Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure
AbstractMethylene blue (MB) is one of the most common cationic dyes to detect heparin. As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated. It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding. The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number. For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acety...
Source: Glycoconjugate Journal - September 13, 2021 Category: Biochemistry Source Type: research
Degraded polysaccharides from Porphyra haitanensis: purification, physico-chemical properties, antioxidant and immunomodulatory activities
AbstractTo explore effect of the structural properties ofporphyra haitanensis polysaccharide on its biological activity, degradedporphyra polysaccharides were separated and purified by Cellulose DEAE-52 and Sephadex G-100 chromatography, obtaining three purified components (P1, P2 and P3). All the three components were sulfate polysaccharides containing the repeating units of → 3) β-D-galactose (1 → 4) 3,6-anhydro-α-L-galactose (1 →, and → 3) β-D-galactose (1 → 4) α-L-galactose-6-S (1 →, and → 3) 6-O-methyl-β-D-galactose (1 → 4) 3,6-anhydro-α-L-galactose (1 →. The mole...
Source: Glycoconjugate Journal - September 13, 2021 Category: Biochemistry Source Type: research
Development and Immunogenicity of a Brazilian Glycoconjugate vaccine against Meningococcal W in a Pilot Scale
In conclusion, this study established the best conditions to produce MenW conjugate bulks and showed the efficacy of the obta ined conjugate bulk in induce a good immune response in mice. Further experiments will need to be done to scale up the conjugation reaction and then allow the use of this conjugate in clinical trials. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - September 13, 2021 Category: Biochemistry Source Type: research
Binding ability of methylene blue with heparin dependent on its sulfate level rather than its sulfation location or basic saccharide structure
AbstractMethylene blue (MB) is one of the most common cationic dyes to detect heparin. As the sulfate residue presented in heparin was the main contributor to bind with MB, the UV performance of the MB with selectively desulfated heparin derivatives was investigated. It was found that the sulfate residue in different heparin analogues did not show the equal ability to attract MB binding. The stoichiometry of sulfate with MB among the heparin and derivatives was verified as a non-constant number. For the two selectively desulfated heparin derivatives: sulfate elimination at 6-O (6-OdeS) and N-acetylated heparin (N-deS-Acety...
Source: Glycoconjugate Journal - September 13, 2021 Category: Biochemistry Source Type: research
Degraded polysaccharides from Porphyra haitanensis: purification, physico-chemical properties, antioxidant and immunomodulatory activities
AbstractTo explore effect of the structural properties ofporphyra haitanensis polysaccharide on its biological activity, degradedporphyra polysaccharides were separated and purified by Cellulose DEAE-52 and Sephadex G-100 chromatography, obtaining three purified components (P1, P2 and P3). All the three components were sulfate polysaccharides containing the repeating units of → 3) β-D-galactose (1 → 4) 3,6-anhydro-α-L-galactose (1 →, and → 3) β-D-galactose (1 → 4) α-L-galactose-6-S (1 →, and → 3) 6-O-methyl-β-D-galactose (1 → 4) 3,6-anhydro-α-L-galactose (1 →. The mole...
Source: Glycoconjugate Journal - September 13, 2021 Category: Biochemistry Source Type: research
Development and Immunogenicity of a Brazilian Glycoconjugate vaccine against Meningococcal W in a Pilot Scale
In conclusion, this study established the best conditions to produce MenW conjugate bulks and showed the efficacy of the obta ined conjugate bulk in induce a good immune response in mice. Further experiments will need to be done to scale up the conjugation reaction and then allow the use of this conjugate in clinical trials. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - September 13, 2021 Category: Biochemistry Source Type: research
Effect of steaming process on the structural characteristics and antioxidant activities of polysaccharides from Polygonatum sibiricum rhizomes
In conclusion, our results proposed the fourth time as the optimal number of steaming to extract functional polysaccharide fromP. sibiricum rhizomes. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - September 8, 2021 Category: Biochemistry Source Type: research
Cross-reactivity of Haemophilus influenzae type a and b polysaccharides: molecular modeling and conjugate immunogenicity studies
AbstractHaemophilus influenzae is a leading cause of meningitis disease and mortality, particularly in young children. Since the introduction of a licensed conjugate vaccine (targeting the outer capsular polysaccharide) against the most prevalent serotype,Haemophilus influenzae serotype b, the epidemiology of the disease has changed andHaemophilus influenzae serotype a is on the rise, especially in Indigenous North American populations. Here we apply molecular modeling to explore the preferred conformations of the serotype a and b capsular polysaccharides as well as a modified hydrolysis resistant serotype b polysaccharide...
Source: Glycoconjugate Journal - September 7, 2021 Category: Biochemistry Source Type: research
Highly sialylated mucin-type glycopeptide from porcine intestinal mucosa after heparin extraction: O-glycan profiling and immunological activity evaluation
In this study, we isolated the mucins from the heparin extraction residues and profiled theO-glycans. After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography. The mucin derivedO-glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS). Thirty five kinds ofO-glycans were identified, most of which were Core 3-derived glycans. In particular, theO-glycans containing sialic acid Neu5Ac accounted for 71.93% of the totalO-glycans, which were ...
Source: Glycoconjugate Journal - September 4, 2021 Category: Biochemistry Source Type: research
35 years in plant lectin research: a journey from basic science to applications in agriculture and medicine
AbstractPlants contain an extended group of lectins differing from each other in their molecular structures, biochemical properties and carbohydrate-binding specificities. The heterogeneous group of plant lectins can be classified in several families based on the primary structure of the lectin domain. All proteins composed of one or more lectin domains, or having a domain architecture including one or more lectin domains in combination with other protein domains can be defined as lectins. Plant lectins reside in different cell compartments, and depending on their location will encounter a large variety carbohydrate struct...
Source: Glycoconjugate Journal - August 24, 2021 Category: Biochemistry Source Type: research
