Directed differentiation of skin-derived precursors into fibroblast-like cells.
Abstract Skin-derived precursors (SKPs), which are located at skin's dermis, display multi-lineage potential and can produce both neural and mesodermal progeny in vitro. SKPs are considered to take part in dermal reconstruction and may be an important source of fibroblast during wound repairing. To explore the possibility of differentiation of SKPs into fibroblasts, the 3(rd) passage SKPs were treated with 0, 20, 40, 100, or 500 ng/ml human recombinant connective tissue growth factor (CTGF) for 48 h or treated with 100 ng/ml CTGF for 0, 24, 48, 72, or 96 h. Subsequently, a series of methods were to be used to obse...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Shu B, Xie JL, Xu YB, Yu JX, Shi Y, Liu J, Wang P, Liu XS, Qi SH Tags: Int J Clin Exp Pathol Source Type: research

Bumetanide protects focal cerebral ischemia-reperfusion injury in rat.
CONCLUSION: These results suggest that bumetanide protects focal cerebral ischemia-reperfusion injury in rat, which might through the inhibition of NKCC1. PMID: 24817944 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Wang G, Huang H, He Y, Ruan L, Huang J Tags: Int J Clin Exp Pathol Source Type: research

Acute and subacute toxicity study of 1,8-cineole in mice.
Abstract The effects of acute and subacute toxicity of 1,8-cineole in Kunming mice were studied. After acute oral administration, the LD50 value (95% CL) was 3849 mg/kg (3488.8~4247.1 mg/kg). In the subacute toxicity study, there were no significant differences in body weight and relative organ weight between the control group and 1,8-cineole treatment groups. The histopathological examinations showed that granular degeneration and vacuolar degeneration appeared in liver and kidney tissue after administration of high dose of 1,8-cineole. Under electron microscopy, a series of ultrastructural changes were observed:...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Xu J, Hu ZQ, Wang C, Yin ZQ, Wei Q, Zhou LJ, Li L, Du YH, Jia RY, Li M, Fan QJ, Liang XX, He CL, Yin LZ Tags: Int J Clin Exp Pathol Source Type: research

Nrf2 induces cisplatin resistance through activation of autophagy in ovarian carcinoma.
CONCLUSIONS: Nrf2-activated autophagy may function as a novel mechanism causing cisplatin-resistance. PMID: 24817946 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Bao LJ, Jaramillo MC, Zhang ZB, Zheng YX, Yao M, Zhang DD, Yi XF Tags: Int J Clin Exp Pathol Source Type: research

DNA microarray reveals ZNF195 and SBF1 are potential biomarkers for gemcitabine sensitivity in head and neck squamous cell carcinoma cell lines.
Abstract Gemcitabine is a potential chemotherapy drug for treatment of head and neck squamous cell carcinoma (HNSCC), however, the poor or partial response of HNSCC patients to gemcitabine demonstrated the urgent need for gemcitabine biomarkers to improve the therapy. In present work, 10 HNSCC cell lines were employed to figure out the biomarkers for gemcitabine sensitivity. The sensitivities of these 10 cell lines to gemcitabine and the basal expression of these cell lines was investigated, the correlation between gemcitabine response (IC50 dose) and gene expression was investigated by Pearson correlation and FDR...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Zhu MH, Ji SL, Zhang CY, Cui L, Xiong L, Zheng HL Tags: Int J Clin Exp Pathol Source Type: research

RNA-seq reveals determinants of sensitivity to chemotherapy drugs in esophageal carcinoma cells.
Abstract Chemotherapy remains the mainstay of treatment for patients with incurable disease of esophageal carcinoma. Most patients respond poorly to chemotherapy, it is necessary to figure out biomarkers for chemotherapy sensitivity or resistance to perform the individualized therapy. In present work, the sensitivities of two ESCC cell lines to 9 chemotherapy drugs were identified and the transcriptome of these two cell lines were investigated by RNA-seq, the correlation between the sensitivity to drugs and expression of some genes was attempted to construct. Eca-1 was more resistant to most of the chemotherapy dr...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Yang LX, Li BL, Liu XH, Yuan Y, Lu CJ, Chen R, Zhao J Tags: Int J Clin Exp Pathol Source Type: research

FOXL2 suppresses proliferation, invasion and promotes apoptosis of cervical cancer cells.
In this study, we measured the expression of FOXL2 in cervical cancer by immunohistochemistry and its mRNA level in cervical cancer cell lines Hela and Siha by RT-PCR. Then we overexpressed FOXL2 in Hela cells and silenced it in Siha cells by plasmid transfection and verified using western blotting. When FOXL2 was overexpressed or silenced, cells proliferation and apoptosis were determined by Brdu assay and Annexin V/PI detection kit, respectively. In addition, we investigated the effects of FOXL2 on the adhesion and invasion of Hela and Siha cells. Finally, we analyzed the influences of FOXL2 on Ki67, PCNA and FasL by flo...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Liu XL, Meng YH, Wang JL, Yang BB, Zhang F, Tang SJ Tags: Int J Clin Exp Pathol Source Type: research

