A free and user-friendly software protocol for the quantification of microfauna swimming behavior
Biotechniques. 2024 Feb 29. doi: 10.2144/btn-2024-0001. Online ahead of print.ABSTRACTCharacterizing swimming behavior can provide a holistic assessment of the health, physiology and ecology of microfaunal species when done in conjunction with measuring other biological parameters. However, tracking and quantifying microfauna swimming behavior using existing automated tools is often difficult due to the animals' small size or transparency, or because of the high cost, expertise, or labor needed for the analysis. To address these issues, we created a cost-effective, user-friendly protocol for behavior analysis that employs ...
Source: BioTechniques - March 1, 2024 Category: Biotechnology Authors: Colleen M McMaken Kristin E Gribble Source Type: research
Multiparameter screen optimizes immunoprecipitation
Biotechniques. 2024 Feb 29. doi: 10.2144/btn-2023-0051. Online ahead of print.ABSTRACTImmunoprecipitation (IP) coupled with mass spectrometry effectively maps protein-protein interactions when genome-wide, affinity-tagged cell collections are used. Such studies have recorded significant portions of the compositions of physiological protein complexes, providing draft 'interactomes'; yet many constituents of protein complexes still remain uncharted. This gap exists partly because high-throughput approaches cannot optimize each IP. A key challenge for IP optimization is stabilizing in vivo interactions during the transfer fro...
Source: BioTechniques - March 1, 2024 Category: Biotechnology Authors: Shaoshuai Xie Leila Saba Hua Jiang Omar R Bringas Mehrnoosh Oghbaie Luciano Di Stefano Vadim Sherman John LaCava Source Type: research
A free and user-friendly software protocol for the quantification of microfauna swimming behavior
Biotechniques. 2024 Feb 29. doi: 10.2144/btn-2024-0001. Online ahead of print.ABSTRACTCharacterizing swimming behavior can provide a holistic assessment of the health, physiology and ecology of microfaunal species when done in conjunction with measuring other biological parameters. However, tracking and quantifying microfauna swimming behavior using existing automated tools is often difficult due to the animals' small size or transparency, or because of the high cost, expertise, or labor needed for the analysis. To address these issues, we created a cost-effective, user-friendly protocol for behavior analysis that employs ...
Source: BioTechniques - March 1, 2024 Category: Biotechnology Authors: Colleen M McMaken Kristin E Gribble Source Type: research
Multiparameter screen optimizes immunoprecipitation
Biotechniques. 2024 Feb 29. doi: 10.2144/btn-2023-0051. Online ahead of print.ABSTRACTImmunoprecipitation (IP) coupled with mass spectrometry effectively maps protein-protein interactions when genome-wide, affinity-tagged cell collections are used. Such studies have recorded significant portions of the compositions of physiological protein complexes, providing draft 'interactomes'; yet many constituents of protein complexes still remain uncharted. This gap exists partly because high-throughput approaches cannot optimize each IP. A key challenge for IP optimization is stabilizing in vivo interactions during the transfer fro...
Source: BioTechniques - March 1, 2024 Category: Biotechnology Authors: Shaoshuai Xie Leila Saba Hua Jiang Omar R Bringas Mehrnoosh Oghbaie Luciano Di Stefano Vadim Sherman John LaCava Source Type: research
Considerations for developing mitochondrial transplantation techniques for individualized medicine
Biotechniques. 2024 Feb 29. doi: 10.2144/btn-2023-0072. Online ahead of print.ABSTRACTTweetable abstract Mitochondrial transplantation has been used to treat various diseases associated with mitochondrial dysfunction. Here, we highlight the considerations in quality control mechanisms that should be considered in the context of mitochondrial transplantation.PMID:38420889 | DOI:10.2144/btn-2023-0072 (Source: BioTechniques)
Source: BioTechniques - February 29, 2024 Category: Biotechnology Authors: Kit Neikirk Dominique C Stephens Heather K Beasley Andrea G Marshall Jennifer A Gaddy Steven M Damo Antentor O Hinton Source Type: research
Comparing robotic and manual injection methods in zebrafish embryos for high-throughput RNA silencing using CRISPR-RfxCas13d
In this study, the authors compared the efficiency of automated robotic and manual injection methods for the CRISPR-RfxCas13d (CasRx) system for mRNA knockdown and Cas9-mediated DNA targeting in zebrafish embryos. They targeted the no tail (TBXTA) gene as a proof-of-principle, evaluating the induced embryonic phenotypes. Both Cas9 and CasRx systems caused loss of function phenotypes for TBXTA. Cas9 protein exhibited a higher percentage of severe phenotypes compared with mRNA, while CasRx protein and mRNA showed similar efficiency. Both robotic and manual injections demonstrated comparable phenotype percentages and mortalit...
