An easy method for quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins
In this study, the authors highlight the errors incurred by using fluorescent proteins under limited oxygenation by comparing standard fluorescence-based reporter gene assays to quantitative real-time PCR data in the study of a complex oxygen-regulated gene network. Furthermore, a solution to perform quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins using a microplate reader with an oxygen control system and applying pulses of full oxygenation before fluorescence measurements is provided.PMID:37880975 | DOI:10.2144/btn-2023-0064 (Source: BioTechniques)
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Lucas Pedraz Eduard Torrents Source Type: research
A simple, sensitive and colorimetric assay for < em > Pseudomonas aeruginosa < /em > infection analysis
Biotechniques. 2023 Oct 26. doi: 10.2144/btn-2023-0030. Online ahead of print.ABSTRACTSkin and soft tissue infections caused by Pseudomonas aeruginosa are common acquired diseases in postpartum care. Many methods have been developed in recent years for detecting P. aeruginosa, but they are criticized for the drawbacks of labor-intensiveness, complicated operation and high cost. Here, a simple, sensitive and colorimetric assay for P. aeruginosa detection is described. The approach displays a green color for positive samples and colorless for target-free samples. The approach exhibits a wide detection range and a low limit o...
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Jie Liu Dan Lu Junyuan Wang Source Type: research
A simple and rapid method for isolating high-quality RNA from kenaf with high polysaccharide and polyphenol contents
Biotechniques. 2023 Oct 26. doi: 10.2144/btn-2023-0077. Online ahead of print.ABSTRACTThe extraction of high-quality RNA from kenaf is essential for genetic and molecular biology research. However, the presence of high levels of polysaccharide and polyphenol compounds in kenaf poses challenges for RNA isolation. We proposed a simplified, time-saving and cost-effective method for isolating high quantities of RNA from various kenaf tissues. This method exhibited superior efficiency in RNA isolation compared with the conventional cetyltrimethylammonium bromide method and demonstrated greater adaptability to different samples ...
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Xiaofang Liao Yanhong Zhao Hongwei Li Wenhuan Hou Xingfu Tang Ruiyang Zhou Source Type: research
An easy method for quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins
In this study, the authors highlight the errors incurred by using fluorescent proteins under limited oxygenation by comparing standard fluorescence-based reporter gene assays to quantitative real-time PCR data in the study of a complex oxygen-regulated gene network. Furthermore, a solution to perform quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins using a microplate reader with an oxygen control system and applying pulses of full oxygenation before fluorescence measurements is provided.PMID:37880975 | DOI:10.2144/btn-2023-0064 (Source: BioTechniques)
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Lucas Pedraz Eduard Torrents Source Type: research
A simple, sensitive and colorimetric assay for < em > Pseudomonas aeruginosa < /em > infection analysis
Biotechniques. 2023 Oct 26. doi: 10.2144/btn-2023-0030. Online ahead of print.ABSTRACTSkin and soft tissue infections caused by Pseudomonas aeruginosa are common acquired diseases in postpartum care. Many methods have been developed in recent years for detecting P. aeruginosa, but they are criticized for the drawbacks of labor-intensiveness, complicated operation and high cost. Here, a simple, sensitive and colorimetric assay for P. aeruginosa detection is described. The approach displays a green color for positive samples and colorless for target-free samples. The approach exhibits a wide detection range and a low limit o...
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Jie Liu Dan Lu Junyuan Wang Source Type: research
A simple and rapid method for isolating high-quality RNA from kenaf with high polysaccharide and polyphenol contents
Biotechniques. 2023 Oct 26. doi: 10.2144/btn-2023-0077. Online ahead of print.ABSTRACTThe extraction of high-quality RNA from kenaf is essential for genetic and molecular biology research. However, the presence of high levels of polysaccharide and polyphenol compounds in kenaf poses challenges for RNA isolation. We proposed a simplified, time-saving and cost-effective method for isolating high quantities of RNA from various kenaf tissues. This method exhibited superior efficiency in RNA isolation compared with the conventional cetyltrimethylammonium bromide method and demonstrated greater adaptability to different samples ...
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Xiaofang Liao Yanhong Zhao Hongwei Li Wenhuan Hou Xingfu Tang Ruiyang Zhou Source Type: research
An easy method for quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins
In this study, the authors highlight the errors incurred by using fluorescent proteins under limited oxygenation by comparing standard fluorescence-based reporter gene assays to quantitative real-time PCR data in the study of a complex oxygen-regulated gene network. Furthermore, a solution to perform quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins using a microplate reader with an oxygen control system and applying pulses of full oxygenation before fluorescence measurements is provided.PMID:37880975 | DOI:10.2144/btn-2023-0064 (Source: BioTechniques)
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Lucas Pedraz Eduard Torrents Source Type: research
A simple, sensitive and colorimetric assay for < em > Pseudomonas aeruginosa < /em > infection analysis
Biotechniques. 2023 Oct 26. doi: 10.2144/btn-2023-0030. Online ahead of print.ABSTRACTSkin and soft tissue infections caused by Pseudomonas aeruginosa are common acquired diseases in postpartum care. Many methods have been developed in recent years for detecting P. aeruginosa, but they are criticized for the drawbacks of labor-intensiveness, complicated operation and high cost. Here, a simple, sensitive and colorimetric assay for P. aeruginosa detection is described. The approach displays a green color for positive samples and colorless for target-free samples. The approach exhibits a wide detection range and a low limit o...
