Dynamic changes in mitral valve extracellular matrix, tissue mechanics and function in a mouse model of Marfan syndrome
CONCLUSIONS: Altogether, our data indicate dynamic regulation of mitral valve structure, tissue mechanics, and function that reflect changes in ECM composition, organization, and gene expression in progressive MVD. Notably, increased collagen fiber organization and orientation, potentially dependent on increased matrifibrocyte cell activity, is apparent with altered mitral valve mechanics and function in aging MFS mice.PMID:38185344 | DOI:10.1016/j.matbio.2024.01.001 (Source: Matrix Biology)
Source: Matrix Biology - January 7, 2024 Category: Molecular Biology Authors: Brittany A Gonzalez Samuel W Harmeyer Taejeong Song Sakthivel Sadayappan Katherine E Yutzey Source Type: research
Dynamic changes in mitral valve extracellular matrix, tissue mechanics and function in a mouse model of Marfan syndrome
CONCLUSIONS: Altogether, our data indicate dynamic regulation of mitral valve structure, tissue mechanics, and function that reflect changes in ECM composition, organization, and gene expression in progressive MVD. Notably, increased collagen fiber organization and orientation, potentially dependent on increased matrifibrocyte cell activity, is apparent with altered mitral valve mechanics and function in aging MFS mice.PMID:38185344 | DOI:10.1016/j.matbio.2024.01.001 (Source: Matrix Biology)
Source: Matrix Biology - January 7, 2024 Category: Molecular Biology Authors: Brittany A Gonzalez Samuel W Harmeyer Taejeong Song Sakthivel Sadayappan Katherine E Yutzey Source Type: research
Dynamic changes in mitral valve extracellular matrix, tissue mechanics and function in a mouse model of Marfan syndrome
CONCLUSIONS: Altogether, our data indicate dynamic regulation of mitral valve structure, tissue mechanics, and function that reflect changes in ECM composition, organization, and gene expression in progressive MVD. Notably, increased collagen fiber organization and orientation, potentially dependent on increased matrifibrocyte cell activity, is apparent with altered mitral valve mechanics and function in aging MFS mice.PMID:38185344 | DOI:10.1016/j.matbio.2024.01.001 (Source: Matrix Biology)
Source: Matrix Biology - January 7, 2024 Category: Molecular Biology Authors: Brittany A Gonzalez Samuel W Harmeyer Taejeong Song Sakthivel Sadayappan Katherine E Yutzey Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
Inhibiting JAK1, not NF- κB, reverses the effect of pro-inflammatory cytokines on engineered human ligament function
Matrix Biol. 2023 Dec 25:S0945-053X(23)00130-0. doi: 10.1016/j.matbio.2023.12.007. Online ahead of print.ABSTRACTThe role of inflammation in chronic tendon/ligament injury is hotly debated. There is less debate about inflammation following acute injury. To better understand the effect of acute inflammation, in this study we developed a multi-cytokine model of inflammatory tendinitis. The combined treatment with TNF-α, IL-1β, and IL-6, at dosages well below what are routinely used in vitro, decreased the mechanical properties and collagen content of engineered human ligaments. Treatment with this cytokine mixture resulted...
Source: Matrix Biology - December 27, 2023 Category: Molecular Biology Authors: Alec M Avey Florence Devos Albany G Roberts El Sayed El Essawy Keith Baar Source Type: research
A role for decorin in improving motor deficits after traumatic brain injury
In this study, we sought to delineate the dynamics of glycosaminoglycan alterations after TBI and discover the precise biologic processes responsible for observed glycosaminoglycan changes after injury. We performed state-of-the art mass spectrometry on brain tissues isolated from mice after TBI or craniotomy-alone. We observed dynamic changes in glycosaminoglycans at Day 1 and 7 post-TBI, with heparan sulfate, chondroitin sulfate, and hyaluronan remaining significantly increased after a week vis-à-vis craniotomy-alone tissues. We did not observe appreciable changes in circulating glycosaminoglycans in mice after experime...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kaori Oshima Noah Siddiqui James E Orfila Danelle Carter Justin Laing Xiaorui Han Igor Zakharevich Renato V Iozzo Arsen Ghasabayan Hunter Moore Fuming Zhang Robert J Linhardt Ernest E Moore Nidia Quillinan Eric P Schmidt Paco S Herson Joseph A Hippensteel Source Type: research
Tissue Transglutaminase 2 has higher affinity for relaxed than for stretched fibronectin fibers
Matrix Biol. 2023 Dec 20:S0945-053X(23)00129-4. doi: 10.1016/j.matbio.2023.12.006. Online ahead of print.ABSTRACTTissue transglutaminase 2 (TG2) plays a vital role in stabilizing extracellular matrix (ECM) proteins through enzymatic crosslinking during tissue growth, repair, and inflammation. TG2 also binds non-covalently to fibronectin (FN), an essential component of the ECM, facilitating cell adhesion, migration, proliferation, and survival. However, the interaction between TG2 and fibrillar FN remains poorly understood, as most studies have focused on soluble or surface-adsorbed FN or FN fragments, which differ in their...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kateryna Selcuk Alexander Leitner Lukas Braun Fanny Le Blanc Paulina Pacak Simon Pot Viola Vogel Source Type: research
A role for decorin in improving motor deficits after traumatic brain injury
In this study, we sought to delineate the dynamics of glycosaminoglycan alterations after TBI and discover the precise biologic processes responsible for observed glycosaminoglycan changes after injury. We performed state-of-the art mass spectrometry on brain tissues isolated from mice after TBI or craniotomy-alone. We observed dynamic changes in glycosaminoglycans at Day 1 and 7 post-TBI, with heparan sulfate, chondroitin sulfate, and hyaluronan remaining significantly increased after a week vis-à-vis craniotomy-alone tissues. We did not observe appreciable changes in circulating glycosaminoglycans in mice after experime...
Source: Matrix Biology - December 22, 2023 Category: Molecular Biology Authors: Kaori Oshima Noah Siddiqui James E Orfila Danelle Carter Justin Laing Xiaorui Han Igor Zakharevich Renato V Iozzo Arsen Ghasabayan Hunter Moore Fuming Zhang Robert J Linhardt Ernest E Moore Nidia Quillinan Eric P Schmidt Paco S Herson Joseph A Hippensteel Source Type: research
