The diagnostic performance of functional dopaminergic scintigraphic imaging in the diagnosis of dementia with Lewy bodies: an updated systematic review
CONCLUSION: Our systematic review confirms the major role of dopaminergic scintigraphic imaging in the assessment of dementia with Lewy bodies. Early diagnosis could be facilitated by identifying the prodromes of dementia with Lewy bodies using dopaminergic scintigraphic imaging coupled with emphasis on clinical neuropsychiatric symptoms. Most published studies use a semi-quantitative analytical assessment of tracer uptake, while there are no studies using quantitative analytical methods to measure dopamine transporter loss. The superiority of a purely quantitative approach to assess dopaminergic transmission more accurate...
Source: Molecular Medicine - March 15, 2023 Category: Molecular Biology Authors: Mario Jreige George K Kurian J érémy Perriraz Jevita Potheegadoo Fosco Bernasconi Sara Stampacchia Olaf Blanke Griffa Alessandra Noemie Lejay Paolo Salvioni Chiabotti Olivier Rouaud Marie Nicod Lalonde Niklaus Schaefer Giorgio Treglia Gilles Allali John Source Type: research
LncRNA GAS5 suppresses TGF- β1-induced transformation of pulmonary pericytes into myofibroblasts by recruiting KDM5B and promoting H3K4me2/3 demethylation of the PDGFRα/β promoter
CONCLUSION: GAS5 represses pericyte-myofibroblast transformation by inhibiting PDGFRα/β expression via KDM5B-mediated H3K4me2/3 demethylation in IPF, identifying GAS5 as an intervention target for IPF.PMID:36918759 | DOI:10.1186/s10020-023-00620-x (Source: Molecular Medicine)
Source: Molecular Medicine - March 15, 2023 Category: Molecular Biology Authors: Yichun Wang Diyu Chen Han Xie Shuhua Zhou Mingwang Jia Xiaobo He Feifei Guo Yihuan Lai Xiao Xiao Tang Source Type: research
Caveolin-1 is involved in fatty infiltration and bone-tendon healing of rotator cuff tear
CONCLUSION: Caveolin-1 was identified as a key gene involved in the RCT process. In vitro results demonstrated that Caveolin-1 knockdown inhibited adipogenic differentiation of TSCs by activating the cAMP/PKA pathway. GATA6 inhibited the transcription of Caveolin-1 and inhibited its expression, thus suppressing the adipogenic differentiation of TSCs. In vivo data confirmed that GATA6 overexpression activated the cAMP/PKA pathway by downregulating Caveolin-1 and consequently repressed fatty infiltration, promoted bone-tendon healing, improved biomechanical properties and reduced the rupture risk of injured tendon in rats af...
Source: Molecular Medicine - March 15, 2023 Category: Molecular Biology Authors: Shanhong Fang Mengqiang You Jie Wei Peng Chen Source Type: research
Resveratrol-Exemestane Beneficial Regimen to Overcome the Complications over Existing Therapies for the Management of Breast Cancer
Curr Mol Med. 2023 Mar 14. doi: 10.2174/1566524023666230314104442. Online ahead of print.NO ABSTRACTPMID:36918787 | DOI:10.2174/1566524023666230314104442 (Source: Molecular Medicine)
Source: Molecular Medicine - March 15, 2023 Category: Molecular Biology Authors: Simranjeet Kaur Dilpreet Singh Source Type: research
4-Octyl itaconate attenuates glycemic deterioration by regulating macrophage polarization in mouse models of type 1 diabetes
CONCLUSIONS: These results provided the first evidence to date that OI can prevent the progression of glycemic deterioration, excessive inflammation, and beta cell dysfunction predominantly mediated by restricting macrophage M1 polarization in mouse models of type 1 diabetes.PMID:36918798 | DOI:10.1186/s10020-023-00626-5 (Source: Molecular Medicine)
Source: Molecular Medicine - March 15, 2023 Category: Molecular Biology Authors: Sunyue He Yuchen Zhao Guoxing Wang Qiaofang Ke Nan Wu Lusi Lu Jiahua Wu Shuiya Sun Weihua Jin Wenjing Zhang Jiaqiang Zhou Source Type: research
The role of NEDD4 related HECT-type E3 ubiquitin ligases in defective autophagy in cancer cells: molecular mechanisms and therapeutic perspectives
Mol Med. 2023 Mar 14;29(1):34. doi: 10.1186/s10020-023-00628-3.ABSTRACTThe homologous to the E6-AP carboxyl terminus (HECT)-type E3 ubiquitin ligases are the selective executers in the protein ubiquitination, playing a vital role in modulation of the protein function and stability. Evidence shows the regulatory role of HECT-type E3 ligases in various steps of the autophagic process. Autophagy is an intracellular digestive and recycling process that controls the cellular hemostasis. Defective autophagy is involved in tumorigenesis and has been detected in various types of cancer cells. A growing body of findings indicates t...
