ST3GalIV drives SLeX biosynthesis in gastrointestinal cancer cells and associates with cancer cell motility
In this study, we sought to elucidate the role of ST3GalIV in the biosynthesis of SLeX and in malignant properties of gastrointestinal (GI) cancer cells. By immunofluorescent screening, we selected SLeX-positive GI cancer cell lines and silenced ST3 GalIV expression via CRISPR/Cas9. Flow cytometry, immunofluorescence and western blot analysis showed that ST3GalIV KO efficiently impaired SLeX expression in most cancer cell lines, with the exception of the colon cancer cell line LS174T. The impact of ST3GalIV KO in the biosynthesis of SLeX isomer SLeA and non sialylated Lewis X and A were also evaluated and overall, ST3GalIV...
Source: Glycoconjugate Journal - April 19, 2023 Category: Biochemistry Source Type: research
Serum total carbohydrates, conjugated carbohydrates and total protein glycation index in diabetes mellitus
Conclusions: The results confirm that sTPGI obtained by indirect assay has high significant efficiency comparable to HbA1c to diagnose diabetes. sTPGI relative to HbA1c indicates the mean level of glycaemia over a shorter period of about one month so it responds more quickly to changes in therapy. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - April 15, 2023 Category: Biochemistry Source Type: research
Sialylation of cell surface glycoconjugates modulates cytosolic galectin-mediated responses upon organelle damage
AbstractSialylation is an important terminal modification of glycoconjugates that mediate diverse functions in physiology and disease. In this review we focus on how altered cell surface sialylation status is sensed by cytosolic galectins when the integrity of intracellular vesicles or organelles is compromised to expose luminal glycans to the cytosolic milieu, and how this impacts galectin-mediated cellular responses. In addition, we discuss the roles of mammalian sialidases on the cell surface, in the organelle lumen and cytosol, and raise the possibility that intracellular glycan processing may be critical in controllin...
Source: Glycoconjugate Journal - April 13, 2023 Category: Biochemistry Source Type: research
Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag
AbstractThe alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed. Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity. To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3...
Source: Glycoconjugate Journal - March 20, 2023 Category: Biochemistry Source Type: research
Vibrio-binding gangliosides in fish intestinal tracts
SummaryIt has been clarified that pathogens bind to glycosphingolipid (GSL) receptors in mammals, but there have been very few reports on pathogen-binding GSLs in fish. Vibrios are facultative anaerobic bacteria ubiquitous in marine and brackish environments. They are members of the normal intestinal microflora of healthy fish, but some species can cause a disease called vibriosis in fish and shellfish when the hosts are physiologically or immunologically weakened. The adherence of vibrios to host intestinal tracts is a significant event not only for survival and growth but also in terms of pathogenicity. We show in this m...
Source: Glycoconjugate Journal - March 18, 2023 Category: Biochemistry Source Type: research
Possible regulation of ganglioside GD3 synthase gene expression with DNA methylation in human glioma cells
In this study, we analyzed DNA methylation patterns of promoter regions ofGD3 synthase (ST8SIA1) as well as mRNA levels and ganglioside expression using human glioma cell lines. Among 5 cell lines examined, 4 lines showed changes in the expression levels of related genes after treatment with 5-aza-dC. LN319 showed up-regulation of St8sia1 and increased b-series gangliosides after 5-aza-dC treatment, and an astrocytoma cell line, AS showed high expression ofST8SIA1 and b-series gangliosides persistently before and after 5-Aza-2 ’-deoxycytidine treatment. Using these 2 cell lines, DNA methylation patterns of the promoter r...
Source: Glycoconjugate Journal - March 10, 2023 Category: Biochemistry Source Type: research
Simultaneous and sialic acid linkage-specific N- and O-linked glycan analysis by ester-to-amide derivatization
In this study, we developed a novel approach for sialic acid linkage-specificO-linked glycan analysis through lactone-driven ester-to-amide derivatization combined with non-reductive β-elimination in the presence of hydroxylamine.O-glycans released by non-reductive β-elimination were efficiently purified using glycoblotting via chemoselective ligation between carbohydrates and a hydrazide-functionalized polymer, followed by modification of methyl or ethyl ester groups of sialic acid residues on solid-phase. In-solution lactone-driven ester-to-amide derivatiza tion of ethyl-esterifiedO-glycans was performed, and the resul...
Source: Glycoconjugate Journal - March 6, 2023 Category: Biochemistry Source Type: research
Role of galectin-3 in vascular calcification
AbstractVascular calcification is an abnormal process in which bone specific hydroxyapatite crystals are actively deposited on the vascular wall mediated by phenotypic differentiated smooth muscle cells and other mesenchymal cells under various pathological conditions. It is one of the important characteristics in the occurrence and development of atherosclerosis, prevalent in patients with type 2 diabetes and advanced chronic kidney disease, especially those requiring maintenance hemodialysis, with severely threatening human health. Previous studies have shown that the early diagnosis and control of vascular calcification...
Source: Glycoconjugate Journal - February 20, 2023 Category: Biochemistry Source Type: research