Expression of Hsp90α and cyclin B1 were related to prognosis of esophageal squamous cell carcinoma and keratin pearl formation.
Expression of Hsp90α and cyclin B1 were related to prognosis of esophageal squamous cell carcinoma and keratin pearl formation. Int J Clin Exp Pathol. 2014;7(4):1544-52 Authors: Huang T, Chen S, Han H, Li H, Huang Z, Zhang J, Yin Q, Wang X, Ma X, Dai P, Duan D, Zou F, Chen X Abstract Hsp90α (heat shock protein 90α), one of the important molecular chaperones in cancer cell signal transduction, has been a new candidate target for cancer therapy. Cyclin B1, the client protein of Hsp90α, plays a key role as a mitotic cyclin in the G2-M phase transition during the cell cycle progres...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Huang T, Chen S, Han H, Li H, Huang Z, Zhang J, Yin Q, Wang X, Ma X, Dai P, Duan D, Zou F, Chen X Tags: Int J Clin Exp Pathol Source Type: research

Enhanced serum methylated p16 DNAs is associated with the progression of gastric cancer.
Abstract OBJECTIVE: The present study is to evaluate the effect of methylated p16 on the progression in patients with gastric cancer (GC), and develop a useful biomarker for predicting patient's prognosis. DESIGN AND METHODS: Methylation status of p16 in GC, their corresponding para-cancerous histological normal tissues (PCHNTs), preoperative peritoneal washes (PPWs) and serum were assessed using real-time methylation specific-PCR (MSP). RESULTS: The frequency of p16 methylation was significantly higher in GC tissues (85.9%; 79/92) than that in paired PCHNTs (12.0%; 11/92) (P
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Wu YC, Lv P, Han J, Yu JL, Zhu X, Hong LL, Zhu WY, Yu QM, Wang XB, Li P, Ling ZQ Tags: Int J Clin Exp Pathol Source Type: research

Generation of homologous cell pairs using the oral lymphatic system.
In conclusion, CAL-27, Tca-83, and HeLa cells could be used to simulate the lymphatic metastasis process of oral cancer in vivo. Furthermore, the homologous cell pairs (CAL-27 and LN-CAL-27; HeLa and LN-HeLa) are potential tools for in vitro investigation of the mechanisms underlying metastasis. PMID: 24817952 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Pu YF, Wang L, Wu HH, Bian H, Hong YY, Wang YX, Guo CB Tags: Int J Clin Exp Pathol Source Type: research

Lgr5 over-expression is positively related to the tumor progression and HER2 expression in stage pTNM IV colorectal cancer.
Abstract Recent studies display that Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) appears to involve the initiation of colorectal cancer (CRC). However, its role in the progression of CRC is not clear at present. In the present study, the expression of Lgr5, HER2, VEGF, and Ki-67 was detected by immunohistochemistry in primary cancer tissue and the matched normal mucosa, metastatic lymph node and distant metastatic tissues in 42 CRC cases staged as pTNM IV. The correlation of Lgr5 over-expression with the CRC progression, survival time, and expression of HER2, VEGF, and Ki-67 was evaluated. M...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Gao FJ, Chen JY, Wu HY, Shi J, Chen M, Fan XS, Huang Q Tags: Int J Clin Exp Pathol Source Type: research

Hyperlipidemia compromises homing efficiency of systemically transplanted BMSCs and inhibits bone regeneration.
CONCLUSIONS: Hyperlipidemia compromises homing efficiency of systemically transplanted BMSCs and inhibits bone regeneration. PMID: 24817954 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Xu QC, Hao PJ, Yu XB, Chen SL, Yu MJ, Zhang J, Yang PS Tags: Int J Clin Exp Pathol Source Type: research

Metadherin contribute to BCR signaling in chronic lymphocytic leukemia.
In this study, we further investigate the role of MTDH in regulation of BCR signaling pathway in CLL. Six CLL samples whose cells were proliferation after BCR activation were chosen from patients with unmutated IgVH. CCK-8 method used to evaluate the proliferation rate. MTDH expression was measured by quantitative PCR and Western blot. After BCR activation, there exist upregulation of MTDH expression in mRNA and protein level in all six CLL patients (P
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Li PP, Yao QM, Zhou H, Feng LL, Ge XL, Lv X, Chen N, Lu K, Wang X Tags: Int J Clin Exp Pathol Source Type: research

