Keratinocyte integrin α3β1 induces expression of the macrophage stimulating factor, CSF-1, through a YAP/TEAD-dependent mechanism
Matrix Biol. 2024 Feb 8:S0945-053X(24)00022-2. doi: 10.1016/j.matbio.2024.02.003. Online ahead of print.ABSTRACTThe development of wound therapy targeting integrins is hampered by inadequate understanding of integrin function in cutaneous wound healing and the wound microenvironment. Following cutaneous injury, keratinocytes migrate to restore the skin barrier, and macrophages aid in debris clearance. Thus, both keratinocytes and macrophages are critical to the coordination of tissue repair. Keratinocyte integrins have been shown to participate in this coordinated effort by regulating secreted factors, some of which crosst...
Source: Matrix Biology - February 10, 2024 Category: Molecular Biology Authors: Whitney M Longmate Emily Norton Giesse Albeche Duarte Lei Wu Mathieu R DiPersio John M Lamar C Michael DiPersio Source Type: research
Dysfunctional Latent Transforming Growth Factor β Activation After Corneal Injury in a Classical Ehlers-Danlos Model
Matrix Biol. 2024 Feb 8:S0945-053X(24)00023-4. doi: 10.1016/j.matbio.2024.02.004. Online ahead of print.ABSTRACTPatients with classical Ehlers Danlos syndrome (cEDS) suffer impaired wound healing and from scars formed after injuries that are atrophic and difficult to close surgically. Haploinsufficiency in COL5A1 creates systemic morphological and functional alterations in the entire body. We investigated mechanisms that impair wound healing from corneal lacerations (full thickness injuries) in a mouse model of cEDS (Col5a1+/-). We found that collagen V reexpression in this model is upregulated during corneal tissue repair...
Source: Matrix Biology - February 10, 2024 Category: Molecular Biology Authors: Mei Sun Ana Carolina Acosta Victoria Emerick Sheila Adams Marcel Y Avila Curtis E Margo Edgar M Espana Source Type: research
Keratinocyte integrin α3β1 induces expression of the macrophage stimulating factor, CSF-1, through a YAP/TEAD-dependent mechanism
Matrix Biol. 2024 Feb 8:S0945-053X(24)00022-2. doi: 10.1016/j.matbio.2024.02.003. Online ahead of print.ABSTRACTThe development of wound therapy targeting integrins is hampered by inadequate understanding of integrin function in cutaneous wound healing and the wound microenvironment. Following cutaneous injury, keratinocytes migrate to restore the skin barrier, and macrophages aid in debris clearance. Thus, both keratinocytes and macrophages are critical to the coordination of tissue repair. Keratinocyte integrins have been shown to participate in this coordinated effort by regulating secreted factors, some of which crosst...
Source: Matrix Biology - February 10, 2024 Category: Molecular Biology Authors: Whitney M Longmate Emily Norton Giesse Albeche Duarte Lei Wu Mathieu R DiPersio John M Lamar C Michael DiPersio Source Type: research
Dysfunctional Latent Transforming Growth Factor β Activation After Corneal Injury in a Classical Ehlers-Danlos Model
Matrix Biol. 2024 Feb 8:S0945-053X(24)00023-4. doi: 10.1016/j.matbio.2024.02.004. Online ahead of print.ABSTRACTPatients with classical Ehlers Danlos syndrome (cEDS) suffer impaired wound healing and from scars formed after injuries that are atrophic and difficult to close surgically. Haploinsufficiency in COL5A1 creates systemic morphological and functional alterations in the entire body. We investigated mechanisms that impair wound healing from corneal lacerations (full thickness injuries) in a mouse model of cEDS (Col5a1+/-). We found that collagen V reexpression in this model is upregulated during corneal tissue repair...
Source: Matrix Biology - February 10, 2024 Category: Molecular Biology Authors: Mei Sun Ana Carolina Acosta Victoria Emerick Sheila Adams Marcel Y Avila Curtis E Margo Edgar M Espana Source Type: research
Keratinocyte integrin α3β1 induces expression of the macrophage stimulating factor, CSF-1, through a YAP/TEAD-dependent mechanism
Matrix Biol. 2024 Feb 8:S0945-053X(24)00022-2. doi: 10.1016/j.matbio.2024.02.003. Online ahead of print.ABSTRACTThe development of wound therapy targeting integrins is hampered by inadequate understanding of integrin function in cutaneous wound healing and the wound microenvironment. Following cutaneous injury, keratinocytes migrate to restore the skin barrier, and macrophages aid in debris clearance. Thus, both keratinocytes and macrophages are critical to the coordination of tissue repair. Keratinocyte integrins have been shown to participate in this coordinated effort by regulating secreted factors, some of which crosst...
