A novel long non-coding RNA FOXCUT and mRNA FOXC1 pair promote progression and predict poor prognosis in esophageal squamous cell carcinoma.
In this study, the expression of FOXCUT/FOXC1 was measured in 82 ESCC tissues and adjacent noncancerous tissues by real-time quantitative PCR (qPCR). The prognostic significance of the lncRNA-mRNA gene pair was evaluated using Kaplan-Meier survival analysis and log-rank test. Cell biological experiments were performed in ESCC cell lines to explore their functions in tumor progression. Notably elevated FOXCUT and FOXC1 expression levels were observed in cancerous tissues compared to adjacent noncancerous tissues (86.6% and 84.1%, respectively; P
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Pan F, Yao J, Chen Y, Zhou C, Geng P, Mao H, Fang X Tags: Int J Clin Exp Pathol Source Type: research

A fiber-modified adenovirus co-expressing HSV-TK and Coli.NTR enhances antitumor activities in breast cancer cells.
Abstract Breast cancers especially in late and metastatic stages remain refractory to treatment despite advances in surgical techniques and chemotherapy. Suicide gene therapy based on adenoviral technology will be promising strategies for such advanced diseases. We previously showed that co-expression of herpes simplex virus thymidine kinase (HSV-TK) and Escherichia coli nitroreductase (Coli.NTR) by an hTERT-driven adenovirus vector resulted in additive anti-tumor effects in breast cancer cells in vitro and in vivo. As many tumor tissue and cancer cells express low level of coxsackie-adenovirus receptor (CAR), whi...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zhan Y, Yu B, Wang Z, Zhang Y, Zhang HH, Wu H, Feng X, Geng RS, Kong W, Yu XH Tags: Int J Clin Exp Pathol Source Type: research

PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.
In this study, PDGFR-β (+) PCs was isolated from hemangioma tissue (hemangioma-derived perivascular cells, Hem-PCs) by fluorescence-activated cell sorter. In vitro, Hem-PCs showed fibroblast-like morphology. Immunofluorescence staining and flow cytometry showed Hem-PCs expressed MSCs markers CD105, CD90, CD29 and vimentin, pericyte markers α-SMA and PDGFR-β, stem cell marker CD133, and the adipogenic transcription factor PPAR-γ, but not hematopoietic/endothelial markers CD45, CD34, CD31, and flt-1. In vitro inductions confirmed multi-lineage differentiation of Hem-PCs, especially strong adipogenic pot...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Yuan SM, Guo Y, Zhou XJ, Shen WM, Chen HN Tags: Int J Clin Exp Pathol Source Type: research

Establishment and characterization of a new highly metastatic human osteosarcoma cell line derived from Saos2.
Abstract Osteosarcoma is the most common primary malignancy of bone in adolescents and young adults. There is a shortage of tumorigenic and highly metastatic human osteosarcoma cell lines that can be used for metastasis study. Here we establish and characterize a highly metastatic human osteosarcoma cell line that is derived from Saos2 cell line based on bioluminescence. The occasional pulmonary metastatic cells developed from Saos2 were isolated, harvested, characterized and named Saos2-l. The parental Saos2 and Saos2-l cells were further characterized both in vitro and in vivo. Results showed that Saos2-l cells ...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Du L, Fan Q, Tu B, Yan W, Tang T Tags: Int J Clin Exp Pathol Source Type: research

Androgen deprivation therapy induces androgen receptor-dependent upregulation of Egr1 in prostate cancers.
This study also elucidated the potential mechanism underlying Id1 participation in the progression of prostate cancer. Understanding the key molecular events in the transition from ADPC to AIPC may provide new therapeutic intervention strategies for patients with AIPC. PMID: 25031707 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Xu B, Tang G, Xiao C, Wang L, Yang Q, Sun Y Tags: Int J Clin Exp Pathol Source Type: research

Adiponectin exerts antiproliferative effect on human placenta via modulation of the JNK/c-Jun pathway.
In conclusion, adiponectin inhibits HG-induced proliferation of BeWo cells through down-regulation of JNK/c-jun phosphorylation. PMID: 25031708 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Chen H, Chen H, Wu Y, Liu B, Li Z, Wang Z Tags: Int J Clin Exp Pathol Source Type: research

Sub-chronic lead and cadmium co-induce apoptosis protein expression in liver and kidney of rats.
Abstract The combined subchronic effects of exposure to lead acetate and cadmium chloride on apoptosis protein expression were detected in the liver and kidney of rats to investigate the hazards of environmentally relevant, low-dose exposure to these compounds. The TUNEL assay showed that there were increased numbers of apoptotic cells. Immunohistochemical tests showed increased numbers of positive cells under Bax and caspase-3 protein detection and decreased Bcl-2 protein. Furthermore, mitochondrial injury and increased numbers of apoptotic cells with condensed nuclei were observed by TEM. These results suggested...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Yuan G, Dai S, Yin Z, Lu H, Jia R, Xu J, Song X, Li L, Shu Y, Zhao X, Chen Z, Fan Q, Liang X, He C, Yin L, Lv C, Lei Q, Wang L, Mi Y, Yu X, Zhang M Tags: Int J Clin Exp Pathol Source Type: research