Source: BioTechniques - February 29, 2024 Category: Biotechnology Authors: Joaquin Abugattas-Nu ñez Del Prado Yi Ding Jan de Sonneville Kees-Jan van der Kolk Miguel A Moreno-Mateos Edward M álaga-Trillo Herman P Spaink Source Type: research
Corrigendum
Biotechniques. 2024 Feb 28. doi: 10.2144/btn-2020-0153c1. Online ahead of print.NO ABSTRACTPMID:38415630 | DOI:10.2144/btn-2020-0153c1 (Source: BioTechniques)
Source: BioTechniques - February 28, 2024 Category: Biotechnology Source Type: research
Corrigendum
Biotechniques. 2024 Feb 28. doi: 10.2144/btn-2022-0032c1. Online ahead of print.NO ABSTRACTPMID:38415670 | DOI:10.2144/btn-2022-0032c1 (Source: BioTechniques)
Source: BioTechniques - February 28, 2024 Category: Biotechnology Source Type: research
Corrigendum
Biotechniques. 2024 Feb 28. doi: 10.2144/btn-2020-0153c1. Online ahead of print.NO ABSTRACTPMID:38415630 | DOI:10.2144/btn-2020-0153c1 (Source: BioTechniques)
Source: BioTechniques - February 28, 2024 Category: Biotechnology Source Type: research
Corrigendum
Biotechniques. 2024 Feb 28. doi: 10.2144/btn-2022-0032c1. Online ahead of print.NO ABSTRACTPMID:38415670 | DOI:10.2144/btn-2022-0032c1 (Source: BioTechniques)
Source: BioTechniques - February 28, 2024 Category: Biotechnology Source Type: research
Age is just a number: can proteomic analysis provide insight into the aging body?
Biotechniques. 2024 Feb 22. doi: 10.2144/btn-2024-0008. Online ahead of print.ABSTRACTRecent proteomic studies have increased our understanding of the molecular process of aging, potentially enabling age-related diseases to be treated before the appearance of symptoms. [Formula: see text].PMID:38386391 | DOI:10.2144/btn-2024-0008 (Source: BioTechniques)
Source: BioTechniques - February 22, 2024 Category: Biotechnology Authors: Jenny Straiton Source Type: research
Age is just a number: can proteomic analysis provide insight into the aging body?
Biotechniques. 2024 Feb 22. doi: 10.2144/btn-2024-0008. Online ahead of print.ABSTRACTRecent proteomic studies have increased our understanding of the molecular process of aging, potentially enabling age-related diseases to be treated before the appearance of symptoms. [Formula: see text].PMID:38386391 | DOI:10.2144/btn-2024-0008 (Source: BioTechniques)
Source: BioTechniques - February 22, 2024 Category: Biotechnology Authors: Jenny Straiton Source Type: research
Age is just a number: can proteomic analysis provide insight into the aging body?
Biotechniques. 2024 Feb 22. doi: 10.2144/btn-2024-0008. Online ahead of print.ABSTRACTRecent proteomic studies have increased our understanding of the molecular process of aging, potentially enabling age-related diseases to be treated before the appearance of symptoms. [Formula: see text].PMID:38386391 | DOI:10.2144/btn-2024-0008 (Source: BioTechniques)
Source: BioTechniques - February 22, 2024 Category: Biotechnology Authors: Jenny Straiton Source Type: research
Age is just a number: can proteomic analysis provide insight into the aging body?
Biotechniques. 2024 Feb 22. doi: 10.2144/btn-2024-0008. Online ahead of print.ABSTRACTRecent proteomic studies have increased our understanding of the molecular process of aging, potentially enabling age-related diseases to be treated before the appearance of symptoms. [Formula: see text].PMID:38386391 | DOI:10.2144/btn-2024-0008 (Source: BioTechniques)
Source: BioTechniques - February 22, 2024 Category: Biotechnology Authors: Jenny Straiton Source Type: research
Age is just a number: can proteomic analysis provide insight into the aging body?
Biotechniques. 2024 Feb 22. doi: 10.2144/btn-2024-0008. Online ahead of print.ABSTRACTRecent proteomic studies have increased our understanding of the molecular process of aging, potentially enabling age-related diseases to be treated before the appearance of symptoms. [Formula: see text].PMID:38386391 | DOI:10.2144/btn-2024-0008 (Source: BioTechniques)
Source: BioTechniques - February 22, 2024 Category: Biotechnology Authors: Jenny Straiton Source Type: research
