Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility
In this study, we test whether force-induced matrix remodeling can instead be used as a proxy for cellular traction forces. We measure the traction forces of hepatic stellate cells and different glioblastoma cell lines and quantify matrix remodeling by measuring the fiber orientation and fiber density around these cells. In agreement with simulated fiber networks, we demonstrate that changes in local fiber orientation and density are directly related to cell forces. By resolving Rho-kinase (ROCK) Inhibitor-induced changes of traction forces and fiber alignment and density in hepatic stellate cells, we show that the method ...
Source: Matrix Biology - November 15, 2023 Category: Molecular Biology Authors: David B öhringer Andreas Bauer Ivana Moravec Lars Bischof Delf Kah Christoph Mark Thomas J Grundy Ekkehard G örlach Geraldine M O'Neill Silvia Budday Pamela L Strissel Reiner Strick Andrea Malandrino Richard Gerum Michael Mak Martin Rausch Ben Fabry Source Type: research
Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility
In this study, we test whether force-induced matrix remodeling can instead be used as a proxy for cellular traction forces. We measure the traction forces of hepatic stellate cells and different glioblastoma cell lines and quantify matrix remodeling by measuring the fiber orientation and fiber density around these cells. In agreement with simulated fiber networks, we demonstrate that changes in local fiber orientation and density are directly related to cell forces. By resolving Rho-kinase (ROCK) Inhibitor-induced changes of traction forces and fiber alignment and density in hepatic stellate cells, we show that the method ...
Source: Matrix Biology - November 15, 2023 Category: Molecular Biology Authors: David B öhringer Andreas Bauer Ivana Moravec Lars Bischof Delf Kah Christoph Mark Thomas J Grundy Ekkehard G örlach Geraldine M O'Neill Silvia Budday Pamela L Strissel Reiner Strick Andrea Malandrino Richard Gerum Michael Mak Martin Rausch Ben Fabry Source Type: research
Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility
In this study, we test whether force-induced matrix remodeling can instead be used as a proxy for cellular traction forces. We measure the traction forces of hepatic stellate cells and different glioblastoma cell lines and quantify matrix remodeling by measuring the fiber orientation and fiber density around these cells. In agreement with simulated fiber networks, we demonstrate that changes in local fiber orientation and density are directly related to cell forces. By resolving Rho-kinase (ROCK) Inhibitor-induced changes of traction forces and fiber alignment and density in hepatic stellate cells, we show that the method ...
Source: Matrix Biology - November 15, 2023 Category: Molecular Biology Authors: David B öhringer Andreas Bauer Ivana Moravec Lars Bischof Delf Kah Christoph Mark Thomas J Grundy Ekkehard G örlach Geraldine M O'Neill Silvia Budday Pamela L Strissel Reiner Strick Andrea Malandrino Richard Gerum Michael Mak Martin Rausch Ben Fabry Source Type: research
Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility
In this study, we test whether force-induced matrix remodeling can instead be used as a proxy for cellular traction forces. We measure the traction forces of hepatic stellate cells and different glioblastoma cell lines and quantify matrix remodeling by measuring the fiber orientation and fiber density around these cells. In agreement with simulated fiber networks, we demonstrate that changes in local fiber orientation and density are directly related to cell forces. By resolving Rho-kinase (ROCK) Inhibitor-induced changes of traction forces and fiber alignment and density in hepatic stellate cells, we show that the method ...
Source: Matrix Biology - November 15, 2023 Category: Molecular Biology Authors: David B öhringer Andreas Bauer Ivana Moravec Lars Bischof Delf Kah Christoph Mark Thomas J Grundy Ekkehard G örlach Geraldine M O'Neill Silvia Budday Pamela L Strissel Reiner Strick Andrea Malandrino Richard Gerum Michael Mak Martin Rausch Ben Fabry Source Type: research
Fiber alignment in 3D collagen networks as a biophysical marker for cell contractility
In this study, we test whether force-induced matrix remodeling can instead be used as a proxy for cellular traction forces. We measure the traction forces of hepatic stellate cells and different glioblastoma cell lines and quantify matrix remodeling by measuring the fiber orientation and fiber density around these cells. In agreement with simulated fiber networks, we demonstrate that changes in local fiber orientation and density are directly related to cell forces. By resolving Rho-kinase (ROCK) Inhibitor-induced changes of traction forces and fiber alignment and density in hepatic stellate cells, we show that the method ...
Source: Matrix Biology - November 15, 2023 Category: Molecular Biology Authors: David B öhringer Andreas Bauer Ivana Moravec Lars Bischof Delf Kah Christoph Mark Thomas J Grundy Ekkehard G örlach Geraldine M O'Neill Silvia Budday Pamela L Strissel Reiner Strick Andrea Malandrino Richard Gerum Michael Mak Martin Rausch Ben Fabry Source Type: research
TARGETING THE αVβ3/NgR2 PATHWAY IN NEUROENDOCRINE PROSTATE CANCER
Matrix Biol. 2023 Nov 11:S0945-053X(23)00115-4. doi: 10.1016/j.matbio.2023.11.003. Online ahead of print.ABSTRACTHighly aggressive, metastatic, neuroendocrine prostate cancer, which typically develops from prostate cancer cells acquiring resistance to androgen deprivation therapy, is associated with limited treatment options and hence poor prognosis. We have previously demonstrated that the αVβ3 integrin is over-expressed in neuroendocrine prostate cancer. We now show that LM609, a monoclonal antibody that specifically targets the human αVβ3 integrin, hinders the growth of neuroendocrine prostate cancer patient-derived...
