TLR2-deficiency of cKit(+) bone marrow cells is associated with augmented potency to stimulate angiogenic processes.
CONCLUSIONS: The absence of TLR2 on cKit(+) BMC is associated with augmented potency to support angiogenic processes in vitro and in vivo. Functional inhibition of TLR2 may therefore provide a novel tool to enhance stem cell function for the treatment of vascular diseases. PMID: 24294367 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Wagner NM, Bierhansl L, Butschkau A, Noeldge-Schomburg G, Roesner JP, Vollmar B Tags: Int J Clin Exp Pathol Source Type: research

MiR-195 inhibits proliferation and growth and induces apoptosis of endometrial stromal cells by targeting FKN.
Abstract MiR-195, which exhibits a proliferation-inhibiting role in different tumors, has been reported to be down-regulated in the ectopic endometrium. The aim of this study was to determine the impact of miR-195 on the biological characteristic of the endometrial stromal cells (ESCs). MiR-195 has been presumed to target the 3'-untranslated regions (3'-UTR) of Fractalkine (FKN), which also plays important roles in endometriosis. Fluorescence reporter assays showed that miR-195 effectively binds to the 3'-UTR of FKN. The normal ESCs showed a significant higher miR-195 expression than that of eutopic and ectopic ES...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Wang Y, Chen H, Fu Y, Ai A, Xue S, Lyu Q, Kuang Y Tags: Int J Clin Exp Pathol Source Type: research

Cancer testis antigen OY-TES-1 expression and serum immunogenicity in colorectal cancer: its relationship to clinicopathological parameters.
In this study, OY-TES-1 polyclonal antibody was generated. The expression of OY-TES-1 mRNA and protein was detected by RT-PCR and immunohistochemistry in 60 CRC and paired adjacent non-tumor tissues, 24 colorectal adenoma and 3 normal colon tissues, respectively. Sera from 73 CRC patients were also tested for OY-TES-1 antibody by ELISA. Our results showed that the frequency of OY-TES-1 mRNA expression was statistically higher in CRC (73.3%, 44/60) than that in adjacent non-tumor tissue (55.0%, 33/60) and colorectal adenoma (45.8%, 11/24). For the first time, OY-TES-1 protein expression was found in (43.3%, 26/60) of CRC ti...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Luo B, Yun X, Fan R, Lin YD, He SJ, Zhang QM, Mo FR, Chen F, Xiao SW, Xie XX Tags: Int J Clin Exp Pathol Source Type: research

Elevated expression of SOX2 and FGFR1 in correlation with poor prognosis in patients with small cell lung cancer.
Abstract Objectives: The central issue in this study is to investigate the expression of Sex determining region Y-BOX2 (SOX2) and fibroblast growth factor receptor 1 (FGFR1), evaluate their clinicopathological variables and prognostic significance in small cell lung cancer (SCLC). Methods: Specimens from 222 SCLC patients and 53 adjacent normal lung tissues were detected by the immunohistochemistry for SOX2 and FGFR1 expression. The relationship between the expression of both markers and survival status was determined. Results: Overexpression of SOX2 and FGFR1 were revealed in SCLC tumors than in normal tissues (P
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Yang F, Gao Y, Geng J, Qu D, Han Q, Qi J, Chen G Tags: Int J Clin Exp Pathol Source Type: research

Claudins 1, 2, 3, 4, 5 and 7 in solar keratosis and squamocellular carcinoma of the skin.
Abstract Claudins are tight junction proteins regulating the paracellular permeability of cell layers. We investigated the expression of claudins 1, 2, 3, 4, 5 and 7 in a sample set consisting of a total of 93 cases representing normal skin, actinic keratoses and squamous cell carcinomas of the skin. There were several changes found in claudin expression. Claudin 1 appeared to be progressively decreased in solar keratosis and skin squamous cell carcinomas compared to normal skin while expression of claudin 2 was increased. With claudins 3 and 5 occasional immunoreactivity was found in squamous cell carcinomas. Cla...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Hintsala HR, Siponen M, Haapasaari KM, Karihtala P, Soini Y Tags: Int J Clin Exp Pathol Source Type: research

Mouse p63 variants and chondrogenesis.
Abstract As a critical member of the p53 family of transcription factors, p63 has been implicated a role in development than in tumor formation, because p63 is seldom mutated in human cancers, while p63 null mice exhibit severe developmental abnormalities without increasing cancer susceptibility. Notably, besides the major epithelial and cardiac defect, p63 deficient mice show severe limb and craniofacial abnormalities. In addition, humans with p63 mutations also show severe limb and digit defects, suggesting a putative role of p63 in skeletal development. There are eight p63 variants which encode for the TAp63 an...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Gu J, Lu Y, Qiao L, Ran D, Li N, Cao H, Gao Y, Zheng Q Tags: Int J Clin Exp Pathol Source Type: research

