"Int J Clin Exp Pathol"[ta]; +121 new citations
121 new pubmed citations were retrieved for your search. Click on the search hyperlink below to display the complete search results: "Int J Clin Exp Pathol"[ta] These pubmed results were generated on 2014/10/26PubMed, a service of the National Library of Medicine, includes over 15 million citations for biomedical articles back to the 1950's. These citations are from MEDLINE and additional life science journals. PubMed includes links to many sites providing full text articles and other related resources. (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - October 26, 2014 Category: Pathology Tags: Report Source Type: research

A guide to histomorphological evaluation of intestinal inflammation in mouse models.
AA Abstract Histomorphology remains a powerful routine evaluating intestinal inflammation in animal models. Emphasizing the focus of a given animal study, histopathology can overstate differences between established models. We aimed to systematize histopathological evaluation of intestinal inflammation in mouse models facilitating inter-study comparisons. Samples of all parts of the intestinal tract from well-established mouse models of intestinal inflammation were evaluated from hematoxylin/eosin-stained sections and specific observations confirmed by subsequent immunohistochemistry. Three main categories suffic...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Erben U, Loddenkemper C, Doerfel K, Spieckermann S, Haller D, Heimesaat MM, Zeitz M, Siegmund B, Kühl AA Tags: Int J Clin Exp Pathol Source Type: research

Venlafaxine inhibits apoptosis of hippocampal neurons by up-regulating brain-derived neurotrophic factor in a rat depression model.
CONCLUSION: Venlafaxine improved the expression of BDNF through working on PI3k/PKB/eNOS pathway and repressed the apoptosis of hippocampal neurons. PMID: 25197330 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Huang X, Mao YS, Li C, Wang H, Ji JL Tags: Int J Clin Exp Pathol Source Type: research

Protective effects of 2-deoxy-D-glucose on nephrotoxicity induced by cyclosporine A in rats.
CONCLUSIONS: 2-DG had obvious protective effect on nephrotoxicity especially with high dose. This protective effect could be related to the reduction of ROS induced by CsA. However, 2-DG had no effect on the expression of RIP3. PMID: 25197331 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Ouyang Z, Cao W, Zhu S, Liu X, Zhong Z, Lai X, Deng H, Jiang S, Wang Y Tags: Int J Clin Exp Pathol Source Type: research

Let-7g reverses malignant phenotype of osteosarcoma cells by targeting Aurora-B.
Abstract Accumulating studies revealed that the expression levels of several miRNAs are up or down-regulated in osteosarcoma (OS). The aim of this study was to investigate the functional significance and molecular of the let-7g in OS cells. The expression levels of let-7g was significantly down-regulated in OS cell lines U2-OS and HOS cell compared to osteoblast cell lines HOB cell. Moreover, bioinformatic prediction suggested that Aurora-B, which is overexpressed and functions as an oncogene in OS cells, is a putative target gene of let-7g. Using mRNA and protein expression analysis and luciferase assays, we furt...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Liu JM, Long XH, Zhang GM, Zhou Y, Chen XY, Huang SH, Liu ZL, Zhang ZH Tags: Int J Clin Exp Pathol Source Type: research

Epigallocatechin-3-gallate protects against cisplatin nephrotoxicity by inhibiting the apoptosis in mouse.
Abstract Cisplatin (CP) is a commonly used anticancer drug, but its notable side effect of nephrotoxicity limits its use in clinic. Epigallocatechin-3-gallate (EGCG), an anti-oxidant, anti-inflammatory, and anti-tumorigenic green tea polyphenol, has been available on the market for its beneficial effects. The aim of this study was to investigate whether EGCG can prevent the nephrotoxic effect of CP and the involved mechanisms. Male C57/BL6 mice were randomly divided into four groups: control group, EGCG group, CP group, and CP+EGCG group. On day 5, mice were sacrificed. Our results showed that EGCG treatment signi...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Zou P, Song J, Jiang B, Pei F, Chen B, Yang X, Liu G, Hu Z Tags: Int J Clin Exp Pathol Source Type: research

Effects of methylprednisolone, dantrolene, and their combination on experimental spinal cord injury.
This study aimed to evaluate the effect of methylprednisolone sodium succinate, dantrolene sodium, and their combination on experimental spinal cord injury. We used 25 rats (Rattus norvegicus) that were divided into five groups. The negative control group (NC) consisted of animals without spinal cord trauma. In the groups with spinal cord trauma, the positive control group (PC) was given no treatment, the MS group was treated with methylprednisolone, the MS/DS group was treated with methylprednisolone and dantrolene, and the DS group was treated with dantrolene alone. The animals' motor function was evaluated daily, as mea...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Rosado IR, Lavor MS, Alves EG, Fukushima FB, Oliveira KM, Silva CM, Caldeira FM, Costa PM, Melo EG Tags: Int J Clin Exp Pathol Source Type: research

