Fully < em > in vitro < /em > iterative construction of a 24  kb-long artificial DNA sequence to store digital information

In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.PMID:38573592 | DOI:10.2144/btn-2023-0109
Source: BioTechniques - Category: Biotechnology Authors: Source Type: research