Delineation of biological and molecular mechanisms underlying the diverse anticancer activities of mycophenolic acid.
CONCLUSIONS: These results suggest that MPA has beneficial activities for anticancer therapy through diverse molecular pathways and biological processes. PMID: 24294374 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Dun B, Xu H, Sharma A, Liu H, Yu H, Yi B, Liu X, He M, Zeng L, She JX Tags: Int J Clin Exp Pathol Source Type: research

Increased ZO-1 expression predicts valuable prognosis in non-small cell lung cancer.
Abstract OBJECTIVE: The aim of this study was to investigate the expression of Zonula Occludens-1 (ZO-1) and its potential value as prognostic indicator of survival in patients with primary non-small cell lung cancer (NSCLC). METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry with tissue microarrays were used to characterize the expression of the ZO-1 mRNA and protein in NSCLC. The correlation of ZO-1 expression with clinical characteristics and prognosis was determined by statistical analysis. RESULTS: The ZO-1 mRNA and protein levels were significantly lower in...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Ni S, Xu L, Huang J, Feng J, Zhu H, Wang G, Wang X Tags: Int J Clin Exp Pathol Source Type: research

Bee venom ameliorates compound 48/80-induced atopic dermatitis-related symptoms.
Abstract UNLABELLED: Honeybee (Apis mellifera L.) venom (BV) has been traditionally used for the treatment of pain and inflammatory diseases such as itchy skin problems. However, the precise mechanism of BV in ameliorating the scratching behavior is not fully understood. OBJECTIVE: In order to evaluate the effect of BV on atopic dermatitis-related symptoms in mice, we used a mouse skin scratching model induced by compound 48/80. The anti-itch effect of BV was investigated in a compound 48/80-induced mouse scratching behavior model. BALB/c mice were injected intraperitoneally with vehicle (saline 0.9%) or BV (...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Kim KH, Lee WR, An HJ, Kim JY, Chung H, Han SM, Lee ML, Lee KG, Pak SC, Park KK Tags: Int J Clin Exp Pathol Source Type: research

Decreased expression of miR-218 is associated with poor prognosis in patients with colorectal cancer.
Abstract The identification of biomarkers in colorectal cancer (CRC) diagnosis and therapy is important in achieving early cancer diagnosis and improving patient outcomes. The aim of this study was to examine clinical significance of miR-218 expression in sera and tissues from CRC patients. A total of 189 cases and 30 healthy subjects were included. The expression levels of miR-218, SLIT2 and SLIT3 were measured by quantitative reverse transcription PCR (qRT-PCR). The relationship between miR-218 expression and clinicopathological characteristics was investigated. The expression levels of miR-218, SLIT2 and SLIT3 ...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Yu H, Gao G, Jiang L, Guo L, Lin M, Jiao X, Jia W, Huang J Tags: Int J Clin Exp Pathol Source Type: research

HIF-1α and VEGF expression correlates with thrombus remodeling in cases of intravascular papillary endothelial hyperplasia.
HIF-1α and VEGF expression correlates with thrombus remodeling in cases of intravascular papillary endothelial hyperplasia. Int J Clin Exp Pathol. 2013;6(12):2912-8 Authors: Kim S, Jun JH, Kim J, Kim do W, Jang YH, Lee WJ, Chung HY, Lee SJ Abstract Intravascular papillary endothelial hyperplasia (IPEH) is histopathologically characterized by endothelium-lined papillary structures encircling an acellular fibrin core. The process of IPEH pathogenesis is unclear. The purpose of our study was to identify histopathological and immunohistochemical characteristics of IPEH to better understand the pathogenes...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Kim S, Jun JH, Kim J, Kim do W, Jang YH, Lee WJ, Chung HY, Lee SJ Tags: Int J Clin Exp Pathol Source Type: research

Immunohistochemical expression of nuclear and cytoplasmic survivin in gastrointestinal carcinoma.
Abstract Survivin is a protein that is highly expressed in many embryonic tissues, as well as most human tumors. Prior studies have reported both positive and negative correlations between survivin expression and cancer prognosis, but these associations remain controversial. In the present study, we assessed the expression of nuclear and cytoplasmic survivin in gastrointestinal carcinomas. Using these data, we determined the correlation between nuclear and cytoplasmic survivin and, further, investigated correlations between survivin expression and clinicopathological parameters. Seventy-two advanced gastric adenoc...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Shintani M, Sangawa A, Yamao N, Kamoshida S Tags: Int J Clin Exp Pathol Source Type: research