TGF-β1/Smad signaling, MMP-14, and MSC markers in arterial injury: discovery of the molecular basis of restenosis.
In this study, we observed the change in expression and distribution of TGF-β1/Smad signaling pathway proteins, MMP-14, and MSC markers in the process of neointima formation using a rat model for balloon-induced carotid artery injury. We found that the increase in downstream Smad signaling was consistent with the elevation of TGF-β1 levels and MSCs accumulated at the lumen side of neointima. Furthermore, the activation of MMP-14 in the injured artery was preceded by the increase in TGF-β1 levels. Herein, we conclude that MMP-14 induces an elevation in the levels of TGF-β1/Smad signaling proteins in inju...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Pang L, Wei C, Duan J, Zou H, Cao W, Qi Y, Jia W, Hu J, Zhao W, Jiang J, Liang W, Li F Tags: Int J Clin Exp Pathol Source Type: research

High-level expression of HOXB13 is closely associated with tumor angiogenesis and poor prognosis of hepatocellular carcinoma.
Abstract Homeobox B13 (HOXB13) is generally considered as a crucial regulator of terminal cellular differentiation. More recently, the absent or aberrant expression of HOXB13 has been increasingly implicated in cancer development and metastasis. However, the expression of HOXB13 in hepatocellular carcinoma (HCC) and its correlation with tumor angiogenesis and prognosis still remain unclear. The aim of the study was to evaluate the expression of HOXB13 in patients with HCC and explore the relationship of HOXB13 expression with clinicopathologic factors, tumor angiogenesis and prognosis. Immunohistochemistry was per...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zhu JY, Sun QK, Wang W, Jia WD Tags: Int J Clin Exp Pathol Source Type: research

MAGE-A3 is highly expressed in a cancer stem cell-like side population of bladder cancer cells.
In this study, we isolated cancer stem celllike side population (SP) cells from human bladder cancer cell line SW780 by a flow cytometry-based SP technique. SP cells were only about 3.6% of SW780 cells and showed higher expression of ATP-binding cassette sub-family G member 2 (ABCG2) and CD133. In vitro assay of tumor sphere growth as well as in vivo assay of xenograft transplantation confirmed the higher tumorigenicity of isolated SP cells. These data indicated that SP cells were enriched with CSCs of bladder cancer. Furthermore, we determined the expression of melanoma antigen family A, 3 (MAGEA3), one of the most studie...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Yin B, Zeng Y, Liu G, Wang X, Wang P, Song Y Tags: Int J Clin Exp Pathol Source Type: research

Oncogenic roles of carbonic anhydrase IX in human nasopharyngeal carcinoma.
Abstract Carbonic anhydrase IX (CA IX), a hypoxia-inducible protein in tumors, has been shown to be valuable for the prognosis of nasopharyngeal carcinoma (NPC). However, the function and mechanism of CA IX has been not explored in NPC. Here, we found that CA IX was detected at higher levels in NPC cells and tissues than their corresponding partners. Furthermore, the cell growth, migration and invasion in vitro were altered with shRNA or overexpression of CA IX in NPC cells. More importantly, the metastatic ability of NPC cells stably expressing CA IX was significantly enhanced using the hepatic metastasis model o...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Sang Y, Wang L, Tang JJ, Zhang MF, Zhang MX, Liu X, Zhang RH, Kang TB, Chen MY Tags: Int J Clin Exp Pathol Source Type: research

Stability of preclinical models of aggressive renal cell carcinomas.
eau P Abstract Renal-cell carcinomas (RCC) are often resistant to conventional cytotoxic agents. Xenograft models are used for in vivo preclinical studies and drug development. The validity of these studies is highly dependent on the phenotypic and genotypic stability of the models. Here we assessed the stability of six aggressive human RCC xenografted in nude/NMRI mice. We compared the initial samples (P0), first (P1) and fifth (P5) passages for the following criteria: histopathology, immunohistochemistry for CK7, CD10, vimentin and p53, DNA allelic profiles using 10 microsatellites and CGH-array. Next we evaluat...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Varna M, Bousquet G, Ferreira I, Goulard M, El-Bouchtaoui M, Artus PM, Verine J, de Kerviler E, Hernandez L, Leboeuf C, Escudier B, Legrès L, Setterblad N, Soliman H, Feugeas JP, Janin A, Bertheau P Tags: Int J Clin Exp Pathol Source Type: research

