The oligosaccharide portion of ganglioside GM1 regulates mitochondrial function in neuroblastoma cells
AbstractThe crucial role of ganglioside GM1 in the regulation of neural homeostasis has been assessed by several studies. Recently we shed new light on the molecular basis underlying GM1 effects demonstrating that GM1 oligosaccharide directly binds TrkA receptor and triggers MAPK pathway activation leading to neuronal differentiation and protection. Following its exogenous administration, proteomic analysis revealed an increased expression of proteins involved in several biochemical mechanisms, including mitochondrial bioenergetics. Based on these data, we investigated the possible effect of GM1 oligosaccharide administrat...
Source: Glycoconjugate Journal - April 8, 2020 Category: Biochemistry Source Type: research

Disruption of hepatocyte Sialylation drives a T cell-dependent pro-inflammatory immune tone
AbstractThrough the catalysis of α2,6-linked sialylation, the enzyme ST6Gal1 is thought to play key roles in immune cell communication and homeostasis. Of particular importance, glycans with terminal α2,6-sialic acids are known to negatively regulate B cell receptor signaling and are associated with an immunosuppressive tumor mic roenvironment that promotes T cell anergy, suggesting that α2,6-sialic acids are a key immune inhibitory signal. Consistent with this model, mice harboring a hepatocyte-specific ablation of ST6Gal1 (H-cKO) develop a progressive and severe non-alcoholic fatty liver disease charact...
Source: Glycoconjugate Journal - March 28, 2020 Category: Biochemistry Source Type: research

Gangliosides in the differentiation process of primary neurons: the specific role of GM1-oligosaccharide
In this study, we show that GM1-oligosaccharide administered to mice cerebellar granule neurons by interacting with cell surface induces TrkA-MAP kinase pathway activation enhancing neuron clustering, arborization and networking. Accordingly, in the presence of GM1-oligosaccharide, neurons show a higher phosphorylation rate of FAK and Src proteins, the intracellular key regulators of neuronal motility. Moreover, treated cells express increased level of specific neuronal markers, suggesting an advanced stage of maturation compared to controls. In parallel, we found that in the presence of GM1-oligosaccharide, neurons accele...
Source: Glycoconjugate Journal - March 20, 2020 Category: Biochemistry Source Type: research

Obituary for Roland Schauer
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - March 17, 2020 Category: Biochemistry Source Type: research

Upregulation of Fucosyltransferase 3, 8 and protein O-Fucosyltransferase 1, 2 genes in esophageal cancer stem-like cells (CSLCs)
AbstractRecently, studies have shown that Fucosylation plays an important role in the invasion and metastatic process of CSLCs. Understanding the expression pattern of fucosyltransferase (FUT) genes may help to suggest better-targeted therapy strategies for esophageal squamous cell carcinoma (ESCC). The study aimed to address the expression pattern of FUT gene variants in esophageal CSLCs and parental adherent cells. Sphere formation method was used to enrich CSLCs. Expression of FUT genes was examined in tumor sphere and parental adherent cells using the RT-PCR method and then relative expression of detected variants was ...
Source: Glycoconjugate Journal - March 10, 2020 Category: Biochemistry Source Type: research

Immunoregulation and antioxidant activities of a novel acidic polysaccharide from Radix Paeoniae Alba
AbstractRadix Paeoniae Alba is widely used in Chinese traditional medicine to treat various diseases such as gastrointestinal disorders, immunomodulatory, cancer, and other diseases. In this paper, a novel acidic polysaccharide RPAPS purified fromRadix Paeoniae Alba was evaluated for its structural features and potential of immunomodulatory and antioxidant activities. RPAPS (molecular weight: 1.0 × 105 Da) was mainly composed of α-(1 → 4)-Glcp, α-Arap, α-Galp, α-Rhap, β-D-Glcp, α-(1 → 6)-linked Glcp and GalA. Immunological tests indicated t...
Source: Glycoconjugate Journal - March 5, 2020 Category: Biochemistry Source Type: research

