Applied Biochemistry and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Preparation of Yeast Extract from Brewer's Yeast Waste and Its Potential Application as a Medium Constituent
This study aims to utilize the autolysis of discarded yeast post beer brewing to produce yeast extract. The concentration, temperature, pH, and time conditions are systematically optimized. It reveals that the yield of amino nitrogen and solids in the extract was increased by 3.3% and 20.9% under the optimized conditions (1.2% wall-breaking enzyme, 1% yeast extract enzyme, and a hydrolysis time of 24 h) than that of the documented 4.03% and 69.05%. Additionally, a comparative analysis with commercially available yeast powder demonstrates that the yeast extract derived from this study adequately fulfills the nutritional req...
Source: Applied Biochemistry and Biotechnology - February 22, 2024 Category: Biochemistry Authors: Feng Gao Qingfei Li Wei Wei Yutao Wang Wancheng Song Xurui Yang Huai Ji Jia Zhou Ya Xin Zhongbiao Tan Jianjun Pei Hao Shi Source Type: research

A New Method for Selective Extraction of Torularhodin from Red Yeast Using CO < sub > 2 < /sub > -SFE Technique
Appl Biochem Biotechnol. 2024 Feb 22. doi: 10.1007/s12010-024-04884-9. Online ahead of print.ABSTRACTTorularhodin is a dark pink colored carotenoid belonging to the xanthophylls group that can be biologically synthesized by red yeasts, especially by Rhodotorula and Sporobolomyces genera. The growing interest in this molecule is due to its biological activities such as antioxidant, anticholesterolemic, anti-inflammatory, antimicrobial, and anticancer. To satisfy potential commercial markets, numerous methods have been proposed to develop a cost-effective and environmentally friendly downstream process for the purification o...
Source: Applied Biochemistry and Biotechnology - February 22, 2024 Category: Biochemistry Authors: Alfredo Ambrico Vincenzo Larocca Mario Trupo Maria Martino Rosaria Alessandra Magarelli Anna Spagnoletta Roberto Balducchi Source Type: research

Efficient Expression and Characterization of an Endo-Type Lyase HCLase_M28 and Its Gradual Scale-Up Fermentation for the Preparation of Chondroitin Sulfate Oligosaccharides
Appl Biochem Biotechnol. 2024 Feb 22. doi: 10.1007/s12010-024-04878-7. Online ahead of print.ABSTRACTGlycosaminoglycan (GAG) lyases have been critical in structural and functional studies of GAGs. HCLase_M28, a lyase identified from the genome of Microbacterium sp. M28 was heterologously expressed, enzymatically characterized, and prepared in large-scale fermentation for the production of chondroitin sulfate (CS) oligosaccharides. Results showed that the expression of HCLase_M28 in Escherichia coli BL21 (DE3)-pET24a-HCLase_M28opt1 and Bacillus subtilis W800-pSTOP1622-HCLase_M28opt2 were 108-fold and 25-fold that of wide st...
Source: Applied Biochemistry and Biotechnology - February 22, 2024 Category: Biochemistry Authors: Ruibao Ju Baoqin Han Feng Han Yanfei Peng Source Type: research

Characterization and Application of an Aspartate Dehydrogenase from Achromobacter denitrificans
Appl Biochem Biotechnol. 2024 Feb 22. doi: 10.1007/s12010-024-04867-w. Online ahead of print.ABSTRACTA novel gene encoding aspartate dehydrogenase (ASPDH) has been discovered in Achromobacter denitrificans. The product of this gene has a strict dependence on NADH and demonstrated significant reductive activity towards not only oxaloacetate (OAA) but also 2-ketobutyric acid. Further enzymatic characterization revealed the kinetic parameters of ASPDH for OAA and 2-ketobutyric acid were as follows: Km values of 4.25 mM and 0.89 mM, Vmax values of 10.67 U mg-1 and 2.10 U mg-1, and Kcat values of 3.70 s-1 and 0.72 s-1, respecti...
Source: Applied Biochemistry and Biotechnology - February 22, 2024 Category: Biochemistry Authors: Zifeng Wang Wenjing Liu Yi Yan Tai-Ping Fan Yujie Cai Source Type: research

Heterologous Expression and Characterization of a Novel Mesophilic Maltogenic α-Amylase AmyFlA from Flavobacterium sp. NAU1659
Appl Biochem Biotechnol. 2024 Feb 22. doi: 10.1007/s12010-024-04874-x. Online ahead of print.ABSTRACTA novel amylase AmyFlA from Flavobacterium sp. NAU1659, AmyFlA, was cloned and expressed in Esherichia coli. Based on phylogenetic and functional analysis, it was identified as a novel member of the subfamily GH13_46, sharing high sequence identity. The protein was predicted to consist of 620 amino acids, with a putative signal peptide of 25 amino acids. The enzyme was able to hydrolyze soluble starch with a specific activity of 352.97 U/mg at 50 °C in 50 mM phosphate buffer (pH 6.0). The Km and Vmax values of AmyFlA were ...
Source: Applied Biochemistry and Biotechnology - February 22, 2024 Category: Biochemistry Authors: Yanxin Wang Tingting Xie Guanhua Yan Huairen Xue Zhensong Zhao Xianfeng Ye Source Type: research

