B-Glycine as a marker for β cell imaging and β cell mass evaluation
CONCLUSION: [18F]FBG targeting the endogenous glycine transporter (GLYT), which is highly expressed on islet cells, avoiding extra modification on islet cells. Meanwhile the highly restricted expression pattern of GLYT excluded the background in adjacent tissues. This [18F]FBG-based imaging technique provides a non-invasive method to quantify BCM in vivo, implying a new evaluation index for diabetic assessment.PMID:38632133 | DOI:10.1007/s00259-024-06712-y (Source: Molecular Medicine)
Source: Molecular Medicine - April 17, 2024 Category: Molecular Biology Authors: Yuxiang Han Hui Liu Yimin Li Zhibo Liu Source Type: research
HS-AFM single-molecule structural biology uncovers basis of transporter wanderlust kinetics
Nat Struct Mol Biol. 2024 Apr 17. doi: 10.1038/s41594-024-01260-3. Online ahead of print.ABSTRACTThe Pyrococcus horikoshii amino acid transporter GltPh revealed, like other channels and transporters, activity mode switching, previously termed wanderlust kinetics. Unfortunately, to date, the basis of these activity fluctuations is not understood, probably due to a lack of experimental tools that directly access the structural features of transporters related to their instantaneous activity. Here, we take advantage of high-speed atomic force microscopy, unique in providing simultaneous structural and temporal resolution, to ...
Source: Molecular Medicine - April 17, 2024 Category: Molecular Biology Authors: Yining Jiang Atsushi Miyagi Xiaoyu Wang Biao Qiu Olga Boudker Simon Scheuring Source Type: research
The plasma proteome differentiates the multisystem inflammatory syndrome in children (MIS-C) from children with SARS-CoV-2 negative sepsis
CONCLUSIONS: The plasma proteome of MIS-C patients was distinct from that of SCNS. The key proteins demonstrated expression in all organ systems and most cell types. The unique proteomic signature identified in MIS-C patients could aid future diagnostic and therapeutic advancements, as well as predict hospital length of stays, interventions, and mortality risks.PMID:38632526 | DOI:10.1186/s10020-024-00806-x (Source: Molecular Medicine)
Source: Molecular Medicine - April 17, 2024 Category: Molecular Biology Authors: Maitray A Patel Douglas D Fraser Mark Daley Gediminas Cepinskas Noemi Veraldi Serge Grazioli Source Type: research
Pre- and Postembedding Immunoelectron Microscopy to Analyze the Central Nervous System
Methods Mol Biol. 2024;2794:45-62. doi: 10.1007/978-1-0716-3810-1_5.ABSTRACTImmunocytochemistry, a method of delineating the subcellular localization of target proteins, was developed from immunohistochemistry. In principle, proteins are labeled using an antigen-antibody reaction. In order to observe under an electron microscope, the reaction product must scatter the electron beam with sufficient contrast while it is necessary to have an amplifying label that can withstand the observation. We have some detailed tips on making electron microscope samples to achieve this objective, and we would be happy to help you.PMID:3863...
Source: Molecular Medicine - April 17, 2024 Category: Molecular Biology Authors: Fumino Fujiyama Fuyuki Karube Source Type: research
Chlordiazepoxide against signalling, receptor and regulatory proteins of breast cancer: a structure-based in-silico approach
In this study, we included four crucial proteins that perform signalling, receptor, and regulatory action, namely- NUDIX Hydrolases, Dihydrofolate Reductase, HER2/neu Kinase and EGFR and performed multitargeted molecular docking studies against human-approved drugs using HTVS, SP and extra precise algorithms and filtered the poses with MM\GBSA, suggested a benzodiazepine derivative chlordiazepoxide, used as an anxiolytic agent, can be a multitargeted inhibitor with docking and MM\GBSA score ranging from - 4.628 to - 7.877 and - 18.59 to - 135.86 kcal/mol, respectively, and the most interacted residues were 6ARG, 6GLU, 3TRP...
Source: Molecular Medicine - April 17, 2024 Category: Molecular Biology Authors: Ahad Amer Alsaiari Amal F Gharib Maha Mahfouz Bakhuraysah Amani A Alrehaili Shatha M Algethami Hayfa Ali Alsaif Norah Al Harthi Mohammed Ageeli Hakami Source Type: research
Genetic analysis of nephrogenic diabetes insipidus patients: A study on the Iranian population
In this study, 6 different mutations were identified in the patients, including 5 in the AQP2 gene (c.439G>A, c.538G>A, c.140C>T, c.450T>A, and the novel c.668T>C) and 1 in the AVPR2 gene (c.337C>T) in the present study.DISCUSSION: As expected, all the detected mutations in this study were missense. According to the ACMG guideline, the identified mutations were categorized as pathogenic or likely pathogenic. Unlike previous studies which showed more than 90% of mutations were in the AVPR2 gene, and only less than 10% of the mutations were in the AQP2 gene, it was found that more than 90% of our identified...