Clinical usefulness of immunohistochemistry for plakoglobin, N-cadherin, and connexin-43 in the diagnosis of arrhythmogenic right ventricular cardiomyopathy.
Abstract Diagnosing arrhythmogenic right ventricular cardiomyopathy (ARVC) is often challenging because no single diagnostic tool is available to detect the disease. We evaluated whether analysis of plakoglobin, N-cadherin, and connexin-43 immunoreactivity can be used as a significant test in diagnosis of ARVC. We selected subjects with suspicion of ARVC (n=22) in patients who underwent endomyocardial biopsy (EMB) in Kyungpook National University Hospital (n=1326). The patients (n=22) were classified into definite ARVC patients (n=17) and borderline ARVC (n=5). We selected control subjects (n=20) who were autopsie...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Kwon YS, Park TI, Cho Y, Bae MH, Kim S Tags: Int J Clin Exp Pathol Source Type: research

Clear cell papillary renal cell carcinoma: a clinicopathological study emphasizing ultrastructural features and cytogenetic heterogeneity.
In conclusion, the coexpression of CA9 and HIF-1α in the absence of VHL gene abnormalities in CCPRCC suggests activation of the HIF pathway by mechanisms independent of VHL gene mutation. Losses of chromosomes 3 (monosomies chromosome 3) was detected in 3 cases suggesting that at least some of these lesions have demonstrated abnormalities of chromosomes 3. Ultrastructurally, CCPRCC composed of numerous glycogens with scanty cell organelles, reminiscent of CCRCC suggesting the close pathogenesis relationship of CCPRCC with CCRCC. PMID: 24294381 [PubMed - in process] (Source: International Journal of Clinical and ...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Shi SS, Shen Q, Xia QY, Tu P, Shi QL, Zhou XJ, Rao Q Tags: Int J Clin Exp Pathol Source Type: research

Transposable hypomethylation is associated with metastatic capacity of primary melanomas.
In this study, we demonstrate the strong influence of global DNA demethylation in the metastatic formation of primary melanomas during the follow-up period. PMID: 24294382 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Ecsedi SI, Hernandez-Vargas H, Lima SC, Herceg Z, Adany R, Balazs M Tags: Int J Clin Exp Pathol Source Type: research

Leiomyomatosis peritonealis disseminata coexisting with endometriosis within the same lesions: a case report with review of the literature.
Abstract Leiomyomatosis peritonealis disseminata (LPD) is an extremely rare condition, which is characterized by the presence of multiple peritoneal and subperitoneal nodules composed of bland smooth muscle cells. Albeit extremely rare, coexistence of endometriosis within LPD lesions has also reported. Herein, we report the seventh documented case of LPD coexisting with endometriosis within the same lesions and review the pathogenesis of this lesion. A 42-year-old Japanese female presented with an abdominal tumor. Computed tomography revealed a tumorous lesion in the right ovary and multiple small nodules in the a...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Toriyama A, Ishida M, Amano T, Nakagawa T, Kaku S, Iwai M, Yoshida K, Kagotani A, Takahashi K, Murakami T, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Role of calretinin immunohistochemical stain in evaluation of Hirschsprung disease: an institutional experience.
CONCLUSION: The pattern of expression of calretinin in rectal suction biopsies in HD and normal rectum coincide with the ones previously described in the literature. Calretinin IHC offered additional diagnostic value in the specimens with inadequate amount of submucosa and rarely seen ganglion cells. The pattern of expression of calretinin in HD pull-through specimens correlates with the rectal biopsy ones. Faint positivity of the thick submucosal and subserosal nerves in the absence of ganglion cells and calretinin positive nerve fibrils, is characteristic of the junction of the aganglionic-to-normal rectum. We are the fi...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Alexandrescu S, Rosenberg H, Tatevian N Tags: Int J Clin Exp Pathol Source Type: research

Clinical usefulness of fine needle aspiration cytology in patients less than 20 years old: a 10-year experience at a single institution.
Abstract The purpose of this study was to identify the spectrum of cytological diagnoses and evaluate the diagnostic effectiveness of fine needle aspiration cytology (FNA) in patients less than 20 years old. The subjects were selected by retrospectively reviewing records from 1999 to 2009. Selected patients less than 20 years old underwent FNA. Cytological and histological slides of samples from the subjects were reviewed. Our study included a total of 909 subjects with a mean age of 14.6 years. The majority of the FNA samples were taken from lymph nodes (n = 448, 49.3%), with the remaining aspirates obtained from...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Kim S, Jang EJ, Jeong JY, Park JY Tags: Int J Clin Exp Pathol Source Type: research