Effects of early and late adverse experiences on morphological characteristics of Sprague-Dawley rat liver subjected to stress during adulthood.
Sol M Abstract The literature indicates that early rupture of the maternal bond and social isolation are variables involved in social and emotional behaviors and in increase in anxiety, particularly in stressful situations. The liver plays a role in the adaptation to stress, yet the possible morphologic changes that its structure can suffer have been studied very little. Therefore, the aim here was to ascertain, through the model of altering the early mother-infant bond and the late social bond through isolation, the effect on the stereologic characteristics of the liver in adult Sprague-Dawley rats subjected to i...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Vásquez B, Sandoval C, Smith RL, Del Sol M Tags: Int J Clin Exp Pathol Source Type: research

Design of a ProDer f 1 vaccine delivered by the MHC class II pathway of antigen presentation and analysis of the effectiveness for specific immunotherapy.
Abstract Dermatophagoides farinae (Der f 1) is one of leading cause for allergic asthma, and allergen-specific immunotherapy (SIT) is currently recognized as the only etiological therapy to ameliorate asthmatic symptom. The current study was designed on the major histocompatibility complex (MHC) class II pathway, invariant chain (Ii)-segment hybrids as vaccine basis to explore the efficacy of Der f 1 hybrid vaccine by virtue of Ii as carrier in enhancing the protective immune response to asthma. Initially, we engineered a fused molecule, DCP-IhC-ProDer f 1, to deliver ProDer f 1 antigen via specific dendritic cell...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Liu Z, Jiang Y, Li C Tags: Int J Clin Exp Pathol Source Type: research

Proteomic analysis of immature rat pups brain in response to hypoxia and ischemia challenge.
In this study, quantitative analysis was performed 24 hours after hypoxia-ischemia using liquid-chromatography mass spectrometry coupled to label-free analysis. Compared to control, 193 proteins were present only in hypoxic-ischemic group. In addition, 34 proteins were more than 2 folds up-regulated and 14 proteins were more than 2 folds down-regulated in hypoxia-ischemia group. Gene Ontology database showed that the majority of differentially expressed proteins comprised mitochondrial proteins et al. Molecular function analysis revealed that the majority of proteins were involved in ion binding et al. Biological process a...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Yang LJ, Ma DQ, Cui H Tags: Int J Clin Exp Pathol Source Type: research

Human amniotic fluid stem cells support undifferentiated propagation and pluripotency of human embryonic stem cell without b-FGF in a density dependent manner.
Abstract Human embryonic stem cells (hESCs) are pluripotent cells which can give rise to almost all adult cell lineages. Culture system of hESCs is complex, requiring exogenous b-FGF and feeder cell layer. Human mesenchymal stem cells (MSCs) not only synthesize soluble cytokines or factors such as b-FGF, but also provide other mechanism which might play positive role on sustaining hESCs propagation and pluripotency. Human amniotic fluid stem (AFS) cells, which share characteristics of both embryonic and adult stem cells, have been regarded as promising cells for regenerative medicine. Taking advantage by AFS cells...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Ma X, Li H, Xin S, Ma Y, Ouyang T Tags: Int J Clin Exp Pathol Source Type: research

Sildenefil increases connexin 40 in smooth muscle cells through activation of BMP pathways in pulmonary arterial hypertension.
CONCLUSIONS: Taken together, these data suggest the sildenafil activate BMP-Cx40 signaling in the PAH. This axis may be a potential therapeutic target in PAH. PMID: 25197339 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Yang L, Yin N, Hu L, Fan H, Yu D, Zhang W, Wang S, Feng Y, Fan C, Cao F, Mo X Tags: Int J Clin Exp Pathol Source Type: research

Decreased intratumoral Foxp3 Tregs and increased dendritic cell density by neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer.
Abstract Although neoadjuvant chemotherapy (NACT) has been increasingly used to improve the outcome of advanced gastric cancer (GC) for decades, its precise efficacy has been difficult to evaluate yet. Abundant studies have investigated the predictive factors that represent the effect of NACT on advanced GC. In the present study, the intratumoral infiltration of regulatory T cells (Tregs) and dendritic cells (DCs) response to NACT in advanced GC and their correlation with prognosis were evaluated. Infiltration of Tregs (marked by Foxp3) and DCs (marked by S-100) in 102 advanced GC specimens with or without NACT wa...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Hu M, Li K, Maskey N, Xu Z, Peng C, Wang B, Li Y, Yang G Tags: Int J Clin Exp Pathol Source Type: research

