An unusual case of complicated temporal lobe abscess following tympanomastoidectomy.
We report a unusual case of complicated temporal lobe abscess following tympanomastoidectomy in a 26-year-old Chinese man here. The patient complained of binaural recurrent purulent discharge accompanied by hearing loss more than 10 years, then he received a right tympanomastoidectomy three months ago, but 3 weeks after surgery, he started to experience fierce headache and nausea and so on. The CT and MRI suggested the diagnosis of right temporal lobe abscess and then right temporal lobe abscess was excised. The patient was successfully treated with a right temporal lobe abscess resection and a radical right mastoidectomy....
Source: International Journal of Clinical and Experimental Pathology - July 6, 2013 Category: Pathology Authors: Yin T, Ren J, Lu Y, Chen X, Wang Y, Huang F Tags: Int J Clin Exp Pathol Source Type: research

Diagnosis and treatment of placental site trophoblastic tumor.
Abstract Here we report a case of a placental site trophoblastic tumor in a 36 year old Chinese woman, 31 months following a prior normal pregnancy. Her clinical presentation and ultrasound findings were uncharacteristic; and the final definitive diagnosis was established based on histological examination in conjunction with immunohistochemistry studies and a normal beta human chorionic gonadotropin level. The tumor exhibited high grade histological features with tumor necrosis, nuclear atypia and high mitosis. The patient was successfully treated with hysterectomy with pre- and post-operative chemotherapy. P...
Source: International Journal of Clinical and Experimental Pathology - July 6, 2013 Category: Pathology Authors: Huang F, Zheng W, Liang Q, Yin T Tags: Int J Clin Exp Pathol Source Type: research

Renal cell carcinomas with t(6;11)(p21;q12) presenting with tubulocystic renal cell carcinoma-like features.
In this study, we reported an additional genetically confirmed case of renal cell carcinomas (RCCs) with t(6;11)(p21;q12) showing an unusual histological pattern. Histologically, the tumor was entirely composed of small to intermediate sized tubules and cysts. The tubules and cysts were lined by a single layer of flat, hobnail, cuboidal to columnar epithelial cells. Most cells demonstrated abundant eosinophilic cytoplasm with regular, round or oval nuclei and some inconspicuous nucleoli. All these morphological features are suggestive of tubulocystic carcinoma of the kidney. However, the tumor demonstrated moderately (2+) ...
Source: International Journal of Clinical and Experimental Pathology - July 6, 2013 Category: Pathology Authors: Rao Q, Zhang XM, Tu P, Xia QY, Shen Q, Zhou XJ, Shi QL Tags: Int J Clin Exp Pathol Source Type: research

Invasion of primary glioma- and cell line-derived spheroids implanted into corticostriatal slice cultures.
In conclusion, the invasion of gliomas is preserved using primary glioma spheroids. Some stem cell features are preserved as well, making this model useful in drug development elucidating both invasion and cancer stemness at the early in vitro level. PMID: 23573302 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Aaberg-Jessen C, Nørregaard A, Christensen K, Pedersen CB, Andersen C, Kristensen BW Tags: Int J Clin Exp Pathol Source Type: research

Identification of CD68(+) neutrophil granulocytes in in vitro model of acute inflammation and inflammatory bowel disease.
Abstract CD-68 is widely regarded as a selective marker for human monocytes and macrophages and is commonly used in human pathology studies. The purpose of this study was to investigate the expression of CD-68 in human peripheral blood mononuclear cells (PBMCs), neutrophil granulocytes (NGs) and in inflamed intestinal tissue samples for comparison. PBMCs and NGs were isolated from heparinized human blood samples. Intestinal biopsies were obtained during routine endoscopic procedures from patients with inflammatory bowel disease (IBD), e.g. ulcerative colitis and Crohn's disease. Gene and protein expression was ana...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Amanzada A, Malik IA, Blaschke M, Khan S, Rahman H, Ramadori G, Moriconi F Tags: Int J Clin Exp Pathol Source Type: research

Human fetal ductal plate revisited: II. MUC1, MUC5AC, and MUC6 are expressed in human fetal ductal plate and MUC1 is expressed also in remodeling ductal plate, remodeled ductal plate and mature bile ducts of human fetal livers.
Abstract Mucins are high-molecular-weight glycoproteins, which are heavily decorated with a large number of O-linked oligosaccharides and a few N-glycan chains, linked to a protein backbone. The protein backbone is called mucin core protein or MUC apomucins. MUC expression is down-regulated or up-regulated in malignant neoplasms. These alterations of MUC apomucins, which are regulated by MUC genes, are associated with carcinogenesis and malignant potentials of cancers. MUC expression during human fetal intrahepatic bile duct (IBD) development has been studied only once, and there has been only one histochemical st...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Overexpression of both VEGF-A and VEGF-C in gastric cancer correlates with prognosis, and silencing of both is effective to inhibit cancer growth.
Abstract BACKGROUND: Vascular endothelial growth factor (VEGF)-A and VEGF-C are two important molecules involving in tumor development and metastasis via angiogenesis and lymphangiogenesis. However, the combined effect of VEGF-A and VEGF-C on the growth of gastric cancer (GC) is not clear. METHODS: The correlations of VEGF-A and VEGF-C expressions with clinicopathologic parameters and prognosis were evaluated in patients with GC. Furthermore, lentivirus-mediated RNA interfering (RNAi) targeting VEGF-A and/or VEGF-C was employed to silence their expressions in SGC7901 GC cell line. Cell proliferation and apopt...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Wang X, Chen X, Fang J, Yang C Tags: Int J Clin Exp Pathol Source Type: research