Association between PKA gene polymorphism and NTDs in high risk Chinese population in Shanxi.
CONCLUSIONS: The gene polymorphism loci rs12132032 in PRKACB maybe a potential risk factor for anencephaly in Chinese population from Shanxi, while gender susceptibility may influence the correlation. PMID: 24294386 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Wu J, Lu X, Wang Z, Shangguan S, Chang S, Li R, Wu L, Bao Y, Niu B, Wang L, Zhang T Tags: Int J Clin Exp Pathol Source Type: research

A new technique for the obliterative urethral strictures.
In this study, we reported single-hospital institution case-series including 97 patients with obliterative urethral strictures were enrolled with "three lines lie within one imaginary plane (TLLWOIP)" to treat with patients with the obliterative urethral strictures. Perioperative variables and success rates were evaluated. Urinary flow rate, residual urine (RU) volume and quality-of-life score (QoLs) of patients were analyzed. In the obliterative urethral strictures, postoperative maximum urinary flow rate was 24.36±10.69 ml, and postoperative RU volume and QoLs outcomes were significantly lower than preop...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Chen X, Wang K, Ye Y, Hao Z, Zhou J, Fan S, Shi H, Liang C Tags: Int J Clin Exp Pathol Source Type: research

Diffuse large B-cell lymphoma of the uterus suspected of having transformed from a marginal zone B-cell lymphoma harboring trisomy 18: a case report and review of the literature.
Abstract The patient was a 72-year-old female with the chief complaint of abdominal fullness. A giant primary myoma of the uterine cervix was suspected, and total hysterectomy was performed. Based on a postoperative histopathological examination of the tumor a diagnosis of diffuse large B-cell lymphoma (DLBCL) was made in the uterus and a mass in the greater omentum was diagnosed as a marginal zone B-cell lymphoma (MZBCL). No flow-cytometry studies or chromosome or gene examinations were performed on a fresh specimen. The results of an examination of a paraffin block histopathology specimen by fluorescence in-situ...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Sugimoto KJ, Imai H, Shimada A, Wakabayashi M, Sekiguchi Y, Nakamura N, Sawada T, Izumi H, Ota Y, Komatsu N, Noguchi M Tags: Int J Clin Exp Pathol Source Type: research

Long segmental hyperplasia of interstitial cells of Cajal with giant diverticulum formation.
We describe herein one such case, forming a giant diverticulum. A 63-year-old woman with no features of Neurofibromatosis 1 (NF1) presented with increasing abdominal pain for more than 30 years. A large, diverticulum-like mass in the ileum was resected. Microscopically, a diffuse proliferation of bland spindle cells was seen extending for 12 cm, replacing the muscularis propria and lined by intact mucosa. The spindle cells were CD117+/CD34+/DOG1+/SMA+/Desmin-/S100-. Mutation analyses did not reveal any mutations in c-KIT or PDGFRA. The lesion had two silent mutations in the NF1 gene. It is rare of the diffuse form of spora...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Xue L, Qiu T, Song Y, Shan L, Liu X, Guo L, Ying J, Zou S, Shi S, Polydorides AD, Zhao X, Lu N, Lin D Tags: Int J Clin Exp Pathol Source Type: research

Endometrial stromal sarcoma arising in vaginaEndometrial stromal sarcoma arising in vagina.
Abstract Endometrial stromal sarcoma (ESS) arising in the vagina is an extremely rare extrauterine endometrial stroma sarcoma, with only 4 cases reported in the literature up to date. Here we report a case of neoplasm originating from vagina. A 32-year-old woman complained of intermittent vaginal bleeding especially after intercourse. A mass with a diameter of 1.0 cm was found in the middle and upper segments of the right posterior vaginal wall. Biopsy showed ESS. Total abdominal hysterectomy, unilateral salpingo-oophorectomy (right) and partial vaginectomy were performed. No ESS lesion was found in endometrium. T...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Liu Z, Ding J, Li X, Yu K Tags: Int J Clin Exp Pathol Source Type: research

Soft tissue perineurioma and other unusual tumors in a patient with neurofibromatosis type 1.
In conclusion, perineurioma may rarely be NF-1 related and should be included in the spectrum of neoplasms occurring in this disorder. PMID: 24294391 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Schaefer IM, Ströbel P, Thiha A, Sohns JM, Mühlfeld C, Küffer S, Felmerer G, Stepniewski A, Pauli S, Agaimy A Tags: Int J Clin Exp Pathol Source Type: research