Source: Matrix Biology - November 13, 2023 Category: Molecular Biology Authors: Anna Testa Fabio Quaglia Nicole M Naranjo Cecilia E Verrillo Christopher D Shields Stephen Lin Maxwell W Pickles Drini F Hamza Tami Von Schalscha David A Cheresh Benjamin Leiby Qin Liu Jianyi Ding William K Kelly D Craig Hooper Eva Corey Edward F Plow Dar Source Type: research
TARGETING THE αVβ3/NgR2 PATHWAY IN NEUROENDOCRINE PROSTATE CANCER
Matrix Biol. 2023 Nov 11:S0945-053X(23)00115-4. doi: 10.1016/j.matbio.2023.11.003. Online ahead of print.ABSTRACTHighly aggressive, metastatic, neuroendocrine prostate cancer, which typically develops from prostate cancer cells acquiring resistance to androgen deprivation therapy, is associated with limited treatment options and hence poor prognosis. We have previously demonstrated that the αVβ3 integrin is over-expressed in neuroendocrine prostate cancer. We now show that LM609, a monoclonal antibody that specifically targets the human αVβ3 integrin, hinders the growth of neuroendocrine prostate cancer patient-derived...
Source: Matrix Biology - November 13, 2023 Category: Molecular Biology Authors: Anna Testa Fabio Quaglia Nicole M Naranjo Cecilia E Verrillo Christopher D Shields Stephen Lin Maxwell W Pickles Drini F Hamza Tami Von Schalscha David A Cheresh Benjamin Leiby Qin Liu Jianyi Ding William K Kelly D Craig Hooper Eva Corey Edward F Plow Dar Source Type: research
TARGETING THE αVβ3/NgR2 PATHWAY IN NEUROENDOCRINE PROSTATE CANCER
Matrix Biol. 2023 Nov 11:S0945-053X(23)00115-4. doi: 10.1016/j.matbio.2023.11.003. Online ahead of print.ABSTRACTHighly aggressive, metastatic, neuroendocrine prostate cancer, which typically develops from prostate cancer cells acquiring resistance to androgen deprivation therapy, is associated with limited treatment options and hence poor prognosis. We have previously demonstrated that the αVβ3 integrin is over-expressed in neuroendocrine prostate cancer. We now show that LM609, a monoclonal antibody that specifically targets the human αVβ3 integrin, hinders the growth of neuroendocrine prostate cancer patient-derived...
Source: Matrix Biology - November 13, 2023 Category: Molecular Biology Authors: Anna Testa Fabio Quaglia Nicole M Naranjo Cecilia E Verrillo Christopher D Shields Stephen Lin Maxwell W Pickles Drini F Hamza Tami Von Schalscha David A Cheresh Benjamin Leiby Qin Liu Jianyi Ding William K Kelly D Craig Hooper Eva Corey Edward F Plow Dar Source Type: research
Age Related Changes in Hyaluronan Expression leads to Meibomian Gland Dysfunction
Matrix Biol. 2023 Nov 8:S0945-053X(23)00114-2. doi: 10.1016/j.matbio.2023.11.002. Online ahead of print.ABSTRACTThe prevalence of dry eye disease (DED) ranges from ∼5 to 50% and its associated symptoms decrease productivity and reduce the quality of life. Approximately 85% of all DED cases are caused by Meibomian gland dysfunction (MGD). As humans and mice age, their Meibomian glands (MGs) undergo age-related changes resulting in age related-MGD (ARMGD). The precise cause of ARMGD remains elusive, which makes developing therapies extremely challenging. We previously demonstrated that a hyaluronan (HA)-rich matrix exists ...
Source: Matrix Biology - November 10, 2023 Category: Molecular Biology Authors: Sudhir Verma Isabel Y Moreno Mingxia Sun Tarsis Ferreira Gesteira Vivien Coulson- Thomas Source Type: research
Age Related Changes in Hyaluronan Expression leads to Meibomian Gland Dysfunction
Matrix Biol. 2023 Nov 8:S0945-053X(23)00114-2. doi: 10.1016/j.matbio.2023.11.002. Online ahead of print.ABSTRACTThe prevalence of dry eye disease (DED) ranges from ∼5 to 50% and its associated symptoms decrease productivity and reduce the quality of life. Approximately 85% of all DED cases are caused by Meibomian gland dysfunction (MGD). As humans and mice age, their Meibomian glands (MGs) undergo age-related changes resulting in age related-MGD (ARMGD). The precise cause of ARMGD remains elusive, which makes developing therapies extremely challenging. We previously demonstrated that a hyaluronan (HA)-rich matrix exists ...
