The Syntheses of Chromium Aluminum Carbide (Cr < sub > 2 < /sub > AlC) MAX  Phase and Cr < sub > 2 < /sub > CTx MXene and Investigation of Their Antimicrobial Properties
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04910-w. Online ahead of print.ABSTRACTChromium aluminum carbide (Cr2AlC) MAX phase and Cr2CTx (MXene-Cr) were synthesized by the pressureless sintering method and hydrothermal method, respectively. In addition to this, the free radical scavenging activities (FRSA) of MAX-Cr phase and MXene-Cr compounds were tested and compared with ascorbic acid and trolox as standard compounds. The obtained FRSA results of MAX-Cr phase and MXene-Cr were 42.82 and 59.64%, respectively, at 100 mg/L concentration. MXene-Cr showed a 66.90% inhibitory effect on α-amylase at 200 mg...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Gul Kaya Eda Ondul Koc Sadin Özdemir M Serkan Yal çın Kasim Ocakoglu Nadir Dizge Source Type: research

Exploring the Mechanism of Acupuncture's Influence on the Protease Activity and Free Radical Damage in Synovial Fluid of Rheumatoid Arthritis Rats Induced by Type II Collagen from the Release of Active Oxygen
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04932-4. Online ahead of print.ABSTRACTAcupuncture was studied to investigate the mechanism of its effect on protease vitality and free radical damage in Type I CIA rats induced by type II collagen. The study divided rats into a control group (injected with physiological saline, n = 10), a model group (injected with type II collagen, n = 10), and an intervention group (injected with type II collagen + acupuncture ST36 and GB39, 3 times a week, for a total of 4 weeks, n = 10) based on the different injected drugs. Then, various indicators of the mice were experim...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Kaiying Cui Dezhong Bi Fuyan Wang Pengfei Hou Jinxing Guo Zhanwang Xu Source Type: research

Improving the Catalytic Properties of Xylanase from Alteromones Macleadii H35 Through Sequence Analysis
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04936-0. Online ahead of print.ABSTRACTEndo-1,4-β-xylanase is a key xylanolytic enzyme, and our study aimed to enhance the catalytic properties of Alteromones Macleadii xylanase (Xyn ZT-2) through sequence-guided design approach. Analysis of the amino acid sequence revealed highly conserved residues near the active site, with few differences. Introducing various mutations allowed us to modify the enzyme's catalytic performance. Particularly, the A152G mutation led to a 9.8-fold increase in activity and a 23.2-fold increase in catalytic efficiency. Moreover, A15...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Caixia Cui Jia Xu Juntao Wu Ningning Wang Zhao Zhang Chenyan Zhou Source Type: research

Correlation of Differentially Expressed lncRNAs with Intestinal Flora Imbalance, Small Intestinal Permeability, and Glucose Uptake in T2DM Mice
This study aimed to investigate the correlation between differentially expressed lncRNAs in mice with type 2 diabetes mellitus (T2DM) and alterations in the intestinal flora and intestinal pathology. A T2DM mouse model was constructed by feeding mice a high-fat diet. Serum fat metabolism-related indices and insulin levels were biochemically detected. Serum inflammatory factors (IL-1β, IL-6, TNF-α, IL-10) and endotoxin (LPS) were measured by ELISA. Histopathological changes in the small intestines of mice were observed by HE. The short-chain fatty acid (SCFA) content was analyzed using GC-MS. Analysis of altered intestina...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Shufang Xu Heng Zhang Hui Zou Zhitao Chen Ting Jiang Mengjun Huang Source Type: research

The Syntheses of Chromium Aluminum Carbide (Cr < sub > 2 < /sub > AlC) MAX  Phase and Cr < sub > 2 < /sub > CTx MXene and Investigation of Their Antimicrobial Properties
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04910-w. Online ahead of print.ABSTRACTChromium aluminum carbide (Cr2AlC) MAX phase and Cr2CTx (MXene-Cr) were synthesized by the pressureless sintering method and hydrothermal method, respectively. In addition to this, the free radical scavenging activities (FRSA) of MAX-Cr phase and MXene-Cr compounds were tested and compared with ascorbic acid and trolox as standard compounds. The obtained FRSA results of MAX-Cr phase and MXene-Cr were 42.82 and 59.64%, respectively, at 100 mg/L concentration. MXene-Cr showed a 66.90% inhibitory effect on α-amylase at 200 mg...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Gul Kaya Eda Ondul Koc Sadin Özdemir M Serkan Yal çın Kasim Ocakoglu Nadir Dizge Source Type: research

