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Toxic Impact of Sheathmar on Oreochromis mossambicus: A Comprehensive Biomarker Analysis
This study assessed the toxicity of the Sheathmar pesticide on Oreochromis mossambicus. The 96-h median lethal concentration (LC50) was determined using probit analysis, with survivability assessed using general threshold survival models (GUTS). Over 45 days, fish exposed to sublethal Sheathmar doses were studied for changes in growth, blood profiles, and liver enzymes. According to the findings, the LC50 values of Sheathmar for Oreochromis mossambicus at different time intervals were determined as follows: 3016.64 µg/l at 24 h, 2723.13 µg/l at 48 h, 2415.45 µg/l at 72 h, and 2154.87 µg/l at 96 h. The GUTS-SD model eff...
Source: Applied Biochemistry and Biotechnology - March 13, 2024 Category: Biochemistry Authors: Sharmistha Medda Ritwick Bhattacharya Sarmila Pal Nimai Chandra Saha Source Type: research

Bone Marrow-Derived C-Kit < sup > + < /sup > Cells Improved Inflammatory IL-33/ST-2/ILC2 Axis in the Lung Tissue of Type 2 Diabetic Rats
This study evaluated the effect of BM-derived C-kit+ cells on the inflammation signaling pathway in lung tissue of type 2 diabetic male rats. Ten rats were used to extract C-kit cells, and 48 male Wistar rats weighing 180 ± 20 g were randomly divided into four equal groups: (1) Control (Cont), (2) Diabetic (D), (3) Diabetic + C-kit+ cells (D + C-kit pos) intravenously injected 50-µl phosphate buffer saline (PBS) containing 300,000 C-kit+ cells, and (4) Diabetic + C-kit- cells (D + C-kit neg), intravenously injected C-kit- cells. Diabetes induction increased IL-33, ST-2, CD127, and IL-2 levels and decreased IL-10. C-kit+ ...
Source: Applied Biochemistry and Biotechnology - March 13, 2024 Category: Biochemistry Authors: Milad Mohammadzadeh Seyed Zanyar Athari Fariba Ghiasi Rana Keyhanmanesh Arshad Ghaffari-Nasab Leila Roshangar Elnaz Salmani Korjan Aref Delkhosh Fariba Mirzaei Bavil Source Type: research

Causal Association of Telomere Length and Loss of Bone: a Directional Mendelian Randomization Study of Multi-Outcomes
This study employed a genome-wide association study (GWAS) to investigate the relationship between telomere length and marginal bone loss (MBL), a marker of bone health and aging. Telomere length, a biological indicator of aging, was analyzed alongside several serum markers of bone loss. Following a screen for appropriate instrumental variables, telomere length was designated as the exposure variable. We conducted the main analysis using random-effects inverse variance weighting (IVW) and supplemented it with MR Egger, weighted median, simple mode, and weighted mode analyses, employing a total of five methods. Positive out...
Source: Applied Biochemistry and Biotechnology - March 13, 2024 Category: Biochemistry Authors: Xiaoxun Du Cunliang Guo Chao Zhang Baoshan Xu Source Type: research

Understanding the Dynamics of Human Defensin Antimicrobial Peptides: Pathogen Resistance and Commensal Induction
Appl Biochem Biotechnol. 2024 Mar 13. doi: 10.1007/s12010-024-04893-8. Online ahead of print.ABSTRACTAntimicrobial peptides (AMPs), also known as host defense peptides, are petite molecules with inherent microbicidal properties that are synthesized by the host's innate immune response. These peptides serve as an initial barrier against pathogenic microorganisms, effectively eliminating them. Human defensin (HD) AMPs represent a prominent group of peptides involved in the innate immune response of humans. These peptides are primarily produced by neutrophils and epithelial cells, serving as a crucial defense mechanism agains...
Source: Applied Biochemistry and Biotechnology - March 13, 2024 Category: Biochemistry Authors: Veenayohini Kumaresan Yoganathan Kamaraj Satheeshkumar Subramaniyan Ganesh Punamalai Source Type: research

Bioinformatics Analysis and Experimental Validation for Exploring Key Molecular Markers for Glioblastoma
This study aims to explore novel biomarkers for GBM via bioinformatics analysis and experimental verification. Dataset GSE103229 was obtained from the GEO database to search differentially expressed lncRNA (DELs), mRNAs (DEMs), and miRNAs (DEMis). Hub genes were selected to establish competing endogenous RNA (ceRNA) networks. The GEPIA database was employed for the survival analysis and expression detection of hub genes. Hub gene expression in GBM tissue samples and cell lines was validated using RT-qPCR. Western blotting was employed for protein expression evaluation. SYT1 overexpression vector was transfected in GBM cell...
Source: Applied Biochemistry and Biotechnology - March 6, 2024 Category: Biochemistry Authors: Zhenchao Huang Zhijie Chen En'peng Song Peng Yu Weiwen Chen Huiqin Lin Source Type: research

