A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke
AbstractTheDalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin fromVataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires ...
Source: Glycoconjugate Journal - October 26, 2022 Category: Biochemistry Source Type: research
The story of the Sda antigen and of its cognate enzyme B4GALNT2: What is new?
AbstractThe structure Sia α2,3(GalNAcβ1,4)Gal- is the epitope of the Sda antigen, which is expressed on the erythrocytes and secretions of the vast majority of Caucasians, carried by N- and O-linked chains of glycoproteins, as well as by glycolipids. Sda is very similar, but not identical, to ganglioside GM2 [Sia α2,3(GalNAcβ1,4)Galβ1,4Glc-Cer]. The Sda synthase β1,4 N-acetylgalactosaminyl transferase 2 (B4GALNT2) exists in a short and a long form, diverging in the aminoterminal domain. The latter has a very long cytoplasmic tail and displays a Golgi- as well as a post-Golgi localization. The biosynthesis of Sda is ...
Source: Glycoconjugate Journal - October 26, 2022 Category: Biochemistry Source Type: research
A galactoside-specific Dalbergieae legume lectin from seeds of Vataireopsis araroba (Aguiar) Ducke
AbstractTheDalbergieae lectin group encompasses several lectins with significant differences in their carbohydrate specificities and biological properties. The current work reports on the purification and characterization of a GalNAc/Gal-specific lectin fromVataireopsis araroba (Aguiar) Ducke, designated as VaL. The lectin was purified from the seeds in a single step using guar gum affinity chromatography. The lectin migrated as a single band of about 35 kDa on SDS-PAGE and, in native conditions, occurs as a homodimer. The purified lectin is stable at temperatures up to 60 °C and in a pH range from 7 to 8 and requires ...
Source: Glycoconjugate Journal - October 26, 2022 Category: Biochemistry Source Type: research
Pseudomonas syringae DC3000 infection increases glucosylated N-glycans in Arabidopsis thaliana
AbstractStudying the interaction between the hemibiotrophic bacteriumPseudomonas syringae pv. tomato DC3000 andArabidopsis thaliana has shed light onto the various forms of mechanisms plants use to defend themselves against pathogen attack. While a lot of emphasis has been put on investigating changes in protein expression in infected plants, only little information is available on the effect infection plays on the plants N-glycan composition. To close this gap in knowledge, total N-glycans were enriched fromP. syringae DC3000-infected and mock treated Arabidopsis seedlings and analyzed via MALDI-TOF –MS. Additionally, f...
Source: Glycoconjugate Journal - October 21, 2022 Category: Biochemistry Source Type: research
Envelope Glycoprotein based multi-epitope vaccine against a co-infection of Human Herpesvirus 5 and Human Herpesvirus 6 using in silico strategies
In this study, an ensemble of viral envelope glycoproteins from both the viruses was utilized to design a multi-epitope vaccine using immunoinformatics tools. A collection of bacterial protein toll-like receptor agonists (BPTAs) was screened to identify a highly immunogenic adjuvant for the vaccine construct. The constructed vaccine was analysed using an array of methodologies ranging from World population coverage analysis to Immune simulation, whose results indicate high vaccine efficacy and stability. Furthermore, codon optimization andin silico cloning analysis were performed to check for efficient expression in a bact...
Source: Glycoconjugate Journal - October 13, 2022 Category: Biochemistry Source Type: research
6-O-desulfated heparin attenuates myocardial ischemia/reperfusion injury in mice through the regulation of miR-199a-5p/klotho axis
In conclusion, these results suggested that 6-O-desulfated heparin with lower anticoagulant activity attenuated myocardial I/R injury through miR-199a-5p/klotho and ROS signaling. Our study may also indicate that 6-O-desulfated heparin, as an excellent heparin derivative, is a potential therapeutic agent for myocardial I/R injury. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - September 15, 2022 Category: Biochemistry Source Type: research
Simple separation of glycosphingolipids in the lower phase of a Folch ’s partition from crude lipid fractions using zirconium dioxide
AbstractA simple method was developed for the separation of glycosphingolipids (GSLs) from lipid mixtures, including phospholipids and cholesterol, using zirconium dioxide (zirconia, ZrO2). Although this procedure does not incorporate a mild alkali treatment, which is commonly used for eliminating glycerophospholipids, it can be used to remove both alkali-resistant sphingomyelin and glycerophospholipids possessing ether bonds. Importantly, when GSLs were dissolved in organic solvent together with cholesterol (Chol) and phospholipids, and loaded onto ZrO2, Chol did not bind to the ZrO2 but both the GSLs and phospholipids di...
Source: Glycoconjugate Journal - September 14, 2022 Category: Biochemistry Source Type: research
Multicomponent reaction derived small di- and tri-carbohydrate-based glycomimetics as tools for probing lectin specificity
AbstractLectins, carbohydrate-binding proteins, play important functions in all forms of life from bacteria and viruses to plants, animals, and humans, participating in cell –cell communication and pathogen binding. In an attempt to modify lectin functions, artificial lectin ligands were made usually as big dendrimeric or cluster multivalent glycomimetic structures. Here we synthesized a novel set of glycomimetic ligands through protection/deprotection multicomponent reactions (MCR) approach. Multivalent di-and tri-carbohydrate glycomimetics containingd-fructose,d-galactose, andd-allose moieties were prepared in 63 –96...
Source: Glycoconjugate Journal - August 24, 2022 Category: Biochemistry Source Type: research
Proceedings of workshop: “Neuroglycoproteins in health and disease”, INNOGLY cost action
AbstractThe Cost Action “Innovation with glycans: new frontiers from synthesis to new biological targets” (INNOGLY) hosted the Workshop “Neuroglycoproteins in health and disease”, in Alicante, Spain, on March 2022. This event brought together an european group of scientists that presented novel insights into change s in glycosylation in diseases of the central nervous system and cancer, as well as new techniques to study protein glycosylation. Herein we provide the abstracts of all the presentations.Graphical Abstract (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - August 24, 2022 Category: Biochemistry Source Type: research
Multicomponent reaction derived small di- and tri-carbohydrate-based glycomimetics as tools for probing lectin specificity
AbstractLectins, carbohydrate-binding proteins, play important functions in all forms of life from bacteria and viruses to plants, animals, and humans, participating in cell –cell communication and pathogen binding. In an attempt to modify lectin functions, artificial lectin ligands were made usually as big dendrimeric or cluster multivalent glycomimetic structures. Here we synthesized a novel set of glycomimetic ligands through protection/deprotection multicomponent reactions (MCR) approach. Multivalent di-and tri-carbohydrate glycomimetics containingd-fructose,d-galactose, andd-allose moieties were prepared in 63 –96...
Source: Glycoconjugate Journal - August 24, 2022 Category: Biochemistry Source Type: research
Proceedings of workshop: “Neuroglycoproteins in health and disease”, INNOGLY cost action
AbstractThe Cost Action “Innovation with glycans: new frontiers from synthesis to new biological targets” (INNOGLY) hosted the Workshop “Neuroglycoproteins in health and disease”, in Alicante, Spain, on March 2022. This event brought together an european group of scientists that presented novel insights into change s in glycosylation in diseases of the central nervous system and cancer, as well as new techniques to study protein glycosylation. Herein we provide the abstracts of all the presentations.Graphical Abstract (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - August 24, 2022 Category: Biochemistry Source Type: research