Exploring the Mechanism of Acupuncture's Influence on the Protease Activity and Free Radical Damage in Synovial Fluid of Rheumatoid Arthritis Rats Induced by Type II Collagen from the Release of Active Oxygen
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04932-4. Online ahead of print.ABSTRACTAcupuncture was studied to investigate the mechanism of its effect on protease vitality and free radical damage in Type I CIA rats induced by type II collagen. The study divided rats into a control group (injected with physiological saline, n = 10), a model group (injected with type II collagen, n = 10), and an intervention group (injected with type II collagen + acupuncture ST36 and GB39, 3 times a week, for a total of 4 weeks, n = 10) based on the different injected drugs. Then, various indicators of the mice were experim...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Kaiying Cui Dezhong Bi Fuyan Wang Pengfei Hou Jinxing Guo Zhanwang Xu Source Type: research

Improving the Catalytic Properties of Xylanase from Alteromones Macleadii H35 Through Sequence Analysis
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04936-0. Online ahead of print.ABSTRACTEndo-1,4-β-xylanase is a key xylanolytic enzyme, and our study aimed to enhance the catalytic properties of Alteromones Macleadii xylanase (Xyn ZT-2) through sequence-guided design approach. Analysis of the amino acid sequence revealed highly conserved residues near the active site, with few differences. Introducing various mutations allowed us to modify the enzyme's catalytic performance. Particularly, the A152G mutation led to a 9.8-fold increase in activity and a 23.2-fold increase in catalytic efficiency. Moreover, A15...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Caixia Cui Jia Xu Juntao Wu Ningning Wang Zhao Zhang Chenyan Zhou Source Type: research

Correlation of Differentially Expressed lncRNAs with Intestinal Flora Imbalance, Small Intestinal Permeability, and Glucose Uptake in T2DM Mice
This study aimed to investigate the correlation between differentially expressed lncRNAs in mice with type 2 diabetes mellitus (T2DM) and alterations in the intestinal flora and intestinal pathology. A T2DM mouse model was constructed by feeding mice a high-fat diet. Serum fat metabolism-related indices and insulin levels were biochemically detected. Serum inflammatory factors (IL-1β, IL-6, TNF-α, IL-10) and endotoxin (LPS) were measured by ELISA. Histopathological changes in the small intestines of mice were observed by HE. The short-chain fatty acid (SCFA) content was analyzed using GC-MS. Analysis of altered intestina...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Shufang Xu Heng Zhang Hui Zou Zhitao Chen Ting Jiang Mengjun Huang Source Type: research

The Syntheses of Chromium Aluminum Carbide (Cr < sub > 2 < /sub > AlC) MAX  Phase and Cr < sub > 2 < /sub > CTx MXene and Investigation of Their Antimicrobial Properties
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04910-w. Online ahead of print.ABSTRACTChromium aluminum carbide (Cr2AlC) MAX phase and Cr2CTx (MXene-Cr) were synthesized by the pressureless sintering method and hydrothermal method, respectively. In addition to this, the free radical scavenging activities (FRSA) of MAX-Cr phase and MXene-Cr compounds were tested and compared with ascorbic acid and trolox as standard compounds. The obtained FRSA results of MAX-Cr phase and MXene-Cr were 42.82 and 59.64%, respectively, at 100 mg/L concentration. MXene-Cr showed a 66.90% inhibitory effect on α-amylase at 200 mg...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Gul Kaya Eda Ondul Koc Sadin Özdemir M Serkan Yal çın Kasim Ocakoglu Nadir Dizge Source Type: research

Exploring the Mechanism of Acupuncture's Influence on the Protease Activity and Free Radical Damage in Synovial Fluid of Rheumatoid Arthritis Rats Induced by Type II Collagen from the Release of Active Oxygen
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04932-4. Online ahead of print.ABSTRACTAcupuncture was studied to investigate the mechanism of its effect on protease vitality and free radical damage in Type I CIA rats induced by type II collagen. The study divided rats into a control group (injected with physiological saline, n = 10), a model group (injected with type II collagen, n = 10), and an intervention group (injected with type II collagen + acupuncture ST36 and GB39, 3 times a week, for a total of 4 weeks, n = 10) based on the different injected drugs. Then, various indicators of the mice were experim...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Kaiying Cui Dezhong Bi Fuyan Wang Pengfei Hou Jinxing Guo Zhanwang Xu Source Type: research