Alternative splicing in the variable domain of CaMKIIβ affects the level of F-actin association in developing neurons.
Alternative splicing in the variable domain of CaMKIIβ affects the level of F-actin association in developing neurons. Int J Clin Exp Pathol. 2014;7(6):2963-75 Authors: Zheng J, Redmond L, Xu C, Kuang J, Liao W Abstract The Ca(2+)/calmodulin (CaM)-dependent protein kinase II (CaMKII) β has an essential function in dendritic spines via binding to and reorganization of the actin cytoskeleton during plasticity events not shared by CaMKIIα isoform. CaMKIIβ and CaMKIIα isoforms have remarkable structural differences within the variable region. Three exons (E1, E3, and E4) are pre...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zheng J, Redmond L, Xu C, Kuang J, Liao W Tags: Int J Clin Exp Pathol Source Type: research

Distinct patterns of ALDH1A1 expression predict metastasis and poor outcome of colorectal carcinoma.
In this study, we examined ALDH1A1 expression by immunohistochemistry including 406 cases of primary CRC with corresponding adjacent mucosa, with confirmation of real-time PCR and Western blotting. We found that the expression patterns of ALDH1A1 were heterogeneous in the CRC and corresponding adjacent tissues. We defined the ratio of ALDH1A1 level in adjacent mucosa to that in tumor tissues as RA/C and found that the capabilities of tumor invasion and metastasis in the tumors with RA/C
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Xu SL, Zeng DZ, Dong WG, Ding YQ, Rao J, Duan JJ, Liu Q, Yang J, Zhan N, Liu Y, Hu QP, Zhang X, Cui YH, Kung HF, Yu SC, Bian XW Tags: Int J Clin Exp Pathol Source Type: research

Central circuits regulating the sympathetic outflow to lumbar muscles in spinally transected mice by retrograde transsynaptic transport.
Abstract Despite considerable interest in the mechanisms that control the hyperalgesia associated with muscle inflammation, the CNS descending pathways that coordinate autonomic circuits regulating lumbar muscles are not adequately understood. Here we used both pseudorabies virus (PRV)-614 retrograde transsynaptic tracing and spinally transected method in 33 C57BL/6J mice to map the polysynaptic pathways between lumbar muscle and CNS. Tissues were processed for dual-label immunocytochemical detection between PRV-614 and tryptophan hydroxylase (TPH) or tyrosine hydroxylase (TH)-expressing neurons in CNS. In intact ...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Xiang HB, Liu C, Liu TT, Xiong J Tags: Int J Clin Exp Pathol Source Type: research

HOXA9 regulates angiogenesis in human hypertrophic scars: induction of VEGF secretion by epidermal stem cells.
Abstract Hypertrophic scars are fibroproliferative disorders of excessive wound healing after skin injury. Vascular endothelial growth factor (VEGF)-induced angiogenesis plays a major role in fibrogenesis and hypertrophic scar formation. Over recent years, there has been a major interest in homeobox gene regulation of VEGF-VEGFR mediated angiogenesis in dermal tissue. In the current study, we investigated the role of homeobox genes in the epidermis, for their role in angiogenesis, with a focus on epidermal-mesenchymal interactions. As epidermal stem cells (ESCs) have a central role in epidermal homeostasis, we tes...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Cao PF, Xu YB, Tang JM, Yang RH, Liu XS Tags: Int J Clin Exp Pathol Source Type: research

IMP3 expression is associated with epithelial-mesenchymal transition in breast cancer.
Abstract IMP3 plays an important role in tumor invasion and metastasis, to which epithelial to mesenchymal transition (EMT) also contributes. The purpose of this study was to investigate whether IMP3 can regulate invasion and metastasis through EMT in breast cancers. The protein expression levels of IMP3 and EMT markers were analyzed by immunohistochemistry in 180 paraffin-embedded human breast tissue samples. There was an inverse correlation of IMP3 with E-cadherin protein expression (P = 0.042). IMP3 expression directly correlated with both Slug (P = 0.004) and vimentin (P
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Su P, Hu J, Zhang H, Li W, Jia M, Zhang X, Wu X, Cheng H, Xiang L, Zhou G Tags: Int J Clin Exp Pathol Source Type: research

Overexpression of sineoculis homeobox homolog 1 predicts poor prognosis of hepatocellular carcinoma.
This study explores the role of SIX1 in tumor progression and in the prognostic evaluation of hepatocellular carcinoma (HCC). Real-time PCR, Western blotting analysis, immunofluorescence (IF) staining, and immunohistochemistry (IHC) were performed to examine SIX1 expression in HCC cell line/tissues compared with adjacent non-tumor and normal liver tissues. Statistical analysis was applied to evaluate the correlation between SIX1 overexpression and the clinicopathological features of HCC. Survival rates were calculated using the Kaplan-Meier method, and the relationship between prognostic factors and patient survival was an...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Kong J, Zhou X, Liu S, Jin T, Piao Y, Liu C, Lin Z Tags: Int J Clin Exp Pathol Source Type: research