Post cardiac transplantation T-cell lymphoproliferative disorder presenting as a solitary lung nodule.
We report a case of a 66-year-old man who received an orthotopic heart transplant for cardiomyopathy 7 years prior to presentation. He was referred to our institution with a hypermetabolic solitary right lower lobe lung nodule with an SUV of 9.2 on PET scan. The combined histomorphological and immunohistochemical pattern was most consistent with monomorphic PTLD, peripheral T-cell lymphoma with angioimmunoblastic features. Molecular studies showed clonal T-cell gamma receptor gene rearrangement. Primary pulmonary involvement of T-cell PTLD is extremely rare. This is the third reported case of T-cell PTLD after cardiac tran...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Aqil B, Krishnan B, Curry CV, Elghetany MT, Szigeti R Tags: Int J Clin Exp Pathol Source Type: research

Adult T-cell leukemia/lymphoma accompanying follicular mucinosis: a case report with review of the literature.
Abstract Follicular mucinosis is recognized as one of the histopathological reaction patterns characterized by the accumulation of mucin within follicular epithelium. It is induced by various causes including inflammatory diseases, and more than half of the cases are associated with malignant lymphoma, mainly mycosis fungoides. Herein, we describe the third documented case of adult T-cell leukemia/lymphoma (ATLL) accompanying follicular mucinosis. A 72-year-old Japanese male presented with persistent erythema in his arm and neck. Laboratory tests demonstrated positivity for human T-cell leukemia virus (HTLV)-1 ant...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Ishida M, Iwai M, Yoshida K, Kagotani A, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Follicular hybrid cyst: a combination of bullous pilomatricoma and epidermoid cyst.
Abstract The follicular hybrid is composed of more than two components of pilosebaceous unit. There are several studies of hybrid cyst, combination of trichilemmal and epidermoid cyst was the most frequently reported. In this paper, we reported one case of hybrid cyst composed of bullous pilomatricoma and epidermoid cyst. A 14-year-old girl was complaint of a solitary flesh-colored to erythematous nodule with flaccid appearance sized 3.2×1.8 cm in diameter on her right upper back for one year. The histologic findings showed there were edema and proliferation of capillaries in the superficial dermis, a cyst i...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Sanusi T, Qu X, Li Y, Zhang J, Wang M, Zhao Y, Yang Z, An X, Qian Y, Wang C, Chen H, Chen S, Huang C Tags: Int J Clin Exp Pathol Source Type: research

CD56 positive diffuse large B-cell lymphoma: a case report and literature review.
Abstract CD56 positive B-cell lymphoma is very rare. We experienced a case of CD56 positive diffuse large B-cell lymphoma, occurred in a young child. A 5-year-old girl complained with snoring and open mouth breathing. No any abnormality in laboratory or physical examination was present, except enlarged both tonsils. Bilateral tonsillectomy was performed. Cut sections of right tonsil showed a 2 cm size, solid mass. On microscopically, large monomorphic lymphoid cells were diffusely proliferated and showed positivity for CD20 and CD56 and negative for Epstein-Barr virus (EBV) polymerase chain reaction (PCR). Monoclo...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Gu MJ, Ha JO Tags: Int J Clin Exp Pathol Source Type: research

Esophageal squamous cell carcinoma in situ overlying leiomyoma: a case report with review of the literature.
PMID: 24294396 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Ishida M, Mochizuki Y, Iwai M, Yoshida K, Kagotani A, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Sebaceous carcinoma associated with Bowen's disease: a case report with emphasis on the pathogenesis of sebaceous carcinoma.
PMID: 24294397 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Ishida M, Iwai M, Yoshida K, Kagotani A, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Pigmented porocarcinoma: a case report with review of the literature.
PMID: 24294398 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Ishida M, Iwai M, Yoshida K, Kagotani A, Okabe H Tags: Int J Clin Exp Pathol Source Type: research

Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer.
CONCLUSION: Our results thus demonstrate that spondin 1 is a useful marker for ovarian cancer; additionally, the high percentages of tumors that are positive for spondin 1 make it an ideal target for therapy. CRABP-1 was not expressed at high levels in any subtype of ovarian cancer, making it a poor marker. PMID: 24294399 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Jiao TT, Zhang YM, Yao L, Gao Y, Sun J, Zou DF, Wu GP, Wang D, Ou J, Hui N Tags: Int J Clin Exp Pathol Source Type: research

