Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.PMID:38131320 | DOI:10.2144/btn-2023-0104 (Source: BioTechniques)
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Source Type: research
An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis
Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that t...
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Yingchun Ma Bowen Li Dejia Yang Shuguang Wang Lixia Yu Hui Zhan Juan Li Source Type: research
Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.PMID:38131320 | DOI:10.2144/btn-2023-0104 (Source: BioTechniques)
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Source Type: research
An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis
Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that t...
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Yingchun Ma Bowen Li Dejia Yang Shuguang Wang Lixia Yu Hui Zhan Juan Li Source Type: research
Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.PMID:38131320 | DOI:10.2144/btn-2023-0104 (Source: BioTechniques)
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Source Type: research
An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis
Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that t...
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Yingchun Ma Bowen Li Dejia Yang Shuguang Wang Lixia Yu Hui Zhan Juan Li Source Type: research
Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.PMID:38131320 | DOI:10.2144/btn-2023-0104 (Source: BioTechniques)
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Source Type: research
An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis
Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that t...
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Yingchun Ma Bowen Li Dejia Yang Shuguang Wang Lixia Yu Hui Zhan Juan Li Source Type: research
Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.PMID:38131320 | DOI:10.2144/btn-2023-0104 (Source: BioTechniques)
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Source Type: research
An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis
Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that t...
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Yingchun Ma Bowen Li Dejia Yang Shuguang Wang Lixia Yu Hui Zhan Juan Li Source Type: research
Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.PMID:38131320 | DOI:10.2144/btn-2023-0104 (Source: BioTechniques)
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Source Type: research
An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis
Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that t...
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Yingchun Ma Bowen Li Dejia Yang Shuguang Wang Lixia Yu Hui Zhan Juan Li Source Type: research
Assessment of measurement accuracy of amplified DNA using a colorimetric loop-mediated isothermal amplification assay
In this study, we evaluated amplified DNA concentrations measured using the colorimetric LAMP assay of the 1POT™ Professional device (1drop Inc, Korea). Results of the 1POT analysis of clinical samples were compared with measurements obtained from the Qubit™ 4 and NanoDrop™ 2000 devices (both from Thermo Fisher Scientific, MA, USA). These results showed a correlation of 0.98 (95% CI: 0.96-0.99) and 0.96 (95% CI: 0.92-0.98) between 1POT and the Qubit and NanoDrop. 1POT can measure amplified DNA accurately and is suitable for on-site molecular diagnostics.PMID:38131320 | DOI:10.2144/btn-2023-0104 (Source: BioTechniques)
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Seongsoo Hong Jeongho Park Jaekyung Bok Euna Cho Joowon Rhee Source Type: research
An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis
Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that t...
Source: BioTechniques - December 22, 2023 Category: Biotechnology Authors: Yingchun Ma Bowen Li Dejia Yang Shuguang Wang Lixia Yu Hui Zhan Juan Li Source Type: research
Reference genes for gene expression profiling in mouse models of < em > Listeria monocytogenes < /em > infection
This study prospected RGs for mouse macrophages (pMØ) and spleen infected with Listeria monocytogenes. The pMØ were infected in vitro with L. monocytogenes or vehicle for 4 h. Mice were injected with L. monocytogenes (or vehicle) and euthanized 24 h post-injection. The RGs came from a multispecies primer set, from the literature or designed here. The RG ranking relied on GeNorm, NormFinder, BestKeeper, Delta-CT and RefFinder. B2m-H3f3a-Ppia were the most stable RGs for pMØ, albeit RG indexes fine-tuned estimations of cytokine relative expression. Actβ-Ubc-Ppia were the best RGs for spleen but modestly impacted the cyto...
Source: BioTechniques - December 19, 2023 Category: Biotechnology Authors: Lethicia Souza Tavares Roberta Lane Oliveira-Silva Marcelo Tigre Moura J éssica Barboza da Silva Ana Maria Benko-Iseppon Jos é Vitor Lima-Filho Source Type: research