Source: BioTechniques - October 26, 2023 Category: Biotechnology Authors: Jie Liu Dan Lu Junyuan Wang Source Type: research
Decoding the mechanisms of embryo development with spatial biology
Biotechniques. 2023 Oct 19. doi: 10.2144/btn-2023-0093. Online ahead of print.ABSTRACT[Formula: see text] Spatial genomics first emerged in the 1990s to map biological function data onto multidimensional images of embryo specimens and has continued to be instrumental in studying embryogenesis and understanding early human development in the decades since.PMID:37855245 | DOI:10.2144/btn-2023-0093 (Source: BioTechniques)
Source: BioTechniques - October 19, 2023 Category: Biotechnology Authors: Aisha Al-Janabi Source Type: research
Decoding the mechanisms of embryo development with spatial biology
Biotechniques. 2023 Oct 19. doi: 10.2144/btn-2023-0093. Online ahead of print.ABSTRACT[Formula: see text] Spatial genomics first emerged in the 1990s to map biological function data onto multidimensional images of embryo specimens and has continued to be instrumental in studying embryogenesis and understanding early human development in the decades since.PMID:37855245 | DOI:10.2144/btn-2023-0093 (Source: BioTechniques)
Source: BioTechniques - October 19, 2023 Category: Biotechnology Authors: Aisha Al-Janabi Source Type: research
Decoding the mechanisms of embryo development with spatial biology
Biotechniques. 2023 Oct 19. doi: 10.2144/btn-2023-0093. Online ahead of print.ABSTRACT[Formula: see text] Spatial genomics first emerged in the 1990s to map biological function data onto multidimensional images of embryo specimens and has continued to be instrumental in studying embryogenesis and understanding early human development in the decades since.PMID:37855245 | DOI:10.2144/btn-2023-0093 (Source: BioTechniques)
Source: BioTechniques - October 19, 2023 Category: Biotechnology Authors: Aisha Al-Janabi Source Type: research
Decoding the mechanisms of embryo development with spatial biology
Biotechniques. 2023 Oct 19. doi: 10.2144/btn-2023-0093. Online ahead of print.ABSTRACT[Formula: see text] Spatial genomics first emerged in the 1990s to map biological function data onto multidimensional images of embryo specimens and has continued to be instrumental in studying embryogenesis and understanding early human development in the decades since.PMID:37855245 | DOI:10.2144/btn-2023-0093 (Source: BioTechniques)
Source: BioTechniques - October 19, 2023 Category: Biotechnology Authors: Aisha Al-Janabi Source Type: research
Decoding the mechanisms of embryo development with spatial biology
Biotechniques. 2023 Oct 19. doi: 10.2144/btn-2023-0093. Online ahead of print.ABSTRACT[Formula: see text] Spatial genomics first emerged in the 1990s to map biological function data onto multidimensional images of embryo specimens and has continued to be instrumental in studying embryogenesis and understanding early human development in the decades since.PMID:37855245 | DOI:10.2144/btn-2023-0093 (Source: BioTechniques)
Source: BioTechniques - October 19, 2023 Category: Biotechnology Authors: Asisha Al-Janabi Source Type: research
Decoding the mechanisms of embryo development with spatial biology
Biotechniques. 2023 Oct 19. doi: 10.2144/btn-2023-0093. Online ahead of print.ABSTRACT[Formula: see text] Spatial genomics first emerged in the 1990s to map biological function data onto multidimensional images of embryo specimens and has continued to be instrumental in studying embryogenesis and understanding early human development in the decades since.PMID:37855245 | DOI:10.2144/btn-2023-0093 (Source: BioTechniques)
Source: BioTechniques - October 19, 2023 Category: Biotechnology Authors: Asisha Al-Janabi Source Type: research
A simplified and rapid < em > in situ < /em > hybridization protocol for planarians
Biotechniques. 2023 Oct 18. doi: 10.2144/btn-2023-0074. Online ahead of print.ABSTRACTWhole-mount in situ hybridization is a critical technique for analyzing gene expression in planarians. While robust in situ protocols have been developed, these protocols are laborious, making them challenging to incorporate in an academic setting, reducing throughput and increasing time to results. Here, the authors systematically tested modifications to all phases of the protocol with the goal of eliminating steps and reducing time without impacting quality. This modified protocol allows for whole-mount colorimetric in situ hybridizatio...
Source: BioTechniques - October 18, 2023 Category: Biotechnology Authors: Andrew J Gaetano Ryan S King Source Type: research
