Approaches for High-throughput Quantification of Periplasmic Recombinant Proteins

N Biotechnol. 2023 Sep 12:S1871-6784(23)00046-8. doi: 10.1016/j.nbt.2023.09.003. Online ahead of print.ABSTRACTThe Gram-negative periplasm is a convenient location for the accumulation of many recombinant proteins including biopharmaceutical products. It is the site of disulphide bond formation, required by some proteins (such as antibody fragments) for correct folding and function. It also permits simpler protein release and downstream processing than cytoplasmic accumulation. As such, targeting of recombinant proteins to the E. coli periplasm is a key strategy in biologic manufacture. However, expression and translocation of each recombinant protein requires optimisation including selection of the best signal peptide and growth and production conditions. Traditional methods require separation and analysis of protein compositions of periplasmic and cytoplasmic fractions, a time- and labour-intensive method that is difficult to parallelise. Therefore, approaches for high throughput quantification of periplasmic protein accumulation offer advantages in rapid process development.PMID:37708933 | DOI:10.1016/j.nbt.2023.09.003
Source: New Biotechnology - Category: Biotechnology Authors: Source Type: research