Quantitative analysis of lncRNA in formalin-fixed paraffin-embedded tissues of oral squamous cell carcinoma
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0033. Online ahead of print.ABSTRACTThe study evaluated expression profiles of few regulatory lncRNAs in oral squamous cell carcinoma and normal mucosa adjacent to oral cancer using paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues stored at a different duration of time (1-5 years) using real-time quantitative PCR. The quantity and quality of total RNA isolated from FFPE tissues was less compared with that of fresh frozen tissues, which resulted in a noncorrelation of quantification cycle values. Following normalization, the expression of lncRNAs in th...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Kiran Kumar Kaveri Hallikeri Ajaykumar Oli Mallikarjun Goni Apoorva Jain Jagadeesha Poyya Alagilavada S Shilpasree Palaksha Kanive Javaregowda Source Type: research
A high-throughput pipeline for DNA/RNA/small RNA purification from tissue samples for sequencing
Biotechniques. 2023 Aug;75(2):47-55. doi: 10.2144/btn-2023-0011. Epub 2023 Aug 8.ABSTRACTHigh-throughput total nucleic acid (TNA) purification methods based on solid-phase reversible immobilization (SPRI) beads produce TNA suitable for both genomic and transcriptomic applications. Even so, small RNA species, including miRNA, bind weakly to SPRI beads under standard TNA purification conditions, necessitating a separate workflow using column-based methods that are difficult to automate. Here, an SPRI-based high-throughput TNA purification protocol that recovers DNA, RNA and small RNA, called GSC-modified RLT+ Aline bead-base...
Source: BioTechniques - August 8, 2023 Category: Biotechnology Authors: Jing Xu Pawan K Pandoh Richard D Corbett Duane Smailus Reanne Bowlby Denise Brooks Helen McDonald Simon Haile Sundeep Chahal Steve Bilobram Karen L Mungall Andrew J Mungall Robin Coope Richard A Moore Yongjun Zhao Steven Jm Jones Marco A Marra Source Type: research
Selective expansion of target cells using the Enrich TROVO platform
Biotechniques. 2023 Aug;75(2):56-64. doi: 10.2144/btn-2023-0038. Epub 2023 Aug 8.ABSTRACTEnriching target cell clones from diverse cell populations is vital for many life science applications. We have developed a novel method to rapidly and efficiently purify specific clonal cell populations from a larger, heterogeneous group using the Enrich TroVo system (Enrich Biosystems Inc., CT, USA). This system takes advantage of microfabrication and optical technologies by utilizing small hydrogel wells to separate desired cell populations and an innovative patching technique to selectively eliminate undesired cells. This method al...
Source: BioTechniques - August 8, 2023 Category: Biotechnology Authors: Prem Shrestha Stephen Rotarori Kirby Madden-Hennessey Christina Mohammed Chi-Han Yang Jordan Urbani Joseph Pettinelli Xueqi Liu Qi Zhao Source Type: research
A high-throughput pipeline for DNA/RNA/small RNA purification from tissue samples for sequencing
Biotechniques. 2023 Aug 8. doi: 10.2144/btn-2023-0011. Online ahead of print.ABSTRACTHigh-throughput total nucleic acid (TNA) purification methods based on solid-phase reversible immobilization (SPRI) beads produce TNA suitable for both genomic and transcriptomic applications. Even so, small RNA species, including miRNA, bind weakly to SPRI beads under standard TNA purification conditions, necessitating a separate workflow using column-based methods that are difficult to automate. Here, an SPRI-based high-throughput TNA purification protocol that recovers DNA, RNA and small RNA, called GSC-modified RLT+ Aline bead-based pr...
Source: BioTechniques - August 8, 2023 Category: Biotechnology Authors: Jing Xu Pawan K Pandoh Richard D Corbett Duane Smailus Reanne Bowlby Denise Brooks Helen McDonald Simon Haile Sundeep Chahal Steve Bilobram Karen L Mungall Andrew J Mungall Robin Coope Richard A Moore Yongjun Zhao Steven Jm Jones Marco A Marra Source Type: research
Selective expansion of target cells using the Enrich TROVO platform
Biotechniques. 2023 Aug 8. doi: 10.2144/btn-2023-0038. Online ahead of print.ABSTRACTEnriching target cell clones from diverse cell populations is vital for many life science applications. We have developed a novel method to rapidly and efficiently purify specific clonal cell populations from a larger, heterogeneous group using the Enrich TroVo system (Enrich Biosystems Inc., CT, USA). This system takes advantage of microfabrication and optical technologies by utilizing small hydrogel wells to separate desired cell populations and an innovative patching technique to selectively eliminate undesired cells. This method allows...
Source: BioTechniques - August 8, 2023 Category: Biotechnology Authors: Prem Shrestha Stephen Rotarori Kirby Madden-Hennessey Christina Mohammed Chi-Han Yang Jordan Urbani Joseph Pettinelli Xueqi Liu Qi Zhao Source Type: research