FER overexpression is associated with poor postoperative prognosis and cancer-cell survival in non-small cell lung cancer.
Abstract Here, we show that overexpression of fer tyrosine kinase (FER), a non-receptor tyrosine kinase, predicts poor postoperative outcome and might be involved in cancer-cell survival in non-small cell lung cancer (NSCLC). Systematic screening using in silico analyses and quantitative RT-PCR revealed that FER was overexpressed in about 10% of NSCLC patients. Evaluation of FER expression using immunohistochemistry (IHC) on tissue microarrays was consistent with the mRNA level detected using quantitative RT-PCR. In analyses of 135 NSCLC patients who had undergone potential curative resection, we found that FER ov...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Kawakami M, Morita S, Sunohara M, Amano Y, Ishikawa R, Watanabe K, Hamano E, Ohishi N, Nakajima J, Yatomi Y, Nagase T, Fukayama M, Takai D Tags: Int J Clin Exp Pathol Source Type: research

An immunohistochemical study of primary signet-ring cell carcinoma of the stomach and colorectum: II. Expression of MUC1, MUC2, MUC5AC, and MUC6 in normal mucosa and in 42 cases.
Abstract Expression of MUC apomucins has rarely been investigated in the signet-ring cell carcinoma (SRCC) of the stomach and colorectum. The author examined immunohistochemically the expression status of MUC1, MUC2, MUC5AC, and MUC6 in 30 cases of gastric SRCC and 12 cases of colorectal SRCC. The normal distribution of these MUC apomucins was also examined in the non-tumorous parts of the stomach and colorectum. In normal tissues, the stomach epithelial cells consistently expressed MUC2, MUC5AC, MUC6, but consistently not MUC1. In colorectum, cryptal epithelial cells consistently expressed MUC2, but consistently ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Ferritin L and Ferritin H are differentially located within hepatic and extra hepatic organs under physiological and acute phase conditions.
Abstract Ferritin L (FTL) and Ferritin H (FTH) subunits are responsible for intercellular iron storage. We previously reported increasing amounts of liver cytoplasmic and nuclear iron content during acute phase response (APR). Aim of the present study is to demonstrate intracellular localization of ferritin subunits in liver compared with extra hepatic organs of rat under physiological and acute phase conditions. Rats were administered turpentine-oil (TO) intramuscularly to induce a sterile abscess (acute-phase-model) and sacrificed at different time points. Immunohistochemistry was performed utilizing horse-reddi...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Ahmad S, Moriconi F, Naz N, Sultan S, Sheikh N, Ramadori G, Malik IA Tags: Int J Clin Exp Pathol Source Type: research

An immunohistochemical study of primary signet-ring cell carcinoma of the stomach and colorectum: III. Expressions of EMA, CEA, CA19-9, CDX-2, p53, Ki-67 antigen, TTF-1, vimentin, and p63 in normal mucosa and in 42 cases.
Abstract There have no comprehensive immunohistochemical studies of primary signet ring cell carcinoma (SRCC) in the stomach and colorectum. The author examined the expression of nine common antigens (EMA, CEA, CA19-9, CDX-2, p53, Ki-67 antigen, TTF-1, vimentin, and p63) in the non-tumorous normal epithelium of the stomach and colorectum and in 42 cases of primary SRCC of the stomach (30 cases) and colorectum (12 cases). The normal epithelium of the stomach and colon consistently (100%) expressed EMA, CEA, CA19-9, CDX-2, and Ki-67 (labeling
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Effects on high cholesterol-fed to liver, retina, hippocampus, and Harderian gland in Goto-Kakizaki rat.
Abstract To understand the relationship among cholesterolemia, hyperglycemic stage in non obese type 2 diabetes mellitus, and histological perturbations on liver, retina, hippocampus, and Harderian gland, we maintained rat on a diet high in cholesterol for fourteen weeks, then analyzed blood lipid profiles, blood glucose, hepatic enzymes, and microscopic lesion of those tissues. We observed that high cholesterol-treated rat elevated in cholesterol and low density lipoprotein with not correlated to hyperglycemia. Histopathological changing in Goto-Kakizaki rat on liver (microvesicular steatosis) and Harderain gland...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Kengkoom K, Klinkhamhom A, Sirimontaporn A, Singha O, Ketjareon T, Panavechkijkul Y, Seriwatanachai D, Ukong S, Ampawong S Tags: Int J Clin Exp Pathol Source Type: research

