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Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification
Biotechniques. 2024 Mar 28. doi: 10.2144/btn-2024-0012. Online ahead of print.ABSTRACTStrand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we report an enhanced approach by addition of single-stranded DNA binding protein, crowding agents and dUTP to enable amplification of kilobase-length products at low temperatures. Additionally, we pair this improved SDA with a novel carryover co...
Source: BioTechniques - March 28, 2024 Category: Biotechnology Authors: Yinhua Zhang Ashley N Luck Nathan A Tanner Source Type: research

Comparison and optimization of protocols and whole-genome capture conditions for ancient DNA samples
Biotechniques. 2024 Mar 26. doi: 10.2144/btn-2023-0107. Online ahead of print.ABSTRACTAncient DNA (aDNA) obtained from human remains is typically fragmented and present in relatively low amounts. Here we investigate a set of optimal methods for producing aDNA data by comparing silica-based DNA extraction and aDNA library preparation protocols. We also test the efficiency of whole-genome enrichment (WGC) on ancient human samples by modifying a number of parameter combinations. We find that the Dabney extraction protocol performs significantly better than alternatives. We further observed a positive trend with the BEST libra...
Source: BioTechniques - March 26, 2024 Category: Biotechnology Authors: Reyhan Yaka None Maja Krzewi ńska Vendela Kempe Lagerholm Anna Linderholm F üsun Özer Mehmet Somel Anders G ötherström Source Type: research

Recording the brain in vivo : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Apr;76(4):121-124. doi: 10.2144/btn-2024-0013. Epub 2024 Mar 14.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Recording the brain in vivo : emerging technologies for the exploration of mental health conditions
Biotechniques. 2024 Mar 14. doi: 10.2144/btn-2024-0013. Online ahead of print.ABSTRACTStandfirst Mounting interest in mental health conditions over the last two decades has been coupled with the increasing sophistication of techniques to study the brain in vivo. [Formula: see text].PMID:38482795 | DOI:10.2144/btn-2024-0013 (Source: BioTechniques)
Source: BioTechniques - March 14, 2024 Category: Biotechnology Authors: Tristan Free Source Type: research

Isolation and characterization of Paenibacillus peoriae JC-3jx from Dendrobium nobile 
In this study, endophytic bacteria from Dendrobium nobile were isolated using an improved method, showing inhibition of pathogens and growth promotion. JC-3jx, identified as Paenibacillus peoriae, exhibited significant inhibitory activity against tested fungi and bacteria, including Escherichia coli. JC-3jx also promoted corn seed rooting and Dendrobium growth, highlighting its excellent biocontrol and growth-promoting potential.PMID:38469872 | DOI:10.2144/btn-2023-0083 (Source: BioTechniques)
Source: BioTechniques - March 12, 2024 Category: Biotechnology Authors: ZhiPing Li Yuan Lin FeiFei Song RuoNan Zheng QinGeng Huang Source Type: research