Improving the Catalytic Properties of Xylanase from Alteromones Macleadii H35 Through Sequence Analysis
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04936-0. Online ahead of print.ABSTRACTEndo-1,4-β-xylanase is a key xylanolytic enzyme, and our study aimed to enhance the catalytic properties of Alteromones Macleadii xylanase (Xyn ZT-2) through sequence-guided design approach. Analysis of the amino acid sequence revealed highly conserved residues near the active site, with few differences. Introducing various mutations allowed us to modify the enzyme's catalytic performance. Particularly, the A152G mutation led to a 9.8-fold increase in activity and a 23.2-fold increase in catalytic efficiency. Moreover, A15...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Caixia Cui Jia Xu Juntao Wu Ningning Wang Zhao Zhang Chenyan Zhou Source Type: research

Correlation of Differentially Expressed lncRNAs with Intestinal Flora Imbalance, Small Intestinal Permeability, and Glucose Uptake in T2DM Mice
This study aimed to investigate the correlation between differentially expressed lncRNAs in mice with type 2 diabetes mellitus (T2DM) and alterations in the intestinal flora and intestinal pathology. A T2DM mouse model was constructed by feeding mice a high-fat diet. Serum fat metabolism-related indices and insulin levels were biochemically detected. Serum inflammatory factors (IL-1β, IL-6, TNF-α, IL-10) and endotoxin (LPS) were measured by ELISA. Histopathological changes in the small intestines of mice were observed by HE. The short-chain fatty acid (SCFA) content was analyzed using GC-MS. Analysis of altered intestina...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Shufang Xu Heng Zhang Hui Zou Zhitao Chen Ting Jiang Mengjun Huang Source Type: research

The Syntheses of Chromium Aluminum Carbide (Cr < sub > 2 < /sub > AlC) MAX  Phase and Cr < sub > 2 < /sub > CTx MXene and Investigation of Their Antimicrobial Properties
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04910-w. Online ahead of print.ABSTRACTChromium aluminum carbide (Cr2AlC) MAX phase and Cr2CTx (MXene-Cr) were synthesized by the pressureless sintering method and hydrothermal method, respectively. In addition to this, the free radical scavenging activities (FRSA) of MAX-Cr phase and MXene-Cr compounds were tested and compared with ascorbic acid and trolox as standard compounds. The obtained FRSA results of MAX-Cr phase and MXene-Cr were 42.82 and 59.64%, respectively, at 100 mg/L concentration. MXene-Cr showed a 66.90% inhibitory effect on α-amylase at 200 mg...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Gul Kaya Eda Ondul Koc Sadin Özdemir M Serkan Yal çın Kasim Ocakoglu Nadir Dizge Source Type: research

Exploring the Mechanism of Acupuncture's Influence on the Protease Activity and Free Radical Damage in Synovial Fluid of Rheumatoid Arthritis Rats Induced by Type II Collagen from the Release of Active Oxygen
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04932-4. Online ahead of print.ABSTRACTAcupuncture was studied to investigate the mechanism of its effect on protease vitality and free radical damage in Type I CIA rats induced by type II collagen. The study divided rats into a control group (injected with physiological saline, n = 10), a model group (injected with type II collagen, n = 10), and an intervention group (injected with type II collagen + acupuncture ST36 and GB39, 3 times a week, for a total of 4 weeks, n = 10) based on the different injected drugs. Then, various indicators of the mice were experim...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Kaiying Cui Dezhong Bi Fuyan Wang Pengfei Hou Jinxing Guo Zhanwang Xu Source Type: research

Improving the Catalytic Properties of Xylanase from Alteromones Macleadii H35 Through Sequence Analysis
Appl Biochem Biotechnol. 2024 Mar 28. doi: 10.1007/s12010-024-04936-0. Online ahead of print.ABSTRACTEndo-1,4-β-xylanase is a key xylanolytic enzyme, and our study aimed to enhance the catalytic properties of Alteromones Macleadii xylanase (Xyn ZT-2) through sequence-guided design approach. Analysis of the amino acid sequence revealed highly conserved residues near the active site, with few differences. Introducing various mutations allowed us to modify the enzyme's catalytic performance. Particularly, the A152G mutation led to a 9.8-fold increase in activity and a 23.2-fold increase in catalytic efficiency. Moreover, A15...
Source: Applied Biochemistry and Biotechnology - March 28, 2024 Category: Biochemistry Authors: Caixia Cui Jia Xu Juntao Wu Ningning Wang Zhao Zhang Chenyan Zhou Source Type: research