Source: Matrix Biology - February 10, 2024 Category: Molecular Biology Authors: Whitney M Longmate Emily Norton Giesse Albeche Duarte Lei Wu Mathieu R DiPersio John M Lamar C Michael DiPersio Source Type: research
Dysfunctional Latent Transforming Growth Factor β Activation After Corneal Injury in a Classical Ehlers-Danlos Model
Matrix Biol. 2024 Feb 8:S0945-053X(24)00023-4. doi: 10.1016/j.matbio.2024.02.004. Online ahead of print.ABSTRACTPatients with classical Ehlers Danlos syndrome (cEDS) suffer impaired wound healing and from scars formed after injuries that are atrophic and difficult to close surgically. Haploinsufficiency in COL5A1 creates systemic morphological and functional alterations in the entire body. We investigated mechanisms that impair wound healing from corneal lacerations (full thickness injuries) in a mouse model of cEDS (Col5a1+/-). We found that collagen V reexpression in this model is upregulated during corneal tissue repair...
Source: Matrix Biology - February 10, 2024 Category: Molecular Biology Authors: Mei Sun Ana Carolina Acosta Victoria Emerick Sheila Adams Marcel Y Avila Curtis E Margo Edgar M Espana Source Type: research
Keratinocyte integrin α3β1 induces expression of the macrophage stimulating factor, CSF-1, through a YAP/TEAD-dependent mechanism
Matrix Biol. 2024 Feb 8:S0945-053X(24)00022-2. doi: 10.1016/j.matbio.2024.02.003. Online ahead of print.ABSTRACTThe development of wound therapy targeting integrins is hampered by inadequate understanding of integrin function in cutaneous wound healing and the wound microenvironment. Following cutaneous injury, keratinocytes migrate to restore the skin barrier, and macrophages aid in debris clearance. Thus, both keratinocytes and macrophages are critical to the coordination of tissue repair. Keratinocyte integrins have been shown to participate in this coordinated effort by regulating secreted factors, some of which crosst...
Source: Matrix Biology - February 10, 2024 Category: Molecular Biology Authors: Whitney M Longmate Emily Norton Giesse Albeche Duarte Lei Wu Mathieu R DiPersio John M Lamar C Michael DiPersio Source Type: research
Kidney resident macrophages have distinct subsets and multifunctional roles
CONCLUSIONS: We identified CD81 and CX3CR1 positive kidney macrophage subsets with distinct dependence for monocyte replenishment. Multiomic analysis demonstrated that these cells have diverse functions that underscore the importance of macrophages in kidney health and disease.PMID:38331051 | DOI:10.1016/j.matbio.2024.02.002 (Source: Matrix Biology)
Source: Matrix Biology - February 8, 2024 Category: Molecular Biology Authors: Christine Chew Oliver J Brand Tomohiko Yamamura Craig Lawless Mychel Raony Paiva Teixeira Morais Leo Zeef I-Hsuan Lin Gareth Howell Sylvia Lui Franziska Lausecker Christopher Jagger Tovah N Shaw Siddharth Krishnan Flora A McClure Hayley Bridgeman Kelly We Source Type: research
Novel muscle-derived extracellular matrix hydrogel promotes angiogenesis and neurogenesis in volumetric muscle loss
Matrix Biol. 2024 Feb 5:S0945-053X(24)00020-9. doi: 10.1016/j.matbio.2024.02.001. Online ahead of print.ABSTRACTVolumetric muscle loss (VML) represents a clinical challenge due to the limited regenerative capacity of skeletal muscle. Most often, it results in scar tissue formation and loss of function, which cannot be prevented by current therapies. Decellularized extracellular matrix (DEM) has emerged as a native biomaterial for the enhancement of tissue repair. Here, we report the generation and characterization of hydrogels derived from DEM prepared from WT or thrombospondin (TSP)-2 null muscle tissue. TSP2-null hydroge...
Source: Matrix Biology - February 7, 2024 Category: Molecular Biology Authors: Zhuoyue Chen Yaqing Huang Hao Xing Tiffany Tseng Hailey Edelman Rachel Perry Themis R Kyriakides Source Type: research
Thrombospondin-1 promotes mechanical stress-mediated ligamentum flavum hypertrophy through the TGF β1/Smad3 signaling pathway
Matrix Biol. 2024 Jan 26:S0945-053X(24)00013-1. doi: 10.1016/j.matbio.2024.01.005. Online ahead of print.ABSTRACTLumbar spinal canal stenosis is primarily caused by ligamentum flavum hypertrophy (LFH), which is a significant pathological factor. Nevertheless, the precise molecular basis for the development of LFH remains uncertain. The current investigation observed a notable increase in thrombospondin-1 (THBS1) expression in LFH through proteomics analysis and single-cell RNA-sequencing analysis of clinical ligamentum flavum specimens. In laboratory experiments, it was demonstrated that THBS1 triggered the activation of S...