Decreased SFRP2 expression is associated with intermediate and poor karyotypes in de novo acute myeloid leukemia.
This study was aimed to analyze the expression status of SFRP2 gene in AML patients and explore its clinical significance using real-time quantitative PCR (RQ-PCR). The level of SFRP2 expression significantly decreased in AML compared to controls (P
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Guo H, Lin J, Wen XM, Yang J, Qian W, Deng ZQ, Ma JC, Tang CY, An C, Liu Q, Zhou H, Qian J Tags: Int J Clin Exp Pathol Source Type: research

STR DNA genotyping of hydatidiform moles in South China.
CONCLUSION: STR DNA genotyping is effective for diagnostic measure to precisely classify hydatidiform moles. And in the absence of laser capture microdissection (LCM), hematoxylin staining plus manual dissection under microscopic guided is a more economic and practical method. PMID: 25197342 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Zheng XZ, Hui P, Chang B, Gao ZB, Li Y, Wu BQ, Zhang B Tags: Int J Clin Exp Pathol Source Type: research

Role of biphasic changes in splenic dendritic cell activity in a mouse model of multiple organ dysfunction syndrome.
Abstract To analyze the changes in splenic dendritic cell (DC) activity and serum cytokine levels during the progression of multiple organ dysfunction syndrome (MODS). A C57BL/6 mouse model of MODS was established by intraperitoneal injection of zymosan. Immunohistochemistry and flow cytometry were used to detect expression of I-A(b) (MHC-II molecules of mice) as well as co-stimulatory and co-inhibitory molecules in spleen and DC surface. The levels of various cytokines in serum and spleen tissue were analyzed 6 h, 12 h, 24 h, 48 h, 5 d and 12 d after injury. Death occurred at 24-48 h and 10-12 d after injury. The...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Lv Y, Liu Q, Zhao M, Jin Y, Lu J Tags: Int J Clin Exp Pathol Source Type: research

Hyperbaric oxygen preconditioning promotes neovascularization of transplanted skin flaps in rats.
Abstract To determine whether Hyperbaric oxygen preconditioning (HBO-PC) promotes neovascularization by increasing Stromal cell derived factor-1 (SDF-1) and CXC chemokine receptor 4 (CXCR4) in transplanted skin flaps of rats. The epigastric pedicle skin flap was established in a rat model. Rats were randomly assigned to the following five groups: 1) sham-operated group (SH); 2) ischemia followed by reperfusion 3 days postoperatively group (IR3d); 3) ischemia followed by reperfusion 5 days postoperatively group (IR5d); 4) hyperbaric oxygen preconditioning and ischemia followed by reperfusion 3 days postoperatively ...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Liu X, Yang J, Li Z, Yang L, Wang C, Gao C, Liang F Tags: Int J Clin Exp Pathol Source Type: research

Different carcinogenic process in cholangiocarcinoma cases epidemically developing among workers of a printing company in Japan.
This study examined features of the carcinogenic process of the cholangiocarcinoma developed in the printing company. Surgically resected specimens of the cholangiocarcinoma cases were analyzed, where all cases were associated with precursor lesions such as biliary intraepithelial neoplasia (BilIN) and/or intraductal papillary neoplasm of the bile duct (IPNB). Immunohistochemical analysis confirmed constitutional expression of GST T1-1 in normal hepatobiliary tract. Immunostaining of γ-H2AX, a marker of DNA double strand break, showed that its expression was significantly increased in foci of BilIN, IPNB and invasive...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Sato Y, Kubo S, Takemura S, Sugawara Y, Tanaka S, Fujikawa M, Arimoto A, Harada K, Sasaki M, Nakanuma Y Tags: Int J Clin Exp Pathol Source Type: research

Magnesium isoglycyrrhizinate protects hepatic L02 cells from ischemia/reperfusion induced injury.
This study aimed to investigate the effects of Magnesium Isoglycyrrhizinate (MgIG) on the changes of oxidant stress and apoptosis induced by IRI in human hepatic L02 cells. L02 cells with IRI were treated with or without MgIG and mitoKATP (Mitochondrial adenosine triphosphate-dependent potassium) channel modulators. Cell viability was assessed using CCK-8 assay. Cell apoptosis was quantified by flow cytometry. The activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. Effects of MgIG on the expression of Bax, Bcl-2, Caspase 3, PARP (poly ADP-ribose polymerase), Akt...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Huang X, Qin J, Lu S Tags: Int J Clin Exp Pathol Source Type: research

