Tissue Transglutaminase 2 has higher affinity for relaxed than for stretched fibronectin fibers
Matrix Biol. 2023 Dec 20:S0945-053X(23)00129-4. doi: 10.1016/j.matbio.2023.12.006. Online ahead of print.ABSTRACTTissue transglutaminase 2 (TG2) plays a vital role in stabilizing extracellular matrix (ECM) proteins through enzymatic crosslinking during tissue growth, repair, and inflammation. TG2 also binds non-covalently to fibronectin (FN), an essential component of the ECM, facilitating cell adhesion, migration, proliferation, and survival. However, the interaction between TG2 and fibrillar FN remains poorly understood, as most studies have focused on soluble or surface-adsorbed FN or FN fragments, which differ in their...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kateryna Selcuk Alexander Leitner Lukas Braun Fanny Le Blanc Paulina Pacak Simon Pot Viola Vogel Source Type: research
A role for decorin in improving motor deficits after traumatic brain injury
In this study, we sought to delineate the dynamics of glycosaminoglycan alterations after TBI and discover the precise biologic processes responsible for observed glycosaminoglycan changes after injury. We performed state-of-the art mass spectrometry on brain tissues isolated from mice after TBI or craniotomy-alone. We observed dynamic changes in glycosaminoglycans at Day 1 and 7 post-TBI, with heparan sulfate, chondroitin sulfate, and hyaluronan remaining significantly increased after a week vis-à-vis craniotomy-alone tissues. We did not observe appreciable changes in circulating glycosaminoglycans in mice after experime...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kaori Oshima Noah Siddiqui James E Orfila Danelle Carter Justin Laing Xiaorui Han Igor Zakharevich Renato V Iozzo Arsen Ghasabayan Hunter Moore Fuming Zhang Robert J Linhardt Ernest E Moore Nidia Quillinan Eric P Schmidt Paco S Herson Joseph A Hippensteel Source Type: research
Tissue Transglutaminase 2 has higher affinity for relaxed than for stretched fibronectin fibers
Matrix Biol. 2023 Dec 20:S0945-053X(23)00129-4. doi: 10.1016/j.matbio.2023.12.006. Online ahead of print.ABSTRACTTissue transglutaminase 2 (TG2) plays a vital role in stabilizing extracellular matrix (ECM) proteins through enzymatic crosslinking during tissue growth, repair, and inflammation. TG2 also binds non-covalently to fibronectin (FN), an essential component of the ECM, facilitating cell adhesion, migration, proliferation, and survival. However, the interaction between TG2 and fibrillar FN remains poorly understood, as most studies have focused on soluble or surface-adsorbed FN or FN fragments, which differ in their...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kateryna Selcuk Alexander Leitner Lukas Braun Fanny Le Blanc Paulina Pacak Simon Pot Viola Vogel Source Type: research
A role for decorin in improving motor deficits after traumatic brain injury
In this study, we sought to delineate the dynamics of glycosaminoglycan alterations after TBI and discover the precise biologic processes responsible for observed glycosaminoglycan changes after injury. We performed state-of-the art mass spectrometry on brain tissues isolated from mice after TBI or craniotomy-alone. We observed dynamic changes in glycosaminoglycans at Day 1 and 7 post-TBI, with heparan sulfate, chondroitin sulfate, and hyaluronan remaining significantly increased after a week vis-à-vis craniotomy-alone tissues. We did not observe appreciable changes in circulating glycosaminoglycans in mice after experime...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kaori Oshima Noah Siddiqui James E Orfila Danelle Carter Justin Laing Xiaorui Han Igor Zakharevich Renato V Iozzo Arsen Ghasabayan Hunter Moore Fuming Zhang Robert J Linhardt Ernest E Moore Nidia Quillinan Eric P Schmidt Paco S Herson Joseph A Hippensteel Source Type: research
Tissue Transglutaminase 2 has higher affinity for relaxed than for stretched fibronectin fibers
Matrix Biol. 2023 Dec 20:S0945-053X(23)00129-4. doi: 10.1016/j.matbio.2023.12.006. Online ahead of print.ABSTRACTTissue transglutaminase 2 (TG2) plays a vital role in stabilizing extracellular matrix (ECM) proteins through enzymatic crosslinking during tissue growth, repair, and inflammation. TG2 also binds non-covalently to fibronectin (FN), an essential component of the ECM, facilitating cell adhesion, migration, proliferation, and survival. However, the interaction between TG2 and fibrillar FN remains poorly understood, as most studies have focused on soluble or surface-adsorbed FN or FN fragments, which differ in their...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kateryna Selcuk Alexander Leitner Lukas Braun Fanny Le Blanc Paulina Pacak Simon Pot Viola Vogel Source Type: research
Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing
Matrix Biol. 2023 Dec 13:S0945-053X(23)00127-0. doi: 10.1016/j.matbio.2023.12.004. Online ahead of print.ABSTRACTTendon injuries are a major clinical problem, with poor patient outcomes caused by abundant scar tissue deposition during healing. Myofibroblasts play a critical role in the initial restoration of structural integrity after injury. However, persistent myofibroblast activity drives the transition to fibrotic scar tissue formation. As such, disrupting myofibroblast persistence is a key therapeutic target. While myofibroblasts are typically defined by the presence of αSMA+ stress fibers, αSMA is expressed in othe...