Development of a model system to analyze chondrogenic differentiation of mesenchymal stem cells.
Abstract High-density cell culture is widely used for the analysis of cartilage development of human mesenchymal stem cells (HMSCs) in vitro. Several cell culture systems, as micromass, pellet culture and alginate culture, are applied by groups in the field to induce chondrogenic differentiation of HMSCs. A draw back of all model systems is the high amount of cells necessary for the experiments. Further, handling of large experimental approaches is difficult due to culturing e.g. in 15 ml tubes. Therefore, we aimed to develop a new model system based on "hanging drop" cultures using 10 to 100 fold less c...
Source: International Journal of Clinical and Experimental Pathology - December 5, 2013 Category: Pathology Authors: Ruedel A, Hofmeister S, Bosserhoff AK Tags: Int J Clin Exp Pathol Source Type: research

The SYT-SSX fusion protein and histological epithelial differentiation in synovial sarcoma: relationship with extracellular matrix remodeling.
Abstract Synovial sarcoma (SS) tumor cells, which have the chromosomal translocation t(X;18)(p11.2;q11.2), have an inherently greater propensity for epithelial differentiation than other mesenchymal tumors, especially spindle cell sarcomas. This is caused by de-repression of the transcription of E-cadherin by SYT-SSX1 and SYT-SSX2, which dissociate Snail or Slug, respectively, from the E-cadherin promoter. However, a subset of SS with SYT-SSX1 loses E-cadherin expression despite adequate de-repression because of mutations in E-cadherin, resulting in monophasic histology. The ratio of the expression levels of SYT-S...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Saito T Tags: Int J Clin Exp Pathol Source Type: research

The effect of porcine ADM to improve the burn wound healing.
Abstract To study the effect of porcine acellular dermal matrix (ADM) on the burn wound healing. Seventy healthy Wistar rats were inflicted with 2 cm second degree burn and divided into 2 groups; one group was treated with porcine ADM and the other with Povidone Iodine Cream. Biopsies were taken on day 1, 3, 5, 7, 10, 14, 21 for histopathological and biochemical analysis to test PCNA, K19, Integrin-β1, PDGF, EGF and FGF. The results revealed relatively better and faster regeneration after treatment of porcine ADM, along with greatly increased synthesis in collagen in the experimental group. PCNA, K19, Integri...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Chen X, Shi Y, Shu B, Xie X, Yang R, Zhang L, Ruan S, Lin Y, Lin Z, Shen R, Zhang F, Feng X, Xie J Tags: Int J Clin Exp Pathol Source Type: research

New bone formation in a bone defect associated to dental implant using absorbable or non-absorbable membrane in a dog model.
a-Barbosa J Abstract The aim of this research was to determine the bone formation capacity in fenestration defects associated with dental implants using absorbable and non-absorbable membranes. Six dogs were used in the study. In both tibias of each animal 3 implants were installed, and around these 5 mm circular defects were created. The defects were covered with absorbable membranes (experimental group 1), non-absorbable membranes (experimental group 2), and the third defect was not covered (control group). At 3 and 8 weeks post-surgery, the animals were euthanized and the membranes with the bone tissue around t...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Lopez Mde A, Olate S, Lanata-Flores A, Pozzer L, Cavalieri-Pereira L, Cantín M, Vásquez B, de Albergaria-Barbosa J Tags: Int J Clin Exp Pathol Source Type: research

Expression and clinical significance of the nin one binding protein and p38 MAPK in prostate carcinoma.
CONCLUSIONS: NOB1 expression status was closely correlated with important histopathologic characteristics and the recurrence and metastasis of prostate carcinomas. These data support a potential link between NOB1 and p38 MAPK, and suggest that NOB1 may identify a subset of prostate cancer patients with a poor prognosis. This study proved that NOB1 in PCa tissue can be used, in combination with traditional clinicopathological factors, as promising diagnostic and prognostic tools. PMID: 24228091 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Che JP, Li W, Yan Y, Liu M, Wang GC, Li QY, Yang B, Yao XD, Zheng JH Tags: Int J Clin Exp Pathol Source Type: research

Influences of HIF-lα on Bax/Bcl-2 and VEGF expressions in rats with spinal cord injury.
In conclusion, administration of Ad-HIF-1α after SCI could ameliorate neuronal apoptosis and promote angiogenesis in rats. Our study provides a basis for further exploration of the relationship between HIF1α and SCI. PMID: 24228092 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Chen MH, Ren QX, Yang WF, Chen XL, Lu C, Sun J Tags: Int J Clin Exp Pathol Source Type: research

Suppressing the malignant phenotypes of glioma cells by lentiviral delivery of small hairpin RNA targeting hypoxia-inducible factor-1α.
Suppressing the malignant phenotypes of glioma cells by lentiviral delivery of small hairpin RNA targeting hypoxia-inducible factor-1α. Int J Clin Exp Pathol. 2013;6(11):2323-32 Authors: Dong CG, Wu WK, Feng SY, Yu J, Shao JF, He GM Abstract Hypoxic microenvironment of solid tumors is known to shape malignant phenotypes of cancer cells through the dimeric transcription factor hypoxia-inducible factor (HIF)-1. In the present study, the therapeutic effect of targeting α subunit of HIF-1 in glioma cells via lentiviral delivery of small hairpin RNA (shRNA) was evaluated. Data from quantitative real...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Dong CG, Wu WK, Feng SY, Yu J, Shao JF, He GM Tags: Int J Clin Exp Pathol Source Type: research

