Quantitative analysis of lncRNA in formalin-fixed paraffin-embedded tissues of oral squamous cell carcinoma
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0033. Online ahead of print.ABSTRACTThe study evaluated expression profiles of few regulatory lncRNAs in oral squamous cell carcinoma and normal mucosa adjacent to oral cancer using paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues stored at a different duration of time (1-5 years) using real-time quantitative PCR. The quantity and quality of total RNA isolated from FFPE tissues was less compared with that of fresh frozen tissues, which resulted in a noncorrelation of quantification cycle values. Following normalization, the expression of lncRNAs in th...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Kiran Kumar Kaveri Hallikeri Ajaykumar Oli Mallikarjun Goni Apoorva Jain Jagadeesha Poyya Alagilavada S Shilpasree Palaksha Kanive Javaregowda Source Type: research
Releasing secrets bound to ancient remains with modern DNA extraction techniques: an interview with Elena Essel
Biotechniques. 2023 Aug;75(2):42-46. doi: 10.2144/btn-2023-0067. Epub 2023 Aug 17.ABSTRACTElena Essel (Msc) spoke to Ebony Torrington, Managing Editor of BioTechniques. Essel is a molecular biologist in Matthias Meyer's Advanced DNA Sequencing Techniques group at the Max Planck Institute for Evolutionary Anthropology in Leipzig (Germany). Essel studied biology at University of Erlangen-Nuremberg (Erlangen, Germany) for her bachelor's and in Martin-Luther-University Halle-Wittenberg (Halle an der Saale, Germany) for her master's. Essel worked in Meyer's group on DNA extraction of very degraded material for her master's thes...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Elena Essel Source Type: research
Peter and the Beanstalk: tackling the giant questions of soybean nodulation
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0060. Online ahead of print.ABSTRACTThe following is an edited interview, carried out by the Journal Development Editor of BioTechniques, Ashling Cannon, with Peter Gresshoff (University of Queensland, UQ; Brisbane, Australia). Peter is a plant developmental geneticist, using molecular and genetic tools to understand the complexities of gene networks during the control of nodule formation in legumes. He was the Director of the Center of Excellence for Integrative Legume Research and is now an Emeritus Professor at UQ.PMID:37589162 | DOI:10.2144/btn-2023-0060 (Source: BioTechniques)
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Peter Gresshoff Source Type: research
Quantitative analysis of lncRNA in formalin-fixed paraffin-embedded tissues of oral squamous cell carcinoma
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0033. Online ahead of print.ABSTRACTThe study evaluated expression profiles of few regulatory lncRNAs in oral squamous cell carcinoma and normal mucosa adjacent to oral cancer using paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues stored at a different duration of time (1-5 years) using real-time quantitative PCR. The quantity and quality of total RNA isolated from FFPE tissues was less compared with that of fresh frozen tissues, which resulted in a noncorrelation of quantification cycle values. Following normalization, the expression of lncRNAs in th...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Kiran Kumar Kaveri Hallikeri Ajaykumar Oli Mallikarjun Goni Apoorva Jain Jagadeesha Poyya Alagilavada S Shilpasree Palaksha Kanive Javaregowda Source Type: research
Releasing secrets bound to ancient remains with modern DNA extraction techniques: an interview with Elena Essel
Biotechniques. 2023 Aug;75(2):42-46. doi: 10.2144/btn-2023-0067. Epub 2023 Aug 17.ABSTRACTElena Essel (Msc) spoke to Ebony Torrington, Managing Editor of BioTechniques. Essel is a molecular biologist in Matthias Meyer's Advanced DNA Sequencing Techniques group at the Max Planck Institute for Evolutionary Anthropology in Leipzig (Germany). Essel studied biology at University of Erlangen-Nuremberg (Erlangen, Germany) for her bachelor's and in Martin-Luther-University Halle-Wittenberg (Halle an der Saale, Germany) for her master's. Essel worked in Meyer's group on DNA extraction of very degraded material for her master's thes...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Elena Essel Source Type: research