Effects of vitamin D on renal fibrosis in diabetic nephropathy model rats.
This study is to investigate the effects of vitamin D on renal fibrosis in rat diabetic nephropathy models, as well as the changes and interactions in the expressions of renal fibrogenesis- and inflammation-related genes. Rat diabetic nephropathy models were established by high-fat diets, which were subjected to TGF-β1 manipulation, as well as vitamin D treatment. H&E staining, Masson staining, and TEM detection were performed to assess the effects of vitamin D treatment and/or TGF-β1 manipulation on pathological changes in the renal tissues in these rat diabetic nephropathy models. Immunohistology and real-t...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Tian Y, Lv G, Yang Y, Zhang Y, Yu R, Zhu J, Xiao L, Zhu J Tags: Int J Clin Exp Pathol Source Type: research

ZNF217 is associated with poor prognosis and enhances proliferation and metastasis in ovarian cancer.
Abstract ZNF217 is an alternatively spliced Kruppel-like transcription factor that has recently been implicated to play a role in human carcinogenesis. Here, we used immunohistochemistry (IHC) to show that ZNF217 protein is overexpressed in nearly 60% of ovarian tumor samples. The disease-free survival time was shorter in patients with positive ZNF217 expression than in ZNF217-negative patients (P=0.042). Fluorescence in situ hybridization (FISH) analysis showed ZNF217 genomic amplification in the poorly differentiated tumors, suggesting that ZNF217 is associated with the progression of ovarian cancer. Invasion wa...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Li J, Song L, Qiu Y, Yin A, Zhong M Tags: Int J Clin Exp Pathol Source Type: research

Immunolocalization of MMP-2 and MMP-9 in human rheumatoid synovium.
Abstract Matrix metalloproteinase (MMP)-2 and MMP-9, two important members of the matrix metalloproteinase family, have been shown critical contributions in intra-tumor angiogenesis and invasion of tumor progression, and they might also play important roles in the angiogenesis as well as the pannus formation of rheumatoid arthritis (RA). In the present study, we used the immunohistochemistry, the immunofluorescence staining and the con-focal scanning methods to characterize the immunolocalization of MMP-2 and MMP-9 in RA synovium tissues. Our results showed that both MMP-2 and MMP-9 immunostaining could be found i...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zhou M, Qin S, Chu Y, Wang F, Chen L, Lu Y Tags: Int J Clin Exp Pathol Source Type: research

Bmi-1 regulates epithelial-to-mesenchymal transition to promote migration and invasion of breast cancer cells.
Abstract Breast cancer is a highly invasive and metastatic disease. Recent studies report that breast cancer cells that have undergo epithelial-to-mesenchymal transition (EMT) obtain malignant characteristic, however, the molecular mechanism underlying this transition are poorly understood. Here, we found that over-expression associated with the process of breast cancer and that high B-cell-specific moloney murine leukemia virus insertion site 1 (Bmi-1) levels predict shorter survival of breast cancer patients. We demonstrate that Bmi-1 regulates EMT and the migration of breast cancer cells. RNA interference-media...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Li H, Song F, Chen X, Li Y, Fan J, Wu X Tags: Int J Clin Exp Pathol Source Type: research

LncRNA TSLC1-AS1 is a novel tumor suppressor in glioma.
Abstract Growing evidence demonstrates that long non coding RNAs (lncRNAs) play an important role in cancer origination and progression. A novel lncRNA, TSLC1-AS1, is the antisense transcript of tumor suppressor TSLC1. The expression profile and function of TSLC1-AS1 in glioma were investigated using Real-Time Quantitative PCR and siRNA knockdown. The data showed that TSLC1-AS1 expression was down-regulated in tumor tissues compared with that in adjacent normal tissues, and negatively associated with the WHO criteria of the tumors. Overexpression of TSLC1-AS1 resulted in up-regulation of TSLC1 and significant inhi...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Qin X, Yao J, Geng P, Fu X, Xue J, Zhang Z Tags: Int J Clin Exp Pathol Source Type: research

Lenalidomide after stem-cell transplantation for multiple myeloma: a meta-analysis of randomized controlled trials.
Abstract The efficacy and safety of lenalidomide maintenance therapy after ASCT in patients with MM has been in question. In order to address the issue, we conducted a meta-analysis of two randomized double-blind placebo-controlled studies encompassing 1074 patients treated with lenalidomide or placebo maintenance therapy after ASCT. The predominant clinical outcomes of interest were overall survival (OS), progression-free survival (PFS), and adverse events. There was a marked benefit in PFS with lenalidomide (Odds Ratio [OR] = 2.5, 95% confidence interval [CI] = 1.93 to 3.24). There was statistically non-signific...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Gao M, Gao L, Yang G, Tao Y, Tompkins VS, Wu X, Xu H, Zhan F, Shi J Tags: Int J Clin Exp Pathol Source Type: research