Increased Tbx1 expression may play a role via TGFβ-Smad2/3 signaling pathway in acute kidney injury induced by gentamicin.
Increased Tbx1 expression may play a role via TGFβ-Smad2/3 signaling pathway in acute kidney injury induced by gentamicin. Int J Clin Exp Pathol. 2014;7(4):1595-605 Authors: Jiang H, Li L, Li-Ling J, Qiu G, Niu Z, Jiang H, Li Y, Huang Y, Sun K Abstract T-box 1 (Tbx1) gene is closely involved in embryonic kidney development. To explore the role of Tbx1 in acute kidney injury (AKI) and the underlying mechanism, we detected the expression of Tbx1 and components of transforming growth factor-beta (TGF-β) signaling pathways including TGF-β, phosphorylated Smad2/3 (p-Smad2/3) and phosphorylat...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Jiang H, Li L, Li-Ling J, Qiu G, Niu Z, Jiang H, Li Y, Huang Y, Sun K Tags: Int J Clin Exp Pathol Source Type: research

Overexpression of GEFT, a Rho family guanine nucleotide exchange factor, predicts poor prognosis in patients with rhabdomyosarcoma.
Abstract Rhabdomyosarcoma (RMS) is one of the most common soft-tissue sarcomas in children and adolescents with poor prognosis. Yet, there is lack of effective prognostic biomarkers for RMS. The present study, therefore, aimed to explore potential biomarkers for RMS based on our previous findings using array comparative genomic hybridization. We investigated guanine nucleotide exchange factor, GEFT, at expression level in 45 RMS patients and 36 normal striated muscle controls using immunohistochemistry using tissue microarrays. The expression rate of GEFT in RMS samples (42/45, 93.33%) was significantly higher (P
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Sun C, Liu C, Li S, Li H, Wang Y, Xie Y, Li B, Cui X, Chen Y, Zhang W, Li F Tags: Int J Clin Exp Pathol Source Type: research

Retrospective analysis of 80 cases with uterine carcinosarcoma, leiomyosarcoma and endometrial stromal sarcoma in China, 1988-2007.
Conclusion. Our retrospective data in part reflect clinical characteristics of uterine sarcoma in China, and form the basis for further concerning researches. PMID: 24817958 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Gao Y, Meng H, Zhang Y, Jiao T, Hui N Tags: Int J Clin Exp Pathol Source Type: research

Expression of stabilin-1 in M2 macrophages in human granulomatous disease and melanocytic lesions.
hmieder A Abstract Stabilin-1 is an endocytotic scavenger receptor, specifically expressed by non-continuous sinusoidal endothelial cells in the liver, spleen and lymph nodes and by M2 or alternatively activated macrophages in human malignancies. We analysed paraffin-embedded tissue of melanocytic lesions and granulomatous diseases for stabilin-1 expression, using the human/murine RS1 antibody. The specificity of the RS1 staining was confirmed in a knockout model, as only M2-like tumor-associated macrophages and vessels of a B16F10 melanoma in wild type mice stained positive; while staining of tumor-associated mac...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Schönhaar K, Schledzewski K, Michel J, Dollt C, Gkaniatsou C, Géraud C, Kzhyshkowska J, Goerdt S, Schmieder A Tags: Int J Clin Exp Pathol Source Type: research

Characterization of the pathologic and endoscopic measurements of colorectal polyp sizes with a focus on sessile serrated adenoma and high-grade dysplasia.
Abstract BACKGROUND: The characteristics of pathologically measured (PMS) and endoscopically measured sizes (EMS) of the colorectal polyps (CRPs) is poorly understood, particularly in polypoid unremarkable mucosa (PUM), sessile serrated adenoma (SSA), and high-grade dysplasia (HGD). METHODS: To characterize the discordance and correlation between the PMS and EMS of CRPs including PUM, SSA, HGD, hyperplastic polyp (HP) and adenomas, we conducted this prospective observational study on the polyps collected between August 2012 and December 2013. RESULTS: PMS was significantly smaller than EMS in the 497 qua...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Li F, Chen Z, Yang Y, Yi X, Yang Y, Zhang L Tags: Int J Clin Exp Pathol Source Type: research

Enhancement of interaction of BSEP and HAX-1 on the canalicular membrane of hepatocytes in a mouse model of cholesterol cholelithiasis.
Abstract We induced gallstones in C57L mice fed with a high cholesterol diet and examined the expression of bile salt export pump (BSEP) on the canalicular membrane of hepatocytes and its relation with PKCα and HAX-1.Twenty-four gallstone-prone C57L mice were randomly assigned to receive a high cholesterol diet or a regular diet. Gallstone formation was recorded. BSEP, PKCα and phospho-PKCα expression was examined by immunoblotting assays. Co-expression of BSEP and HAX-1 was studied by immunofluorescent microscopy and immunoprecipitations. Gallstones were formed in all 12 mice fed with the high c...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Kong J, Liu BB, Wu SD, Wang Y, Jiang QQ, Guo EL Tags: Int J Clin Exp Pathol Source Type: research