Gestational diabetes induces chronic hypoxia stress and excessive inflammatory response in murine placenta.
Abstract Metabolic impairments in maternal obesity and gestational diabetes mellitus (GDM) induce an abnormal environment in peripheral blood and cause vascular structure alterations which affect the placental development and function. A GDM model was developed using C57BL/6J female mice fed with high fat food (HF) (40% energy from fat) and a control group with control food (CF) (14% energy from fat) for 14 weeks before mating and throughout the gestation period. A subset of dams was sacrificed at gestational day (GD) 18.5 to evaluate the fetal and placental development. HF-fed dams exhibited significant increase ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Li HP, Chen X, Li MQ Tags: Int J Clin Exp Pathol Source Type: research

A RTK-based functional RNAi screen reveals determinants of PTX-3 expression.
CONCLUSION: Altogether, our study demonstrates the role of RTKs in the regulation of PTX-3 expression and uncovers a potential cardiotoxicity effect of RTK inhibitor treatments in cancer patients who have symptoms of atherosclerosis or are at the risk of atherosclerosis. PMID: 23573312 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Liu H, Qu XK, Yuan F, Zhang M, Fang WY Tags: Int J Clin Exp Pathol Source Type: research

Upregulation of URI/RMP gene expression in cervical cancer by high-throughput tissue microarray analysis.
Abstract URI, or RMP, is a RNA polymerase II subunit RPB5-associated protein known to play essential roles in ubiquitination and transcription. Recently, we and others have shown that URI/RMP is also important for progression of hepatocellular carcinoma, ovarian, and prostate cancers. To identify the mechanistic basis of URI/RMP during multiple cellular processes, we investigated URI/RMP expression in a tissue microarray (TMA) containing multiple normal human tissues. The results showed that URI/RMP is ubiquitously but differentially expressed in these human tissues which partially explains its multiple cellular f...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Gu J, Li X, Liang Y, Qiao L, Ran D, Lu Y, Li X, Wei W, Zheng Q Tags: Int J Clin Exp Pathol Source Type: research

Met-CCL5 modifies monocyte subpopulations during liver fibrosis regression.
Abstract Fibrosis or scarring of the liver parenchyma is a mainstay of chronic liver diseases and is associated with increased morbidity and mortality. Since complete scarring of the liver develops over several decades, therapeutic intervention with the aim of ameliorating fibrosis is of great clinical interest. In a recent study, we could identify the chemokine receptor antagonist Met-CCL5 as a potential compound to inhibit fibrosis progression and accelerate its regression. In the current study we characterized immune changes during fibrosis regression associated with the treatment with the CCL5 (RANTES) chemoki...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Stock MK, Hammerich L, do O NT, Berres ML, Alsamman M, Heinrichs D, Nellen A, Trautwein C, Tacke F, Wasmuth HE, Sahin H Tags: Int J Clin Exp Pathol Source Type: research

Androgen-STAT3 activation may contribute to gender disparity in human simply renal cysts.
CONCLUSIONS: Our data raised the possible novel evidence that androgen-STAT3 activation might contribute to gender disparity in human SRC disease and clarification the esoteric mechanisms will provide us attractive therapy target for cystic kidney disease. PMID: 23573315 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Liu M, Xu YF, Feng Y, Zhai W, Che JP, Xia SQ, Wang GC, Zheng JH Tags: Int J Clin Exp Pathol Source Type: research

Clinical characteristics and treatment of angiomatous meningiomas: a report of 27 cases.
In conclusion, angiomatous meningiomas have relative high male to female ratio, more frequent peritumoral edema, and rich blood vessels. Gross total resection is still the treatment of choice. These patients with residual tumor after surgery can benefit from radiation therapy. Overall, the prognosis of AMs are as good as other benign meningiomas. PMID: 23573316 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Liu Z, Wang C, Wang H, Wang Y, Li JY, Liu Y Tags: Int J Clin Exp Pathol Source Type: research

An immunohistochemical study of primary signet-ring cell carcinoma of the stomach and colorectum: I. Cytokeratin profile in 42 cases.
Abstract Expression of cytokeratin (CK) profiles in primary signet-ring cell carcinoma (SRCC) of the stomach and colorectum have rarely reported; only two such studies are present in the world literature. Herein, an immunohistochemical study on cytokeratin (CK) expression was performed in 42 cases of primary SRCC of the stomach (30 cases) and colorectum (12 cases). SRCC was defined as an adenocarcinoma in which more than 50% adenocarcinoma cells showed SRCC phenotype with prominent intracytoplasmic mucins. In the gastric SRCC, the expression of CK was as follows; CK AE1/3 (30/30, 100%) CK CAM5.2 (30/30, 100%), CK ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