Hypoxia-inducible factor 1 alpha contributes to pulmonary vascular dysfunction in lung ischemia-reperfusion injury.
Abstract The revascularization therapy of pulmonary embolism is associated with ischemia-reperfusion (IR) injury. However, the effect of IR injury on pulmonary arterial endothelial function has not been elucidated. Male Sprague-Dawley rats were divided into a control, an IR and an IR plus hypoxia-inducible factor 1 alpha (HIF-1α) stabilizer DMOG group. We found that the acetylcholine (ACh)-induced relaxation was dramatically reduced in pulmonary arteries from IR-injured rats compared with controls (P
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zhao X, Jin Y, Li H, Wang Z, Zhang W, Feng C Tags: Int J Clin Exp Pathol Source Type: research

The role of pleomorphic adenoma gene-like 2 in gastrointestinal cancer development, progression, and prognosis.
Abstract Numerous previous studies have revealed that pleomorphic adenoma gene-like 2 (PLAGL2) is a transcription factor that is active in cancer progression. The aim of this study was to investigate the role of PLAGL2 in the development, progression and prognosis of gastrointestinal cancer. Immunohistochemical analysis revealed that PLAGL2 was expressed in gastrointestinal tumors and adjacent normal tissues. The expression of PLAGL2 was significantly higher in 225 colorectal cancer tissues than in 66 adjacent non-tumor tissues (P = 0.037). However, expression was not significantly different between 286 gastric tu...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Liu B, Lu C, Song YX, Gao P, Sun JX, Chen XW, Wang MX, Dong YL, Xu HM, Wang ZN Tags: Int J Clin Exp Pathol Source Type: research

Maternal protein restriction alters VEGF signaling and decreases pulmonary alveolar in fetal rats.
In this study, we investigated the effects of protein malnutrition in utero on pulmonary alveolarization and vascular growth of the fetal lung and placentae. Pregnant rats were feed with an isocaloric low-protein diet (8% protein) until delivery. Placenta and fetal lungs were harvested on 20th day of gestation (term 21 days of gestation). Lung index (lung weight as a percentage of body weight), total DNA and protein, radial alveolar count, arteriolar wall thickness, lung maturity and angiogenic factor VEGF were assessed. The lung was hypoplastic in IUGR fetus, evidenced by reduction in lung weight, DNA and protein content....
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Liu X, Lin Y, Tian B, Miao J, Xi C, Liu C Tags: Int J Clin Exp Pathol Source Type: research

Clinicopathological characterization of so-called "cholangiocarcinoma with intraductal papillary growth" with respect to "intraductal papillary neoplasm of bile duct (IPNB)".
In conclusion, a majority of ICC of IG type and BTC of PG type could be regarded as a IPNB lineage, and clinically detectable IPNBs were already a malignant papillary lesion. PMID: 25031730 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Nakanuma Y, Sato Y, Ojima H, Kanai Y, Aishima S, Yamamoto M, Ariizumi S, Furukawa T, Hayashi H, Unno M, Ohta T, Hepatolithiasis Subdivision of Intractable Hepatobiliary Diseases Study Group of Japan (Chairman, Hirohito Tsubouchi) Tags: Int J Clin Exp Pathol Source Type: research

Loss of expression of MHC class I-related chain A (MICA) is a frequent event and predicts poor survival in patients with hepatocellular carcinoma.
Abstract Natural killer (NK) cells are important effector cells for the first line of defense against tumor. Distant MHC class I homolog MICA has been identified as human ligand for NK cell activating receptor NKG2D. Engagement of MICA triggers NK cells and augments antigen-specific CTL anti-tumor immunity. However, the expression level of MICA and its clinical significance in hepatocellular carcinoma remains to be elucidated. In the present study, a hospital-based study cohort of 143 HCC patients was involved. MICA expression levels were determined by immunohistochemical staining. The association of MICA expressi...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zhang J, Xu Z, Zhou X, Zhang H, Yang N, Wu Y, Chen Y, Yang G, Ren T Tags: Int J Clin Exp Pathol Source Type: research

Overexpression of GRB2 is correlated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma.
In conclusion, GRB2 expression status could be as a positive biomarker of ESCC progression and lymph node metastasis. PMID: 25031732 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Li LY, Li EM, Wu ZY, Cao HH, Shen JH, Xu XE, Chen B, Wu JY, Xu LY Tags: Int J Clin Exp Pathol Source Type: research

STAT3 serine 727 phosphorylation influences clinical outcome in glioblastoma.
Abstract Besides STAT3 tyrosine 705 phosphorylation (pTyr705-STAT3), phosphorylation of STAT3 at serine 727 (pSer727-STAT3) is shown to contribute to tumorigenesis and be closely related with resistance to radiotherapy and chemotherapy in glioma, but there is currently no study regarding its relevance to prognosis in glioblastoma (GBM). Here, the expression of phosphorylated STAT3 was detected in tumor specimens from 88 patients with newly diagnosed GBM by immunohistochemistry, the Kaplan-Meier survival curve and COX proportional hazards regression model were applied to estimate its influences on progression-free ...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Lin GS, Chen YP, Lin ZX, Wang XF, Zheng ZQ, Chen L Tags: Int J Clin Exp Pathol Source Type: research