Investigation of In Vitro Anti-cancer and Apoptotic Potential of Onion-Derived Nanovesicles Against Prostate and Cervical Cancer Cell Lines
This study embarks on the first-time investigation of the anti-cancerous effect of onion-derived nanovesicles (ODNVs). ODNVs were isolated employing differential centrifugation followed by ultracentrifugation and subsequent characterization using dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectroscopy (FTIR). Furthermore, we delineated the anti-cancerous effect of ODNVs on two cancer cell line models HeLa (cervical cancer) and PC-3 (prostate cancer) using MTT assay, DAPI-based DNA damage using immunofluorescence microscopy, colony formation assay, mig...
Source: Applied Biochemistry and Biotechnology - March 5, 2024 Category: Biochemistry Authors: Vinayak Sharma Eshu Singhal Sinha Jagtar Singh Source Type: research

A Method Based on a Modified Fluorescence In Situ Hybridization (FISH) Approach for the Sensing of Staphylococcus aureus from Nasal Samples
Appl Biochem Biotechnol. 2024 Feb 29. doi: 10.1007/s12010-024-04892-9. Online ahead of print.ABSTRACTStaphylococcus aureus is a major source of bacteremia and develops several complications, causing high morbidity and mortality. Rapid identification and detection of these bacteria have become an important issue for biomedical applications. Herein, an optical method based on a modified fluorescence in situ hybridization (FISH) approach has been established using DNA hybridization technology for the swift detection of pathogenic S. aureus from clinical samples. The platform was constructed with single-stranded genomic DNA an...
Source: Applied Biochemistry and Biotechnology - February 29, 2024 Category: Biochemistry Authors: Amtul Jamil Sami Sehrish Bilal Sadaf Alam Madeeha Khalid Hammad Ahmad Mangat Source Type: research

Expression and Immobilization of Tannase for Tannery Effluent Treatment from Lactobacillus plantarum and Staphylococcus lugdunensis: A Comparative Study
Appl Biochem Biotechnol. 2024 Feb 29. doi: 10.1007/s12010-024-04861-2. Online ahead of print.ABSTRACTAgro-industrial discharges have higher concentrations of tannins and have been a significant cause of pollution to water bodies and soil surrounding the agro-industries. So in this study, toxic tannic acid is into commercially valuable gallic acid from the tannery effluent using immobilized microbial tannase. Tannase genes were isolated from Lactobacillus plantarum JCM 1149 (tanLpl) and Staphylococcus lugdunensis MTCC 3614 (tanA). Further, these isolated tannese genes were cloned and expressed in BL 21 host using pET 28a as...
Source: Applied Biochemistry and Biotechnology - February 29, 2024 Category: Biochemistry Authors: Amballa Chaitanyakumar Prathap Somu Ramachandran Srinivasan Source Type: research

Synthesis of Bimetallic Palladium/Zinc Oxide Nanocomposites Using Crocus sativus and Its Anticancer Activity via the Induction of Apoptosis in Cervical Cancer
In this study, a straightforward and environmentally friendly method for creating bimetallic nanoparticles (NPs) by combining palladium (Pd) and zinc oxide (ZnO) using Crocus sativus extract (CS-Pd/ZnO NCs) was reported; the bio-synthesize bimetallic palladium/zinc oxide nanocomposites and their antioxidant and anti-cancer properties were assessed. The developed Pd/ZnO NPs were characterized using different approaches, including UV-vis, DLS, FTIR, EDX, and SEM analyses. The present investigation shows how nanocomposites are made, their distinctive properties, antioxidant activity, anticancer mechanisms, and their potential...
Source: Applied Biochemistry and Biotechnology - February 29, 2024 Category: Biochemistry Authors: Jun Feng Leilei He Jin Qing Hui Krishnamoorthy Kavithaa Zhengzheng Xu Source Type: research

Correction to: Ginkgo biloba Extract 50 (GBE50) Exerts Antifibrotic and Antioxidant Effects on Pulmonary Fibrosis in Mice by Regulating Nrf2 and TGF- β1/Smad Pathways
Appl Biochem Biotechnol. 2024 Feb 29. doi: 10.1007/s12010-024-04852-3. Online ahead of print.NO ABSTRACTPMID:38421573 | DOI:10.1007/s12010-024-04852-3 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - February 29, 2024 Category: Biochemistry Authors: Wei Liang Hongmei Yang Ling Pan Sizun Wei Zhanhua Li Pengfei Zhang Ruixiang Li Yangcong Wu Maohua Liu Xiaohong Liu Source Type: research