UNC51-like kinase 1 as a potential prognostic biomarker for hepatocellular carcinoma.
Abstract Autophagy is a fundamental cell biological process that confers stress tolerance, limits damage, and sustains viability under adverse conditions. Defective autophagy is associated with diverse diseases. The study aimed to investigate the relationship between UNC51-like kinase 1 (ULK1) expression and clinicopathological characteristics as well as survival in patients with hepatocellular carcinoma (HCC). Expression levels of ULK1 in 55 paired HCC and paracancerous tissues were examined using immunohistochemistry. Although not statistically significant, the expression of ULK1 in adjacent peritumoural tissue ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Xu H, Yu H, Zhang X, Shen X, Zhang K, Sheng H, Dai S, Gao H Tags: Int J Clin Exp Pathol Source Type: research

The effect of accompanying dominant follicle development/ovulation on the outcomes of frozen-thawed blastocyst transfer in HRT cycle.
In this study, we compared the difference between the outcome of frozen-thawed blastula transfer in hormone replacement treatment cycle (HRT) with or without dominant follicle development/ovulation. A total of 171 cases of frozen-thawed blastula transferred successfully in HRT cycle were retrospectively analyzed. Patients were divided into three groups according to dominant follicle development, ovulation or not: Group A, cycles without dominant follicle developing. Group B, cycles with dominant follicle developing but without ovulation. Group C, ovulated cycles. The results showed that there was no significant difference ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Yang X, Dong X, Huang K, Wang L, Xiong T, Ji L, Zhang H Tags: Int J Clin Exp Pathol Source Type: research

Multiple cytokeratin-negative malignant tumors composed only of rhabdoid cells in the renal pelvis: a sarcomatoid urothelial carcinoma?
Abstract The author presents a unique case of multiple cytokeratin-negative malignant tumors consisting only of rhabdoid cells in the renal pelvis. A 54-year-old man complained of hematuria. A transurethral endoscopic examination revealed multiple papillary tumors, and transurethral resection of the bladder tumors was performed. Pathologically, they were ordinary papillary urothelial transitional cell carcinomas. Imaging modalities revealed multiple tumors of the right renal pelvis, and nephrectomy was performed. Grossly, three polypoid tumors measuring 2-4 cm were present in the pelvis. Histologically, they were ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Primary large cell neuroendocrine carcinoma of the ureter.
Abstract Large cell neuroendocrine carcinoma (LCNEC) is the rarest type of urinary tract malignancy. Herein, we report a case of LCNEC that arose in the ureter of a 78-year-old Japanese man with a history of ascending colon cancer that had been excised by a right hemicolectomy. Left-sided hydronephrosis associated with the ureteral tumor was discovered during follow-up. A left nephroureterectomy combined with a partial resection of the urinary bladder was performed because atypical cells were detected using voided urine cytology. A histopathological examination revealed that the ureteral tumor contained large atyp...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Oshiro H, Odagaki Y, Iobe H, Ozu C, Takizawa I, Nagai T, Matsubayashi J, Inagaki A, Miyake S, Nagao T Tags: Int J Clin Exp Pathol Source Type: research

Combined hepatocellular-cholangiocarcinoma with stem cell features, ductal plate malformation subtype: a case report and proposal of a new subtype.
Abstract In the current WHO blue book, combined hepatocellular-cholangiocarcinoma (C-HCC-CC) was classified into two types; classical type and type with stem cell features. The latter is extremely rare, and is subcategorized into the following three subtypes; typical subtype, intermediate cell subtype, and cholangiocellular subtype. Recently, intrahepatic cholangiocarcinoma (ICC) with features of ductal plate malformations (DPM) have been reported, and the ICC with DPM was proposed as a subtype of ICC. The author herein reports a case of C-HCC-CC with stem cell features. Characteristically, the CC element showed f...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Mycosis fungoides in plaque stage with pronounced eosinophilic infiltration, folliculotropism, and concomitant invasive squamous cell carcinoma.
Abstract Mycosis fungoides (MF) is a relatively rare cutaneous T-cell malignancy. Only two cases of MF with marked eosinophilia have been reported. In addition, MF with concomitant squamous cell carcinoma (SCC) occurring in the site of MF has not been reported. The author reports herein a very rare case of MF in the plaque stage showing pronounced eosinophilic infiltration, folliculotropic pattern, and in situ development of poorly differentiated squamous cell carcinoma (SCC). A 75-year-old man was found to show high prostate specific antigen (PSA, 13 hg/ml) and prostatic biopsy showed well differentiated prostati...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Anastomosing hemangioma of the kidney: a case report of a rare subtype of hemangioma mimicking angiosarcoma and review of the literature.
Abstract Anastomosing hemangioma is a recently described, unusual variant of capillary hemangioma which seems to be unique for the genitourinary system, with a particular proclivity for the kidney. Histologically, it is characterized by a unique sinusoidal architecture reminiscent of splenic parenchyma that can lead to concern for angiosarcoma. We herein report a further case of anastomosing hemangioma originating in the right kidney of a 48-year-old Chinese man. The patient had a past medical history significant for hepatocellular carcinoma; this tumor was incidentally identified as an asymptomatic right renal ma...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Zhao M, Li C, Zheng J, Sun K Tags: Int J Clin Exp Pathol Source Type: research

