Structural characterization and biological activities of a new polysaccharide isolated from Morchella Sextelata

In this study, a new water-soluble polysaccharide (MSP-3-1) with an average molecular weight of 2.35 × 107 Da was extracted and purified from fruiting bodies of cultivatedM. Sextelata. The structural characterization and biological activities of purified polysaccharide was further investigated. The results indicated that MSP-3-1 was mainly a α-glucan, mainly consisting of mannose (Man), glucose (Glc) and galactose (Gal) in a ratio of 5.10: 91.39: 3.51. Its surface morphology exhibited irregular lamellar structures with small voids. And the particle size analysis showed that MSP-3-1 was the homogeneous nanoparticle in water solution. Fu rthermore, the antioxidant activity analysis showed that MSP-3-1 possessed certain scavenging activity against hydroxyl radicals, DPPH radicals and ABTS radicals in a dose-dependent manner. Immunological tests suggested that MSP-3-1 could significantly promote the proliferation, phagocytosis and nit ric oxide (NO) production of macrophage RAW264.7. Thus, our results will provide a theoretical basis for the development and utilization ofMorchella Sextelata polysaccharides as an immunmodulatory component in functional foods.
Source: Glycoconjugate Journal - Category: Biochemistry Source Type: research