Source: Matrix Biology - January 28, 2024 Category: Molecular Biology Authors: Run Zhao Jiale Dong Chunlei Liu Mingheng Li Ruiqian Tan Chengshuo Fei Yanlin Chen Xinxing Yang Jiawei Shi Jiajia Xu Liang Wang Peng Li Zhongmin Zhang Source Type: research
Inter-alpha-trypsin inhibitor (i(i) and hyaluronan modifications enhance the innate immune response to influenza virus in the lung
In conclusion, this work shows increased IαI and HA accumulation and the formation of a HC•HA matrix in mouse lungs post-IAV infection. The formation of HA and HC•HA matrices could potentially create specific microenvironments in the lungs for immune cell recruitment and activation during IAV infection.PMID:38232913 | DOI:10.1016/j.matbio.2024.01.004 (Source: Matrix Biology)
Source: Matrix Biology - January 17, 2024 Category: Molecular Biology Authors: Fengying Tang Stephen R Reeves Jourdan E Brune Mary Y Chang Christina K Chan Peter Waldron Sheona P Drummond Caroline M Milner Kimberly M Alonge Stavros Garantziotis Anthony J Day William A Altemeier Charles W Frevert Source Type: research
Three-dimensional co-culturing of stem cell-derived cardiomyocytes and cardiac fibroblasts reveals a role for both cell types in Marfan-related cardiomyopathy
Matrix Biol. 2024 Jan 13:S0945-053X(24)00003-9. doi: 10.1016/j.matbio.2024.01.003. Online ahead of print.ABSTRACTPathogenic variants in the FBN1 gene, which encodes the extracellular matrix protein fibrillin-1, cause Marfan syndrome (MFS), which affects multiple organ systems, including the cardiovascular system. Myocardial dysfunction has been observed in a subset of patients with MFS and in several MFS mouse models. However, there is limited understanding of the intrinsic consequences of FBN1 variants on cardiomyocytes (CMs). To elucidate the CM-specific contribution in Marfan's cardiomyopathy, cardiosphere cultures of C...
Source: Matrix Biology - January 15, 2024 Category: Molecular Biology Authors: Jeffrey Aalders Laurens L éger Louis Van der Meeren Sanjay Sinha Andre G Skirtach Julie De Backer Jolanda van Hengel Source Type: research
S100A4 a classical DAMP as a therapeutic target in fibrosis
Matrix Biol. 2024 Jan 12:S0945-053X(24)00002-7. doi: 10.1016/j.matbio.2024.01.002. Online ahead of print.ABSTRACTFibrosis regardless of aetiology is characterised by persistently activated myofibroblasts that are contractile and secrete excessive amounts of extracellular matrix molecules that leads to loss of organ function. Damage-Associated Molecular Patterns (DAMPs) are endogenous host-derived molecules that are released from cells dying or under stress that can be triggered by a variety of insults, either chemical or physical, leading to an inflammatory response. Among these DAMPs is S100A4, part of the S100 family of ...
Source: Matrix Biology - January 14, 2024 Category: Molecular Biology Authors: Steven O'Reilly Source Type: research
Dynamic changes in mitral valve extracellular matrix, tissue mechanics and function in a mouse model of Marfan syndrome
CONCLUSIONS: Altogether, our data indicate dynamic regulation of mitral valve structure, tissue mechanics, and function that reflect changes in ECM composition, organization, and gene expression in progressive MVD. Notably, increased collagen fiber organization and orientation, potentially dependent on increased matrifibrocyte cell activity, is apparent with altered mitral valve mechanics and function in aging MFS mice.PMID:38185344 | DOI:10.1016/j.matbio.2024.01.001 (Source: Matrix Biology)
Source: Matrix Biology - January 7, 2024 Category: Molecular Biology Authors: Brittany A Gonzalez Samuel W Harmeyer Taejeong Song Sakthivel Sadayappan Katherine E Yutzey Source Type: research
Dynamic changes in mitral valve extracellular matrix, tissue mechanics and function in a mouse model of Marfan syndrome
CONCLUSIONS: Altogether, our data indicate dynamic regulation of mitral valve structure, tissue mechanics, and function that reflect changes in ECM composition, organization, and gene expression in progressive MVD. Notably, increased collagen fiber organization and orientation, potentially dependent on increased matrifibrocyte cell activity, is apparent with altered mitral valve mechanics and function in aging MFS mice.PMID:38185344 | DOI:10.1016/j.matbio.2024.01.001 (Source: Matrix Biology)
Source: Matrix Biology - January 7, 2024 Category: Molecular Biology Authors: Brittany A Gonzalez Samuel W Harmeyer Taejeong Song Sakthivel Sadayappan Katherine E Yutzey Source Type: research