Inverted variant of urothelial carcinoma of the urinary bladder: a report of three cases and a proposal for a new clinicopathologic entity.
In conclusion, the IV and UC were structurally and cytologically very different from the NV of UC. The IV of UC was structurally similar to IP, but cellular atypia and thickened trabeculae were seen in IV and UC. p53 expression and Ki-67 labeling status were entirely different between in IV of UC and IP. The author proposes the term of IV of UC as a new clinicopathological entity. PMID: 23573325 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Inflammatory angiomyolipoma of the liver: an unusual case suggesting relationship to IgG4-related pseudotumor.
l B Abstract Hepatic angiomyolipoma (AML) is rare. Based on its wide histomorphological range, several distinctive histological variants have been delineated. However, hepatic AML displaying predominantly or exclusively inflammatory pattern closely mimicking inflammatory pseudotumor (IPT) is exceptionally rare with only 7 cases reported so far. We herein describe a new case of hepatic inflammatory AML in a 51-year-old woman who presented with unexplained constitutional symptoms suggesting an infectious disease. A liver mass was detected during imaging examination and resected (4.3 cm in maximum diameter). The pati...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Agaimy A, Märkl B Tags: Int J Clin Exp Pathol Source Type: research

Small cell carcinoma of the oral cavity (cheek mucosa): a case report with an immunohistochemical and molecular genetic analysis.
Abstract Small cell carcinoma (SCC) of the oral cavity is extremely rare; only one case has been reported in the English Literature. The author herein reports the second case of SCC of the oral cavity. A 59-year-old man presented with oral tumor (5 cm) in the right cheek mucosa. A biopsy was taken. The HE histology was typical SCC consisting of small epithelial cells with hyperchromatic nuclei, molded nuclei, scant nucleocytoplasmic ratio, and negative nucleoli. Immunohistochemically, the tumor cells are positive for pancytokeratin (PCK) WSS, PCK MNF-116, cytokeratin (CK) 34BE12, CK5/6, CK14, vimentin, KIT (CD117)...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Double-hit and triple-hit lymphomas arising from follicular lymphoma following acquisition of MYC: report of two cases and literature review.
Abstract Double-hit or triple-hit B-cell lymphomas (DHL and THL) are rare subtype lymphomas usually associated with poor prognosis. It is defined by two or three recurrent chromosome translocations; MYC/8q24 loci, usually in combination with the t (14; 18) (q32; q21) bcl-2 gene or/and BCL6/3q27 chromosomal translocation. DHL was often observed both in de-novo diffuse large B cell lymphomas (DLBCL). It is otherwise unclassifiable, showing features intermediate that of large B-cell lymphoma and Burkitt lymphoma. Here, we present two follicular lymphoma patients; one transformed to THL, another transformed to DHL. Bo...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Xu X, Zhang L, Wang Y, Zhang Q, Zhang L, Sun B, Zhang Y Tags: Int J Clin Exp Pathol Source Type: research

Sarcomatoid carcinoma in the pelvic cavity.
Abstract Sarcomatoid carcinoma in the pelvic cavity is very rare. A 58-year-old Japanese man was admitted to our hospital because of lower abdominal fullness. CT and MRI revealed a large mass in the left pelvic cavity. Transurethral bladder endoscopy showed tumor invasion, and large biopsies were obtained from the bladder lesion. Histologically, the tumor was composed of malignant round cells with hyperchromatic nuclei. Many intracytoplasmic vacuoles were present. No carcinomatous areas were seen. Immunohistochemically, the tumor cells were positive for cytokeratin (CK) 18, vimentin, p53 and Ki-67 (labeling 80%). ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Cytokeratin positivity in anaplastic large cell lymphoma: a potential diagnostic pitfall in misdiagnosis of metastatic carcinoma.
Abstract Cytokeratin (pan) and epithelial membrane antigen (EMA) were considered as commonly useful epithelial markers to distinguish cancer from lymphoma, but the expression of cytokeratin (pan) and EMA had also been seen in some lymphomas. Here, we presented an unusual case of anaplastic large cell lymphoma (ALCL) absence of some lymphoid markers (Including CD3, CD20, Pax-5, CD45Ro) and positive for cytokeratin (pan) and EMA, which was misdiagnosed as metastatic carcinoma. Our case suggested that the epithelial markers (including cytokeratin and EMA) show an overlap with that of ALCL, which represented a diagnos...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Zhang Q, Ming J, Zhang S, Li B, Han X, Qiu X Tags: Int J Clin Exp Pathol Source Type: research