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Saeed Ghasemi Marzieh Mojbafan Saeed Talebi Nakysa Hooman Rozita Hoseini Source Type: research
Congenital disorders of glycosylation with multiorgan disruption and immune dysregulation caused by compound heterozygous variants in MAN2B2
CONCLUSION: We described a CDG patient with novel phenotypes and disruptive N-glycan profiling caused by compound heterozygous MAN2B2 variants (c.384G>T; c.926T>A). Our findings broadened both the genetic and clinical spectra of CDG.PMID:38622837 | DOI:10.1002/mgg3.2422 (Source: Molecular Medicine)
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Shiqi Fan Huanhuan Wu Rongrong Wang Qian Chen Xue Zhang Source Type: research
Reclassification of a spindle cell sarcoma after identification of a TFG-ROS1 fusion: A case demonstrating the clinical benefit of next-generation sequencing in sarcoma
CONCLUSIONS: We discuss the role of NGS as well as its potential benefit in patients with unresectable, ALK-negative metastatic disease. Considering this case and previous literature, we support the use of NGS for patients requiring systemic treatment.PMID:38622850 | DOI:10.1002/mgg3.2423 (Source: Molecular Medicine)
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: John J Lim Eleanor Y Chen Stephanie K Schaub Michael J Wagner Source Type: research
Novel radiation and targeted therapy combinations for improving rectal cancer outcomes
Expert Rev Mol Med. 2024 Apr 16:1-26. doi: 10.1017/erm.2024.15. Online ahead of print.NO ABSTRACTPMID:38623751 | DOI:10.1017/erm.2024.15 (Source: Molecular Medicine)
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Kathryn Pennel Louise Dutton Lydia Melissourgou-Syka Campbell Roxburgh Joanna Birch Joanne Edwards Source Type: research
Cadherin-17 as a target for the immunoPET of adenocarcinoma
CONCLUSION: Taken together, these data underscore that [89Zr]Zr-DFO-D2101 is a highly promising probe for the non-invasive visualization of CDH17 expression in PDAC. We contend that this radioimmunoconjugate could have a significant impact on the clinical management of patients with both PDAC and gastrointestinal adenocarcinoma, most likely as a theranostic imaging tool in support of CDH17-targeted therapies.PMID:38625402 | DOI:10.1007/s00259-024-06709-7 (Source: Molecular Medicine)
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Samantha Delaney Outi Kein änen Dennis Lam Andrew L Wolfe Takao Hamakubo Brian M Zeglis Source Type: research
Delineating the contribution of ageing and physical activity to changes in mitochondrial characteristics across the lifespan
Mol Aspects Med. 2024 Apr 15;97:101272. doi: 10.1016/j.mam.2024.101272. Online ahead of print.ABSTRACTAgeing is associated with widespread physiological changes prominent within all tissues, including skeletal muscle and the brain, which lead to a decline in physical function. To tackle the growing health and economic burdens associated with an ageing population, the concept of healthy ageing has become a major research priority. Changes in skeletal muscle mitochondrial characteristics have been suggested to make an important contribution to the reductions in skeletal muscle function with age, and age-related changes in mi...
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Matthew J-C Lee Nicholas J Saner Alessandra Ferri Esther Garc ía-Domínguez James R Broatch David J Bishop Source Type: research
Exploration of isoxazole analogs: Synthesis, COX inhibition, anticancer screening, 3D multicellular tumor spheroids, and molecular modeling
In this study, a new series of Isoxazole-carboxamide derivatives were synthesized and characterized via HRMS, 1H-, 13CAPT-NMR, and MicroED. The findings revealed that nearly all of the synthesized derivatives exhibited potent inhibitory activities against both COX enzymes, with IC50 values ranging from 4.1 nM to 3.87 μM. Specifically, MYM1 demonstrated the highest efficacy among the compounds tested against the COX-1, displaying an IC50 value of 4.1 nM. The results showed that 5 compounds possess high COX-2 isozyme inhibitory effects with IC50 value in range 0.24-1.30 μM with COX-2 selectivity indexes (2.51-6.13), among ...
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: Mohammed Hawash Samer Abdallah Mahmoud Abudayyak Yarob Melhem Mohammed Abu Shamat Meera Aghbar Irfan Çapan Murad Abualhasan Anil Kumar Micha ł Kamiński Tomasz G óral Paulina Maria Dominiak Shorooq Sobuh Source Type: research
Development and validation of an alternative procedure for quantitative quality control analysis of < sup > 99 < /sup > mTc-radiopharmaceuticals using a Geiger M üller counter
CONCLUSIONS: This work demonstrates that a Geiger Müller detector with a probe for measuring surface contamination can be adequately used to replace other equipment in the control of radiochemical purity in the hospital radiopharmacy.PMID:38626842 | DOI:10.1016/j.remnie.2024.500006 (Source: Molecular Medicine)
Source: Molecular Medicine - April 16, 2024 Category: Molecular Biology Authors: M M Gonzalez A L Poma N Cuello A L Soldati Source Type: research
Harnessing gastrointestinal organoids for cancer therapy
Trends Mol Med. 2024 Apr 13:S1471-4914(24)00065-0. doi: 10.1016/j.molmed.2024.03.010. Online ahead of print.ABSTRACTGastrointestinal organoids have emerged as a model system that authentically recapitulates the in vivo situation. Despite biomedical and technical challenges, self-assembled 3D structures derived from pluripotent stem cells or healthy and diseased tissues have proved to be invaluable tools for cancer drug discovery, disease modeling, and studying infection with carcinogenic pathogens.PMID:38616435 | DOI:10.1016/j.molmed.2024.03.010 (Source: Molecular Medicine)
Source: Molecular Medicine - April 15, 2024 Category: Molecular Biology Authors: Gunter Maubach Michael Naumann Source Type: research
Clinical application of the retrograde track method in reopening dysfunctional biliary stents
CONCLUSIONS: RTM is an effective alternative treatment method when anterograde reopening of a dysfunctional biliary stent occurs.PMID:38617160 | PMC:PMC11007521 | DOI:10.21037/qims-23-1030 (Source: Molecular Medicine)
Source: Molecular Medicine - April 15, 2024 Category: Molecular Biology Authors: Rongna Hou Mengyao Song Xueliang Zhou Chengzhi Zhang Dechao Jiao Source Type: research