Source: Molecular Medicine - March 15, 2023 Category: Molecular Biology Authors: Rui Zhang Shaoqing Shi Source Type: research
Telomerase inhibition in malignant gliomas. A systematic review
Expert Rev Mol Med. 2023 Mar 15:1-25. doi: 10.1017/erm.2023.6. Online ahead of print.NO ABSTRACTPMID:36919343 | DOI:10.1017/erm.2023.6 (Source: Molecular Medicine)
Source: Molecular Medicine - March 15, 2023 Category: Molecular Biology Authors: Quintino Giorgio D'Alessandris Marco Battistelli Giovanni Pennisi Martina Offi Maurizio Martini Tonia Cenci Maria Laura Falchetti Liverana Lauretti Alessandro Olivi Roberto Pallini Nicola Montano Source Type: research
Identification of a CEACAM5 targeted nanobody for positron emission tomography imaging and near-infrared fluorescence imaging of colorectal cancer
CONCLUSION: Collectively, we presented the identification and in vivo validation of a CEACAM5-targeted nanobody and a fused nanobody with an ABD, which enabled to the non-invasive visualization of malignancy of CRC using PET imaging and NIR imaging in subcutaneous models as well as LNM models.PMID:36914753 | DOI:10.1007/s00259-023-06183-7 (Source: Molecular Medicine)
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Yitai Xiao Chaoming Mei Duo Xu Fan Yang Meilin Yang Lei Bi Junjie Mao Pengfei Pang Dan Li Source Type: research
Levosimendan inhibits disulfide tau oligomerization and ameliorates tau pathology in Tau < sup > P301L < /sup > -BiFC mice
Exp Mol Med. 2023 Mar 13. doi: 10.1038/s12276-023-00959-5. Online ahead of print.ABSTRACTTau oligomers play critical roles in tau pathology and are responsible for neuronal cell death and transmitting the disease in the brain. Accordingly, preventing tau oligomerization has become an important therapeutic strategy to treat tauopathies, including Alzheimer's disease. However, progress has been slow because detecting tau oligomers in the cellular context is difficult. Working toward tau-targeted drug discovery, our group has developed a tau-BiFC platform to monitor and quantify tau oligomerization. By using the tau-BiFC plat...
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Sungsu Lim Seulgi Shin Yoonsik Sung Ha Eun Lee Kyu Hyeon Kim Ji Yeon Song Gwan-Ho Lee Hira Aziz Nataliia Lukianenko Dong Min Kang Nicolette Boesen Hyeanjeong Jeong Aizhan Abdildinova Junghee Lee Byung-Yong Yu Sang Min Lim Jun-Seok Lee Hoon Ryu Ae Nim Pae Source Type: research
Single-cell sequencing reveals that endothelial cells, EndMT cells and mural cells contribute to the pathogenesis of cavernous malformations
Exp Mol Med. 2023 Mar 13. doi: 10.1038/s12276-023-00962-w. Online ahead of print.ABSTRACTCavernous malformations (CMs) invading the central nervous system occur in ~0.16-0.4% of the general population, often resulting in hemorrhages and focal neurological deficits. Further understanding of disease mechanisms and therapeutic strategies requires a deeper knowledge of CMs in humans. Herein, we performed single-cell RNA sequencing (scRNA-seq) analysis on unselected viable cells from twelve human CM samples and three control samples. A total of 112,670 high-quality cells were clustered into 11 major cell types, which shared a n...