Primary cutaneous neuroendocrine tumor (atypical carcinoid) expressing KIT and PDGFRA with myoepithelial differentiation: a case report with immunohistochemical and molecular genetic studies.
Abstract Primary cutaneous neuroendocrine tumors (NET) except for Merkel cell carcinoma have rarely been reported. Herein reported is a very unique case of primary cutaneous NET with immunohistochemical markers of myoepitheliomas. A 47-year-old woman presented a tumor measuring 0.8x0.9x0.6 cm of the face. The tumor was excised completely with wide margins. Morphologically, the tumor was located in the dermis, and the tumor was composed of epithelioid cells arranged in trabecular, sinusoidal, rosette, ribbon-like, and cord-like patterns. Focal areas show tubular formations. The tumor cells were homogenous, and thei...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Aberrant alteration of vascular endothelial growth factor-family signaling in human tubal ectopic pregnancy: what is known and unknown?
Abstract More than 98% of ectopic pregnancies occur in the Fallopian tube. Because many facets of tubal ectopic pregnancy remain unclear, prediction, prevention and treatment of tubal ectopic pregnancy are still a major clinical challenge. Compelling evidence suggests that angiogenic growth factors are involved in normal and abnormal implantation. While acknowledging the importance of an intrauterine pregnancy requires the development of a local blood supply and angiogenesis, we hypothesize that the hypoxic- and estrogen-dependent regulation of vascular endothelial growth factor/placental growth factor expression,...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Shao R, Hu J, Feng Y, Billig H Tags: Int J Clin Exp Pathol Source Type: research

Unusual extrahepatic metastatic sites from hepatocellular carcinoma.
Abstract The author examined unusual extrahepatic metastatic sites from liver hepatocellular carcinomas (HCCs) in autopsy and surgical files at our laboratory in the last 10 years. In autopsy cases (n=31), extrahepatic metastases were present in 21 cases (68%). The most common metastatic sites were lung (n=18). Unusual extrahepatic metastatic sites in the autopsy series were abdominal regional lymph nodes (n=6), bones (n=5), diaphragma (n=2), pancreas (n=2), gall bladder (n=1), stomach (n=1), colon (n=1), adrenal gland (n=1), pleura (n=1), peritoneum (n=1), cervical lymph nodes (n=1), and shoulder soft tissue (n=1...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T, Maruo H Tags: Int J Clin Exp Pathol Source Type: research

Effects of glycyl-glutamine dipeptide supplementation on myocardial damage and cardiac function in rats after severe burn injury.
Abstract Glutamine decreases myocardial damage in ischemia/reperfusion injury. However, the cardioprotective effect of glutamine after burn injury remains unclear. Present study was to explore the protective effect of glycyl-glutamine dipeptide on myocardial damage in severe burn rats. Seventy-two Wistar rats were randomly divided into three groups: normal control (C), burned control (B) and glycyl-glutamine dipeptide-treated (GG) groups. B and GG groups were inflicted with 30% total body surface area of full thickness burn. The GG group was given 1.5 g/kg glycyl-glutamine dipeptide per day and the B group was giv...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Zhang Y, Yan H, Lv SG, Wang L, Liang GP, Wan QX, Peng X Tags: Int J Clin Exp Pathol Source Type: research

Protective effect of mesenchymal stem cell-conditioned medium on hepatic cell apoptosis after acute liver injury.
Abstract The aim of this study was to investigate the role of Mesenchymal Stem Cell (MSC) conditioned medium (CMMSC) on apoptosis of cultured mouse primary hepatocytes after in vivo carbon tetrachloride (CCl4)-induced acute liver injury. The acute liver injury was induced by injecting CCl4 intraperitoneally in C57/BL6 mice. Hepatocytes were isolated by liver perfusion, cultured in a defined medium to maintain their differentiation and characterized by reverse transcriptase polymerase chain reaction (RT-PCR) using the hepatic cell specific genes albumin, hepatocyte nuclear factor 4 (HNF4) and cytokeratin 18 (CK18)....
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Xagorari A, Siotou E, Yiangou M, Tsolaki E, Bougiouklis D, Sakkas L, Fassas A, Anagnostopoulos A Tags: Int J Clin Exp Pathol Source Type: research