Thioredoxin-1 inhibitor PX-12 induces human acute myeloid leukemia cell apoptosis and enhances the sensitivity of cells to arsenic trioxide.
Abstract Thioredoxin-1 (Trx-1), an important redox regulatory factor, plays a significant role in drug-induced apoptosis. Here we investigated the effects of the Trx-1 inhibitor 1-methylpropyl 2-imidazolyl disulfide (PX-12) on human acute myeloid leukemia cells (AML) and the sensitivity of cells to arsenic trioxide (As2O3, ATO). Treatment of cells with a different concentration of PX-12 for 48 h resulted in growth inhibition, the induction of apoptosis and increased the levels of activated caspase-3 expression in AML cell lines HL-60, NB4, U937 and primary AML cells in a dose-dependent manner. In addition, PX-12 e...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Tan Y, Bi L, Zhang P, Wang F, Lin F, Ni W, Wu J, Jiang L Tags: Int J Clin Exp Pathol Source Type: research

Decreased expression of SEMA3A is associated with poor prognosis in gastric carcinoma.
This study investigated the expression of SEMA3A in gastric cancer and its prognostic value for gastric cancer patients. METHODS: We examined the expression of SEMA3A in paired cancerous and matched adjacent noncancerous gastric mucosa tissues by real-time quantitative RT-PCR (qRT-PCR) and western blotting. In vitro, we evaluate the effects of SEMA3A on gastric cancer cell proliferation and migration by MTT, transwell and wound-healing assays. Furthermore, we analyzed SEMA3A expression in 128 patients who underwent resection procedures using immunohistochemistry. The relationships between the SEMA3A expression levels,...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Tang C, Gao X, Liu H, Jiang T, Zhai X Tags: Int J Clin Exp Pathol Source Type: research

Sox9 regulates hyperexpression of Wnt1 and Fzd1 in human osteosarcoma tissues and cells.
This study investigates the expression of Sox9, Wnt1 and Fzd1 in human osteosarcoma tissues and cells and the role of Sox9 in the proliferation of human osteosarcoma cells. Immunohistochemical analyses for Sox9, Wnt1, Fzd1, and Ki-67 proteins were performed in human primary osteosarcoma tissues from 48 patients. The small interfering RNA (siRNA) of Sox9 was transfected into human osteosarcoma MG63 cells. At 24 and 48 h after transfection with Sox9 siRNA, the expression of Wnt1 and Fzd1 was analyzed by RT-qPCR, Western blot, and immunofluorescence techniques. Cell proliferation was assayed by CCK-8 method, and Ki-67 protein...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Liu H, Chen Y, Zhou F, Jie L, Pu L, Ju J, Li F, Dai Z, Wang X, Zhou S Tags: Int J Clin Exp Pathol Source Type: research

Sarcomatoid variant of ALK(-) anaplastic large cell lymphoma involving multiple lymph nodes and both lungs with production of proinflammatory cytokines: report of a case and review of literature.
We report here such a case of ALCL with both pulmonary and multiple nodal involvement in a 47-year-old woman who initially presented with fever, cough, sputum, itching skin, and weight loss. The initial transbronchial lung biopsy showed discohesive pleomorphic malignant cells in a strong inflammatory milieu reminiscent of inflammatory malignant fibrous histiocytoma (MFH). Subsequent cervical lymph node biopsy revealed a spindle cell sarcoma predominantly composed of plump spindle and oval neoplastic cells in interweaving fascicles, with sparse inflammatory infiltrates, resembling pleomorphic-storiform type of MFH. However,...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Yu L, Yan LL, Yang SJ Tags: Int J Clin Exp Pathol Source Type: research

CBX8, a novel DNA repair protein, promotes tumorigenesis in human esophageal carcinoma.
In this study, we found that CBX8 was up-regulated in esophageal carcinoma tissues compared with adjacent non-cancerous tissues (P
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Xiao W, Ou C, Qin J, Xing F, Sun Y, Li Z, Qiu J Tags: Int J Clin Exp Pathol Source Type: research

Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells.
This study was designed to investigate the effects of TGF-β2 on MYOC expression and secretion in human primary cultured TM cells. Primary cultured human TM cells were treated with 0 (control group), 1, 10, and 100 ng/mL TGF-β2 for 12, 24, or 48 h. MYOC mRNA and protein expressions in TM cells and protein secretion in conditioned media were analyzed by semi-quantitative RT-PCR, Western blotting, and enzyme-linked immunosorbent assays (ELISA), respectively. TM cells treated with 1, 10, and, 100 ng/mL TGF-β2 for 48 h showed higher MYOC mRNA and protein expressions than those in the control group (0 ng/mL TGF-β2) (all P
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Wu Y, Chen W, Guo M, He Q, Hu Y Tags: Int J Clin Exp Pathol Source Type: research

