Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide
Conclusion: The H2S quantification method should be selected based on the specific requirements of a research project in terms of sensitivity, response time and cost-effectiveness.PMID:38059376 | DOI:10.2144/btn-2023-0075 (Source: BioTechniques)
Source: BioTechniques - December 7, 2023 Category: Biotechnology Authors: Susmit Mhatre Anjali Rai Hatim Ali Akash Patil Neetu Singh Richa Verma John Auden Cole Chandler Alekha Dash Catherine Opere Somnath Singh Source Type: research
Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide
Conclusion: The H2S quantification method should be selected based on the specific requirements of a research project in terms of sensitivity, response time and cost-effectiveness.PMID:38059376 | DOI:10.2144/btn-2023-0075 (Source: BioTechniques)
Source: BioTechniques - December 7, 2023 Category: Biotechnology Authors: Susmit Mhatre Anjali Rai Hatim Ali Akash Patil Neetu Singh Richa Verma John Auden Cole Chandler Alekha Dash Catherine Opere Somnath Singh Source Type: research
Comparison of colorimetric, spectroscopic and electrochemical techniques for quantification of hydrogen sulfide
Conclusion: The H2S quantification method should be selected based on the specific requirements of a research project in terms of sensitivity, response time and cost-effectiveness.PMID:38059376 | DOI:10.2144/btn-2023-0075 (Source: BioTechniques)
Source: BioTechniques - December 7, 2023 Category: Biotechnology Authors: Susmit Mhatre Anjali Rai Hatim Ali Akash Patil Neetu Singh Richa Verma John Auden Cole Chandler Alekha Dash Catherine Opere Somnath Singh Source Type: research
Cancer's sweet spot: techniques to harness saccharides in tumor biology
Biotechniques. 2023 Dec 4. doi: 10.2144/btn-2023-0110. Online ahead of print.ABSTRACTAll of the cells in our bodies are enveloped in sugar, this sweet coating plays a particularly interesting and crucial role in tumor biology. Here, we review the techniques being used to detect and exploit cancer's sweet spot. including click chemistry, glycoproteomic profiling and bioorthogonal chemistry.PMID:38047326 | DOI:10.2144/btn-2023-0110 (Source: BioTechniques)
Source: BioTechniques - December 4, 2023 Category: Biotechnology Authors: Jade Parker Source Type: research
Cancer's sweet spot: techniques to harness saccharides in tumor biology
Biotechniques. 2023 Dec 4. doi: 10.2144/btn-2023-0110. Online ahead of print.ABSTRACTAll of the cells in our bodies are enveloped in sugar, this sweet coating plays a particularly interesting and crucial role in tumor biology. Here, we review the techniques being used to detect and exploit cancer's sweet spot. including click chemistry, glycoproteomic profiling and bioorthogonal chemistry.PMID:38047326 | DOI:10.2144/btn-2023-0110 (Source: BioTechniques)
Source: BioTechniques - December 4, 2023 Category: Biotechnology Authors: Jade Parker Source Type: research
Cancer's sweet spot: techniques to harness saccharides in tumor biology
Biotechniques. 2023 Dec 4. doi: 10.2144/btn-2023-0110. Online ahead of print.ABSTRACTAll of the cells in our bodies are enveloped in sugar, this sweet coating plays a particularly interesting and crucial role in tumor biology. Here, we review the techniques being used to detect and exploit cancer's sweet spot. including click chemistry, glycoproteomic profiling and bioorthogonal chemistry.PMID:38047326 | DOI:10.2144/btn-2023-0110 (Source: BioTechniques)
Source: BioTechniques - December 4, 2023 Category: Biotechnology Authors: Jade Parker Source Type: research
Automated method for quantification of 20 amino acids in cell culture media during biopharmaceutical development
Biotechniques. 2023 Nov 24. doi: 10.2144/btn-2023-0068. Online ahead of print.ABSTRACTHerein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly present in cell culture media is described. The protocol facilitates detection of both primary and secondary amino acids through a two-step precolumn derivatization strategy using ortho-phthalaldehyde and 9-fluorenylmethyl chloroformate as derivatizing agents. The separation of derivatized amino acids with varying hydrophobicity is achieved through reverse-phase chromatography. The amino acids are simultaneously detected in a single workflow through the ...