Source: Matrix Biology - December 15, 2023 Category: Molecular Biology Authors: Jessica E Ackerman Samantha N Muscat Emmanuela Adjei-Sowah Antonion Korcari Anne E C Nichols Mark R Buckley Alayna E Loiselle Source Type: research
Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing
Matrix Biol. 2023 Dec 13:S0945-053X(23)00127-0. doi: 10.1016/j.matbio.2023.12.004. Online ahead of print.ABSTRACTTendon injuries are a major clinical problem, with poor patient outcomes caused by abundant scar tissue deposition during healing. Myofibroblasts play a critical role in the initial restoration of structural integrity after injury. However, persistent myofibroblast activity drives the transition to fibrotic scar tissue formation. As such, disrupting myofibroblast persistence is a key therapeutic target. While myofibroblasts are typically defined by the presence of αSMA+ stress fibers, αSMA is expressed in othe...
Source: Matrix Biology - December 15, 2023 Category: Molecular Biology Authors: Jessica E Ackerman Samantha N Muscat Emmanuela Adjei-Sowah Antonion Korcari Anne E C Nichols Mark R Buckley Alayna E Loiselle Source Type: research
Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing
Matrix Biol. 2023 Dec 13:S0945-053X(23)00127-0. doi: 10.1016/j.matbio.2023.12.004. Online ahead of print.ABSTRACTTendon injuries are a major clinical problem, with poor patient outcomes caused by abundant scar tissue deposition during healing. Myofibroblasts play a critical role in the initial restoration of structural integrity after injury. However, persistent myofibroblast activity drives the transition to fibrotic scar tissue formation. As such, disrupting myofibroblast persistence is a key therapeutic target. While myofibroblasts are typically defined by the presence of αSMA+ stress fibers, αSMA is expressed in othe...
Source: Matrix Biology - December 15, 2023 Category: Molecular Biology Authors: Jessica E Ackerman Samantha N Muscat Emmanuela Adjei-Sowah Antonion Korcari Anne E C Nichols Mark R Buckley Alayna E Loiselle Source Type: research
Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing
Matrix Biol. 2023 Dec 13:S0945-053X(23)00127-0. doi: 10.1016/j.matbio.2023.12.004. Online ahead of print.ABSTRACTTendon injuries are a major clinical problem, with poor patient outcomes caused by abundant scar tissue deposition during healing. Myofibroblasts play a critical role in the initial restoration of structural integrity after injury. However, persistent myofibroblast activity drives the transition to fibrotic scar tissue formation. As such, disrupting myofibroblast persistence is a key therapeutic target. While myofibroblasts are typically defined by the presence of αSMA+ stress fibers, αSMA is expressed in othe...
Source: Matrix Biology - December 15, 2023 Category: Molecular Biology Authors: Jessica E Ackerman Samantha N Muscat Emmanuela Adjei-Sowah Antonion Korcari Anne E C Nichols Mark R Buckley Alayna E Loiselle Source Type: research
Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing
Matrix Biol. 2023 Dec 13:S0945-053X(23)00127-0. doi: 10.1016/j.matbio.2023.12.004. Online ahead of print.ABSTRACTTendon injuries are a major clinical problem, with poor patient outcomes caused by abundant scar tissue deposition during healing. Myofibroblasts play a critical role in the initial restoration of structural integrity after injury. However, persistent myofibroblast activity drives the transition to fibrotic scar tissue formation. As such, disrupting myofibroblast persistence is a key therapeutic target. While myofibroblasts are typically defined by the presence of αSMA+ stress fibers, αSMA is expressed in othe...
