Phosphorylated chitosan accelerates dermal wound healing in diabetic wistar rats
AbstractPhosphorylated chitosan (PC), a water-soluble derivative of chitosan possesses several biological and chemical properties suitable for diabetic wound healing. In the present study, we report the synthesis and diabetic wound healing capabilities of PC. Elemental analysis, FT-IR,13C-NMR and31P-NMR techniques were employed for the chemical characterization of PC.In vitro, antioxidant properties of PC were determined in terms of Fe3+ reducing, metal chelating, lipid peroxidation and superoxide scavenging ability. The wound healing potential of PC was assessed in diabetic excisional wound rat model. PC exhibited good wa...
Source: Glycoconjugate Journal - November 30, 2022 Category: Biochemistry Source Type: research

Plant lectins as versatile tools to fight coronavirus outbreaks
AbstractThe S protein forming the homotrimeric spikes of pathogenic beta-coronaviruses, such as MERS-CoV, SARS-CoV and SARS-CoV-2, is a highly glycosylated protein containing mainlyN-glycans of the complex and high-mannose type, as well asO-glycans. Similarly, the host cell receptors DPP4 for MERS-CoV and ACE2 for SARS-CoV and SARS-CoV-2, also representN- andO-glycosylated proteins. All these glycoproteins share common glycosylation patterns, suggesting that plant lectins with different carbohydrate-binding specificities could be used as carbohydrate-binding agents for the spikes and their receptors, to combat COVID19 pand...
Source: Glycoconjugate Journal - November 24, 2022 Category: Biochemistry Source Type: research

Comparative study, homology modelling and molecular docking with cancer associated glycans of two non-fetuin-binding Tepary bean lectins
We present the purification and characterization of the two most abundant isoforms of lectins isolated from Tepary bean (Phaseolusacutifolius) seeds, which have been shown to differentially affect the survival of different cancer cells. They were separated by concanavalin A-affinity chromatography. After purification, to release theN-glycans, they were digested with the endoglycosidases PNGase and Glycanase A. Fractions resulted from the hydrolysis products were analyzed to determine their carbohydrate composition. Mass spectrometry data indicated that both isoforms contained high mannose glycans being mannose 6 the most a...
Source: Glycoconjugate Journal - November 17, 2022 Category: Biochemistry Source Type: research

Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD
AbstractThe pH value was essential for the growth and metabolism of microorganisms. Acidic pH exopolysaccharide (AC-EPS) and alkaline pH exopolysaccharide (AL-EPS) secreted byA. australica QD mediated by pH were studied in this paper. The total carbohydrate content and molecular weight of AC-EPS (79.59%  ± 2.24% (w/w), 8.374 × 105 Da) and AL-EPS (82.48% ± 1.46% (w/w), 6.182 × 105 Da) were estimated and compared. In AC-EPS, mannose (3.78%) and galactose (3.24%) content was more, while the proportion of glucuronic acid was less in comparison to AL-EPS. The scanning electron microscopy revealed the struc...
Source: Glycoconjugate Journal - November 11, 2022 Category: Biochemistry Source Type: research

A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo
In this study, a water-soluble polysaccharide fromEucommia folium was extracted by hot water and purified using Sephadex G-200 gel columns. The results showed that the purified fraction (EFP) has a molecular weight of 9.98  × 105 Da and consisted of rhamnose, arabinose, galactose, glucose, mannose, xylose, galacturonic acid, and glucuronic acid (molar ratio: 0.226: 1.739: 2.183: 1: 0.155: 0.321: 0.358: 0.047). The combination of infrared spectroscopy and NMR analysis proved that EFP is an acidic polysaccharide whose main c hain consists of α-L-Araf-(1  → , → 3,5)-α-Araf-(1  → , → 3)-β-Galp-...
Source: Glycoconjugate Journal - November 7, 2022 Category: Biochemistry Source Type: research

Afterword (Editorial)
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - November 2, 2022 Category: Biochemistry Source Type: research

Glycoproteomic analysis reveals the effects of bisecting GlcNAc in intrahepatic cholangiocarcinoma
This study sheds light on the potential effects of bisecting GlcNAc in ICC cells and suggests thatMGAT3 might be used as a potential target in the therapy of ICC. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - November 2, 2022 Category: Biochemistry Source Type: research

Afterword (Editorial)
(Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - November 2, 2022 Category: Biochemistry Source Type: research

Glycoproteomic analysis reveals the effects of bisecting GlcNAc in intrahepatic cholangiocarcinoma
This study sheds light on the potential effects of bisecting GlcNAc in ICC cells and suggests thatMGAT3 might be used as a potential target in the therapy of ICC. (Source: Glycoconjugate Journal)
Source: Glycoconjugate Journal - November 2, 2022 Category: Biochemistry Source Type: research