Peter and the Beanstalk: tackling the giant questions of soybean nodulation
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0060. Online ahead of print.ABSTRACTThe following is an edited interview, carried out by the Journal Development Editor of BioTechniques, Ashling Cannon, with Peter Gresshoff (University of Queensland, UQ; Brisbane, Australia). Peter is a plant developmental geneticist, using molecular and genetic tools to understand the complexities of gene networks during the control of nodule formation in legumes. He was the Director of the Center of Excellence for Integrative Legume Research and is now an Emeritus Professor at UQ.PMID:37589162 | DOI:10.2144/btn-2023-0060 (Source: BioTechniques)
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Peter Gresshoff Source Type: research
Quantitative analysis of lncRNA in formalin-fixed paraffin-embedded tissues of oral squamous cell carcinoma
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0033. Online ahead of print.ABSTRACTThe study evaluated expression profiles of few regulatory lncRNAs in oral squamous cell carcinoma and normal mucosa adjacent to oral cancer using paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues stored at a different duration of time (1-5 years) using real-time quantitative PCR. The quantity and quality of total RNA isolated from FFPE tissues was less compared with that of fresh frozen tissues, which resulted in a noncorrelation of quantification cycle values. Following normalization, the expression of lncRNAs in th...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Kiran Kumar Kaveri Hallikeri Ajaykumar Oli Mallikarjun Goni Apoorva Jain Jagadeesha Poyya Alagilavada S Shilpasree Palaksha Kanive Javaregowda Source Type: research
Releasing secrets bound to ancient remains with modern DNA extraction techniques: an interview with Elena Essel
Biotechniques. 2023 Aug;75(2):42-46. doi: 10.2144/btn-2023-0067. Epub 2023 Aug 17.ABSTRACTElena Essel (Msc) spoke to Ebony Torrington, Managing Editor of BioTechniques. Essel is a molecular biologist in Matthias Meyer's Advanced DNA Sequencing Techniques group at the Max Planck Institute for Evolutionary Anthropology in Leipzig (Germany). Essel studied biology at University of Erlangen-Nuremberg (Erlangen, Germany) for her bachelor's and in Martin-Luther-University Halle-Wittenberg (Halle an der Saale, Germany) for her master's. Essel worked in Meyer's group on DNA extraction of very degraded material for her master's thes...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Elena Essel Source Type: research
Peter and the Beanstalk: tackling the giant questions of soybean nodulation
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0060. Online ahead of print.ABSTRACTThe following is an edited interview, carried out by the Journal Development Editor of BioTechniques, Ashling Cannon, with Peter Gresshoff (University of Queensland, UQ; Brisbane, Australia). Peter is a plant developmental geneticist, using molecular and genetic tools to understand the complexities of gene networks during the control of nodule formation in legumes. He was the Director of the Center of Excellence for Integrative Legume Research and is now an Emeritus Professor at UQ.PMID:37589162 | DOI:10.2144/btn-2023-0060 (Source: BioTechniques)
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Peter Gresshoff Source Type: research
Quantitative analysis of lncRNA in formalin-fixed paraffin-embedded tissues of oral squamous cell carcinoma
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0033. Online ahead of print.ABSTRACTThe study evaluated expression profiles of few regulatory lncRNAs in oral squamous cell carcinoma and normal mucosa adjacent to oral cancer using paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues stored at a different duration of time (1-5 years) using real-time quantitative PCR. The quantity and quality of total RNA isolated from FFPE tissues was less compared with that of fresh frozen tissues, which resulted in a noncorrelation of quantification cycle values. Following normalization, the expression of lncRNAs in th...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Kiran Kumar Kaveri Hallikeri Ajaykumar Oli Mallikarjun Goni Apoorva Jain Jagadeesha Poyya Alagilavada S Shilpasree Palaksha Kanive Javaregowda Source Type: research