Effects of low-frequency noise on cardiac collagen and cardiomyocyte ultrastructure: an immunohistochemical and electron microscopy study.
Abstract INTRODUCTION: Low-frequency noise (LFN) leads to the development of tissue fibrosis. We previously reported the development of myocardial and perivascular fibrosis and a reduction of cardiac connexin43 in rats, but data is lacking concerning the affected type of collagen as well as the ultrastructural myocardial modifications. OBJECTIVES: The aim of this study was to quantify cardiac collagens I and III and to evaluate myocardial ultrastructural changes in Wistar rats exposed to LFN. METHODS: Two groups of rats were considered: A LFN-exposed group with 8 rats continuously submitted to LFN during...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Antunes E, Borrecho G, Oliveira P, Alves de Matos AP, Brito J, Aguas A, Martins Dos Santos J Tags: Int J Clin Exp Pathol Source Type: research

Protective effect of Curcumin on chemotherapy-induced intestinal dysfunction.
This study aimed to investigate the protective effect of Curcumin on chemotherapy-induced intestinal dysfunction in rats. METHODS: Sixty healthy Wistar rats were randomly divided into control group (normal saline), 5-FU group and 5-FU+Curcumin group. The weight, serum level of endotoxin, DAO and D-lactate were determined. The pathological change of intestinal mucosa structure was studied under light microscopy and electron microscopy. The expression of Bax, Bcl-2 and Caspase-3 were assessed by immunohistochemical staining. RESULTS: The Curcumin intragastrically administrated obviously reduced 5-FU-induced weight-...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Yao Q, Ye X, Wang L, Gu J, Fu T, Wang Y, Lai Y, Wang Y, Wang X, Jin H, Guo Y Tags: Int J Clin Exp Pathol Source Type: research

Spermatocytic seminoma: a 21 years' retrospective study in a tertiary care hospital in Pakistan.
CONCLUSION: CD 117 is of limited utility in differentiating the spermatocytic seminoma from classic seminoma as it is expressed in significant number of spermatocytic seminomas. However, different histological features, PAS special stain and PLAP immunostain are significantly helpful in distinguishing these two entities. PMID: 24228096 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Haroon S, Tariq MU, Fatima S, Kayani N Tags: Int J Clin Exp Pathol Source Type: research

SIRT1 is highly expressed in brain metastasis tissues of non-small cell lung cancer (NSCLC) and in positive regulation of NSCLC cell migration.
Abstract Brain metastases are a frequent and ongoing major complication of non-small cell lung cancer (NSCLC). To deepen our understanding to the underlying mechanisms by which NSCLC cells metastasize to brain and hence to improve the therapy, a high throughput RNAi screening with shRNA library of 153 epigenetic genes was subjected to A549, a NSCLC cell line with high migration ability, to examine the effects of these genes on cell migration by wound-healing assay. The screening results showed that knockdown of 2 genes (KDM5B and SIRT1) dramatically and specifically inhibits A549 migration but not affects the prol...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Han L, Liang XH, Chen LX, Bao SM, Yan ZQ Tags: Int J Clin Exp Pathol Source Type: research

Administration of dexamethasone protects mice against ischemia/reperfusion induced renal injury by suppressing PI3K/AKT signaling.
Abstract Dexamethasone (DEX), a ligand for glucocorticoid receptor (GR), has long been used in the clinical practice due to its anti-inflammatory and immunosuppressive properties. Given that ischemia/reperfusion (IR)-induced renal injury is featured by the excessive immune response; the current study is therefore designed to address the impact of dexamethasone on IR-induced renal injury, a common disorder in the clinical settings. Precondition of mice with 4 mg/kg of dexamethasone significantly attenuated IR-induced injury as manifested by the improved renal function along with ameliorated pathological changes and...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Zhang J, Yao Y, Xiao F, Lan X, Yu C, Zhang Y, Jiang C, Yang J, Pei G, Li Y, Rong S, Hu S, Li J, Xu G Tags: Int J Clin Exp Pathol Source Type: research