Co-expression of CD147 and GLUT-1 indicates radiation resistance and poor prognosis in cervical squamous cell carcinoma.
Abstract The aim of this study was to investigate the association of CD147 and GLUT-1, which play important roles in glycolysis in response to radiotherapy and clinical outcomes in patients with locally advanced cervical squamous cell carcinoma (LACSCC). The records of 132 female patients who received primary radiation therapy to treat LACSCC at FIGO stages IB-IVA were retrospectively reviewed. Forty-seven patients with PFS (progression-free survival) of less than 36 months were regarded as radiation-resistant. Eighty-five patients with PFS longer than 36 months were regarded as radiation-sensitive. Using pretreat...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Huang XQ, Chen X, Xie XX, Zhou Q, Li K, Li S, Shen LF, Su J Tags: Int J Clin Exp Pathol Source Type: research

Applicability of next-generation sequencing to decalcified formalin-fixed and paraffin-embedded chronic myelomonocytic leukaemia samples.
Abstract Decalcified formalin-fixed and paraffin-embedded (dFFPE) bone marrow trephines remain the primary source of gDNA in hematopathological diagnostics. Here, we investigated the applicability of next-generation sequencing (NGS) to dFFPE samples. Chronic myelomonocytic leukaemia (CMML) is a haematopoietic stem cell malignancy delineated by genetic heterogeneity. Recently characteristic mutations have been identified for this entity in a distinct group of genes (TET2, CBL, KRAS). We comparatively investigated DNA extracted from fresh mononuclear cells as well as dFFPE samples from four CMML patients employing a...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Bernard V, Gebauer N, Dinh T, Stegemann J, Feller AC, Merz H Tags: Int J Clin Exp Pathol Source Type: research

NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression.
In this study immunohistochemical expression of NOD1, Receptor-interacting protein 2 (RIP2), Caspase12, human β Defensin1, 2 and 3 (hBD1, 2, 3) was examined in 15 normal controls, 30 cases of oral leukoplakia (OLK) and 60 cases of OSCC. The immunostaining score was assessed by 2 pathologists, respectively. We found that the expression of NOD1, RIP2, Caspase12, hBD1, 2, and 3 decreased along with the progression of OSCC. NOD1 expression was correlated significantly to tumor differentiation, lymph node metastasis, and tumor size. Our results also showed the correlation of hBD2, 3 to lymph node metastasis of OSCC. These ...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Wang X, Jiang W, Duan N, Qian Y, Zhou Q, Ye P, Jiang H, Bai Y, Zhang W, Wang W Tags: Int J Clin Exp Pathol Source Type: research

Estrogen-mimicking isoflavone genistein prevents bone loss in a rat model of obstructive sleep apnea-hypopnea syndrome.
Abstract OBJECTIVE: Intermittent hypoxia was introduced to mimic obstructive sleep apnea-hypopnea syndrome (OSAHS) in rats. Then, bone mass, bone strength and bone turnover were evaluated, and the influence of genistein on bone mass reduction was investigated in these rats. METHODS: OSAHS animal model was established via chronic intermittent hypoxia, and genistein (2.5 mg/kg/day) was used to treat OSAHS rats. The bone mineral density (BMD), bone Histomorphometric indicators, bone biomechanics and expressions of genes related to bone formation and resorption (Runx2, Col I, ALP, Osteocalcin, OPG, RANKL and TRAP...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Song L, Liang X, Zhou Y Tags: Int J Clin Exp Pathol Source Type: research

SKIP expression is correlated with clinical prognosis in patients with bladder cancer.
In this study, Quantitative real-time PCR and immunohistochemistry were performed to detect the expression of SKIP in clinical bladder cancer samples. In addition, the correlation of SKIP expression and clinicopathological features and clinical outcomes were analyzed. The expression levels of SKIP in clinical bladder cancer were much higher than that in paired adjacent noncancerous tissues. High expression of SKIP was closely related with histological grades and the poor prognosis of bladder cancer. Based on our data, we speculated that SKIP may be a potential prognostic marker in bladder cancer. PMID: 24817966 [PubMe...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Wang L, Zhang M, Wu Y, Cheng C, Huang Y, Shi Z, Huang H Tags: Int J Clin Exp Pathol Source Type: research