Abnormal Galactosylated –Glycans recognized by Bandeiraea Simplicifolia Lectin I in saliva of patients with breast Cancer
AbstractCurrently, the definitive diagnosis in breast cancer requires biopsy and histopathology, such the most effective markers are tissue-based. However, the advantages of saliva in collection and storage make it possible for assessing human pathology and contributing to the development of cancer-related biomarkers for clinical application. The present study validated alteration of salivary protein glycopatterns recognized by Bandeiraea simplicifolia lectin I (BS-I) in the saliva of patients with breast diseases using saliva microarrays, and the N/O-glycan profiles of their salivary glycoproteins isolated by the BS-I-mag...
Source: Glycoconjugate Journal - February 27, 2020 Category: Biochemistry Source Type: research

Glycosaminoglycan remodeling during chondrogenic differentiation of human bone marrow −/synovial-derived mesenchymal stem/stromal cells under normoxia and hypoxia
AbstractGlycosaminoglycans (GAGs) are major components of cartilage extracellular matrix (ECM), which play an important role in tissue homeostasis not only by providing mechanical load resistance, but also as signaling mediators of key cellular processes such as adhesion, migration, proliferation and differentiation. Specific GAG types as well as their disaccharide sulfation patterns can be predictive of the tissue maturation level but also of disease states such as osteoarthritis. In this work, we used a highly sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to perform a comparative study in ter...
Source: Glycoconjugate Journal - February 21, 2020 Category: Biochemistry Source Type: research

Logically derived sequence tandem mass spectrometry for structural determination of Galactose oligosaccharides
AbstractMass spectrometry has high sensitivity and is widely used in the identification of molecular structures, however, the structural determination of oligosaccharides through mass spectrometry is still challenging. A novel method, namely the logically derived sequence (LODES) tandem mass spectrometry (MSn), for the structural determination of underivatized oligosaccharides was developed. This method, which is based on the dissociation mechanisms, involves sequential low-energy collision-induced dissociation (CID) of sodium ion adducts, a logical sequence for identifying the structurally decisive product ions for subseq...
Source: Glycoconjugate Journal - February 15, 2020 Category: Biochemistry Source Type: research

The physiological and pathological roles and applications of sialyl Lewis x, a common carbohydrate ligand of the three selectins
AbstractIn the past decades, the roles of carbohydrates in living organisms and their potential use in many fields have been extensively investigated. Sialyl Lewis x (sLex), a member of body carbohydrate, is an inherent blood-type tetrasaccharide on the surface of different cells, the lymphocyte, neutrophil, some T cells, multiple tumor cells and so on. SLex is a common ligand of the three selectins, L-selectin, E-selectin and P-selectin, and plays important roles in multiple physiological phenomenas by interacting with selectins. Under normal physiological conditions, sLex can affect the immune process and fertilization p...
Source: Glycoconjugate Journal - February 15, 2020 Category: Biochemistry Source Type: research

High FUT3 expression is a marker of lower overall survival of breast cancer patients
AbstractThe complex enzyme network responsible for glycan synthesis suffers significant changes during the first steps of tumor development, leading to the early formation of tumor-associated glycan signatures. Among the glycosylation pathways, changes in fucosylation emerged as one of most important features in cancer. Αlpha-1,3/4-fucosyltransferase (FUT3) has been linked to pro-tumor and anti-tumor pathways depending on the cancer type. The present study aimed to understand the gene and protein expression profiles of FUT3 in three different and independent cohorts composed by invasive breast cancer patients: Loc al...
Source: Glycoconjugate Journal - February 15, 2020 Category: Biochemistry Source Type: research

Separation and preparation of N -glycans based on ammonia-catalyzed release method
In this study, we aimed to obtain large amounts of glycans using an optimized procedure. Two types of reductiveN-glycans were released from chicken egg albumin (ovalbumin) and soy protein using an ammonia catalysis method and labeled with benzenesulfonyl hydrazide (BSH). After preliminary separation by preparative HPLC,N-glycan-BSH components were de-labeled separately and reducingN-glycans were recovered. The de-labeled reducingN-glycans were derived with different labeling reagents and further separated and purified with two/multi-dimensional HPLC for various studies. We selected the bifunctional reagent 2-amino-N-(2-ami...
Source: Glycoconjugate Journal - February 5, 2020 Category: Biochemistry Source Type: research