Source: Matrix Biology - December 15, 2023 Category: Molecular Biology Authors: Jessica E Ackerman Samantha N Muscat Emmanuela Adjei-Sowah Antonion Korcari Anne E C Nichols Mark R Buckley Alayna E Loiselle Source Type: research
Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing
Matrix Biol. 2023 Dec 13:S0945-053X(23)00127-0. doi: 10.1016/j.matbio.2023.12.004. Online ahead of print.ABSTRACTTendon injuries are a major clinical problem, with poor patient outcomes caused by abundant scar tissue deposition during healing. Myofibroblasts play a critical role in the initial restoration of structural integrity after injury. However, persistent myofibroblast activity drives the transition to fibrotic scar tissue formation. As such, disrupting myofibroblast persistence is a key therapeutic target. While myofibroblasts are typically defined by the presence of αSMA+ stress fibers, αSMA is expressed in othe...
Source: Matrix Biology - December 15, 2023 Category: Molecular Biology Authors: Jessica E Ackerman Samantha N Muscat Emmanuela Adjei-Sowah Antonion Korcari Anne E C Nichols Mark R Buckley Alayna E Loiselle Source Type: research
Novel insights into the role of Discoidin Domain Receptor 2 (DDR2) in cancer progression: a new avenue of therapeutic intervention
Matrix Biol. 2023 Dec 9:S0945-053X(23)00126-9. doi: 10.1016/j.matbio.2023.12.003. Online ahead of print.ABSTRACTDiscoidin domain receptors (DDRs), including DDR1 and DDR2, are a unique class of receptor tyrosine kinases (RTKs) activated by collagens at the cell-matrix boundary interface. The peculiar mode of activation makes DDRs key cellular sensors of microenvironmental changes, with a critical role in all physiological and pathological processes governed by collagen remodeling. DDRs are widely expressed in fetal and adult tissues, and experimental and clinical evidence has shown that their expression is deregulated in c...
Source: Matrix Biology - December 11, 2023 Category: Molecular Biology Authors: Paola Trono Flavia Ottavi Laura Rosan ò Source Type: research
Collagen prolyl 4-hydroxylase isoenzymes I and II have sequence specificity towards different X-Pro-Gly triplets
In conclusion, this study provides a molecular level explanation for the need of multiple C-P4H isoenzymes to generate collagen molecules capable to assemble into intact extracellular matrix structures.PMID:38081527 | DOI:10.1016/j.matbio.2023.12.001 (Source: Matrix Biology)
Source: Matrix Biology - December 11, 2023 Category: Molecular Biology Authors: Antti M Salo Pekka Rappu M Kristian Koski Emma Karjalainen Valerio Izzi Kati Drushinin Ilkka Miinalainen Jarmo K äpylä Jyrki Heino Johanna Myllyharju Source Type: research
Novel insights into the role of Discoidin Domain Receptor 2 (DDR2) in cancer progression: a new avenue of therapeutic intervention
Matrix Biol. 2023 Dec 9:S0945-053X(23)00126-9. doi: 10.1016/j.matbio.2023.12.003. Online ahead of print.ABSTRACTDiscoidin domain receptors (DDRs), including DDR1 and DDR2, are a unique class of receptor tyrosine kinases (RTKs) activated by collagens at the cell-matrix boundary interface. The peculiar mode of activation makes DDRs key cellular sensors of microenvironmental changes, with a critical role in all physiological and pathological processes governed by collagen remodeling. DDRs are widely expressed in fetal and adult tissues, and experimental and clinical evidence has shown that their expression is deregulated in c...
Source: Matrix Biology - December 11, 2023 Category: Molecular Biology Authors: Paola Trono Flavia Ottavi Laura Rosan ò Source Type: research
Collagen prolyl 4-hydroxylase isoenzymes I and II have sequence specificity towards different X-Pro-Gly triplets
In conclusion, this study provides a molecular level explanation for the need of multiple C-P4H isoenzymes to generate collagen molecules capable to assemble into intact extracellular matrix structures.PMID:38081527 | DOI:10.1016/j.matbio.2023.12.001 (Source: Matrix Biology)
Source: Matrix Biology - December 11, 2023 Category: Molecular Biology Authors: Antti M Salo Pekka Rappu M Kristian Koski Emma Karjalainen Valerio Izzi Kati Drushinin Ilkka Miinalainen Jarmo K äpylä Jyrki Heino Johanna Myllyharju Source Type: research