Releasing secrets bound to ancient remains with modern DNA extraction techniques: an interview with Elena Essel
Biotechniques. 2023 Aug;75(2):42-46. doi: 10.2144/btn-2023-0067. Epub 2023 Aug 17.ABSTRACTElena Essel (Msc) spoke to Ebony Torrington, Managing Editor of BioTechniques. Essel is a molecular biologist in Matthias Meyer's Advanced DNA Sequencing Techniques group at the Max Planck Institute for Evolutionary Anthropology in Leipzig (Germany). Essel studied biology at University of Erlangen-Nuremberg (Erlangen, Germany) for her bachelor's and in Martin-Luther-University Halle-Wittenberg (Halle an der Saale, Germany) for her master's. Essel worked in Meyer's group on DNA extraction of very degraded material for her master's thes...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Elena Essel Source Type: research
Peter and the Beanstalk: tackling the giant questions of soybean nodulation
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0060. Online ahead of print.ABSTRACTThe following is an edited interview, carried out by the Journal Development Editor of BioTechniques, Ashling Cannon, with Peter Gresshoff (University of Queensland, UQ; Brisbane, Australia). Peter is a plant developmental geneticist, using molecular and genetic tools to understand the complexities of gene networks during the control of nodule formation in legumes. He was the Director of the Center of Excellence for Integrative Legume Research and is now an Emeritus Professor at UQ.PMID:37589162 | DOI:10.2144/btn-2023-0060 (Source: BioTechniques)
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Peter Gresshoff Source Type: research
Quantitative analysis of lncRNA in formalin-fixed paraffin-embedded tissues of oral squamous cell carcinoma
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0033. Online ahead of print.ABSTRACTThe study evaluated expression profiles of few regulatory lncRNAs in oral squamous cell carcinoma and normal mucosa adjacent to oral cancer using paired fresh frozen and formalin-fixed paraffin-embedded (FFPE) tissues stored at a different duration of time (1-5 years) using real-time quantitative PCR. The quantity and quality of total RNA isolated from FFPE tissues was less compared with that of fresh frozen tissues, which resulted in a noncorrelation of quantification cycle values. Following normalization, the expression of lncRNAs in th...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Kiran Kumar Kaveri Hallikeri Ajaykumar Oli Mallikarjun Goni Apoorva Jain Jagadeesha Poyya Alagilavada S Shilpasree Palaksha Kanive Javaregowda Source Type: research
Releasing secrets bound to ancient remains with modern DNA extraction techniques: an interview with Elena Essel
Biotechniques. 2023 Aug;75(2):42-46. doi: 10.2144/btn-2023-0067. Epub 2023 Aug 17.ABSTRACTElena Essel (Msc) spoke to Ebony Torrington, Managing Editor of BioTechniques. Essel is a molecular biologist in Matthias Meyer's Advanced DNA Sequencing Techniques group at the Max Planck Institute for Evolutionary Anthropology in Leipzig (Germany). Essel studied biology at University of Erlangen-Nuremberg (Erlangen, Germany) for her bachelor's and in Martin-Luther-University Halle-Wittenberg (Halle an der Saale, Germany) for her master's. Essel worked in Meyer's group on DNA extraction of very degraded material for her master's thes...
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Elena Essel Source Type: research
Peter and the Beanstalk: tackling the giant questions of soybean nodulation
Biotechniques. 2023 Aug 17. doi: 10.2144/btn-2023-0060. Online ahead of print.ABSTRACTThe following is an edited interview, carried out by the Journal Development Editor of BioTechniques, Ashling Cannon, with Peter Gresshoff (University of Queensland, UQ; Brisbane, Australia). Peter is a plant developmental geneticist, using molecular and genetic tools to understand the complexities of gene networks during the control of nodule formation in legumes. He was the Director of the Center of Excellence for Integrative Legume Research and is now an Emeritus Professor at UQ.PMID:37589162 | DOI:10.2144/btn-2023-0060 (Source: BioTechniques)
Source: BioTechniques - August 17, 2023 Category: Biotechnology Authors: Peter Gresshoff Source Type: research