Dysregulation of MicroRNA biosynthesis enzyme Dicer plays an important role in gastric cancer progression.
Abstract BACKGROUND AND AIM: Dicer is one of the most important components in microRNA biogenesis. Although studies have revealed the aberrantly expression of Dicer in types of cancer, the results were greatly controversial. Here we aimed to study the expression of Dicer in gastric cancer (GC) and further explored the possible roles of Dicer in cancer progression. METHODS: The alteration of Dicer-expression in GC and its clinical significance was retrospectively studied with immunohistochemical analyses on 377 cases of cancer tissues using tissue microarray (TMA). Dicer mRNA and protein levels were also exami...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Zhang J, Zhang XH, Wang CX, Liu B, Fan XS, Wen JJ, Shi QL, Zhou XJ Tags: Int J Clin Exp Pathol Source Type: research

Frequency of activating mutations in FGFR2 exon 7 in bladder tumors from patients with early-onset and regular-onset disease.
Abstract The FGF/FGFR-system plays an important role in embryogenesis, tissue homeostasis and carcinogenesis. Mutational activation of FGFR2 resulting in aberrant FGFR2 signaling activation is known from both hereditary germ line alterations and somatic mutations in various malignancies (e.g. breast, gastric or ovarian cancer). FGFR2 mutations are mainly located within the hinge between Ig-like domains (exon 7), around the 3rd Ig-like domains and within the kinase domain. For bladder cancer only sparse data on FGFR2 mutations are available. Most interestingly a case of early-onset papillary carcinoma of the bladde...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Spiegelberg C, Giedl J, Gaisa NT, Rogler A, Riener MO, Filbeck T, Burger M, Ruemmele P, Hartmann A, Stoehr R Tags: Int J Clin Exp Pathol Source Type: research

Alpha-synuclein immunohistochemistry of gastrointestinal and biliary surgical specimens for diagnosis of Lewy body disease.
Abstract IN LEWY BODY DISEASE, LEWY PATHOLOGY (LP: the accumulation of α-synuclein in neuronal perikarya and processes as Lewy bodies and Lewy neurites and dots, respectively) is observed in the central and peripheral nervous systems. Previous autopsy or biopsy studies of individuals with Lewy body diseases (LBDs) indicated that LP could be observed in the peripheral nerves of the gastrointestinal (GI) systems. The aim of this study is to clarify whether examination of GI and biliary surgical specimens would be useful for diagnosing LBD. We analyzed eight patients diagnosed clinically with LBD and with medic...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Ito S, Takao M, Hatsuta H, Kanemaru K, Arai T, Saito Y, Fukayama M, Murayama S Tags: Int J Clin Exp Pathol Source Type: research

Clinical relevance of tag single nucleotide polymorphisms within the CAT gene in patients with PTSD in the Chongqing Han population.
CONCLUSIONS: This result suggests that six tag SNPs of the CAT gene may not be associated with PTSD, and that CAT gene might not influence the development of PTSD in patients following exposure to a traumatic event, also may be the sample sizes too small to allow a meaningful test. PMID: 24817970 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Duan ZX, Li W, Kang JY, Zhang JY, Chen KJ, Li BC, He M, Wang JM Tags: Int J Clin Exp Pathol Source Type: research

Staphylococcus pseudintermedius for CAMP-test.
Abstract CAMP test reliably detects Listeria monocytogenes (Lm) and Streptococcus agalactiae (group B streptococcus, GBS); it is traditionally performed streaking the tested isolate perpendicularly to Staphylococcus aureus (Sa), provided that reference Sa strains (that produce β-hemolysin) are used. In a zone of β-hemolysin activity, in fact, GBS and Lm form typical arrow-shaped hemolytic areas. While Sa production of the toxin is strain-dependent, however, that of Staphylococcus pseudintermedius (Sp), a pet-owner colonizer and an emerging human pathogen, is constitutive, then observed in all clinical is...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Savini V, Paparella A, Serio A, Marrollo R, Carretto E, Fazii P Tags: Int J Clin Exp Pathol Source Type: research

