An optimal genomic DNA extraction method for shoots of four < em > Dendrocalamus < /em > species based on membership function analysis

Biotechniques. 2023 Dec 22. doi: 10.2144/btn-2023-0087. Online ahead of print.ABSTRACTHigh-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that the commercial DNA extraction kit method was the most effective and comprehensive approach for extracting genomic DNA from shoots of four Dendrocalamus species. Furthermore, this study provided valuable insights into optimizing techniques for extracting genomic DNA in other bamboo species.PMID:38131324 | DOI:10.2144/btn-2023-0087
Source: BioTechniques - Category: Biotechnology Authors: Source Type: research