Advanced glycosylation end products (AGEs) controls proliferation, invasion and permeability through orchestrating ARHGAP18/RhoA pathway in human umbilical vein endothelial cells
In conclusion, AGEs and ARHGAP18 orchestrate cell proliferation, invasion and permeability by contro lling the RhoA/ROCK signaling pathway, affecting NF-κB signaling pathway as well as the structure and morphology of VE-cadherin and tight junction protein, and regulating endothelial cell contraction. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - February 3, 2020 Category: Biochemistry Source Type: research

Expression, purification, and characterization of a new Glucosyltransferase involved in the third step of O-antigen repeating-unit biosynthesis of Escherichia coli O152
AbstractThe O antigen is indispensable for the full function and virulence of pathogenic bacteria. During O-repeating unit (RU) biosynthesis, committed glycosyltransferases (GTs) transfer various sugars from an activated sugar donor to the appropriate lipid carrier sequentially. While the nucleotide sequence specific for O antigen of pathogenic bacteria is already known, the exact substrate specificity of most hypothetical GTs have yet be characterized. In the present paper, we report the biochemical characterization of one alpha-glucosyltransferase, WfgE, a member of GT family 4. This enzyme is implicated in the pentasacc...
Source: Glycoconjugate Journal - January 23, 2020 Category: Biochemistry Source Type: research

The diagnostic value of serum DSA-TRF in hepatocellular carcinoma
AbstractTRF is a glycoprotein mainly secreted by hepatocytes, The aim of this study was to explore the diagnostic value of aberrant glycosylated serum transferrin (TRF) especially containing multi-antennary glycans in hepatocellular carcinoma (HCC).A total of 581 subjects including HCC patients, liver cirrhosis (LC) patients, chronic hepatitis (CHB) patients and healthy controls (HC) were recruited. All the subjects were randomly assigned to training group (n = 411) and validation group (n = 170). We firstly analyzed the serum protein N-glycome profiling of HCC, LC, and HC by DNA sequencer&ndash...
Source: Glycoconjugate Journal - January 13, 2020 Category: Biochemistry Source Type: research

Effects of extraction methods on physicochemical properties and hypoglycemic activities of polysaccharides from coarse green tea
This study aims to evaluate the effects of the extraction techniques WE (water extraction), UAE (ultrasound-assisted extraction), MAE (microwave-assisted extraction), and EE (enzyme extraction) on the physicochemical properties and antidiabetic activities of polysaccharides from coarse tea (CTPSs). The results showed that all four CTPSs had homogeneity in the monosaccharide types and similar IR (Infrared spectroscopy) characteristic absorption peaks, but differed in monosaccharide proportion and molecular weight distribution. Compared with the other three extraction techniques, CCTPS extracted by EE had the lowest protein ...
Source: Glycoconjugate Journal - January 8, 2020 Category: Biochemistry Source Type: research

Sclerotium rolfsii lectin induces opposite effects on normal PBMCs and leukemic Molt-4 cells by recognising TF antigen and its variants as receptors
AbstractSclerotiumrolfsii lectin (SRL) exerts apoptotic effect against various cancer cells and an antitumor activity on mice with colon and breast cancer xenografts. The current study aimed to explore its exquisite carbohydrate specificity on human peripheral blood mononuclear cells (PBMCs) and leukemic T-cells. SRL, showed strong binding (>98%) to resting/activated PBMCs, leukemic Molt-4 and Jurkat cell lines. The glycans mediated binding to these cells was effectively blocked by mucin and fetuin, exhibiting 97% and 94% inhibition respectively. SRL showed mitogenic stimulation of PBMCs at 10  μg/ml as determin...
Source: Glycoconjugate Journal - January 4, 2020 Category: Biochemistry Source Type: research