Overexpression of integrin a2 promotes osteogenic differentiation of hBMSCs from senile osteoporosis through the ERK pathway.
CONCLUSION: Taken together, our results show that hBMSCs obtained from senile osteoporotic patients gradually lose their capability to differentiate along the osteogenic lineage and proliferate, which might be associated with the abnormal regulation of the integrin α2/ERK/Runx2 signaling pathway undergoing senile osteoporosis. PMID: 23638215 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Hu HM, Yang L, Wang Z, Liu YW, Fan JZ, Fan J, Liu J, Luo ZJ Tags: Int J Clin Exp Pathol Source Type: research

Galectin-3: a novel protein in cerebellar hemangioblastoma.
In conclusion, the development of HB is multi-factorial and the expression of galectin-3 correlates with the expression of HIF-1α and VEGF. Galectin-3 can be used as a marker for the diagnosis of HB as well as it can be a valuable candidate for future targeting immunotherapy. PMID: 23638216 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Al-Salam S, Al-Salam M, Ashari MA Tags: Int J Clin Exp Pathol Source Type: research

Knocking down SMC1A inhibits growth and leads to G2/M arrest in human glioma cells.
In this study, we investigated the role of SMC1A in human glioma. We found that SMC1A was expressed at abnormally high levels in human glioma tissue and in cultured U251 glioma cells. Knocking down SMC1A expression in U251 cells with SMC1A-targeted interfering RNAs inhibited cell growth and induced G2/M cell cycle arrest. Furthermore, expression of the cell cycle associated gene CCNB1IP1 was dramatically increased, whereas expression of Cyclin B1 was decreased in SMC1A-deficienct U251 cells. These results suggest that SMC1A upregulation is involved in the pathogenesis of glioma. PMID: 23638217 [PubMed - in process] (S...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Ma Z, Lin M, Li K, Fu Y, Liu X, Yang D, Zhao Y, Zheng J, Sun B Tags: Int J Clin Exp Pathol Source Type: research

RNAi screening identifies KAT8 as a key molecule important for cancer cell survival.
Abstract Histone acetyltransferases (HATs) regulate many critical cancer events, including transcriptional regulation of oncogene and tumor suppressors, chromatin structure and DNA damage response. Abnormal expression of HATs has been reported in a number of cancers. However, cellular functions of HATs in cancer and molecular mechanisms remain largely unclear. Here, we performed a lentiviral vector-mediated RNAi screen to systematically address the function of HATs in lung cancer cell growth and viability. We identified 8 HATs genes involved in A549 cell viability. Further experiments showed that KAT8 regulates G2...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Zhang S, Liu X, Zhang Y, Cheng Y, Li Y Tags: Int J Clin Exp Pathol Source Type: research

Papillary meningioma: clinical and histopathological observations.
This study investigated by retrospective analysis the clinical, radiological and histopathological findings of 17 papillary meningioma patients who underwent surgical resection or biopsy, to assess the characteristics of papillary meningioma. Eight female and nine male patients were included, with a mean age of 40 (range: 6 to 55) years. Tumors were mostly located in the cerebral convexity and showed irregular margins, absence of a peritumoral rim, heterogeneous enhancement and severe peritumoral brain edema on preoperative images. Brain invasion was often confirmed during the operations, with abundant to exceedingly abund...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Wang DJ, Zheng MZ, Gong Y, Xie Q, Wang Y, Cheng HX, Mao Y, Zhong P, Che XM, Jiang CC, Huang FP, Zheng K, Li SQ, Gu YX, Bao WM, Yang BJ, Wu JS, Xie LQ, Tang HL, Zhu HD, Chen XC, Zhou LF Tags: Int J Clin Exp Pathol Source Type: research

Ductal plate in hepatoblasts in human fetal livers: I. ductal plate-like structures with cytokeratins 7 and 19 are occasionally seen within human fetal hepatoblasts.
Abstract The author has studied the organogenesis of human intrahepatic bile duct in fetal livers. The developmental studies of the liver have focused mainly on the development of intrahepatic bile ducts including the ductal plate (DP). The DP is a single or double layered structures composed of small cuboidal cells and located in the interface between the hepatoblasts and portal mesenchyme. Herein, the author discovered the DP within the parenchymal hepatocytes. The DP-like structures within the hepatoblasts were found in 17 human fetal livers out of the 32 human fetal livers. The gestational ages of the 17 fetal...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Inhibitory effect of bone morphogenetic protein-7 on hepatic fibrosis in rats.
In this study, we aim to investigate the potential effect of BMP-7 on hepatic fibrosis in rats. METHODS: Sprague-Dawley rats were randomly divided into five groups. In the hepatic fibrosis model group (n=8), rats was treated with porcine serum at 0.5ml each time, twice a week. In the negative control group (n=10), rats were intraperitoneally injected with equal amount and frequency saline. Rats were injected with BMP-7 (100 μg/kg weight) before porcine serum intraperitoneal injection in the preventive group (n=9). For the early (n=10) and late (n=8) treatment group, rats were received with BMP-7 (100 μg/kg weigh...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Wang SL, Yang CQ, Qi XL, Yuan M, Chang YZ, Yang L, Gao HJ Tags: Int J Clin Exp Pathol Source Type: research

