Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Fully < em > in vitro < /em > iterative construction of a 24 kb-long artificial DNA sequence to store digital information
In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.PMID:38573592 | DOI:10.2144/btn-2023-0109 (Source: BioTechniques)
Source: BioTechniques - April 4, 2024 Category: Biotechnology Authors: Julien Leblanc Olivier Boulle Emeline Roux Jacques Nicolas Dominique Lavenier Yann Audic Source Type: research
Fully < em > in vitro < /em > iterative construction of a 24 kb-long artificial DNA sequence to store digital information
In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.PMID:38573592 | DOI:10.2144/btn-2023-0109 (Source: BioTechniques)
Source: BioTechniques - April 4, 2024 Category: Biotechnology Authors: Julien Leblanc Olivier Boulle Emeline Roux Jacques Nicolas Dominique Lavenier Yann Audic Source Type: research
Fully < em > in vitro < /em > iterative construction of a 24 kb-long artificial DNA sequence to store digital information
In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.PMID:38573592 | DOI:10.2144/btn-2023-0109 (Source: BioTechniques)
Source: BioTechniques - April 4, 2024 Category: Biotechnology Authors: Julien Leblanc Olivier Boulle Emeline Roux Jacques Nicolas Dominique Lavenier Yann Audic Source Type: research
Fully < em > in vitro < /em > iterative construction of a 24 kb-long artificial DNA sequence to store digital information
In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.PMID:38573592 | DOI:10.2144/btn-2023-0109 (Source: BioTechniques)
Source: BioTechniques - April 4, 2024 Category: Biotechnology Authors: Julien Leblanc Olivier Boulle Emeline Roux Jacques Nicolas Dominique Lavenier Yann Audic Source Type: research
Fully < em > in vitro < /em > iterative construction of a 24 kb-long artificial DNA sequence to store digital information
In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.PMID:38573592 | DOI:10.2144/btn-2023-0109 (Source: BioTechniques)
Source: BioTechniques - April 4, 2024 Category: Biotechnology Authors: Julien Leblanc Olivier Boulle Emeline Roux Jacques Nicolas Dominique Lavenier Yann Audic Source Type: research
Fully < em > in vitro < /em > iterative construction of a 24 kb-long artificial DNA sequence to store digital information
In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.PMID:38573592 | DOI:10.2144/btn-2023-0109 (Source: BioTechniques)
Source: BioTechniques - April 4, 2024 Category: Biotechnology Authors: Julien Leblanc Olivier Boulle Emeline Roux Jacques Nicolas Dominique Lavenier Yann Audic Source Type: research
Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification
Biotechniques. 2024 Mar 28. doi: 10.2144/btn-2024-0012. Online ahead of print.ABSTRACTStrand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we report an enhanced approach by addition of single-stranded DNA binding protein, crowding agents and dUTP to enable amplification of kilobase-length products at low temperatures. Additionally, we pair this improved SDA with a novel carryover co...
Source: BioTechniques - March 28, 2024 Category: Biotechnology Authors: Yinhua Zhang Ashley N Luck Nathan A Tanner Source Type: research
Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification
Biotechniques. 2024 Mar 28. doi: 10.2144/btn-2024-0012. Online ahead of print.ABSTRACTStrand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we report an enhanced approach by addition of single-stranded DNA binding protein, crowding agents and dUTP to enable amplification of kilobase-length products at low temperatures. Additionally, we pair this improved SDA with a novel carryover co...
Source: BioTechniques - March 28, 2024 Category: Biotechnology Authors: Yinhua Zhang Ashley N Luck Nathan A Tanner Source Type: research
Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification
Biotechniques. 2024 Mar 28. doi: 10.2144/btn-2024-0012. Online ahead of print.ABSTRACTStrand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we report an enhanced approach by addition of single-stranded DNA binding protein, crowding agents and dUTP to enable amplification of kilobase-length products at low temperatures. Additionally, we pair this improved SDA with a novel carryover co...
Source: BioTechniques - March 28, 2024 Category: Biotechnology Authors: Yinhua Zhang Ashley N Luck Nathan A Tanner Source Type: research
Isothermal amplification of long DNA fragments at low temperature by improved strand displacement amplification
Biotechniques. 2024 Mar 28. doi: 10.2144/btn-2024-0012. Online ahead of print.ABSTRACTStrand displacement amplification (SDA) is an isothermal amplification technique wherein amplification of a nucleic acid is initiated by nicking enzyme activity at sites flanking the target. Diagnostic SDA is very fast but requires precise optimization and is limited to very short amplicons. Here we report an enhanced approach by addition of single-stranded DNA binding protein, crowding agents and dUTP to enable amplification of kilobase-length products at low temperatures. Additionally, we pair this improved SDA with a novel carryover co...
Source: BioTechniques - March 28, 2024 Category: Biotechnology Authors: Yinhua Zhang Ashley N Luck Nathan A Tanner Source Type: research