3 ′-sialyllactose targets cell surface protein, SIGLEC-3, and induces megakaryocyte differentiation and apoptosis by lipid raft-dependent endocytosis
In this study, we demonstrate that 3SL specifically binds to CD33. It induces megakaryocyte differentiation and subsequent apoptosis by targeting cell surface protein siglec-3 (CD33) in human chronic myeloid leukemia K562 cells. The 3SL-bound CD33 was internalized to the cyt osol via caveolae-dependent endocytosis. At the molecular level, 3SL-bound CD33 recruits the suppressor of cytokine signaling 3 (SOCS3) and SH2 domain-containing protein tyrosine phosphatase 1 (SHP1). SOCS3 is degraded with CD33 by proteasome degradation, while SHP-1 activates extracellular signal– regulated kinase (ERK) to induce megakaryocytic ...
Source: Glycoconjugate Journal - January 4, 2020 Category: Biochemistry Source Type: research

Structural analysis of glycosaminoglycans from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry
In this study, GAG fractions containing CS/DS and HA were isolated fromCCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography. The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths. The quantitative analysis first revealed that the amount of GAGs inCCA varied significantly in total and in each fraction. This novel struc...
Source: Glycoconjugate Journal - January 4, 2020 Category: Biochemistry Source Type: research

Changes in heparan sulfate sulfotransferases and cell-surface heparan sulfate during SKM-1 cells granulocytic differentiation and A549 cells epithelial-mesenchymal transition
AbstractHeparan sulfate (HS) with various sulfation patterns is one of important modulators of cancer cell fate through interacting with numerous growth factors. Here we found HS 2-O sulfotransferase 1 (HS2ST1) was downregulated at both mRNA and protein levels during granulocytic differentiation of SKM-1 leukemia cells and also HS (glucosamine) 3-O sulfotransferase 3A (HS3ST3A) in epithelial-mesenchymal transition (EMT) of A549 lung cancer cells, meanwhile, cell-surface HS recognized by anti-HS antibody was also changed in both cancer cell lines. Further, HS3ST3A was negatively correlated with thein vitro cell metastasis c...
Source: Glycoconjugate Journal - December 20, 2019 Category: Biochemistry Source Type: research

Glycan-binding profile of DC-like cells
In conclusion, not one of the studied cell lines proved an adequate model for DCs processes involving lectin binding. Although the glycan-binding profile of BYRB-Rb (8.17)1Iem mouse DCs could prove useful for assessing human DCs, important glycan interactions were missing, a situation which was aggravated when employing cells from the BALB/c strain. Accordingly, one must treat results from murine work with caution when seeking vaccine targeting of human DCs, and certainly should avoid cell lines such as THP-1 and DC2.4 cells. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - December 13, 2019 Category: Biochemistry Source Type: research

Interactions between Sclerostin and Glycosaminoglycans
AbstractSclerostin (SOST) is a glycoprotein having many important functions in the regulation of bone formation as a key negative regulator of Wnt signaling in bone. Surface plasmon resonance (SPR), which allows for a direct quantitative analysis of the label-free molecular interactions in real-time, has been widely used for the biophysical characterization of glycosaminoglycan (GAG)-protein interactions. In the present study, we report kinetics, structural analysis and the effects of physiological conditions (e.g., salt concentrations, Ca2+ and Zn2+concentrations) on the interactions between GAGs and recombinant human (rh...
Source: Glycoconjugate Journal - December 11, 2019 Category: Biochemistry Source Type: research

Chemical structures of oligosaccharides in milks of the American black bear ( Ursus americanus americanus ) and cheetah ( Acinonyx jubatus )
AbstractThe milk oligosaccharides were studied for two species of the Carnivora: the American black bear (Ursus americanus, family Ursidae, Caniformia), and the cheetah, (Acinonyx jubatus, family Felidae, Feliformia). Lactose was the most dominant saccharide in cheetah milk, while this was a minor saccharide and milk oligosaccharides predominated over lactose in American black bear milk. The structures of 8 neutral saccharides from American black bear milk were found to be Gal( β1–4)Glc (lactose), Fuc(α1–2)Gal(β1–4)Glc (2′-fucosyllactose), Gal(α1–3)Gal(β1–4)Glc...
Source: Glycoconjugate Journal - December 11, 2019 Category: Biochemistry Source Type: research

ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin
AbstractThe surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host ’s cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombina ntMusa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA,Lens culinaris lectin - LCA, Wheat germ agglutinin...
Source: Glycoconjugate Journal - December 10, 2019 Category: Biochemistry Source Type: research

Multifaceted antibodies development against synthetic α-dystroglycan mucin glycopeptide as promising tools for dystroglycanopathies diagnostic
AbstractDystroglycanopathies are diseases characterized by progressive muscular degeneration and impairment of patient ’s quality of life. They are associated with altered glycosylation of the dystrophin-glycoprotein (DGC) complex components, such as α-dystroglycan (α-DG), fundamental in the structural and functional stability of the muscle fiber. The diagnosis of dystroglycanopathies is currently based on the o bservation of clinical manifestations, muscle biopsies and enzymatic measures, and the available monoclonal antibodies are not specific for the dystrophic hypoglycosylated muscle condition. Thus, ...
Source: Glycoconjugate Journal - December 10, 2019 Category: Biochemistry Source Type: research

Relationship between heat treatment on structural properties and antitumor activity of the cold-water soluble polysaccharides from Grifola frondosa
AbstractGrifola frondosa is a basidiomycete fungus with potential biomedical applications owing to the presence of bioactive polysaccharides. The activities of polysaccharides are influenced by many factors, particularly temperature; however, the optimal temperature and conditions for preparation of polysaccharides from this organism have not yet been determined. Therefore, in this study, cold-water soluble polysaccharides fromGrifola frondosa were extracted at 4 °C (GFP-4) and purified. GFP-4-30, GFP-4-60 and GFP-4-90 were obtained from GFP-4 after treatment at 30 °C, 60 °C, or 90 °C, respectively, for 6 h...
Source: Glycoconjugate Journal - December 10, 2019 Category: Biochemistry Source Type: research

Investigation of substrate specificity of sialidases with membrane mimetic glycoconjugates
AbstractSialidases or neuraminidases play important roles in various physiological and pathological processes by cleaving terminal sialic acids (Sias) (desialylation) from the glycans of both glycoproteins and glycolipids. To understand the biological significance of desialylation by sialidases, it is important to investigate enzyme specificity with native substrate in biological membrane of cells. Herein, we report a membrane-mimicking system with liposome ganglioside conjugates containing different lipids for evaluating substrate specificity of sialidase and the lipid effect on the enzyme activity. Briefly, liposomes of ...
Source: Glycoconjugate Journal - December 4, 2019 Category: Biochemistry Source Type: research

Production and characterization of Aspergillus niger GH29 family α-fucosidase and production of a novel non-reducing 1-fucosyllactose
The objective of this study was to characterize the glycosyl hydrolase family 29 α-fucosidase produced byAspergillus niger and test its ability to transfucosylate lactose with a view towards potential industrial applications such as the valorization of the lactose side stream produced by dairy industry. In order to reduce costs and toxicity the use of free fucose instead of environmentally questionable fucose derivatives was studied. In contrast to earlier studies, a recombinantly producedA. nigerα-fucosidase was utilized. Using pNP-fucose as substrate, the optimal pH for hydrolytic activity was determined to b...
Source: Glycoconjugate Journal - December 2, 2019 Category: Biochemistry Source Type: research

Glycan structures and their recognition roles in the human blood group ABH/Ii, Le a, b, x, y and Sialyl Le a,x active cyst glycoproteins
AbstractHuman ovarian cyst glycoproteins (HOC, cyst gps) isolated from pseudomucinous type of human ovarian cyst fluids is one of the richest and pioneer sources for studying biosynthesis, structures and functional roles of blood groupABH,Lea,b,x,y,sLea andsLex active glycoproteins. After 70+ years of exploration, four top highlights are shared. (i) an updated concept of glycotopes and their internal structures in cyst gps was composited; (ii) the unknown codes of new genes in secreted cyst gps were unlocked asLex andLey; (iii) recognition profiles of cyst glycans and a sialic acid-rich (18%) glycan with lectins and antibo...
Source: Glycoconjugate Journal - November 26, 2019 Category: Biochemistry Source Type: research