FasL on human nucleus pulposus cells prevents angiogenesis in the disc by inducing Fas-mediated apoptosis of vascular endothelial cells.
Abstract The intervertebral disc is the largest avascular organ in the human body. However, with the progress of intervertebral disc degeneration (IDD), the disc tends to be vascularized increasingly via angiogenesis. It is well established that both human nucleus pulposus (NP) cells and vascular endothelial cells express FasL and Fas. However, the issue remains open as to whether there are certain active mechanisms preventing angiogenesis in the disc via the FasL-Fas machinery. Here, we established a co-culture system of human NP cells and vascular endothelial (HMEC-1) cells. We found that normal NP cells were mo...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Sun Z, Wan ZY, Guo YS, Wang HQ, Luo ZJ Tags: Int J Clin Exp Pathol Source Type: research

Hemocoagulase atrox reduces vascular modeling in rabbit carotid artery adventitia.
CONCLUSION: Our results suggested that hemocoagulase atrox as a topical hemostatic is safety and efficiently and it can accelerate adventitia restoration and decrease intimal proliferation. PMID: 24228100 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Wan SY, Hu YC, Zhan YQ, Qin DD, Ding Y Tags: Int J Clin Exp Pathol Source Type: research

Expression analysis of URI/RMP gene in endometrioid adenocarcinoma by tissue microarray immunohistochemistry.
Abstract Multiple studies have recently demonstrated the oncogenic property of URI (or RMP, a member of the prefoldin family of molecular chaperones) during progression of hepatocellular carcinoma, ovarian cancer, and possibly prostate cancer. Most recently, we have shown that URI/RMP is up-regulated in cervical cancer, another reproductive system tumor beside ovarian and prostate cancers. To investigate if URI/RMP also plays a role in other reproductive system tumors, especially in endometrioid adenocarcinoma, we analyzed URI/RMP expression in a TMA (tissue microarray) containing tissues from 30 cases of endometr...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Gu J, Liang Y, Qiao L, Li X, Li X, Lu Y, Zheng Q Tags: Int J Clin Exp Pathol Source Type: research

DGKα DNA vaccine relieves airway allergic inflammation in asthma model possibly via induction of T cell anergy.
DGKα DNA vaccine relieves airway allergic inflammation in asthma model possibly via induction of T cell anergy. Int J Clin Exp Pathol. 2013;6(11):2404-11 Authors: Wang Y, Zhang Q, Ma Q, Zhang Y, Li Z, Wang C Abstract Induction of T cell immune tolerance is thought to be a good method for treatment of asthma. Diacylglycerol kinases alpha (DGKα), enzymes that catalyze phosphorylation of diacylglycerol to produce phosphatidic acid, could inhibit diacylglycerol (DAG)-mediated signaling following T-cell receptor engagement and prevent T cell hyperactivation, thus playing important roles in the induc...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Wang Y, Zhang Q, Ma Q, Zhang Y, Li Z, Wang C Tags: Int J Clin Exp Pathol Source Type: research

In vivo biocompatibility of the PLGA microparticles in parotid gland.
L, Vilos C Abstract Poly(lactic-co-glycolic acid) (PLGA) microparticles are used in various disorders for the controlled or sustained release of drugs, with the management of salivary gland pathologies possible using this technology. There is no record of the response to such microparticles in the glandular parenchyma. The purpose of this study was to assess the morphological changes in the parotid gland when injected with a single dose of PLGA microparticles. We used 12 adult female Sprague Dawley rats (Rattus norvegicus) that were injected into their right parotid gland with sterile vehicle solution (G1, n=4), ...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Cantín M, Miranda P, Suazo Galdames I, Zavando D, Arenas P, Velásquez L, Vilos C Tags: Int J Clin Exp Pathol Source Type: research

Expression of aldo-keto reductase family 1 member C3 (AKR1C3) in neuroendocrine tumors & adenocarcinomas of pancreas, gastrointestinal tract, and lung.
In this report, we studied the expression of AKR1C3 in normal tissue, adenocarcinomas (43 cases) and neuroendocrine (NE) tumors (40 cases) arising from the aerodigestive tract and pancreas. We demonstrated wide expression of AKR1C3 in superficially located mucosal cells, but not in NE cells. AKR1C3-positive immunoreactivity was detected in 38 cases (88.4%) of adenocarcinoma, but only in 7 cases (17.5%) of NE tumors in all cases. All NE tumors arising from the pancreas and appendix and most tumors from the colon and lung were negative. The highest ratio of positive AKR1C3 in NE tumors was found in tumors arising from the sm...
Source: International Journal of Clinical and Experimental Pathology - November 16, 2013 Category: Pathology Authors: Chang TS, Lin HK, Rogers KA, Brame LS, Yeh MM, Yang Q, Fung KM Tags: Int J Clin Exp Pathol Source Type: research