Clinicopathological features of pulmonary cryptococcosis with cryptococcal titan cells: a comparative analysis of 27 cases.
Abstract In addition to the typical size, Cryptococcus neoformans can enlarge its size to form titan cells during infection, and its diameter can reach up to 100 μm. Clinical reports about cryptococcal titan cells are rare. Most studies focus on aspects of animal models of infection with titan cells. Herein, we report the clinical and imaging characteristics and histopathologic features of 3 patients with titan cells and 27 patients with pathogens of typical size, and describe the morphological characteristics of titan cells in details. Histologically, 3 patients with titan cells show necrosis, fibrosis and mac...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Wang JM, Zhou Q, Cai HR, Zhuang Y, Zhang YF, Xin XY, Meng FQ, Wang YP Tags: Int J Clin Exp Pathol Source Type: research

Concordant podoplanin expression in cancer-associated fibroblasts and tumor cells is an adverse prognostic factor in esophageal squamous cell carcinoma.
Abstract There is growing evidence that cancer-associated fibroblasts (CAFs) interact with tumor cells and play important roles in tumor progression and invasion. Podoplanin is a type-1 transmembrane glycoprotein expressed in a variety of normal human tissues, including lymphatic endothelium. Tumor cell expression of podoplanin correlates with nodal metastasis and poor prognosis in squamous cell carcinoma (SCC) of oral cavity and esophagus. Recently, podoplanin-positive CAFs have been shown to exert adverse or beneficial prognostic effect on different cancer types. However, the significance of podoplanin-positive ...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Chuang WY, Yeh CJ, Chao YK, Liu YH, Chang YS, Tseng CK, Chang HK, Wan YL, Hsueh C Tags: Int J Clin Exp Pathol Source Type: research

Glioblastoma microvesicles promote endothelial cell proliferation through Akt/beta-catenin pathway.
In conclusion, glioblastoma mainly affects microvesicles within CSF without showing significant impact on microvesicles in circulating blood. Microvesicles from the CSF of glioblastoma patients may initiate endothelial cell growth and thus promote cell invasion. This effect may be directly exerted by activated Akt/beta-catenin pathway. PMID: 25197356 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Liu S, Sun J, Lan Q Tags: Int J Clin Exp Pathol Source Type: research

Identification of miR-140-3p as a marker associated with poor prognosis in spinal chordoma.
CONCLUSION: Over-expression of miR-140-3p is correlated with recurrence and tumor invasion, suggesting that miR-140-3p could be a new predictor for recurrence and prognosis in patients with spinal chordoma. PMID: 25197358 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Zou MX, Huang W, Wang XB, Lv GH, Li J, Deng YW Tags: Int J Clin Exp Pathol Source Type: research

Anti-inflammatory effect of peroxisome proliferator-activated receptor-γ (PPAR-γ) on non-obese diabetic mice with Sjogren's syndrome.
Anti-inflammatory effect of peroxisome proliferator-activated receptor-γ (PPAR-γ) on non-obese diabetic mice with Sjogren's syndrome. Int J Clin Exp Pathol. 2014;7(8):4886-94 Authors: Li X, Xu B, Wang Y, Wei L Abstract OBJECTIVE: To investigate the anti-inflammatory effect of peroxisome proliferator-activated receptor-γ (PPAR-γ) on non-obese diabetic mice (NOD mice) with Sjogren's syndrome. METHODS: 22 eight-week-old female NOD mice were randomly divided into 2 groups. Rosiglitazone and normal saline were administered in the PPAR-γ group and the control group respect...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Li X, Xu B, Wang Y, Wei L Tags: Int J Clin Exp Pathol Source Type: research

Primary microcephaly gene MCPH1 shows a novel molecular biomarker of human renal carcinoma and is regulated by miR-27a.
Abstract Microcephalin 1 (MCPH1) gene, initially identified as an hTERT repressor, result in two autosomal recessive disorders: primary microcephaly and premature chromosome condensation syndrome. Recently, several studies have found that MCPH1 has also been shown to be downregulated in several different types of human cancers, suggesting that it could also function as a tumor suppressor gene and a novel molecular biomarker of human cancers. To investigate its potential role in the human renal carcinoma progression, we evaluated the expression of protein MCPH1 in 188 renal cancer and 20 normal renal tissues from 1...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Wang N, Lu H, Chen W, Gan M, Cao X, Zhang J, Chen L Tags: Int J Clin Exp Pathol Source Type: research

