Special Focus Issue - Celebrating 40 years of publishing lab techniques: looking back to look foreword
Biotechniques. 2023 Sep;75(3):75-77. doi: 10.2144/btn-2023-0081.NO ABSTRACTPMID:37724394 | DOI:10.2144/btn-2023-0081 (Source: BioTechniques)
Source: BioTechniques - September 19, 2023 Category: Biotechnology Authors: Beatrice Bowlby Tristan Free Annie Coulson Aisha Al-Janabi Ashling Cannon Ebony Torrington Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep;75(3):115-128. doi: 10.2144/btn-2023-0023. Epub 2023 Sep 8.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome pr...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep 8. doi: 10.2144/btn-2023-0023. Online ahead of print.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep 8. doi: 10.2144/btn-2023-0023. Online ahead of print.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep 8. doi: 10.2144/btn-2023-0023. Online ahead of print.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep 8. doi: 10.2144/btn-2023-0023. Online ahead of print.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep 8. doi: 10.2144/btn-2023-0023. Online ahead of print.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep 8. doi: 10.2144/btn-2023-0023. Online ahead of print.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A practical approach to genome assembly and annotation of Basidiomycota using the example of < em > Armillaria < /em >
Biotechniques. 2023 Sep 8. doi: 10.2144/btn-2023-0023. Online ahead of print.ABSTRACTTechnological advancements in genome sequencing, assembly and annotation platforms and algorithms that resulted in several genomic studies have created an opportunity to further our understanding of the biology of phytopathogens, including Armillaria species. Most Armillaria species are facultative necrotrophs that cause root- and stem-rot, usually on woody plants, significantly impacting agriculture and forestry worldwide. Genome sequencing, assembly and annotation in terms of samples used and methods applied in Armillaria genome projects...
Source: BioTechniques - September 8, 2023 Category: Biotechnology Authors: Deborah L Narh Mensah Brenda D Wingfield Martin Pa Coetzee Source Type: research
A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines
Biotechniques. 2023 Sep 6. doi: 10.2144/btn-2023-0026. Online ahead of print.ABSTRACTRecombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.PMID:37671637 | DOI:10.2144/btn-2023...
Source: BioTechniques - September 6, 2023 Category: Biotechnology Authors: Yu Peng Chun'e Zhang Minyan Deng Haijuan Jiang Huishu Huang Yue Li Weiping Lai Yu-Pin Lin Jun Yu Source Type: research
A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines
Biotechniques. 2023 Sep 6. doi: 10.2144/btn-2023-0026. Online ahead of print.ABSTRACTRecombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.PMID:37671637 | DOI:10.2144/btn-2023...
Source: BioTechniques - September 6, 2023 Category: Biotechnology Authors: Yu Peng Chun'e Zhang Minyan Deng Haijuan Jiang Huishu Huang Yue Li Weiping Lai Yu-Pin Lin Jun Yu Source Type: research
A cell hybridization-based method of generating recombinant rabbit monoclonal antibodies for detecting cytokines
Biotechniques. 2023 Sep 6. doi: 10.2144/btn-2023-0026. Online ahead of print.ABSTRACTRecombinant rabbit monoclonal antibodies (rabbit rAbs) have shown promise in various biomedical fields. However, it is challenging and costly to generate rabbit rAbs using traditional techniques. Here we describe a convenient and cost-effective method. Using this method, we generated rabbit rAbs against mouse soluble IL-6 receptor α with affinities in the range of 10-9 to 10-12 M. The presented method is suitable for industrial and academic scientists looking to customize rabbit rAbs for their research.PMID:37671637 | DOI:10.2144/btn-2023...
Source: BioTechniques - September 6, 2023 Category: Biotechnology Authors: Yu Peng Chun'e Zhang Minyan Deng Haijuan Jiang Huishu Huang Yue Li Weiping Lai Yu-Pin Lin Jun Yu Source Type: research
Education in microscopy: taking a closer look with Jennifer Waters
Biotechniques. 2023 Aug 29. doi: 10.2144/btn-2023-0079. Online ahead of print.ABSTRACTJennifer Waters (Nikon Imaging Center, Harvard Medical School, MA, USA) spoke to Ebony Torrington (Future Science Group, London, UK) about her career in microscopy and the importance of education. Jennifer is the Director of the Nikon Imaging Center (NIC) at Harvard Medical School and a Chan Zuckerberg Initiative (CZI) Imaging Scientist. She was trained in cell biology and microscopy by Ted Salmon at University of North Carolina (UNC) at Chapel Hill (NC, USA), where her research focused on mitosis and cell cycle regulation. Jennifer's mai...
Source: BioTechniques - August 29, 2023 Category: Biotechnology Authors: Jennifer Waters Source Type: research
Education in microscopy: taking a closer look with Jennifer Waters
Biotechniques. 2023 Aug 29. doi: 10.2144/btn-2023-0079. Online ahead of print.ABSTRACTJennifer Waters (Nikon Imaging Center, Harvard Medical School, MA, USA) spoke to Ebony Torrington (Future Science Group, London, UK) about her career in microscopy and the importance of education. Jennifer is the Director of the Nikon Imaging Center (NIC) at Harvard Medical School and a Chan Zuckerberg Initiative (CZI) Imaging Scientist. She was trained in cell biology and microscopy by Ted Salmon at University of North Carolina (UNC) at Chapel Hill (NC, USA), where her research focused on mitosis and cell cycle regulation. Jennifer's mai...
Source: BioTechniques - August 29, 2023 Category: Biotechnology Authors: Jennifer Waters Source Type: research
Education in microscopy: taking a closer look with Jennifer Waters
Biotechniques. 2023 Aug 29. doi: 10.2144/btn-2023-0079. Online ahead of print.ABSTRACTJennifer Waters (Nikon Imaging Center, Harvard Medical School, MA, USA) spoke to Ebony Torrington (Future Science Group, London, UK) about her career in microscopy and the importance of education. Jennifer is the Director of the Nikon Imaging Center (NIC) at Harvard Medical School and a Chan Zuckerberg Initiative (CZI) Imaging Scientist. She was trained in cell biology and microscopy by Ted Salmon at University of North Carolina (UNC) at Chapel Hill (NC, USA), where her research focused on mitosis and cell cycle regulation. Jennifer's mai...
Source: BioTechniques - August 29, 2023 Category: Biotechnology Authors: Jennifer Waters Source Type: research