Functional analysis of glycosylation using Drosophila melanogaster
AbstractThe glycosylation of proteins and lipids has various essential roles in a diverse range of biological processes, including embryogenesis, organ development, neurogenesis, maintenance of homeostasis, immune response, and tumorigenesis.Drosophila melanogaster is one of the representative multicellular model organisms, which have many useful genetic manipulation tools; it is used in developmental biology as well as classical and molecular genetics. Glycobiology is not an exception and many studies usingDrosophila have been performed in this field to clarify novel functions of glycans. Recently, genome-wide screening a...
Source: Glycoconjugate Journal - November 26, 2019 Category: Biochemistry Source Type: research

Maackia amurensis agglutinin induces apoptosis in cultured drug resistant human non-small cell lung cancer cells
In this study, the effect of MAA on drug resistant NSCLC cells was investigated. Two Paclitaxel-resistant NSCLC sub-lines (A549/PTX100 and NCI-H460/PTX100) were developed from A549& NCI-H460 cell lines respectively. The generation of drug resistance phenotype was confirmed by the expression of cell surface MDR-1. Both the drug resistant sub-lines showed distinct morphological alterations. MAA interacted with the cell-surface protein(s) of apparent Mr ~66  kDa and induced apoptosis in both the sub-lines through intrinsic/mitochondrial pathway, involving reduction in mitochondrial trans-membrane potential, up-regula...
Source: Glycoconjugate Journal - November 22, 2019 Category: Biochemistry Source Type: research

Glycan size and attachment site location affect electron transfer dissociation (ETD) fragmentation and automated glycopeptide identification
Abstract. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - October 21, 2019 Category: Biochemistry Source Type: research

ALG12-CDG: novel glycophenotype insights endorse the molecular defect
AbstractCongenital disorders of glycosylation (CDG) are genetic diseases characterized by deficient synthesis (CDG type I) and/or abnormal processing (CDG type II) of glycan moieties linked to protein and lipids. The impact of the molecular defects on protein glycosylation and in turn on the clinical phenotypes of patients with CDG is not yet understood. ALG12-CDG is due to deficiency of ALG12 α1,6-mannosyltransferase that adds the eighth mannose residue on the dolichol-PP-oligosaccharide precursor in the endoplasmic reticulum. ALG12-CDG is a severe multisystem disease associated with low to deficient serum immunoglo...
Source: Glycoconjugate Journal - September 16, 2019 Category: Biochemistry Source Type: research

Expression and localization of the small proteoglycans decorin and biglycan in articular cartilage of Kashin-Beck disease and rats induced by T-2 toxin and selenium deficiency
This study highlights the focal nature of the degenerative changes that occur in KBD cartilage and may suggest that the altered expression pattern of decorin and biglycan have an important role in the onset and pathogenesis of KBD. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - September 2, 2019 Category: Biochemistry Source Type: research

UDP- N -acetyl- α-D-galactosamine:polypeptide N -acetylgalactosaminyltransferase from the snail Biomphalaria glabrata – structural reflections
AbstractUDP-GalNAc:polypeptide GalNAc transferase (ppGalNAcT; EC is the initiating enzyme for mucin-type O-glycosylation in animals. Members of this highly conserved glycosyltransferase family catalyse a single glycosidic linkage. They transfer anN-acetylgalactosamine (GalNAc) residue from an activated donor (UDP-GalNAc) to a serine or threonine of an acceptor polypeptide chain. A ppGalNAcT from the freshwater snailBiomphalaria glabrata is the only characterised member of this enzyme family from mollusc origin. In this work, we interpret previously published experimental characterization of this enzyme in the con...
Source: Glycoconjugate Journal - August 8, 2019 Category: Biochemistry Source Type: research

