Correction to: Recognition factors of Dolichos biflorus agglutinin (DBA) and their accommodation sites
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - July 31, 2023 Category: Biochemistry Source Type: research
A glycomic workflow for LC –MS/MS analysis of urine glycosaminoglycan biomarkers in mucopolysaccharidoses
We describe a nano-LC –MS/MS method of GAG-NRE profiling, utilizing 2-aminobenzamide reductive amination labeling to improve the sensitivity and the chromatographic resolution. Diagnostic urinary GAG-NREs were identified for MPS types IH/IS, II, IIIc, IVa and VI, corroborating GAG-NRE as biomarkers for these known enzy me deficiencies. Furthermore, a significant reduction of diagnostic urinary GAG-NREs in MPS IH (n = 2) and MPS VI (n = 1) patients under treatment was demonstrated. We argue that this straightforward glycomic workflow, designed for the clinical analysis of MPS-related GAG-NREs in one single analysi...
Source: Glycoconjugate Journal - July 18, 2023 Category: Biochemistry Source Type: research
Characterization of pneumococcal serotype 7F in vaccine conjugation
AbstractStreptococcus pneumoniae is a highly invasive bacterial pathogen that can cause a range of illnesses. Pneumococcal capsular polysaccharides (CPS) are the main virulence factors that causes invasive pneumococcal disease (IPD). Pneumococcal CPS serotype 7F along with a few other serotypes is more invasive and likely to cause IPD. Therefore, 7F is a target for pneumococcal vaccine development, and is included in the two recently approved multi-valent pneumococcal conjugated vaccines, i.e. VAXNEUVANCE and PREVNAR 20.To support process and development of our 15-valent pneumococcal conjugated vaccine (PCV15), chromatogra...
Source: Glycoconjugate Journal - July 4, 2023 Category: Biochemistry Source Type: research
Mass photometry: A powerful tool for carbohydrates-proteins conjugation monitoring and glycoconjugates molecular mass determination
This study shows that the MP technique is a promising alternative to methods developed earlier f or monitoring glycoconjugation reactions and characterization of glycoconjugates. It measures intact molecules in solution and it is highly accurate over a wide mass range. MP requires only a very small amount of sample and has no specific buffer constraints. Other MP advantages include minimal cost of consumables and rapid data collection and analysis. Its advantages over other methods make it a valuable tool for researchers in the glycoconjugation field. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - July 1, 2023 Category: Biochemistry Source Type: research
Unravelling the genetic causality of immunoglobulin G N-glycans in ischemic stroke
AbstractBackground: Evidence suggests that immunoglobulin G (IgG) N-glycosylation is associated with ischemic stroke (IS). However, the causality of IgG N-glycosylation for IS remains unknown. Methods: Two-sample Mendelian randomization (MR) analyses were performed to investigate the potential causal effects of genetically determined IgG N-glycans on IS using publicly available summarized genetic data from East Asian and European populations. Genetic instruments were used as proxies for IgG N-glycan traits. IgG N-glycans were analysed using ultra-performance liquid chromatography. Four complementary MR methods were perform...
Source: Glycoconjugate Journal - June 21, 2023 Category: Biochemistry Source Type: research
Mucin glycans and their degradation by gut microbiota
AbstractThe human intestinal tract is inhabited by a tremendous number of microorganisms, which are collectively termed “the gut microbiota”. The intestinal epithelium is covered with a dense layer of mucus that prevents penetration of the gut microbiota into underlying tissues of the host. Recent studies have shown that the maturation and function of the mucus layer are strongly influenced by the gut microbiota, and alteration in the structure and function of the gut microbiota is implicated in several diseases. Because the intestinal mucus layer is at a crucial interface between microbes and their host, its breakdown...
Source: Glycoconjugate Journal - June 15, 2023 Category: Biochemistry Source Type: research
Recognition factors of Dolichos biflorus agglutinin (DBA) and their accommodation sites
In this study, many importnat recognition factors of DBA-glycan binding were characterized by our established enzyme-linked lectinosorbent (ELLSA) and inhibition assays. The results of these assays showed that the intensity profile of the recognition factors for the major combining site of DBA wa s expressed byMass relative potency (Mass R.P.) and shown by decreasing order of high density of polyvalent GalNAc α1 → (super glycotopes, 3.7 × 103) >> the corresponding β anomers >> monomeric GalNAcα1 → related glycotopes (GalNAc as 1.0) >> their GalNAc β-anomers >>...
Source: Glycoconjugate Journal - June 2, 2023 Category: Biochemistry Source Type: research
The generation of 5-N-glycolylneuraminic acid as a consequence of high levels of reactive oxygen species
In this study, the conversion ofN-acetylneuraminic acid (Neu5Ac) to Neu5Gc has been assessedin vitro under conditions mimicking the hydroxyl radical-rich humoral environment found in inflammatory and cancerous tissues. As a result, Neu5Gc has been detected via liquid chromatography-multiple reaction monitoring mass spectrometry. Furthermore, this conversion has also been found to take place in serum biomatrix containing ROS and in cancer cell cultures with induced ROS production. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - June 2, 2023 Category: Biochemistry Source Type: research
Recognition factors of Dolichos biflorus agglutinin (DBA) and their accommodation sites
In this study, many importnat recognition factors of DBA-glycan binding were characterized by our established enzyme-linked lectinosorbent (ELLSA) and inhibition assays. The results of these assays showed that the intensity profile of the recognition factors for the major combining site of DBA wa s expressed byMass relative potency (Mass R.P.) and shown by decreasing order of high density of polyvalent GalNAc α1 → (super glycotopes, 3.7 × 103) >> the corresponding β anomers >> monomeric GalNAcα1 → related glycotopes (GalNAc as 1.0) >> their GalNAc β-anomers >>...
Source: Glycoconjugate Journal - June 2, 2023 Category: Biochemistry Source Type: research