Primary cutaneous anaplastic large cell lymphoma occurring in an atopic dermatitis patient: a case report with review of the literature with emphasis on their association.
Abstract Although the risk of malignant lymphoma in patients with atopic dermatitis (AD) remains controversial, an increased risk of malignant T-cell lymphoma in patients with AD has been reported. Primary cutaneous anaplastic large cell lymphoma (C-ALCL) is a relatively common distinct clinicopathological entity. However, occurrence of C-ALCL in patients with AD has been rarely reported. Herein, we describe the 5(th) reported case of C-ALCL occurring in a patient with AD and review the clinicopathological features. A 30-year-old Japanese male with a long-standing history of AD presented with a gradually enlarged ...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Ishida M, Hodohara K, Yoshii M, Okuno H, Horinouchi A, Shirakawa A, Harada A, Iwai M, Yoshida K, Kagotani A, Yoshida T, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Diffuse alveolar damage in a patient with Epstein Barr virus (EBV)-positive diffuse large B-cell lymphoma,severely low serum folate, and megaloblastic anemia: a case report of autopsy.
We report a case of a 49-year-old Japanese man, who was admitted to our hospital because of severe dyspnea. He was found to have severely low serum folate and megaloblastic anemia, which was at first suspected to cause his dyspnea. It was assumed that severely low serum folate might be related to his malnutrition, probably caused by habitual alcohol consumption. He died in several days because of acute respiratory distress syndrome. Autopsy revealed diffuse alveolar damage (DAD) in the lungs and systemic lymph node swelling by EBV-positive diffuse large B-cell lymphoma, in addition to megaloblastic anemia in the bone marro...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Matsuda I, Kajimoto N, Hamada M, Shimizu Y, Kuki S, Hirota S Tags: Int J Clin Exp Pathol Source Type: research

Occurrence of Epstein-Barr virus-associated plasmacytic lymphoproliferative disorder after antithymocyte globulin therapy for aplastic anemia: a case report with review of the literature.
Abstract It is well established that patients with immunosuppression have a higher risk of development of lymphoproliferative disorders (LPDs), and Epstein-Barr virus (EBV) is associated with development of LPDs. Aplastic anemia (AA) is an immune-mediated hematological disorder, and immunosuppression therapy (IST), such as antithymocyte globulin (ATG), is widely used for treatment of AA. However, occurrence of LPD without bone marrow transplantation has been extremely rarely documented in patients with IST for AA. Herein, we report the 6(th) documented case of EBV-associated LPD after IST for AA and review the cli...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Nakanishi R, Ishida M, Hodohara K, Okuno H, Yoshii M, Horinouchi A, Shirakawa A, Harada A, Iwai M, Yoshida K, Kagotani A, Yoshida T, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Clear cell adenocarcinoma present exclusively within endometrial polyp: report of two cases.
Abstract Endometrial polyp is a common benign lesion that protrudes into the endometrial surface. The incidence of carcinoma within endometrial polyp is thought to be low, however, postmenopausal women with endometrial polyps are at an increased risk. Endometrial clear cell adenocarcinoma is a distinct and relatively rare subtype of endometrial carcinoma, and recent studies have proposed putative precursor lesions of clear cell adenocarcinoma, namely clear cell endometrial glandular dysplasia (EmGD) and clear cell endometrial intraepithelial carcinoma (EIC). Herein, we describe two cases of clear cell adenocarcino...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Ishida M, Iwai M, Yoshida K, Kagotani A, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

A young man with primary prostatic extra-gastrointestinal stromal tumor: a rare case report and review of the literature.
Abstract Mounting evidence demonstrates the presence of extragastrointestinal stromal tumor (EGIST) which originates from tissues outside the gastrointestinal (GI) tract and shares overlapping immunohistological features with gastrointestinal stromal tumor (GIST). GIST emanating from prostate is extremely rare. To our knowledge, there are only 3 definitely reported cases of primary prostatic EGIST. Herein, we report a case of prostatic EGIST in 31-year-old man with low urinary tract symptoms who was initially misdiagnosed as sarcoma of prostate. Imaging studies assist in determining the origin and location of EGIS...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Zhang ZH, Feng GW, Liu ZF, Qiao L, Zhang T, Gao C, Xu Y Tags: Int J Clin Exp Pathol Source Type: research

Primary neuroendocrine tumor of the testis: a study of clinicopathological features.
This study aimed to explore the clinicopathological characteristics and differential diagnosis of primary neuroendocrine tumor (G1) of the testis. In this paper, we analyze the clinical, histomorphological and immunohistochemical findings, treatment and prognosis of a patient with primary neuroendocrine tumor of the testis, and discuss the relevant literature. A 52-year-old man presented with a painless testicular swelling since 6 months. Histopathological examination showed that the tumor cells were arranged in island and beam patterns. The tumor cells were uniform, polygonal and had moderately eosinophilic cytoplasm and ...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Han X, Yu L, Yang S, Zheng J Tags: Int J Clin Exp Pathol Source Type: research

Sporadic hemangioblastoma of the retroperitoneum.
Abstract Hemangioblastoma is a rare neoplasm occurred in soft tissue. Herein, we present an intriguing case of sporadic hemangioblastoma in the retroperitoneum occurring in a 59-year-old male. The tumor was characterized by an alternation of cellular and paucicellular areas surrounded by a thick fibrous capsule. The stromal cells showed a pale cytoplasm exhibiting occasional lipid droplets and hyperchromatic, irregularly nuclei. CD56, NSE, a-inhibin and S100 were positive in tumor cells. This is the third reported case of a hemangioblastoma in the retroperitoneum. Based on clinical examination, computed tomography...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Huang Y, Han XC, Lv GS Tags: Int J Clin Exp Pathol Source Type: research

