Loci and motifs of the GalNAc α1 → 3/O related glycotopes in the mammalian glycoconjugates and their lectin recognition roles
AbstractGal α1 → andGalNAc α1 → are the two essential key sugars in human blood groupAB active glycotopes, in whichGalNAc α1 → related sequences are located at both sides of the nonreducing and the reducing ends of human blood groupA activeO-glycans. It is also found at the nonreducing ends of GlcNAcN-glycans and glycosphingolipid(GSL) of human blood groupA active glycotopes (Ah) and Forssman antigen (Fp). When monosaccharides and their α, β anomers are involved in basic units to express the complex size of the combining sites of theGalNAc α1 → specific lectins, they can be divided into a cavi...
Source: Glycoconjugate Journal - August 13, 2022 Category: Biochemistry Source Type: research
Structural determination of the lipid A from the deep-sea bacterium Zunongwangia profunda SM-A87: a small-scale approach
AbstractZunongwangia profunda SM-A87 is a deep-sea sedimentary bacterium from the phylum Bacteroidetes, representing a new genus ofFlavobacteriaceae. It was previously investigated for its capability of yielding high quantities of capsular polysaccharides (CPS) with interesting rheological properties, including high viscosity and tolerance to high salinities and temperatures. However, as a Gram-negative,Z. profunda SM-A87 also expresses lipopolysaccharides (LPS) as the main components of the external leaflet of its outer membrane. Here, we describe the isolation and characterization of the glycolipid part of this LPS, i.e....
Source: Glycoconjugate Journal - August 5, 2022 Category: Biochemistry Source Type: research
Roland Schauer – Obituary
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - August 3, 2022 Category: Biochemistry Source Type: research
Linking glycosphingolipids to Alzheimer ’s amyloid-ß: extracellular vesicles and functional plant materials
AbstractGlycosphingolipids (GSLs) are a specialized class of membrane lipids composed of a ceramide and a carbohydrate head group. GSLs are localized in cell membranes and were recently found to be enriched in the membrane of neuron-derived exosomes, which are a type of extracellular vesicle. Our studies demonstrated that exosomal GSLs may be associated with the amyloid- ß (Aß) peptide, a principal agent of Alzheimer’s disease (AD), and act to clear Aß by transporting Aß into brain phagocytic microglia. In this review, we summarize and discuss the function of exosomal GSLs in Aß homeostasis in AD pathology. Improvem...
Source: Glycoconjugate Journal - August 3, 2022 Category: Biochemistry Source Type: research
Roland Schauer – Obituary
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - August 3, 2022 Category: Biochemistry Source Type: research
Linking glycosphingolipids to Alzheimer ’s amyloid-ß: extracellular vesicles and functional plant materials
AbstractGlycosphingolipids (GSLs) are a specialized class of membrane lipids composed of a ceramide and a carbohydrate head group. GSLs are localized in cell membranes and were recently found to be enriched in the membrane of neuron-derived exosomes, which are a type of extracellular vesicle. Our studies demonstrated that exosomal GSLs may be associated with the amyloid- ß (Aß) peptide, a principal agent of Alzheimer’s disease (AD), and act to clear Aß by transporting Aß into brain phagocytic microglia. In this review, we summarize and discuss the function of exosomal GSLs in Aß homeostasis in AD pathology. Improvem...
Source: Glycoconjugate Journal - August 3, 2022 Category: Biochemistry Source Type: research
Studies on the partial characterization of extracted glycosaminoglycans from fish waste and its potentiality in modulating obesity through in-vitro and in-vivo
AbstractGlycosaminoglycans (GAGs) are bioactive polysaccharides or glycoconjugates found in the fish waste having significant health impacts. In the present study it has been attempted to extract GAGs from mackerel fish waste through chemical and enzymatic methods. Further, the extracted GAGs (e-GAGs) were analyzed for their composition (uronic acid, total sugar& sulfate), chemical characterization was carried out through techniques of scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR)& Proton NMR. Further, probable major GAGs present was identified by enzymatic digestion. The bio...
Source: Glycoconjugate Journal - August 1, 2022 Category: Biochemistry Source Type: research
Alteration of the neuronal and glial cell profiles in Neu1-deficient zebrafish
AbstractNeu1 is a glycosidase that releases sialic acids from the non-reducing ends of glycoconjugates, and its enzymatic properties are conserved among vertebrates. Recently, Neu1-KO zebrafish were generated using genome editing technology, and the KO fish showed abnormal emotional behavior, such as low schooling, low aggressiveness, and excess exploratory behavior, accompanied by the downregulation of anxiety-related genes. To examine the alteration of neuronal and glial cells in Neu1-KO zebrafish, we analyzed the molecular profiles in the zebrafish brain, focusing on the midbrain and telencephalon. Using immunohistochem...
Source: Glycoconjugate Journal - July 25, 2022 Category: Biochemistry Source Type: research
Science and the Russia-Ukraine War
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - July 20, 2022 Category: Biochemistry Source Type: research
Structure and physicochemical properties of polysaccharides from Poria cocos extracted by deep eutectic solvent
AbstractPoria cocos, a famous traditional Chinese medicine and a well-known food or food supplement, has shown therapeutic potential against cancer and the uneasiness of the mind. In addition, polysaccharides (PCPs) in this fungus were found to be various bioactive. In this work, one such PCP, PCP-1, extracted by deep eutectic solvent (DES) and separated using Sephadex G-15 columns, was characterized using GC –MS, HPGPC, FT-IR, and NMR, while also tested for physicochemical properties. Results indicated that PCP-1 contained 96.89 ± 3.21% total sugars and was a glucan with molecular weight of 3.2 kD. The main glycosi...
Source: Glycoconjugate Journal - July 16, 2022 Category: Biochemistry Source Type: research