Spindle cell rhabdomyosarcoma of the retroperitoneum: an unusual case developed in a pregnant woman but obscured by pregnancy.
We describe a rare case of spindle cell RMS of the retroperitoneum in a 37-year-old woman developed during pregnancy and incidentally discovered after vaginal delivery. Computed tomography showed a huge tumor mass, measured 20 × 20 × 15 cm in size, arising in retroperitoneal space. Histologically, the tumor consisted of spindle cells arranged in a fascicular or herringbone growth pattern, morphologically mimicking adult fibrosarcoma, intermingled with scattered rhabdomyoblasts. Mitotic activity ranged from 20 to 28 mitoses per 10 high-power fields and tumor necrosis was evident. Immunohistochemically, tumor cel...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Yu L, Yang SJ Tags: Int J Clin Exp Pathol Source Type: research

Autophagy may promote carcinoma cell invasion and correlate with poor prognosis in cholangiocarcinoma.
This study investigated its involvement in cholangiocarcinoma, focusing on carcinoma cell invasion and prognostic significance using cholangiocarcinoma cell lines, CCKS1 and HuCCT1, and human tissues of hilar and extrahepatic cholangiocarcinoma. Nutrient starvation induced the expression of LC3-II and the formation of LC3 puncta in both CCKS1 and HuCCT1, suggesting the occurrence of autophagy. The induction of autophagy was accompanied by the increased expression of an autophagy-related protein, Ambra1, in the cells. Under starvation conditions, the invasive activity of both cells was significantly increased, and a lysosom...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Nitta T, Sato Y, Ren XS, Harada K, Sasaki M, Hirano S, Nakanuma Y Tags: Int J Clin Exp Pathol Source Type: research

Systemically transplanted human gingiva-derived mesenchymal stem cells contributing to bone tissue regeneration.
In this study, we transplanted human GMSCs into C57BL/6J mice with defects in mandibular bone via the tail vein to explore the capacity of transplanted GMSCs to promote bone regeneration. Results showed that the transplanted GMSCs were detected in the bone defects and employed in new bone formation. And the newly formed bone area in mice with GMSCs transplantation was significantly higher than that in control mice. Our findings indicate that systemically transplanted GMSCs can not only home to the mandibular defect but also promote bone regeneration. PMID: 25197363 [PubMed - in process] (Source: International Journal ...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Xu QC, Wang ZG, Ji QX, Yu XB, Xu XY, Yuan CQ, Deng J, Yang PS Tags: Int J Clin Exp Pathol Source Type: research

Deubiquitination and stabilization of IL-33 by USP21.
Abstract Interleukin-33 (IL-33) is a dual-function protein that acts both as a secreted cytokine and as a nuclear factor regulating gene transcription. It has been demonstrated that IL-33 exerts its nuclear function in promoting the transcription of NF-κB p65. Here, we show that USP21-mediated deubiquitination of IL-33 affects the transcription of p65. IL-33 can be post-translationally modified by ubiquitination. Ubiquitin-specific protease 21 (USP21) interacts with IL-33 and also localizes in nucleus. The protein stability of IL-33 is maintained by USP21 through deubiquitination. Furthermore, depletion of U...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Tao L, Chen C, Song H, Piccioni M, Shi G, Li B Tags: Int J Clin Exp Pathol Source Type: research

In vitro expression and development of indirect ELISA for Capsid protein of duck circovirus without nuclear localization signal.
Abstract Duck circovirus was a newly discovered pathogen that causing ducks immunosuppression in recent years, but it can not been cultured in vitro that limited its depth study. In the present study, the Cap gene that defect the nuclear localization signal (NLS) of DuCV was amplified and connected to the express vector pET-32a (+), to express the recombinant Cap protein in the bacterium Escherichia coli Rosetta. The recombinant Cap protein was purified and an indirect ELISA method was developed based on the recombinant Cap protein. The results showed that the truncated Cap gene was 567 bp and cloned into pET-32a ...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Lu Y, Jia R, Zhang Z, Wang M, Xu Y, Zhu D, Chen S, Liu M, Yin Z, Chen X, Cheng A Tags: Int J Clin Exp Pathol Source Type: research

Prognostic significance of mucin expression in urothelial bladder cancer.
Abstract Urothelial bladder cancer (UBC) is a common genitourinary malignancy, accounting for more than 160.000 deaths per year worldwide. Overexpression and aberrant glycosylation of mucins are frequent traits of many human cancers derived from epithelial cells, and are found to have prognostic significance in various carcinomas. The aim of this study was to further elucidate the features and significance of mucin expression in UBC. We investigated the relationship between mucin expression and clinicopathological characteristics in 539 cases of UBC by immunohistochemical analysis of MUC1, MUC2, MUC4, MUC5AC and M...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Stojnev S, Ristic-Petrovic A, Velickovic LJ, Krstic M, Bogdanovic D, Khanh do T, Ristic A, Conic I, Stefanovic V Tags: Int J Clin Exp Pathol Source Type: research

