Generation of < em > UL128 < /em > -shRNA transduced fibroblasts for the release of cell-free virus from clinical human cytomegalovirus isolates
In conclusion, UL128-shRNA-transduced fibroblasts allow applications previously unsuitable for recent isolates.PMID:37846844 | DOI:10.2144/btn-2023-0046 (Source: BioTechniques)
Source: BioTechniques - October 17, 2023 Category: Biotechnology Authors: Nina Weiler Kerstin Laib Sampaio Myriam Scherer Christian Sinzger Source Type: research
Generation of < em > UL128 < /em > -shRNA transduced fibroblasts for the release of cell-free virus from clinical human cytomegalovirus isolates
In conclusion, UL128-shRNA-transduced fibroblasts allow applications previously unsuitable for recent isolates.PMID:37846844 | DOI:10.2144/btn-2023-0046 (Source: BioTechniques)
Source: BioTechniques - October 17, 2023 Category: Biotechnology Authors: Nina Weiler Kerstin Laib Sampaio Myriam Scherer Christian Sinzger Source Type: research
PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs
Biotechniques. 2023 Oct;75(4):168-178. doi: 10.2144/btn-2023-0042. Epub 2023 Oct 10.ABSTRACTWith advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current cloning platforms fall short regarding affordability, ease of combinatorial assembly and, above all, the ability to iteratively remove individual cassettes at will. Herein we construct, implement and make available a broad set of cloning vectors, called PlayBack vectors, that allow ...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Gregory Foran Ryan D Hallam Marvel Megaly Anel Turgambayeva Aleksandar Necakov Source Type: research
Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol
Biotechniques. 2023 Oct;75(4):157-167. doi: 10.2144/btn-2023-0017. Epub 2023 Oct 10.ABSTRACTSingle-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we d...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Firoozeh V Gerayeli Stephen Milne Chen Xi Yang Xuan Li Elizabeth Guinto Julia Shun-Wei Yang Chung Yan Cheung Tara R Stach Tawimas Shaipanich Janice M Leung Don D Sin Source Type: research
PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs
Biotechniques. 2023 Oct;75(4):168-178. doi: 10.2144/btn-2023-0042. Epub 2023 Oct 10.ABSTRACTWith advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current cloning platforms fall short regarding affordability, ease of combinatorial assembly and, above all, the ability to iteratively remove individual cassettes at will. Herein we construct, implement and make available a broad set of cloning vectors, called PlayBack vectors, that allow ...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Gregory Foran Ryan D Hallam Marvel Megaly Anel Turgambayeva Aleksandar Necakov Source Type: research
Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol
Biotechniques. 2023 Oct;75(4):157-167. doi: 10.2144/btn-2023-0017. Epub 2023 Oct 10.ABSTRACTSingle-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we d...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Firoozeh V Gerayeli Stephen Milne Chen Xi Yang Xuan Li Elizabeth Guinto Julia Shun-Wei Yang Chung Yan Cheung Tara R Stach Tawimas Shaipanich Janice M Leung Don D Sin Source Type: research
PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs
Biotechniques. 2023 Oct;75(4):168-178. doi: 10.2144/btn-2023-0042. Epub 2023 Oct 10.ABSTRACTWith advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current cloning platforms fall short regarding affordability, ease of combinatorial assembly and, above all, the ability to iteratively remove individual cassettes at will. Herein we construct, implement and make available a broad set of cloning vectors, called PlayBack vectors, that allow ...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Gregory Foran Ryan D Hallam Marvel Megaly Anel Turgambayeva Aleksandar Necakov Source Type: research
Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol
Biotechniques. 2023 Oct;75(4):157-167. doi: 10.2144/btn-2023-0017. Epub 2023 Oct 10.ABSTRACTSingle-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we d...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Firoozeh V Gerayeli Stephen Milne Chen Xi Yang Xuan Li Elizabeth Guinto Julia Shun-Wei Yang Chung Yan Cheung Tara R Stach Tawimas Shaipanich Janice M Leung Don D Sin Source Type: research
PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs
Biotechniques. 2023 Oct;75(4):168-178. doi: 10.2144/btn-2023-0042. Epub 2023 Oct 10.ABSTRACTWith advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current cloning platforms fall short regarding affordability, ease of combinatorial assembly and, above all, the ability to iteratively remove individual cassettes at will. Herein we construct, implement and make available a broad set of cloning vectors, called PlayBack vectors, that allow ...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Gregory Foran Ryan D Hallam Marvel Megaly Anel Turgambayeva Aleksandar Necakov Source Type: research
Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol
Biotechniques. 2023 Oct;75(4):157-167. doi: 10.2144/btn-2023-0017. Epub 2023 Oct 10.ABSTRACTSingle-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we d...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Firoozeh V Gerayeli Stephen Milne Chen Xi Yang Xuan Li Elizabeth Guinto Julia Shun-Wei Yang Chung Yan Cheung Tara R Stach Tawimas Shaipanich Janice M Leung Don D Sin Source Type: research
PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs
Biotechniques. 2023 Oct 10. doi: 10.2144/btn-2023-0042. Online ahead of print.ABSTRACTWith advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current cloning platforms fall short regarding affordability, ease of combinatorial assembly and, above all, the ability to iteratively remove individual cassettes at will. Herein we construct, implement and make available a broad set of cloning vectors, called PlayBack vectors, that allow for th...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Gregory Foran Ryan D Hallam Marvel Megaly Anel Turgambayeva Aleksandar Necakov Source Type: research
Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol
Biotechniques. 2023 Oct 10. doi: 10.2144/btn-2023-0017. Online ahead of print.ABSTRACTSingle-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we describ...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Firoozeh V Gerayeli Stephen Milne Chen Xi Yang Xuan Li Elizabeth Guinto Julia Shun-Wei Yang Chung Yan Cheung Tara R Stach Tawimas Shaipanich Janice M Leung Don D Sin Source Type: research
PlayBack cloning: simple, reversible, cost-effective cloning for the combinatorial assembly of complex expression constructs
Biotechniques. 2023 Oct 10. doi: 10.2144/btn-2023-0042. Online ahead of print.ABSTRACTWith advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current cloning platforms fall short regarding affordability, ease of combinatorial assembly and, above all, the ability to iteratively remove individual cassettes at will. Herein we construct, implement and make available a broad set of cloning vectors, called PlayBack vectors, that allow for th...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Gregory Foran Ryan D Hallam Marvel Megaly Anel Turgambayeva Aleksandar Necakov Source Type: research
Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol
Biotechniques. 2023 Oct 10. doi: 10.2144/btn-2023-0017. Online ahead of print.ABSTRACTSingle-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we describ...
Source: BioTechniques - October 10, 2023 Category: Biotechnology Authors: Firoozeh V Gerayeli Stephen Milne Chen Xi Yang Xuan Li Elizabeth Guinto Julia Shun-Wei Yang Chung Yan Cheung Tara R Stach Tawimas Shaipanich Janice M Leung Don D Sin Source Type: research
Microbial forensics: what we've learned from Amerithrax and beyond
Biotechniques. 2023 Oct 6. doi: 10.2144/btn-2023-0084. Online ahead of print.ABSTRACTThe Amerithrax investigation into anthrax letter attacks in the USA forever changed the game in microbial forensics. Here we review the techniques used, then and now, to neutralize bioterrorism threats. [Formula: see text].PMID:37800360 | DOI:10.2144/btn-2023-0084 (Source: BioTechniques)
Source: BioTechniques - October 6, 2023 Category: Biotechnology Authors: Louis Gautier Source Type: research