Gangliosides profiling in serum of breast cancer patient: GM3 as a potential diagnostic biomarker
In conclusion, our results introduced the alteration patterns of serum gangliosides in breast cancer and suggested serum GM3 as a potential diagnostic biomarker in breast cancer diagnosis and luminal B subtype distinction. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - July 11, 2019 Category: Biochemistry Source Type: research

Glyco25, XXV International Symposium on Glycoconjugates
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - July 9, 2019 Category: Biochemistry Source Type: research

Anionic and zwitterionic moieties as widespread glycan modifications in non-vertebrates
AbstractGlycan structures in non-vertebrates are highly variable; it can be assumed that this is a product of evolution and speciation, not that it is just a random event. However, in animals and protists, there is a relatively limited repertoire of around ten monosaccharide building blocks, most of which are neutral in terms of charge. While two monosaccharide types in eukaryotes (hexuronic and sialic acids) are anionic, there are a number of organic or inorganic modifications of glycans such as sulphate, pyruvate, phosphate, phosphorylcholine, phosphoethanolamine and aminoethylphosphonate that also confer a ‘charge...
Source: Glycoconjugate Journal - July 5, 2019 Category: Biochemistry Source Type: research

Organocatalyzed preparation of 1,4,5-trisubstituted-glycosyl-1,2,3-triazole derivatives
AbstractOrganocatalytic coupling of glycosyl azides with enolates of active ketones and esters through azide-enolate [3  + 2] cycloaddition in the presence of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) furnished 1,4,5-trisubstituted-glycosyl-1,2,3-triazole derivatives in excellent yield. The reaction condition is simple and can be scaled-up.Graphical abstractCoupling of glycosyl azides with active ketones through azide-enolate [3  + 2] cycloaddition in the presence of 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU) furnished 1,4,5-trisubstituted-glycosyl-1,2,3-triazole derivatives in excellent yield. (So...
Source: Glycoconjugate Journal - July 5, 2019 Category: Biochemistry Source Type: research

Expanding our understanding of the role of microbial glycoproteomes through high-throughput mass spectrometry approaches
AbstractProtein glycosylation is increasingly recognised as an essential requirement for effective microbial infections. Within microbial pathogen ’s protein glycosylation is used for both defensive and offensive purposes; enabling pathogens to fortify themselves against the host immune response or to disarm the host’s ability to resist infection. Although microbial protein glycosylation systems have been recognised for nearly two decades only recently has the true extend of protein glycosylation within microbes begun to be appreciated. A key enabler for this conceptual shift has been the development and applic...
Source: Glycoconjugate Journal - July 4, 2019 Category: Biochemistry Source Type: research

“Stuck on sugars – how carbohydrates regulate cell adhesion, recognition, and signaling”
AbstractWe have explored the fundamental biological processes by which complex carbohydrates expressed on cellular glycoproteins and glycolipids and in secretions of cells promote cell adhesion and signaling. We have also explored processes by which animal pathogens, such as viruses, bacteria, and parasites adhere to glycans of animal cells and initiate disease. Glycans important in cell signaling and adhesion, such as key O-glycans, are essential for proper animal development and cellular differentiation, but they are also involved in many pathogenic processes, including inflammation, tumorigenesis and metastasis, and mic...
Source: Glycoconjugate Journal - July 2, 2019 Category: Biochemistry Source Type: research

The international Glycoconjugate organization awards
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - July 2, 2019 Category: Biochemistry Source Type: research

The International Glycoconjugate Organization Awards
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - July 2, 2019 Category: Biochemistry Source Type: research

Fluorine-modified sialyl-Tn-CRM197 vaccine elicits a robust immune response
In this study, we investigated the immunological activity of a fluoro-substituted Sialyl-Tn (F-STn) analogue coupled to the non-toxic cross-reactive material of diphtheria toxin197 (CRM197). Our results indicate that F-STn-CRM197 promotes a greater immunogenicity than non-fluorinated STn-CRM197. In the presence or absence of adjuvant, F-STn-CRM197 remarkably enhances both cellular and humoral immunity against STn by increasing antigen-specific lymphocyte proliferation and inducing a mixed Th1/Th2 response leading to production of IFN- γ and IL-4 cytokines, as well as STn-specific antibodies. Furthermore, antisera pro...
Source: Glycoconjugate Journal - July 2, 2019 Category: Biochemistry Source Type: research