Zinc-finger protein X-linked is a novel predictor of prognosis in patients with colorectal cancer.
In conclusion, our findings for the first time demonstrated that ZFX expression may be associated with the progress of CRC and suggested that ZFX has the potential value to be an effective prognostic predictor for CRC patients. PMID: 25031734 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Yan X, Yan L, Su Z, Zhu Q, Liu S, Jin Z, Wang Y Tags: Int J Clin Exp Pathol Source Type: research

High expression of Twist is associated with tumor aggressiveness and poor prognosis in patients with renal cell carcinoma.
Abstract AIMS: Twist has been reported to play crucial roles for malignant aggressiveness; however, detailed pathological significance of Twist in renal cell carcinoma (RCC) is not fully understood. The present study was to clarify clinical significance and molecular functions of Twist in patients with RCC. METHODS: Twist expression was examined by immunohistochemical techniques in 156 formalin-fixed specimens. Cell proliferation, angiogenesis, and apoptosis were measured as the percentage of Ki-67-positive cells (proliferation index, PI), CD31-stained vessels (microvessel density, MVD), and TUNEL-positive ce...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Ohba K, Miyata Y, Matsuo T, Asai A, Mitsunari K, Shida Y, Kanda S, Sakai H Tags: Int J Clin Exp Pathol Source Type: research

Langerhans cell histiocytosis in Chinese adults: absence of BRAF mutations and increased FOXP3(+) regulatory T cells.
In conclusion, clinical features of LCH in adults are distinct from those in children. Adult LCH has a relatively good prognosis and presents as a benign disease. Immune regulation plays an important role in the pathogenesis of adult LCH. PMID: 25031736 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Tong C, Jia X, Jia Y, He Y Tags: Int J Clin Exp Pathol Source Type: research

High expression of lncRNA MALAT1 suggests a biomarker of poor prognosis in colorectal cancer.
This study sought to investigate the role of the long noncoding RNA MALAT1 in the prognosis of stage II/III colorectal cancer (CRC) patients. METHODS: The expression of MALAT1 was evaluated in cancer tissues from 146 stage II/III CRC patients undergoing radical resection and 23 paired normal colonic mucosa samples using quantitative real-time reverse transcriptase PCR. Differences in the expression of MALAT1 between 23 CRC and paired normal colonic mucosa samples were analysed with the Wilcoxon test. Relationships between the expression level of MALAT1, patient clinicopathological parameters and disease-free survival ...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zheng HT, Shi DB, Wang YW, Li XX, Xu Y, Tripathi P, Gu WL, Cai GX, Cai SJ Tags: Int J Clin Exp Pathol Source Type: research

Maternal serum lead levels and risk of preeclampsia in pregnant women: a cohort study in a maternity hospital, Riyadh, Saudi Arabia.
Abstract Preeclampsia is one of the major cause of maternal morbidity and mortality. Despite numerous studies, the etiology of preeclampsia has not yet been fully elucidated. There has been confliction in results on the role of maternal lead in preeclampsia. Keeping in view with the scarcity of data on role of lead in preeclamptic women of Saudi Arabia and the disparity in earlier findings, the present study was carried out to determine the levels of maternal serum lead in patients with preeclampsia in comparison to normal pregnancy. The study consisted of 120 pregnant women divided into three groups of 40 each, c...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Jameil NA Tags: Int J Clin Exp Pathol Source Type: research

Expression of Wnt-5a and β-catenin in primary hepatocellular carcinoma.
In this study, the roles and clinical significance of Wnt-5a and β-catenin expression were analyzed in primary HCC. Real-time PCR (RT-PCR) analysis of Wnt-5a mRNA expression was performed in 26 fresh HCC samples and the corresponding para-carcinoma tissues. Wnt-5a and β-catenin protein expression was detected by immunohistochemical staining of paraffin-embedded tissues of 85 cases of HCC and corresponding para-carcinoma tissues and 15 cases of hepatic cirrhosis. Results showed that Wnt-5a mRNA levels were significantly higher in HCC tissue than in the para-carcinoma tissue (0.102 ± 0.159 and 0.020 ±...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Li P, Cao Y, Li Y, Zhou L, Liu X, Geng M Tags: Int J Clin Exp Pathol Source Type: research

Homozygous inv(11)(q21q23) and MLL gene rearrangement in two patients with myeloid neoplasms.
We report two very unusual cases of myeloid neoplasms with homozygous inv(11)(q21q23) and biallelic MLL rearrangement. Both patients, a 12-year old boy and a 29-year old woman, presented initially with T lymphoblastic leukemia/lymphoma (T-ALL), achieved complete remission with intensive chemotherapy, then recurred as acute myeloid leukemia in one patient and therapy-related myelodysplastic syndromes in the other patient, 24 and 15 months after initial T-ALL diagnosis, respectively. In both cases, biallelic MLL gene rearrangements were confirmed by fluorescence in situ hybridization. Mastermind like 2 gene was identified as...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Tang G, Lu X, Wang SA, Roney EK, Zhang L, Hu S, Lu G, Medeiros LJ, Patel A Tags: Int J Clin Exp Pathol Source Type: research