Coexistent loss of INI1 and BRG1 expression in a rhabdoid renal cell carcinoma (RCC): implications for a possible role of SWI/SNF complex in the pathogenesis of RCC.
In this study, we analyzed the immunohistochemical and molecular profiles of an unusual RCC showed coexistent absence of INI1 and BRG1 expression, rhabdoid morphology, and poor prognosis. Histologically, the tumor had rhabdoid features, which were demonstrated by large round to polygonal cells with eccentric nuclei, prominent nucleoli, and eosinophilic cytoplasm varying from abundant to scanty. Immunohistochemically, the tumor were positive for BRM, PBRM1, ARID1A, CD10, CKpan, Vimentin, carbonic anhydrase IX (CA-IX), and P504S (AMACR) but negative for INI1, BRG1, HMB45, melan A, CK7, CD117, Ksp-cadherin, TFEB, TFE3, and Ca...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Rao Q, Xia QY, Shen Q, Shi SS, Tu P, Shi QL, Zhou XJ Tags: Int J Clin Exp Pathol Source Type: research

Breast carcinoma with osteoclastic giant cells: case report and review of the literature.
Abstract Breast carcinoma with osteoclastic giant cells (OGCs) are uncommon. Here, we report a 46-year-old woman with a painless lump in her left breast that has been proved clinically and radiographically. Microscopical examination showed OGCs accompanying invasive ductal carcinoma. Immunohistochemical assay revealed that OGCs derived from macrophages. Despite positive lymph node metastasis, the patient has been well without evidence of recurrence or metastasis one year after the operation. To date, the influence of OGCs on the prognosis of patients is still controversial. Our case may provide insights into furth...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Niu Y, Liao X, Li X, Zhao L Tags: Int J Clin Exp Pathol Source Type: research

Small cell type neuroendocrine carcinoma colliding with squamous cell carcinoma at esophagus.
We report here a case of collision tumor at lower third esophagus composed of small cell type neuroendocrine carcinoma (NEC), which is an very rare, highly aggressive and poorly prognostic carcinoma and squamous cell carcinoma (SqCC). In our case, pathologically, the small cell carcinoma display the characteristic of small, round, ovoid or spindle-shaped tumor cells with scant cytoplasm, which colliding with a moderately differentiated squamous cell carcinoma. Immunohistochemical staining demonstrated positive activities for CD56, synaptophysin, 34βE12, CK 5/6, ki-67 (70%-80%), but negative for CD99, chromogranin A, a...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Yang L, Sun X, Zou Y, Meng X Tags: Int J Clin Exp Pathol Source Type: research

Secondary oligodendroglioma after postoperative irradiation for medulloblastoma: a case report and review of the literature.
In this report, we described a patient with secondary oligodendroglioma after postoperative craniospinal irradiation for medulloblastoma. PMID: 24817982 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: He HL, Lee YE, Chen HJ, Hsu CT, Huang YY, Chang IW Tags: Int J Clin Exp Pathol Source Type: research

Therapy-related B lymphoblastic leukemia with t(4;11)(q21;q23)/AF4-MLL in a patient with mantle cell lymphoma after recent aggressive chemotherapy - a unique case report.
Abstract Mantle cell lymphoma (MCL) is a mature B-cell lymphoma associated with the hallmark translocation t(11;14)(q13;32), which involves the cyclin D1 (CCND1) and immunoglobin heavy chain (IgH) genes. It may transform to a more aggressive blastoid or pleomorphic variant, with or without acquisition of chromosomal abnormalities. MCL could also present with a leukemic phase with marked lymphocytosis. A literature search did not reveal any prior reports of MCL transforming to or followed by a B-cell lymphoblastic leukemia (B-ALL). PMID: 24817983 [PubMed - in process] (Source: International Journal of Clinical...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Holdener SL, Harrington L, Nguyen J, Horna P, Sagatys E, Shah B, Zhang L Tags: Int J Clin Exp Pathol Source Type: research

Secondary intestinal amyloidosis presenting intractable hematochezia: a case report and literature review.
Abstract Amyloidosis is characterized by an extracellular deposition of insoluble fibrils. Amyloid deposition caused various clinical symptoms associated with affected organs. Secondary amyloidosis without renal involvement and chronic inflammatory conditions is rarely reported. We experienced a case of secondary intestinal amyloidosis presented with recurrent hematochezia and abdominal pain in a 54-year-old male. Sigmoidoscopy and abdominal computed tomography (CT) presented ischemic colitis and necrosis of whole colon. On microscopically, pinkish amorphous materials were infiltrated in the lamina propria and the...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Kim SH, Kim JH, Gu MJ Tags: Int J Clin Exp Pathol Source Type: research