Somatostatin receptor immunohistochemistry in neuroendocrine tumors: comparison between manual and automated evaluation.
CONCLUSION: Our data demonstrate that the evaluation of the SSTR status by automated analysis (BB1 score), using digitized histopathology slides ("virtual microscopy"), corresponds well with the SSTR2A, 4 and 5 expression as determined by conventional manual histopathology. The BB1 score also exhibited a significant association to the SSTR-PET/CT data in accordance with the high affinity profile of the SSTR analogues used for imaging. PMID: 25197368 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Daniel K, Maria A, Amelie L, Isabell L, Stefan S, Luisa P, Merten H, Vikas P, Gerd B, Paul BR Tags: Int J Clin Exp Pathol Source Type: research

Subtotal splenectomy for splenomegaly in cirrhotic patients.
CONCLUSION: After subtotal splenectomy, the residual spleen will not experience a high-pressure environment, and the fibrosis of splenic tissue and remodelling of corpuscular morphology will cease. PMID: 25197369 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Chu H, Liu X, Zhao J, Xu Y, Wang L, Wang T, Guo W, Zhang S, Zhu X Tags: Int J Clin Exp Pathol Source Type: research

Clinicopathological characteristics of four cases of EBV positive T-cell lymphoproliferative disorders of childhood in China.
In this study, we describe the distinct features of four cases of EBV positive T-cell LPD of childhood in China. Two were systemic EBV positive T-cell LPD of childhood, one was HVLL and one was chronic active EBV (CAEBV). The main manifestations were lymphadenopathy, fever, hepatosplenomegaly and skin rashes. The structure of the lymph nodes in the patients ranged from preserved to partially or totally destroyed. Small- to medium-sized, atypical T cells had infiltrated the lymph nodes. In HVLL, the neoplastic cells had infiltrated the dermis and subcutaneous region surrounding sweat glands and nerves. All of the cases test...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Huang W, Lv N, Ying J, Qiu T, Feng X Tags: Int J Clin Exp Pathol Source Type: research

Upregulation of miR-137 protects anesthesia-induced hippocampal neurodegeneration.
CONCLUSIONS: Our results demonstrated that miR-137 played an important role in regulating ketamine induced hippocampal neurodegeneration, possibly through CDC42. PMID: 25197371 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Huang C, Zhang X, Zheng J, Chen C, Chen Y, Yi J Tags: Int J Clin Exp Pathol Source Type: research

Lycopene ameliorates renal function in rats with streptozotocin-induced diabetes.
CONCLUSIONS: Lycopene protects against development of diabetic nephropathy and ameliorates renal function via improving oxidative status and regulating p-Akt and CTGF. PMID: 25197372 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Li W, Wang G, Lu X, Jiang Y, Xu L, Zhao X Tags: Int J Clin Exp Pathol Source Type: research

Comparison of bone marrow mesenchymal stem cells and core decompression in treatment of osteonecrosis of the femoral head: a meta-analysis.
Abstract The study aims to compare the clinical efficacy of core decompression (CD) and bone marrow mesenchymal stem cells (BMMSC) on the patients with osteonecrosis of the femoral head (ONFH). A detailed literature search of PubMed, MEDLINE and EMBASE, Springer, Elsevier Science Direct, Cochrane Library and Google scholar for all relevant papers published was performed. Pooled odds ratio (OR) or weighted mean differences (WMD) and 95% confidence interval (CI) were used to evaluate the clinical efficacy of CD and BMMSC with the clinical outcome on the patients with ONFH. A total of 219 hips in 4 studies were inden...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Li X, Xu X, Wu W Tags: Int J Clin Exp Pathol Source Type: research

Prognostic significance of astrocyte elevated gene-1 in human astrocytomas.
This study aimed to investigate the expression of AEG-1 in human astrocytomas and the correlation between AEG-1 expression and clinicopathologic variables of astrocytomas. AEG-1 expression in four pairs of matched astrocytomas tissues and 5 cell lines was detected by immunohistochemical and Western blot analysis. In addition, AEG-1 protein expression was examined by immunohistochemical staining in 204 cases, including 32 normal brain tissues, 80 Low-malignant astrocytomas (LMAs) and 92 High-Malignant astrocytomas (HMAs). AEG-1 expression in 31 LMAs and 29 HMAs samples was detected by RT-PCR and Western blot analysis. We de...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: He Z, He M, Wang C, Xu B, Tong L, He J, Sun B, Wei L, Chu M Tags: Int J Clin Exp Pathol Source Type: research