Stage-specific embryonic antigen-4 is a histological marker reflecting the malignant behavior of prostate cancer
AbstractStage-specific embryonic antigen-4 (SSEA-4), a specific marker for pluripotent stem cells, plays an important role in the malignant behavior of several cancers. Here, SSEA-4 expression was evaluated by immunohistochemistry using monoclonal antibody RM1 specific to SSEA-4 in 181 and 117 prostate cancer (PC) specimens obtained by biopsy and radical prostatectomy (RP), respectively. The relationships between SSEA-4 expression in cancer cells or the presence of SSEA-4-positive tumor-infiltrating immune cells (TICs) and clinicopathological parameters were analyzed. SSEA-4 expression in cancer cells was significantly ass...
Source: Glycoconjugate Journal - June 26, 2019 Category: Biochemistry Source Type: research

Detection and characterization of bacterial polysaccharides in drug-resistant enterococci
In this study we explored the biological role of the capsule ofE. faecium. Our findings showed that the U0317 strain is not only sensitive to anti-LTA but also to antibodies against other enterococcal surface proteins. Our findings demonstrate that polysaccharides capsule mediated-resistance to opsonophagocytosis. We also found that the capsular polysaccharides do not play an important role in bacterial virulence in urinary tract and infective endocarditisin vivo models. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - June 22, 2019 Category: Biochemistry Source Type: research

Characterization of a novel glycolipid with a difucosylated H-antigen in human blood group O erythrocytes with monoclonal antibody HMMC-1 and its detection in human uterine cervical carcinoma tissues
AbstractHumanized monoclonal antibody HMMC-1 established by immunizing transchromosomal mice with a human uterine endometrial cancer cell line has been found to react with the H-antigen carried on core l O-glycans through cotransfection of glycosyltransferases for O-glycans and inhibition of antibody-binding with synthetic oligosaccharides. However, direct binding analysis of an antibody against glycosphingolipids from human erythrocytes with different ABO blood groups revealed that it was able to bind selectively with polar glycolipids in blood group O, but not blood group A, B and AB erythrocytes. Unexpectedly, typical m...
Source: Glycoconjugate Journal - May 16, 2019 Category: Biochemistry Source Type: research

A versatile salt-based method to immobilize glycosaminoglycans and create growth factor gradients
In conclusion, high concentrations of ammonium sulfate are effective for immobilization of GAGs and for the establishment of gradients of both GAGs and GAG-binding molecules, which enables the study to the biologic al roles of GAGs. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - May 4, 2019 Category: Biochemistry Source Type: research

Enzymatic synthesis of β -galactosyl fucose using recombinant bifidobacterial β -galactosidase and its prebiotic effect
In conclusion, a novel gal-fuc is expected to contribute to beneficial changes of gut microbiota.Graphical abstractA novel form ofβ-galactosyl fucose with an improved prebiotic effect. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - April 27, 2019 Category: Biochemistry Source Type: research

In vitro activation and maturation of human mononuclear phagocytes by stimulation with liposomes coated with a neoglycolipid containing α1–3, α1–6-mannotriose
In this study, we assessed the potential of liposomes coated with a neoglycolipid containing α1–3,α1–6-mannotriose residues (Man3-DPPE; Manα1–6(Manα1–3)Manitol-DPPE) forin vitro activation and maturation of human mononuclear phagocytes. In response to treatment with Man3-DPPE-coated liposomes (Man3-OMLs), PMA-stimulated human THP-1 cells showed enhanced expression of CD40, CD80 and HLA-DR and secreted significant levels of IL-12p40. Among various linkages of Man2-DPPE-coated liposomes, only liposomes coated with Man α1–6Manitol-DPPE (α1–6Man2-DPPE) ind...
Source: Glycoconjugate Journal - April 23, 2019 Category: Biochemistry Source Type: research