Adenosquamous carcinoma of the tongue: a case report with review of the literature.
PMID: 24817985 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Ishida M, Iwai M, Kagotani A, Iwamoto N, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Tissue Micro Arrays for immunohistochemical detection of inflammatory infiltrates in renal cell carcinoma.
PMID: 24817986 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Scognamiglio G, Cantile M, Scala S, Cecere S, Russo F, Collina F, Marra L, Sabbatino F, Botti G, Franco R Tags: Int J Clin Exp Pathol Source Type: research

Ectopic respiratory mucosa in the rectum: the second documented case with discussion of its histogenesis.
PMID: 24817987 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Ishida M, Iwai M, Yoshida K, Kagotani A, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Epidermal cyst of the skin with ossification: report of two cases.
PMID: 24817988 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Ishida M, Iwai M, Kagotani A, Iwamoto N, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Genetic alterations in quadruple malignancies of a patient with multiple sclerosis: their role in malignancy development and response to therapy.
We report a female patient with multiple sclerosis and quadruple cancers of different embryological origin. Patient was diagnosed with stage III (T3, N1a, MO) medullary thyroid carcinoma (MTC), multicentric micropapillary thyroid carcinoma, scapular and lumbar melanomas (Clark II, Breslow II), and lobular invasive breast carcinoma (T1a, NO, MO). All tumors present in our patient except micropapillary thyroid carcinomas were investigated for gene alterations known to have a key role in cancer promotion and progression. Tumor samples were screened for the p16 alterations (loss of heterozygosity and homozygous deletions), los...
Source: International Journal of Clinical and Experimental Pathology - May 15, 2014 Category: Pathology Authors: Milosevic Z, Tanic N, Bankovic J, Stankovic T, Buta M, Lavrnic D, Milovanovic Z, Pupic G, Stojkovic S, Milinkovic V, Ito Y, Dzodic R Tags: Int J Clin Exp Pathol Source Type: research

Hyperexpression of NOTCH-1 is found in immature acute myeloid leukemia.
Abstract The Notch signaling pathway is a cell program that is active during early development of multicellular organisms and is required for the formation of basic structures in the growing embryo. Scientific evidence which has accumulated during the last years clearly indicates that aberrant pathway activation may also be critical for the pathogenesis of malignant disease. Despite some limited information the exact role of the Notch signaling pathway in acute myeloid leukemia (AML) remains poorly defined. Immunohistochemical staining of paraffin-embedded bone marrow biopsies from 97 patients with AML, treated be...
Source: International Journal of Clinical and Experimental Pathology - April 7, 2014 Category: Pathology Authors: Sliwa T, Awsa S, Vesely M, Rokitte D, Grossschmidt P, Jilch R, Ulrich W, Geissler K Tags: Int J Clin Exp Pathol Source Type: research

Opposing role of JNK-p38 kinase and ERK1/2 in hydrogen peroxide-induced oxidative damage of human trophoblast-like JEG-3 cells.
Abstract Trophoblasts play a crucial role in embryo implantation and maintenance of normal pregnancy. Recently, oxidative stress has been considered as one important factor in the pathogenesis of spontaneous abortion and preeclampsia. Many studies have reported that the plasma levels of hydrogen peroxide (H2O2) are significantly increased in women with preeclampsia, but the mechanisms involved in H2O2-induced cell cytotoxicity in trophoblasts are still not completely explained. Our present study was undertaken to provide a united understanding of the role of oxidative stress generated by H2O2 on human trophoblasts...
Source: International Journal of Clinical and Experimental Pathology - April 7, 2014 Category: Pathology Authors: Tang C, Liang J, Qian J, Jin L, Du M, Li M, Li D Tags: Int J Clin Exp Pathol Source Type: research

Prognostic significance of Flotillin1 expression in clinically N0 tongue squamous cell cancer.
CONCLUSION: Our results suggest that overexpression of FLOT1 can be found in patients with higher pathological stage, T classification, N classification or recurrence. FLOT1 expression is associated with cN0 TSCC progression and may be valuable for the prognostic evaluation of cN0 TSCC. PMID: 24695539 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - April 7, 2014 Category: Pathology Authors: Li H, Zhang Y, Chen SW, Li FJ, Zhuang SM, Wang LP, Zhang J, Song M Tags: Int J Clin Exp Pathol Source Type: research