Reference genes for gene expression profiling in mouse models of < em > Listeria monocytogenes < /em > infection
This study prospected RGs for mouse macrophages (pMØ) and spleen infected with Listeria monocytogenes. The pMØ were infected in vitro with L. monocytogenes or vehicle for 4 h. Mice were injected with L. monocytogenes (or vehicle) and euthanized 24 h post-injection. The RGs came from a multispecies primer set, from the literature or designed here. The RG ranking relied on GeNorm, NormFinder, BestKeeper, Delta-CT and RefFinder. B2m-H3f3a-Ppia were the most stable RGs for pMØ, albeit RG indexes fine-tuned estimations of cytokine relative expression. Actβ-Ubc-Ppia were the best RGs for spleen but modestly impacted the cyto...
Source: BioTechniques - December 19, 2023 Category: Biotechnology Authors: Lethicia Souza Tavares Roberta Lane Oliveira-Silva Marcelo Tigre Moura J éssica Barboza da Silva Ana Maria Benko-Iseppon Jos é Vitor Lima-Filho Source Type: research
Reference genes for gene expression profiling in mouse models of < em > Listeria monocytogenes < /em > infection
This study prospected RGs for mouse macrophages (pMØ) and spleen infected with Listeria monocytogenes. The pMØ were infected in vitro with L. monocytogenes or vehicle for 4 h. Mice were injected with L. monocytogenes (or vehicle) and euthanized 24 h post-injection. The RGs came from a multispecies primer set, from the literature or designed here. The RG ranking relied on GeNorm, NormFinder, BestKeeper, Delta-CT and RefFinder. B2m-H3f3a-Ppia were the most stable RGs for pMØ, albeit RG indexes fine-tuned estimations of cytokine relative expression. Actβ-Ubc-Ppia were the best RGs for spleen but modestly impacted the cyto...
Source: BioTechniques - December 19, 2023 Category: Biotechnology Authors: Lethicia Souza Tavares Roberta Lane Oliveira-Silva Marcelo Tigre Moura J éssica Barboza da Silva Ana Maria Benko-Iseppon Jos é Vitor Lima-Filho Source Type: research
Is space the final frontier for mitochondrial study?
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0071. Online ahead of print.ABSTRACTTweetable abstract This perspective considers several avenues for future research on mitochondrial dynamics, stress, and DNA in outer space.PMID:38084381 | DOI:10.2144/btn-2023-0071 (Source: BioTechniques)
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Kit Neikirk Dominique C Stephens Heather K Beasley Andrea G Marshall Jennifer A Gaddy Steven M Damo Antentor Hinton Source Type: research
Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0052. Online ahead of print.ABSTRACTAdditive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines - HEK 293 (kidney) and K7M2 wt (bone) - were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended perio...
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Derek C DeMel Grayson A Wagner Jamie A Maresca John P Geibel Source Type: research
Is space the final frontier for mitochondrial study?
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0071. Online ahead of print.ABSTRACTTweetable abstract This perspective considers several avenues for future research on mitochondrial dynamics, stress, and DNA in outer space.PMID:38084381 | DOI:10.2144/btn-2023-0071 (Source: BioTechniques)
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Kit Neikirk Dominique C Stephens Heather K Beasley Andrea G Marshall Jennifer A Gaddy Steven M Damo Antentor Hinton Source Type: research
Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0052. Online ahead of print.ABSTRACTAdditive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines - HEK 293 (kidney) and K7M2 wt (bone) - were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended perio...
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Derek C DeMel Grayson A Wagner Jamie A Maresca John P Geibel Source Type: research
Is space the final frontier for mitochondrial study?
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0071. Online ahead of print.ABSTRACTTweetable abstract This perspective considers several avenues for future research on mitochondrial dynamics, stress, and DNA in outer space.PMID:38084381 | DOI:10.2144/btn-2023-0071 (Source: BioTechniques)
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Kit Neikirk Dominique C Stephens Heather K Beasley Andrea G Marshall Jennifer A Gaddy Steven M Damo Antentor Hinton Source Type: research
Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0052. Online ahead of print.ABSTRACTAdditive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines - HEK 293 (kidney) and K7M2 wt (bone) - were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended perio...
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Derek C DeMel Grayson A Wagner Jamie A Maresca John P Geibel Source Type: research
Is space the final frontier for mitochondrial study?
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0071. Online ahead of print.ABSTRACTTweetable abstract This perspective considers several avenues for future research on mitochondrial dynamics, stress, and DNA in outer space.PMID:38084381 | DOI:10.2144/btn-2023-0071 (Source: BioTechniques)
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Kit Neikirk Dominique C Stephens Heather K Beasley Andrea G Marshall Jennifer A Gaddy Steven M Damo Antentor Hinton Source Type: research
Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0052. Online ahead of print.ABSTRACTAdditive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines - HEK 293 (kidney) and K7M2 wt (bone) - were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended perio...
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Derek C DeMel Grayson A Wagner Jamie A Maresca John P Geibel Source Type: research
Is space the final frontier for mitochondrial study?
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0071. Online ahead of print.ABSTRACTTweetable abstract This perspective considers several avenues for future research on mitochondrial dynamics, stress, and DNA in outer space.PMID:38084381 | DOI:10.2144/btn-2023-0071 (Source: BioTechniques)
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Kit Neikirk Dominique C Stephens Heather K Beasley Andrea G Marshall Jennifer A Gaddy Steven M Damo Antentor Hinton Source Type: research
Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0052. Online ahead of print.ABSTRACTAdditive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines - HEK 293 (kidney) and K7M2 wt (bone) - were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended perio...
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Derek C DeMel Grayson A Wagner Jamie A Maresca John P Geibel Source Type: research
Is space the final frontier for mitochondrial study?
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0071. Online ahead of print.ABSTRACTTweetable abstract This perspective considers several avenues for future research on mitochondrial dynamics, stress, and DNA in outer space.PMID:38084381 | DOI:10.2144/btn-2023-0071 (Source: BioTechniques)
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Kit Neikirk Dominique C Stephens Heather K Beasley Andrea G Marshall Jennifer A Gaddy Steven M Damo Antentor Hinton Source Type: research
Application of a 3D bioprinter: jet technology for 'biopatch' development using cells on hydrogel supports
Biotechniques. 2023 Dec 12. doi: 10.2144/btn-2023-0052. Online ahead of print.ABSTRACTAdditive manufacturing (3D printing) has been deployed across multiple platforms to fabricate bioengineered tissues. We demonstrate the use of a Thermal Inkjet Pipette System (TIPS) for targeted delivery of cells onto manufactured substrates to design bio-bandages. Two cell lines - HEK 293 (kidney) and K7M2 wt (bone) - were applied using TIPS. We demonstrate a novel means for targeted cell delivery to a hydrogel support structure. These cell/support constructs (bio-bandages) had a high viability for survival and growth over extended perio...
Source: BioTechniques - December 12, 2023 Category: Biotechnology Authors: Derek C DeMel Grayson A Wagner Jamie A Maresca John P Geibel Source Type: research
Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide
Conclusion: The H2S quantification method should be selected based on the specific requirements of a research project in terms of sensitivity, response time and cost-effectiveness.PMID:38059376 | DOI:10.2144/btn-2023-0075 (Source: BioTechniques)
Source: BioTechniques - December 7, 2023 Category: Biotechnology Authors: Susmit Mhatre Anjali Rai Hatim Ali Akash Patil Neetu Singh Richa Verma John Auden Cole Chandler Alekha Dash Catherine Opere Somnath Singh Source Type: research