Predictors of recurrence in breast cancer subtypes with negative lymph node in a Chinese population.
Abstract To establish a series of objective parameters to predict the risk of relapse from axillary lymph node-negative (ANN) breast cancer, and evaluate the patterns of recurrence according to molecular subtypes, we collected information on 2126 consecutive breast cancer patients operated between 2002 and 2006. In this case-control study, 212 patients experiencing recurrence or breast cancer related death were defined as 'poor group'. Another 212 patients were selected from the remaining cases with stratified sampling method to comprise the 'good group'. Significant differences were found in vascular invasion, gr...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Liu X, Guan Y, Zhang W, Liu S, Liu J, Wang L, Niu Y Tags: Int J Clin Exp Pathol Source Type: research

High expression of GPER1, EGFR and CXCR1 is associated with lymph node metastasis in papillary thyroid carcinoma.
Abstract Clinical and epidemiological studies have shown that estrogen may be involved in the development and progression of papillary thyroid carcinoma (PTC). G protein-coupled estrogen receptor 1 (GPER1) is a novel seven-transmembrane estrogen receptor that functions alongside traditional nuclear estrogen receptors (ERs) to regulate the cellular responses to estrogen. The purpose of this study was to examine GPER1, EGFR and CXCR1 expression in PTC and to assess the association of their expression with clinicopathological indicators. GPER1, EGFR and CXCR1 protein expression in 129 PTCs, 61 nodular hyperplasia and...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Tang C, Yang L, Wang N, Li L, Xu M, Chen GG, Liu ZM Tags: Int J Clin Exp Pathol Source Type: research

Yes-associated protein (YAP) is differentially expressed in tumor and stroma according to the molecular subtype of breast cancer.
In this study, we aimed to clarify the expression profiles of Yes-associated protein (YAP) and phosphorylated YAP (pYAP) protein and to verify the clinical implication of the expression of YAP protein in human breast cancer. We selected 678 cases of formalin-fixed paraffin-embedded (FFPE) breast cancer tissue to construct tissue microarray (TMA) blocks. We performed immunohistochemical staining of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth receptor-2 (HER-2) and Ki-67 and fluorescent in situ hybridization (FISH) assay for HER-2 on the TMA sections and divided breast cancers into molecular su...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Kim SK, Jung WH, Koo JS Tags: Int J Clin Exp Pathol Source Type: research

Concurrent CD44s and STAT3 expression in human clear cell renal cellular carcinoma and its impact on survival.
In this study, 75 pairs of carcinoma and paired adjacent non-tumor renal tissue samples were collected from patients with localized ccRCC who underwent a nephrectomy. The expression levels of CD44s and pSTAT3 were analyzed using immunohistochemistry. Correlations between CD44s/pSTAT3 expression and clinical and pathological characteristics were determined using x(2) test, Kaplan-Meier analysis and Cox's proportional hazards model. We found that CD44s is highly expressed in 46.67% of tumor tissues, and its high expression was significantly associated with high tumor grade (P
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Qin J, Yang B, Xu BQ, Smithc A, Xu L, Yuan JL, Li L Tags: Int J Clin Exp Pathol Source Type: research

Concurrent alterations of RAGE, RECK, and MMP9 protein expression are relevant to Epstein-Barr virus infection, metastasis, and survival in nasopharyngeal carcinoma.
This study aimed to concurrently investigate the expressions of receptor for advanced glycation end products (RAGE), reversion inducing cysteine-rich protein with Kazal motifs (RECK) and matrix metalloproteinase 9 (MMP9) in nasopharyngeal carcinoma (NPC) and their correlations with clinicopathological properties. Using immunohistochemistry, we found that RECK expression was downregulated in NPC tissues compared with chronic nasopharyngitis (CNT) tissues, while RAGE and MMP9 expressions were upregulated. We further found that RECK expression level was inversely correlated with MMP9 expression level in NPC, whereas RAGE expr...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zhou DN, Deng YF, Li RH, Yin P, Ye CS Tags: Int J Clin Exp Pathol Source Type: research

Expression of APC protein during tongue malignant transformation in galectin-3-deficient mice challenged by the carcinogen 4-nitroquniline-n-oxide.
Abstract Galectin-3 (Gal3) has been implicated in the development of different tumors because of its involvement in the Wnt signaling pathway by promoting beta-catenin translocation into the nucleus. The APC protein, a negative regulator of this pathway, has been strongly implicated in the development of colon cancer, but still has an undetermined role in the formation of oral cancer. Therefore, this study aimed to evaluate the relationship between Gal3, the Wnt signaling pathway, and APC expression in dysplasias and carcinomas developed experimentally in mice. Sixty galectin-3-deficient (Gal3(-/-)) and 60 wild-ty...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: de Souza MV, Servato JP, Loyola AM, Cardoso SV, Chammas R, Liu FT, Silva MJ, de Faria PR Tags: Int J Clin Exp Pathol Source Type: research