Optimal Enzyme-Assisted Extraction of Phenolics from Leaves of Pongamia pinnata via Response Surface Methodology and Artificial Neural Networking
Appl Biochem Biotechnol. 2024 Feb 22. doi: 10.1007/s12010-024-04875-w. Online ahead of print.ABSTRACTThis research work seeks to evaluate the impact of selected enzyme complexes on the optimised release of phenolics from leaves of Pongamia pinnata. After preliminary solvent extraction, the P. pinnata leaf extract was subjected to enzymatic treatment, using enzyme cocktails such as kemzyme dry-plus, natuzyme, and zympex-014. It was noticed that zympex-014 had a greater extract yield (28.0%) than kemzyme dry-plus (17.0%) and natuzyme (18.0%). Based on the better outcomes, zympex-014-based extract values were subsequently app...
Source: Applied Biochemistry and Biotechnology - February 22, 2024 Category: Biochemistry Authors: Tariq Aziz Rahman Qadir Farooq Anwar Sumaira Naz Nausheen Nazir Ghulam Nabi Cui Haiying Lin Lin Metab Alharbi Abdullah F Alasmari Source Type: research

Facilely Prepared Carbon Dots as Effective Anode Modifier for Enhanced Performance of Microbial Fuel Cells
Appl Biochem Biotechnol. 2024 Feb 22. doi: 10.1007/s12010-024-04864-z. Online ahead of print.ABSTRACTMicrobial fuel cells (MFCs) are a promising technology for obtaining energy in wastewater. Effective extracellular electron transfer is one of the key factors for its practical application. In this work, carbon dots (CDs) enriched with oxygen-containing groups on the surface were synthesized as an efficient anode modifier using a simple hydrothermal method and common reactants. The experimental findings indicated that anodes modified with CDs exhibited increased electrical conductivity and greater hydrophilicity. These modi...
Source: Applied Biochemistry and Biotechnology - February 22, 2024 Category: Biochemistry Authors: Hui-Xu Wei Rui Qiu Ai-Yi Li Liu-Jie Liang Yan-Nan Feng Shu-Hua Li Nan Li Source Type: research

Integrating Network Pharmacology and Experimental Verification to Explore the Pharmacological Mechanisms of Radix Paeoniae Rubra Against Glioma
Appl Biochem Biotechnol. 2024 Feb 21. doi: 10.1007/s12010-024-04887-6. Online ahead of print.ABSTRACTGlioma has a high mortality and can hardly be completely cured. Radix Paeoniae Rubra (RPR) is a prevalent component in traditional Chinese medicine used for tumor treatments. We explored the mechanism of RPR in treating glioma using network pharmacology and experiments. A network pharmacology approach was used to screen active ingredients, targets of RPR and glioma. We then constructed a herb-active ingredient-target-pathway network and conducted protein-protein interaction (PPI) network analysis, as well as Gene Ontology (...
Source: Applied Biochemistry and Biotechnology - February 21, 2024 Category: Biochemistry Authors: Siyu Zhu Qianxu Jin Shiyang Zhang Zihan Song Shiqi Zhang Zongmao Zhao Source Type: research

Hepatoprotective Effect of Annulohypoxylon stygium Melanin on Acute Alcoholic Liver Injury in Mice
In this study, we researched the effects of AsM on the protection against acute liver injury in mice and its mechanism. The results showed that AsM had no significant effect on body weight in mice but reduced the liver index. It was able to significantly decrease the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), the contents of triglyceride (TG) and total cholesterol (TC) in mice. Simultaneously, it raised the levels of superoxide dismutase (SOD), peroxidase (CAT), and glutathione peroxidase (GSH-Px), which obviously exceeded those of the EtOH group. AsM could significantly lower the le...
Source: Applied Biochemistry and Biotechnology - February 21, 2024 Category: Biochemistry Authors: Ruofan Liu Cuiyuan Mo Xuetuan Wei Aimin Ma Source Type: research

Enhancement of Visible Light Antibacterial Activities of Cellulose Fibers from Lotus Petiole Decorated ZnO Nanoparticles
In this study, cellulose fibers extracted from lotus petioles were utilized as a substrate and decorated with various shapes of ZnO nanoparticles (NPs), including small bean, hexagonal ingot-like, long cylindrical, and hexagonal cylinder-shaped NPs. Increasing zinc salt molar concentration resulted in highly crystalline ZnO NPs forming and enhanced interactions between ZnO NPs and -OH groups of cellulose. The thermal stability and UV-visible absorption properties of the CZ samples were influenced by ZnO concentration. Notably, at a ZnO molar ratio of 0.1, the CZ 0.1 sample demonstrated the lowest weight loss, while the opt...
Source: Applied Biochemistry and Biotechnology - February 21, 2024 Category: Biochemistry Authors: Ngoc Hong Nguyen Truong Phi Le Trung Bao Ngoc Duong Vien Ky Le Hau Huu Do Ho Linh Ho Thuy Nguyen Tan Le Hoang Doan Ngoc Xuan Dat Mai Lan My Thi Nguyen Ngoc Kim Pham Source Type: research

Engineered the Active Site of ω-Transaminase for Enhanced Asymmetric Synthesis Towards (S)-1-[4-(Trifluoromethyl)phenyl]ethylamine
This study provides an efficient strategy for the engineering of ω-TA based on semi-rational approach and has the potential for the molecular modification of other biocatalysts.PMID:38381312 | DOI:10.1007/s12010-024-04886-7 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - February 21, 2024 Category: Biochemistry Authors: Zhi-Wen Duan Yao-Wu Wang Da-Dong Shen Xin-Qiang Sun Pu Wang Source Type: research

Enhanced Production of Sisomicin in Micromonospora inyoensis by Protoplast Mutagenesis and Fermentation Optimization
This study not only provides a reference for further breeding of high-yielding strains and fermentation optimization, but also enhances our understanding of sisomicin production.PMID:38381313 | DOI:10.1007/s12010-024-04889-4 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - February 21, 2024 Category: Biochemistry Authors: Jianguo Xu Shulin Shen Zhehua Hu Guoqiang Xu Hui Li Zhenghong Xu Jinsong Shi Source Type: research