An industry perspective approach and control strategy for implementation of ready-to-use cells in bioassays: survey outcome and recommendations
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0086. Online ahead of print.ABSTRACTThe BioPhorum Development Group is an industry collaboration enabling the sharing of common practices for the development of biopharmaceuticals. Bioassays are an important part of an analytical control system. Utilization of ready-to-use cells can increase operational flexibility and improve efficiency by providing frozen cell banks uniform stock while removing challenges associated with maintaining cultured cells. The BioPhorum Development Group-Bioassay workstream conducted an intercompany benchmarking survey and group discussions around...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: John R White Sammina Ahmed Jan Amstrup Petra Bennington Zhijie Jey Cheng Peter Day Keneshia K Haenssen Adi Kozminsky-Atias Eva Martinez Esther Hq Ong Isam Qahwash Elaine Se Stokes Jenny Wang Source Type: research
Extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0092. Online ahead of print.ABSTRACTModern approaches to discovering molecular mechanisms and validating treatments for age-related neuromusculoskeletal dysfunction typically rely on high-throughput transcriptome analysis. Previously harvested and fixed tissues offer an incredible reservoir of untapped molecular information. However, obtaining RNA from such formaldehyde-fixed neuromusculoskeletal tissues, especially fibrotic aged tissues, is technically challenging and often results in RNA degradation, chemical modification and yield reduction, prohibiting further analysis. ...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: Seth D Thompson Rajeswari Pichika Richard L Lieber Mitra Lavasani Source Type: research
An industry perspective approach and control strategy for implementation of ready-to-use cells in bioassays: survey outcome and recommendations
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0086. Online ahead of print.ABSTRACTThe BioPhorum Development Group is an industry collaboration enabling the sharing of common practices for the development of biopharmaceuticals. Bioassays are an important part of an analytical control system. Utilization of ready-to-use cells can increase operational flexibility and improve efficiency by providing frozen cell banks uniform stock while removing challenges associated with maintaining cultured cells. The BioPhorum Development Group-Bioassay workstream conducted an intercompany benchmarking survey and group discussions around...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: John R White Sammina Ahmed Jan Amstrup Petra Bennington Zhijie Jey Cheng Peter Day Keneshia K Haenssen Adi Kozminsky-Atias Eva Martinez Esther Hq Ong Isam Qahwash Elaine Se Stokes Jenny Wang Source Type: research
Extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0092. Online ahead of print.ABSTRACTModern approaches to discovering molecular mechanisms and validating treatments for age-related neuromusculoskeletal dysfunction typically rely on high-throughput transcriptome analysis. Previously harvested and fixed tissues offer an incredible reservoir of untapped molecular information. However, obtaining RNA from such formaldehyde-fixed neuromusculoskeletal tissues, especially fibrotic aged tissues, is technically challenging and often results in RNA degradation, chemical modification and yield reduction, prohibiting further analysis. ...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: Seth D Thompson Rajeswari Pichika Richard L Lieber Mitra Lavasani Source Type: research
An industry perspective approach and control strategy for implementation of ready-to-use cells in bioassays: survey outcome and recommendations
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0086. Online ahead of print.ABSTRACTThe BioPhorum Development Group is an industry collaboration enabling the sharing of common practices for the development of biopharmaceuticals. Bioassays are an important part of an analytical control system. Utilization of ready-to-use cells can increase operational flexibility and improve efficiency by providing frozen cell banks uniform stock while removing challenges associated with maintaining cultured cells. The BioPhorum Development Group-Bioassay workstream conducted an intercompany benchmarking survey and group discussions around...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: John R White Sammina Ahmed Jan Amstrup Petra Bennington Zhijie Jey Cheng Peter Day Keneshia K Haenssen Adi Kozminsky-Atias Eva Martinez Esther Hq Ong Isam Qahwash Elaine Se Stokes Jenny Wang Source Type: research
Extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0092. Online ahead of print.ABSTRACTModern approaches to discovering molecular mechanisms and validating treatments for age-related neuromusculoskeletal dysfunction typically rely on high-throughput transcriptome analysis. Previously harvested and fixed tissues offer an incredible reservoir of untapped molecular information. However, obtaining RNA from such formaldehyde-fixed neuromusculoskeletal tissues, especially fibrotic aged tissues, is technically challenging and often results in RNA degradation, chemical modification and yield reduction, prohibiting further analysis. ...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: Seth D Thompson Rajeswari Pichika Richard L Lieber Mitra Lavasani Source Type: research
An industry perspective approach and control strategy for implementation of ready-to-use cells in bioassays: survey outcome and recommendations
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0086. Online ahead of print.ABSTRACTThe BioPhorum Development Group is an industry collaboration enabling the sharing of common practices for the development of biopharmaceuticals. Bioassays are an important part of an analytical control system. Utilization of ready-to-use cells can increase operational flexibility and improve efficiency by providing frozen cell banks uniform stock while removing challenges associated with maintaining cultured cells. The BioPhorum Development Group-Bioassay workstream conducted an intercompany benchmarking survey and group discussions around...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: John R White Sammina Ahmed Jan Amstrup Petra Bennington Zhijie Jey Cheng Peter Day Keneshia K Haenssen Adi Kozminsky-Atias Eva Martinez Esther Hq Ong Isam Qahwash Elaine Se Stokes Jenny Wang Source Type: research
Extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0092. Online ahead of print.ABSTRACTModern approaches to discovering molecular mechanisms and validating treatments for age-related neuromusculoskeletal dysfunction typically rely on high-throughput transcriptome analysis. Previously harvested and fixed tissues offer an incredible reservoir of untapped molecular information. However, obtaining RNA from such formaldehyde-fixed neuromusculoskeletal tissues, especially fibrotic aged tissues, is technically challenging and often results in RNA degradation, chemical modification and yield reduction, prohibiting further analysis. ...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: Seth D Thompson Rajeswari Pichika Richard L Lieber Mitra Lavasani Source Type: research
An industry perspective approach and control strategy for implementation of ready-to-use cells in bioassays: survey outcome and recommendations
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0086. Online ahead of print.ABSTRACTThe BioPhorum Development Group is an industry collaboration enabling the sharing of common practices for the development of biopharmaceuticals. Bioassays are an important part of an analytical control system. Utilization of ready-to-use cells can increase operational flexibility and improve efficiency by providing frozen cell banks uniform stock while removing challenges associated with maintaining cultured cells. The BioPhorum Development Group-Bioassay workstream conducted an intercompany benchmarking survey and group discussions around...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: John R White Sammina Ahmed Jan Amstrup Petra Bennington Zhijie Jey Cheng Peter Day Keneshia K Haenssen Adi Kozminsky-Atias Eva Martinez Esther Hq Ong Isam Qahwash Elaine Se Stokes Jenny Wang Source Type: research
Extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0092. Online ahead of print.ABSTRACTModern approaches to discovering molecular mechanisms and validating treatments for age-related neuromusculoskeletal dysfunction typically rely on high-throughput transcriptome analysis. Previously harvested and fixed tissues offer an incredible reservoir of untapped molecular information. However, obtaining RNA from such formaldehyde-fixed neuromusculoskeletal tissues, especially fibrotic aged tissues, is technically challenging and often results in RNA degradation, chemical modification and yield reduction, prohibiting further analysis. ...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: Seth D Thompson Rajeswari Pichika Richard L Lieber Mitra Lavasani Source Type: research
An industry perspective approach and control strategy for implementation of ready-to-use cells in bioassays: survey outcome and recommendations
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0086. Online ahead of print.ABSTRACTThe BioPhorum Development Group is an industry collaboration enabling the sharing of common practices for the development of biopharmaceuticals. Bioassays are an important part of an analytical control system. Utilization of ready-to-use cells can increase operational flexibility and improve efficiency by providing frozen cell banks uniform stock while removing challenges associated with maintaining cultured cells. The BioPhorum Development Group-Bioassay workstream conducted an intercompany benchmarking survey and group discussions around...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: John R White Sammina Ahmed Jan Amstrup Petra Bennington Zhijie Jey Cheng Peter Day Keneshia K Haenssen Adi Kozminsky-Atias Eva Martinez Esther Hq Ong Isam Qahwash Elaine Se Stokes Jenny Wang Source Type: research
Extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues
Biotechniques. 2024 Feb 9. doi: 10.2144/btn-2023-0092. Online ahead of print.ABSTRACTModern approaches to discovering molecular mechanisms and validating treatments for age-related neuromusculoskeletal dysfunction typically rely on high-throughput transcriptome analysis. Previously harvested and fixed tissues offer an incredible reservoir of untapped molecular information. However, obtaining RNA from such formaldehyde-fixed neuromusculoskeletal tissues, especially fibrotic aged tissues, is technically challenging and often results in RNA degradation, chemical modification and yield reduction, prohibiting further analysis. ...
Source: BioTechniques - February 9, 2024 Category: Biotechnology Authors: Seth D Thompson Rajeswari Pichika Richard L Lieber Mitra Lavasani Source Type: research
A novel method for sampling subgingival microbiome: a comparative metatranscriptomic study
Biotechniques. 2024 Feb 6. doi: 10.2144/btn-2023-0076. Online ahead of print.ABSTRACTThe subgingival microbiome has been implicated in oral and systemic diseases such as periodontitis and Alzheimer's disease. However, subgingival sampling is challenging. We developed a novel method of sampling the subgingival microbiome by rotationally swabbing the supragingival area, named subgingival-P (for proxy) samples. We sampled and metatranscriptomically analyzed subgingival and subgingival-P samples of three different teeth in 20 individuals. The subgingival-P samples were comparable to the subgingival samples in the relative abun...
Source: BioTechniques - February 6, 2024 Category: Biotechnology Authors: Diana Demusaj Ryan Toma Tanveer Khan Lan Hu Guruduth Banavar Momchilo Vuyisich Source Type: research
High-throughput screening for a SARS-CoV-2 frameshifting inhibitor using a cell-free protein synthesis system
Biotechniques. 2024 Jan 31. doi: 10.2144/btn-2023-0102. Online ahead of print.ABSTRACTProgrammed-1 ribosomal frameshifting (-1 PRF) is a translational mechanism adopted by some viruses, including SARS-CoV-2. To find a compound that can inhibit -1 PRF in SARS-CoV-2, we set up a high-throughput screening system using a HeLa cell extract-derived cell-free protein synthesis (CFPS) system. A total of 32,000 compounds were individually incubated with the CFPS system programmed with a -1 PRF-EGFP template. Several compounds were observed to decrease the -1 PRF-driven fluorescence, and one of them had some suppressive effect on -1...
Source: BioTechniques - January 31, 2024 Category: Biotechnology Authors: Kodai Machida Rin Tanaka Seraya Miki Shotaro Noseda Mayumi Yuasa-Sunagawa Hiroaki Imataka Source Type: research
Welcome to the 76th Issue of < em > BioTechniques < /em >
Biotechniques. 2024 Jan;76(1):1-4. doi: 10.2144/btn-2023-0113.NO ABSTRACTPMID:38213038 | DOI:10.2144/btn-2023-0113 (Source: BioTechniques)
Source: BioTechniques - January 12, 2024 Category: Biotechnology Authors: Ashling Cannon Ebony Torrington Tristan Free Source Type: research