Cystic lymphangioma of adrenal gland: a clinicopathological study of 3 cases and review of literature.
Abstract Cystic lymphangioma of the adrenal gland is a rare and benign lesion, most often found incidentally during abdominal imaging studies, abdominal surgery or at autopsy. We aimed to retrospectively review all adrenal lymphangioma cases at our hospital, further document their lymphatic origin by immunohistochemical staining and discuss the differential diagnosis with other cystic adrenal gland lesions. A total of 3 adrenal lymphangioma cases were identified. All three patients were men and adults at time of diagnosis aged 41 years, 43 years, and 66 years, respectively. All were incidentally identified during ...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Zhao M, Gu Q, Li C, Yu J, Qi H Tags: Int J Clin Exp Pathol Source Type: research

Genome-wide screening of DNA copy number alterations in cervical carcinoma patients with CGH+SNP microarrays and HPV-FISH.
This study also indicates that CGH+SNP microarrays allow detecting genome-wide CNAs and copy-neutral loss of heterozygosity more precisely, however, it may be less sensitive than FISH in samples with low level clonal CNAs. PMID: 25197380 [PubMed - in process] (Source: International Journal of Clinical and Experimental Pathology)
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Kuglik P, Smetana J, Vallova V, Moukova L, Kasikova K, Cvanova M, Brozova L Tags: Int J Clin Exp Pathol Source Type: research

Lin28B is a novel prognostic marker in gastric adenocarcinoma.
We examined the expression of Lin28B in 97 human gastric cancer samples with 32 samples of non-dysplastic tissues by immunohistochemistry. In the 97 GAC cases, 42 were with high Lin28B expression. The expression levels of Lin28B proteins in GAC were higher than in corresponding adjacent normal tissues (P=0.001). Significant correlations were noted between Lin28B expression and lymph node status (P=0.005), TNM stage (P
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Hu Q, Peng J, Liu W, He X, Cui L, Chen X, Yang M, Liu H, Liu S, Wang H Tags: Int J Clin Exp Pathol Source Type: research

Polymorphism in miRNA-1 target site and circulating miRNA-1 phenotype are associated with the decreased risk and prognosis of coronary artery disease.
Abstract MiRNA molecules have been identified to play key roles in a broad range of physiologic and pathologic processes. Polymorphisms in microRNA target sites (PolymiRTSs) can disturb or obstruct miRNA binding and consequentially influence regulation of the target genes. A two-step study design was used in this study. A case-control study was designed to assess the relationship between miRNA-1 target site rs9548934C→T polymorphism in target gene (Component of Oligomeric Golgi Complex 6, COG6) and risk of coronary artery disease (CAD) in 1013 patients and 610 normal controls. This genetic variant was also ev...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Wang L, Zhi H, Li Y, Ma G, Ye X, Yu X, Yang T, Jin H, Lu Z, Wei P Tags: Int J Clin Exp Pathol Source Type: research

Association of pancreatic adenocarcinoma up-regulated factor expression in ovarian mucinous adenocarcinoma with poor prognosis.
In this study, we examined various clinicopathologic features of 24 patients with mucinous cystadenoma (MCA), 36 with mucinous borderline tumors (MBTs), and 46 with mucinous adenocarcinomas (MACs) according to PAUF expression status assessed using immunohistochemistry. We found that MACs more frequently stained positive for PAUF than did MCAs and MBTs (P
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Kim SK, Song SY, Kim S, Cho NH, Yim GW, Kim SW, Kim YT, Nam EJ Tags: Int J Clin Exp Pathol Source Type: research

Cytological features of lung adenocarcinoma with micropapillary pattern in the pleural or pericardial effusion: analysis of 5 cases.
This study was comprised of 5 consecutive cases of lung adenocarcinoma with micropapillary pattern, in which the tumor cells were present in the pleural or pericardial effusion and whose diagnoses were histopathologically confirmed. The characteristic cytological findings in the pleural or pericardial effusion were as follows: i) tightly cohesive small nests of tumor cells showing papillary structure without fibrovascular core, ii) these nests were comprised of approximately 5-20 tumor cells, iii) cauliflower-like and acinar-like structures were also observed, iv) intracytoplasmic vacuoles were observed in 40% of the cases...
Source: International Journal of Clinical and Experimental Pathology - September 15, 2014 Category: Pathology Authors: Ishida M, Kagotani A, Iwai M Tags: Int J Clin Exp Pathol Source Type: research