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Jian Ren Xiao Xiao Ruofei Li Cheng Lv Yu Zhang Leiming Wang Tao Hong Hongqi Zhang Yibo Wang Source Type: research
Correction: Requirement for hippocampal CA3 NMDA receptors in artificial association of memory events stored in CA3 cell ensembles
Mol Brain. 2023 Mar 13;16(1):29. doi: 10.1186/s13041-023-01016-y.NO ABSTRACTPMID:36915114 | DOI:10.1186/s13041-023-01016-y (Source: Molecular Medicine)
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Masanori Nomoto Noriaki Ohkawa Kaoru Inokuchi Naoya Oishi Source Type: research
Influence of cyclin D1 splicing variants expression on breast cancer chemoresistance via CDK4/CyclinD1-pRB-E2F1 pathway
J Cell Mol Med. 2023 Mar 13. doi: 10.1111/jcmm.17716. Online ahead of print.ABSTRACTCyclin D1 (CCND1), a mediator of cell cycle control, has a G870A polymorphism which results in the formation of two splicing variants: full-length CCND1 (CCND1a) and C-terminally truncated CCND1 species (CCND1b). However, the role of CCND1a and CCND1b variants in cancer chemoresistance remains unknown. Therefore, this study aimed to explore the molecular mechanism of alternative splicing of CCND1 in breast cancer (BC) chemoresistance. To address the contribution of G870A polymorphism to the production of CCND1 variants in BC chemoresistance...
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Jing Wang Jiaxin Zhang Qinglong Ma Shasha Zhang Fengdie Ma Wei Su Taotao Zhang Xiaodong Xie Cuixia Di Source Type: research
4-octyl itaconate improves the viability of D66H cells by regulating the KEAP1-NRF2-GCLC/HO-1 pathway
In conclusion, OI could improve the viability of HaCaT and D66H cells via regulating the KEAP1-NRF2-GCLC/HO-1 pathway, which provided a wide spectrum of potential targets for effective therapeutic treatments of Vohwinkel syndrome in the clinic.PMID:36916028 | DOI:10.1111/jcmm.17708 (Source: Molecular Medicine)
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Yanrui Chen Zhenying Wang Yali Song Nan Chen Jing Guo Wenmin Liu Keying Guo Xia Ling Li Zhang Source Type: research
Effects of calreticulin mutations on cell transformation and immunity
J Cell Mol Med. 2023 Mar 13. doi: 10.1111/jcmm.17713. Online ahead of print.ABSTRACTMyeloproliferative neoplasms (MPNs) are cancers involving dysregulated production and function of myeloid lineage hematopoietic cells. Among MPNs, Essential thrombocythemia (ET), Polycythemia Vera (PV) and Myelofibrosis (MF), are driven by mutations that activate the JAK-STAT signalling pathway. Somatic mutations of calreticulin (CRT), an endoplasmic reticulum (ER)-localized lectin chaperone, are driver mutations in approximately 25% of ET and 35% of MF patients. The MPN-linked mutant CRT proteins have novel frameshifted carboxy-domain sequ...
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Harini Desikan Amanpreet Kaur Irina D Pogozheva Malini Raghavan Source Type: research
Bi-allelic mutation in SEC16B alters collagen trafficking and increases ER stress
EMBO Mol Med. 2023 Mar 14:e16834. doi: 10.15252/emmm.202216834. Online ahead of print.ABSTRACTOsteogenesis imperfecta (OI) is a genetically and clinically heterogeneous disorder characterized by bone fragility and reduced bone mass generally caused by defects in type I collagen structure or defects in proteins interacting with collagen processing. We identified a homozygous missense mutation in SEC16B in a child with vertebral fractures, leg bowing, short stature, muscular hypotonia, and bone densitometric and histomorphometric features in keeping with OI with distinct ultrastructural features. In line with the putative fu...
Source: Molecular Medicine - March 14, 2023 Category: Molecular Biology Authors: Ahmed El-Gazzar Barbara Voraberger Frank Rauch Mario Mairhofer Katy Schmidt Brecht Guillemyn Goran Mitulovi ć Veronika Reiterer Margot Haun Michaela M Mayr Johannes A Mayr Susanne Kimeswenger Oliver Drews Vrinda Saraff Nick Shaw Nadja Fratzl-Zelman Sofie Source Type: research
