23)00053-5. doi: 10.1016/j.nbt.2023.10.002. Online ahead of print.ABSTRACTMicroalgae-based wastewater treatment has been conceived to obtain reclaimed water and produce microalgal biomass for bio-based products and biofuels generation. However, microalgal biomass harvesting is challenging and expensive, hence one of the main bottlenecks for full-scale implementation. Finding an integrated approach that covers concepts of engineering, green chemistry and the application of microbial anabolism driven towards the harvesting processes, is mandatory for the widespread establishment of full-sc...
Source: New Biotechnology - October 11, 2023 Category: Biotechnology Authors: Etiele Greque de Morais Igor Carvalho Fontes Sampaio Eva Gonzalez-Flo Ivet Ferrer Enrica Uggetti Joan Garc ía Source Type: research
Droplet digital PCR: A comprehensive tool for genetic analysis and prediction of bispecific antibody assembly during cell line development
In this study, the diverse applications of droplet digital PCR (ddPCR) as a molecular biological tool for cell line development are demonstrated. Specifically, it is shown that ddPCR can be used to enable precise, sensitive and reproducible absolute quantification of genomically integrated transgene copies during cell line development and cell bank characterization. Additionally, an amplitude multiplexing approach is applied to simultaneously run multiple assays on different genetic targets in a single reaction and advance clonal screening by measuring gene expression profiles to predict the assembly and homogeneity of dif...
Source: New Biotechnology - October 5, 2023 Category: Biotechnology Authors: Daniel Heinzelmann Benjamin Lindner Benjamin Renner Simon Fischer Patrick Schulz Moritz Schmidt Source Type: research