IgM expression in paraffin sections distinguishes follicular lymphoma from reactive follicular hyperplasia.
Abstract The trapping of IgM-containing immune complexes (ICs) by follicular dendritic cells (FDCs) serves as an important step in promoting germinal center (GC) formation. Thus, the deposition of IgM-containing ICs on FDCs can be detected by antibodies recognizing IgM. The present investigation provides the first comprehensive report on the IgM staining pattern in follicular lymphoma (FL, n = 60), with comparisons to reactive follicular hyperplasias (RFH, n = 25), demonstrating that immunohistochemical staining for IgM in paraffin-embedded sections seems to be an additional tool for differentiating between FL and...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Zheng Y, Zhou X, Xie J, Zhu H, Zhang S, Zhang Y, Wei X, Yue B Tags: Int J Clin Exp Pathol Source Type: research

Clinicopathological significance of aberrant Notch receptors in intrahepatic cholangiocarcinoma.
In this study, clinicopathological significance of aberrant expression of Notch receptors in intrahepatic cholangiocarcinoma (ICC) was investigated. Thus, forty-one ICC specimens were examined by immunohistochemistry using anti-Notch1-4 antibodies, respectively. Expression of Notch receptors was scored by percentage of positive tumor cells and intensity of immunostaining. Clinicopathological parameters and survival data were compared with the expression of Notch receptors, respectively. Expression of Notch receptors was identified in cancer cells, as well as in non-neoplastic cells. Compared with adjacent non-tumor liver t...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Wu WR, Shi XD, Zhang R, Zhu MS, Xu LB, Yu XH, Zeng H, Wang J, Liu C Tags: Int J Clin Exp Pathol Source Type: research

Distribution of lymphomas in Poland according to World Health Organization classification: analysis of 11718 cases from National Histopathological Lymphoma Register project - the Polish Lymphoma Research Group study.
We present the NHLR data and compare lymphoma distribution in Poland, Europe, as well as in North Central and South America. Records of 11718 patients diagnosed in 24 pathology departments from all over the country were retrieved and reclassified into indolent and aggressive lymphomas according to the 2008 revised WHO classification system. DLBCL (32.9%; 2587), CLL/SLL (31.84%; 2504) and MCL (9.04%; 711) were the three most frequent NHL. The ratio of indolent to aggressive NHL was 1.72; 63.25% (4809) to 36.25% (2794) of cases respectively. Multiple myeloma was less frequent as compared to the data from population-based nat...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Szumera-Ciećkiewicz A, Gałązka K, Szpor J, Rymkiewicz G, Jesionek-Kupnicka D, Gruchała A, Ziarkiewicz-Wróblewska B, Poniatowska-Broniek G, Demczuk S, Prochorec-Sobieszek M Tags: Int J Clin Exp Pathol Source Type: research

Decreased expression of miR-204 is associated with poor prognosis in patients with breast cancer.
In conclusion, the miR-204 may be a potential diagnostic and prognostic biomarker of breast cancer. PMID: 25031750 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Li W, Jin X, Zhang Q, Zhang G, Deng X, Ma L Tags: Int J Clin Exp Pathol Source Type: research

S-1 monotherapy as second line chemotherapy in advanced gastric cancer patients previously treated with cisplatin/infusional fluorouracil.
In conclusion, S-1 monotherapy provided a mild response rate and overall survival, and a favorable toxicity profile in the second line setting after the first line failure to cisplatin and fluorouracil combination. PMID: 25031751 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Lv F, Liu X, Wang B, Guo H, Li J, Shen L, Jin M Tags: Int J Clin Exp Pathol Source Type: research

Prognostic value of glomerular C4d staining in patients with IgA nephritis.
Abstract INTRODUCTION: Mesangial IgA deposition is the initiative factor in the pathogenesis of IgA nephropathy (IgAN). Glomerular IgA depositon leads to activation local complement system. C4d positivity shows that complement activation occurs via alternative pathway. C4d positivity at the time of renal biopsy can be associated with poor prognosis in IgA nephropathy. We aimed to evaluate C4d deposition and renal outcome in patients with IgA nephritis. METHODS: Between January 2005 and December 2009, 40 patients with IgA nephritis were enrolled. Renal biopsy specimens of 33 patients have been evaluated. C4d i...
Source: International Journal of Clinical and Experimental Pathology - July 19, 2014 Category: Pathology Authors: Sahin OZ, Yavas H, Taslı F, Gibyeli DG, Ersoy R, Uzum A, Cirit M Tags: Int J Clin Exp Pathol Source Type: research