Source: Matrix Biology - November 10, 2023 Category: Molecular Biology Authors: Sudhir Verma Isabel Y Moreno Mingxia Sun Tarsis Ferreira Gesteira Vivien Coulson- Thomas Source Type: research
TGF- β induces matrisome pathological alterations and EMT in patient-derived prostate cancer tumoroids
Matrix Biol. 2023 Nov 7:S0945-053X(23)00113-0. doi: 10.1016/j.matbio.2023.11.001. Online ahead of print.ABSTRACTExtracellular matrix (ECM) tumorigenic alterations resulting in high matrix deposition and stiffening are hallmarks of adenocarcinomas and are collectively defined as desmoplasia. Here, we thoroughly analysed primary prostate cancer tissues obtained from numerous patients undergoing radical prostatectomy to highlight reproducible structural changes in the ECM leading to the loss of the glandular architecture. Starting from patient cells, we established prostate cancer tumoroids (PCTs) and demonstrated they requir...
Source: Matrix Biology - November 9, 2023 Category: Molecular Biology Authors: Soraia Fernandes Jorge Oliver-De La Cruz Sofia Morazzo Francesco Niro Marco Cassani Helena Ďuríková Alessio Caravella Piergiuseppe Fiore Giulia Azzato Giuseppe De Marco Agostino Lauria Valerio Izzi Veronika Bos áková Jan Fric Petr Filipensky Giancarl Source Type: research
TGF- β induces matrisome pathological alterations and EMT in patient-derived prostate cancer tumoroids
Matrix Biol. 2023 Nov 7:S0945-053X(23)00113-0. doi: 10.1016/j.matbio.2023.11.001. Online ahead of print.ABSTRACTExtracellular matrix (ECM) tumorigenic alterations resulting in high matrix deposition and stiffening are hallmarks of adenocarcinomas and are collectively defined as desmoplasia. Here, we thoroughly analysed primary prostate cancer tissues obtained from numerous patients undergoing radical prostatectomy to highlight reproducible structural changes in the ECM leading to the loss of the glandular architecture. Starting from patient cells, we established prostate cancer tumoroids (PCTs) and demonstrated they requir...
Source: Matrix Biology - November 9, 2023 Category: Molecular Biology Authors: Soraia Fernandes Jorge Oliver-De La Cruz Sofia Morazzo Francesco Niro Marco Cassani Helena Ďuríková Alessio Caravella Piergiuseppe Fiore Giulia Azzato Giuseppe De Marco Agostino Lauria Valerio Izzi Veronika Bos áková Jan Fric Petr Filipensky Giancarl Source Type: research
Pulmonary Fibroelastosis - A Review
Matrix Biol. 2023 Nov 1:S0945-053X(23)00106-3. doi: 10.1016/j.matbio.2023.10.003. Online ahead of print.ABSTRACTElastin is a long-lived fibrous protein that is abundant in the extracellular matrix of the lung. Elastic fibers provide the lung the characteristic elasticity during inhalation with recoil during exhalation thereby ensuring efficient gas exchange. Excessive deposition of elastin and other extracellular matrix proteins reduces lung compliance by impairing ventilation and compromising gas exchange. Notably, the degree of elastosis is associated with the progressive decline in lung function and survival in patients...
Source: Matrix Biology - November 3, 2023 Category: Molecular Biology Authors: Dan J K Yombo Satish Madala Chanukya Pavan Vemulapalli Harshavardhana H Ediga William D Hardie Source Type: research
Pulmonary Fibroelastosis - A Review
Matrix Biol. 2023 Nov 1:S0945-053X(23)00106-3. doi: 10.1016/j.matbio.2023.10.003. Online ahead of print.ABSTRACTElastin is a long-lived fibrous protein that is abundant in the extracellular matrix of the lung. Elastic fibers provide the lung the characteristic elasticity during inhalation with recoil during exhalation thereby ensuring efficient gas exchange. Excessive deposition of elastin and other extracellular matrix proteins reduces lung compliance by impairing ventilation and compromising gas exchange. Notably, the degree of elastosis is associated with the progressive decline in lung function and survival in patients...
Source: Matrix Biology - November 3, 2023 Category: Molecular Biology Authors: Dan J K Yombo Satish Madala Chanukya Pavan Vemulapalli Harshavardhana H Ediga William D Hardie Source Type: research
Pulmonary Fibroelastosis - A Review
Matrix Biol. 2023 Nov 1:S0945-053X(23)00106-3. doi: 10.1016/j.matbio.2023.10.003. Online ahead of print.ABSTRACTElastin is a long-lived fibrous protein that is abundant in the extracellular matrix of the lung. Elastic fibers provide the lung the characteristic elasticity during inhalation with recoil during exhalation thereby ensuring efficient gas exchange. Excessive deposition of elastin and other extracellular matrix proteins reduces lung compliance by impairing ventilation and compromising gas exchange. Notably, the degree of elastosis is associated with the progressive decline in lung function and survival in patients...
Source: Matrix Biology - November 3, 2023 Category: Molecular Biology Authors: Dan J K Yombo Satish Madala Chanukya Pavan Vemulapalli Harshavardhana H Ediga William D Hardie Source Type: research