Glomerular and tubular C4d depositions in IgA nephropathy: relations with histopathology and with albuminuria.
CONCLUSIONS: Activation of the complement system was involved in renal damage and was identified through deposition of C4d in the glomerulus and tubules. Positive C4d staining in the glomerulus and the tubules may be associated with functional damage related to glomerular filtration and poor renal outcome. PMID: 23638222 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Maeng YI, Kim MK, Park JB, Cho CH, Oh HK, Sung WJ, Park KK Tags: Int J Clin Exp Pathol Source Type: research

Overexpression of IL-9 receptor in diffuse large B-cell lymphoma.
Abstract Interleukin-9 (IL-9) is initially described as a growth factor secreted by helper T cells. It acts on a variety of immune cells via its receptor (IL-9R). Recently, the oncogenic activities of IL-9 and IL-9R were reported in some lymphomas but not diffuse large B-cell lymphoma (DLBCL). The purpose of the present study is to investigate the expression of IL-9R in pathological tissues from patients with DLBCL and to evaluate its correlation with clinical characteristics. Tissue samples from patients with DLBCL and reactive lymphoid hyperplasia were analyzed using RT-PCR, western blot and immunohistochemical ...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Lv X, Feng L, Fang X, Jiang Y, Wang X Tags: Int J Clin Exp Pathol Source Type: research

Intestinal protein expression profile identifies inflammatory bowel disease and predicts relapse.
Abstract To date, most studies have applied individual factors as indicators of disease classification and prognosis. The aim of this study is to determine whether clustering analysis of protein expression profiles in intestinal epithelia improves classification and prognosis in patients with inflammatory bowel disease (IBD). One hundred and twenty Crohn's disease (CD) patients, 117 ulcerative colitis (UC) patients and 120 cases of nonspecific colitis provided intestinal biopsy samples for tissue microarray (TMA). Both unsupervised and supervised analyses were used for evaluation of clustering and association with...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Shen J, Qiao Y, Ran Z, Wang T, Xu J, Feng J Tags: Int J Clin Exp Pathol Source Type: research

Diffuse hyperplastic mesothelial cells in multiple lymph nodes: case report with review of the literature.
We report a case of diffuse hyperplastic mesothelial cells in multiple lymph nodes. Microscopically, the lymph nodes had a normal follicular pattern. The lymphatic sinus was extremely expanded, within the sinuses the epithelial-like cells proliferated actively in the form of sheets and clusters. Epithelioid-like cells had eosinophilic cytoplasm, prominent nucleoli and vesicular nuclei. Mitotic figures were rarely observed. These cells were immunopositive for Calretinin, CK5/6, D2-40, MC and Ckpan and immunonegative for S-100, HMB45, MelanA, TTF-1, CDX-2, Villin, ALK, CD30, CD20, CD3, CD1a and CD68. In addition, during a 22...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Peng L, Shen Q, Liu X, Wang J, Shi S, Yu B, Zhou X Tags: Int J Clin Exp Pathol Source Type: research

Adenoid cystic carcinoma of the oral cavity: immunohistochemical study of four cases.
This report describes the immunohistochemical study of four ACCs of the oral cavity. The male to female ratio was 1:3. The age was 43, 51, 59 and 64 years. The location was tongue (n=2), buccal mucosa (n=1), and gingiva (n=1). Histochemically, all ACCs were positive for neutral, carboxylated and sulfated mucins. Immunohistochemically, ACC was consistently positive for cytokeratin (CK) AE1/3, CK 34βE12, CK5/6, CK7, CK14, CK18, p63, CA19-9, c-KIT (CD117), PDGFRA, MUC1, and Ki-67 (labeling index: 5%, 15%, 20% and 40%). ACC was consistently negative for CK8, CK20, desmin, S100 protein, CD34, chromogranin, MUC2, MUC5AC and...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research

Recurrent multifocal Langerhans cell histiocytosis of the mandible and maxilla in a 46-year-old man: a pathologic case report.
Abstract A 46-year-old man consulted to a private dental clinic for tooth extraction, where he was indicated to have abnormal shadow in the right mandible. The patient was referred to our clinic hospital. X-ray examination revealed an osteolytic lesion (3 x 2 x 1 cm), and tumor excision was performed. Pathological diagnosis was difficult. The tumor consisted of round cells with moderate atypia. Nuclear grooves were recognized. Immunohistochemistry showed positive CD1a and S100 protein. The Ki67 labeling was 16%. The author diagnosed the lesion as Langerhans cell histiocytosis (LCH). The patient became free of tumo...
Source: International Journal of Clinical and Experimental Pathology - May 26, 2013 Category: Pathology Authors: Terada T Tags: Int J Clin Exp Pathol Source Type: research