Implication of N-glycolylneuraminic acid in regulation of cell adhesiveness of C2C12 myoblast cells during differentiation into myotube cells
This study thus aims to gain a functional insight into this phenomenon. The following lines of evidence show that the increasedde novo synthesis of Neu5Gc residues in differentiating myoblast cells promotes adhesiveness of the cells, which is beneficial for promotion of differentiation. First, the Sia transition occurred even in the C2C12 cells cultured in serum-free medium, indicating that it happens throughde novo synthesis of Neu5Gc. Second, GM3(Neu5Gc) was localized in myoblast cells, but not in myotube cells, and related to expression of the CMP-Neu5Ac hydroxylase (CMAH) gene. Notably, expression ofCMAH precedes myotu...
Source: Glycoconjugate Journal - May 31, 2022 Category: Biochemistry Source Type: research
Advanced Glycation End Products: key player of the pathogenesis of atherosclerosis
AbstractAtherosclerosis is the most common type of cardiovascular disease, and it causes intima thickening, plaque development, and ultimate blockage of the artery lumen. Advanced glycation end products (AGEs) are thought to have a role in the development and progression of atherosclerosis. there is developing an enthusiasm for AGEs as a potential remedial target. AGES mainly induce arterial damage and exacerbate the development of atherosclerotic plaques by triggering cell receptor-dependent signalling. The interplay of AGEs with RAGE, a transmembrane signalling receptor present across all cells important to atheroscleros...
Source: Glycoconjugate Journal - May 17, 2022 Category: Biochemistry Source Type: research
GM2/GM3 controls the organizational status of CD82/Met microdomains: further studies in GM2/GM3 complexation
AbstractAt cell surface gangliosides might associate with signal transducers proteins, grown factor receptors, integrins, small G-proteins and tetraspanins establishing microdomains, which play important role in cell adhesion, cell activation, motility, and growth. Previously, we reported that GM2 and GM3 form a heterodimer that interacts with the tetraspanin CD82, controlling epithelial cell mobility by inhibiting integrin-hepatocyte growth factor-induced cMet tyrosine kinase signaling. By using molecular dynamics simulations to study the molecular basis of GM2/GM3 interaction we demonstrate, here, that intracellular leve...
Source: Glycoconjugate Journal - May 10, 2022 Category: Biochemistry Source Type: research
Preclinical development of the quadrivalent meningococcal (ACYW) tetanus toxoid conjugate vaccine, MenQuadfi ®
AbstractBacterial capsular polysaccharide vaccines are generally poorly immunogenic in infants and older adults. The immunogenicity of capsular polysaccharide vaccines can be improved by conjugating them to immunogenic carrier proteins. One of the most recently licensed conjugate vaccines is the quadrivalent meningococcal vaccine with serogroups A, C, Y, and W conjugated to a tetanus toxoid protein carrier (MenACYW-TT; MenQuadfi, Sanofi Pasteur, Swiftwater, PA, USA). MenACYW-TT was developed to induce optimal immune responses against each of the meningococcal serogroups A, C, W, and Y, and across all age groups, especially...
Source: Glycoconjugate Journal - April 20, 2022 Category: Biochemistry Source Type: research
“Glycans and Glycosylation in SARS-COV2 Infection” session at the XVII Advanced School in Carbohydrate Chemistry, Italian Chemical Society. July 4th -7th 2021, Pontignano (Si), Italy
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - April 20, 2022 Category: Biochemistry Source Type: research
Preclinical development of the quadrivalent meningococcal (ACYW) tetanus toxoid conjugate vaccine, MenQuadfi ®
AbstractBacterial capsular polysaccharide vaccines are generally poorly immunogenic in infants and older adults. The immunogenicity of capsular polysaccharide vaccines can be improved by conjugating them to immunogenic carrier proteins. One of the most recently licensed conjugate vaccines is the quadrivalent meningococcal vaccine with serogroups A, C, Y, and W conjugated to a tetanus toxoid protein carrier (MenACYW-TT; MenQuadfi, Sanofi Pasteur, Swiftwater, PA, USA). MenACYW-TT was developed to induce optimal immune responses against each of the meningococcal serogroups A, C, W, and Y, and across all age groups, especially...
Source: Glycoconjugate Journal - April 20, 2022 Category: Biochemistry Source Type: research
“Glycans and Glycosylation in SARS-COV2 Infection” session at the XVII Advanced School in Carbohydrate Chemistry, Italian Chemical Society. July 4th -7th 2021, Pontignano (Si), Italy
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - April 20, 2022 Category: Biochemistry Source Type: research
Structural characterization and biological activities of a new polysaccharide isolated from Morchella Sextelata
In this study, a new water-soluble polysaccharide (MSP-3-1) with an average molecular weight of 2.35 × 107 Da was extracted and purified from fruiting bodies of cultivatedM. Sextelata. The structural characterization and biological activities of purified polysaccharide was further investigated. The results indicated that MSP-3-1 was mainly a α-glucan, mainly consisting of mannose (Man), glucose (Glc) and galactose (Gal) in a ratio of 5.10: 91.39: 3.51. Its surface morphology exhibited irregular lamellar structures with small voids. And the particle size analysis showed that MSP-3-1 was the homogeneous nanoparticle in wat...
Source: Glycoconjugate Journal - April 13, 2022 Category: Biochemistry Source Type: research
Structural characterization of a novel polysaccharide from sweet corncob that inhibits glycosylase in STZ-induced diabetic rats
AbstractIn the current study, we extracted a polysaccharide from sweet corncob and evaluated its hypoglycemic function. After collection in water, alcohol precipitation, and purification by DEAE-52 and Sephadex G-100 columns, we obtained a polysaccharide (SCP50) that was composed primarily of mannose and glucose (9.73:190.27), with a molecular weight of 9280.33 Da. We demonstrated that SCP50 exhibited significant inhibition of α-glucosidase activity, with an IC50 of 4.866 mg/mL, Km of 1.297 × 10−3, and Vmax of 0.076 mol/L·min−1in vitro. We also observed that SCP50 markedly attenuated disaccharidase (maltas...
Source: Glycoconjugate Journal - April 7, 2022 Category: Biochemistry Source Type: research
Hyperglycemia alters N-glycans on colon cancer cells through increased production of activated monosaccharides
AbstractDiabetes Mellitus (DM) is both, correlated and a known risk factor for colorectal cancer (CRC). Besides favoring the incidence of CRC, DM also accelerates its progression, worsening its prognosis. Previously, hyperglycemia, the DM hallmark, has been shown to lead to aberrant glycosylation of CRC cells, heightening their malignancy bothin vivo andin vitro. Here we use mass spectrometry to elucidate the composition and putative structures of N-glycans expressed by MC38 cultured in normoglycemic (LG) and hyperglycemic-like conditions (HG). N-glycans, 67, were identified in MC38 cells cultured in LG and HG. The cells g...
Source: Glycoconjugate Journal - April 5, 2022 Category: Biochemistry Source Type: research