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Ankita Dubey Srishti Joshi Kratika Upadhyay Ansuman Mahato Anurag S Rathore Source Type: research
PS4: a next-generation dataset for protein single-sequence secondary structure prediction
We present PS4, a dataset of 18,731 nonredundant protein chains and their respective secondary structure labels. Each chain is identified, and the dataset is nonredundant against other secondary structure datasets commonly seen in the literature. We perform ablation studies by training secondary structure prediction algorithms on the PS4 training set and obtains state-of-the-art accuracy on the CB513 test set in zero shots.PMID:37997848 | DOI:10.2144/btn-2023-0024 (Source: BioTechniques)
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Omar Peracha Source Type: research
Automated method for quantification of 20 amino acids in cell culture media during biopharmaceutical development
Biotechniques. 2023 Nov 24. doi: 10.2144/btn-2023-0068. Online ahead of print.ABSTRACTHerein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly present in cell culture media is described. The protocol facilitates detection of both primary and secondary amino acids through a two-step precolumn derivatization strategy using ortho-phthalaldehyde and 9-fluorenylmethyl chloroformate as derivatizing agents. The separation of derivatized amino acids with varying hydrophobicity is achieved through reverse-phase chromatography. The amino acids are simultaneously detected in a single workflow through the ...
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Ankita Dubey Srishti Joshi Kratika Upadhyay Ansuman Mahato Anurag S Rathore Source Type: research
PS4: a next-generation dataset for protein single-sequence secondary structure prediction
We present PS4, a dataset of 18,731 nonredundant protein chains and their respective secondary structure labels. Each chain is identified, and the dataset is nonredundant against other secondary structure datasets commonly seen in the literature. We perform ablation studies by training secondary structure prediction algorithms on the PS4 training set and obtains state-of-the-art accuracy on the CB513 test set in zero shots.PMID:37997848 | DOI:10.2144/btn-2023-0024 (Source: BioTechniques)
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Omar Peracha Source Type: research
Automated method for quantification of 20 amino acids in cell culture media during biopharmaceutical development
Biotechniques. 2023 Nov 24. doi: 10.2144/btn-2023-0068. Online ahead of print.ABSTRACTHerein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly present in cell culture media is described. The protocol facilitates detection of both primary and secondary amino acids through a two-step precolumn derivatization strategy using ortho-phthalaldehyde and 9-fluorenylmethyl chloroformate as derivatizing agents. The separation of derivatized amino acids with varying hydrophobicity is achieved through reverse-phase chromatography. The amino acids are simultaneously detected in a single workflow through the ...
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Ankita Dubey Srishti Joshi Kratika Upadhyay Ansuman Mahato Anurag S Rathore Source Type: research
PS4: a next-generation dataset for protein single-sequence secondary structure prediction
We present PS4, a dataset of 18,731 nonredundant protein chains and their respective secondary structure labels. Each chain is identified, and the dataset is nonredundant against other secondary structure datasets commonly seen in the literature. We perform ablation studies by training secondary structure prediction algorithms on the PS4 training set and obtains state-of-the-art accuracy on the CB513 test set in zero shots.PMID:37997848 | DOI:10.2144/btn-2023-0024 (Source: BioTechniques)
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Omar Peracha Source Type: research
Automated method for quantification of 20 amino acids in cell culture media during biopharmaceutical development
Biotechniques. 2023 Nov 24. doi: 10.2144/btn-2023-0068. Online ahead of print.ABSTRACTHerein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly present in cell culture media is described. The protocol facilitates detection of both primary and secondary amino acids through a two-step precolumn derivatization strategy using ortho-phthalaldehyde and 9-fluorenylmethyl chloroformate as derivatizing agents. The separation of derivatized amino acids with varying hydrophobicity is achieved through reverse-phase chromatography. The amino acids are simultaneously detected in a single workflow through the ...
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Ankita Dubey Srishti Joshi Kratika Upadhyay Ansuman Mahato Anurag S Rathore Source Type: research
PS4: a next-generation dataset for protein single-sequence secondary structure prediction
We present PS4, a dataset of 18,731 nonredundant protein chains and their respective secondary structure labels. Each chain is identified, and the dataset is nonredundant against other secondary structure datasets commonly seen in the literature. We perform ablation studies by training secondary structure prediction algorithms on the PS4 training set and obtains state-of-the-art accuracy on the CB513 test set in zero shots.PMID:37997848 | DOI:10.2144/btn-2023-0024 (Source: BioTechniques)
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Omar Peracha Source Type: research
Automated method for quantification of 20 amino acids in cell culture media during biopharmaceutical development
Biotechniques. 2023 Nov 24. doi: 10.2144/btn-2023-0068. Online ahead of print.ABSTRACTHerein, a step-by-step protocol for simultaneous detection of 20 amino acids commonly present in cell culture media is described. The protocol facilitates detection of both primary and secondary amino acids through a two-step precolumn derivatization strategy using ortho-phthalaldehyde and 9-fluorenylmethyl chloroformate as derivatizing agents. The separation of derivatized amino acids with varying hydrophobicity is achieved through reverse-phase chromatography. The amino acids are simultaneously detected in a single workflow through the ...
Source: BioTechniques - November 24, 2023 Category: Biotechnology Authors: Ankita Dubey Srishti Joshi Kratika Upadhyay Ansuman Mahato Anurag S Rathore Source Type: research
