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Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA
In this study, we attempted to display the surface of the PET-degrading enzyme, PETase, onto Escherichia coli using the membrane anchor, PgsA, from Bacillus subtilis to omit the need for purification of the enzyme. Immunofluorescence staining confirmed that PETase was successfully displayed on the surface of E. coli cells when a fusion of PgsA and PETase was expressed. The surface-displaying E. coli was able to degrade 94.6% of 1 mM bis(2-hydroxyethyl) terephthalate in 60 min, and the PET films were also degraded in trace amounts. These results indicate that PgsA can be used to present active PETase on the cell surface of ...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Takuma Yamashita Takuya Matsumoto Ryosuke Yamada Hiroyasu Ogino Source Type: research

Proteomic Analysis of Collagen: a Mass Spectrometry Approach to Material Identification of Shadow Puppet Cultural Relics
Appl Biochem Biotechnol. 2024 Jan 2. doi: 10.1007/s12010-023-04822-1. Online ahead of print.ABSTRACTShadow puppets are a popular art form in various regions, including China, Indonesia, and Turkey, and are rich in cultural significance. However, there is a considerable lack of research on the materials, diseases and conservation techniques related to shadow puppet relics. Material identification is the basis for understanding the production process of ancient shadow puppet relics and evaluating their deterioration degree. The microscopic morphology and infrared spectroscopy results in our experiments showed that the tradit...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Yangbo Duan Muzi Zhang Chen Min Yalun Lin Li Li Source Type: research

Inhibition of Integrin-Associated Kinases FAK and ILK on the In Vitro Model of Skin Wound Healing
Appl Biochem Biotechnol. 2024 Jan 2. doi: 10.1007/s12010-023-04842-x. Online ahead of print.ABSTRACTDermal fibroblasts are essential cells of skin tissue responsible for its normal functioning. In skin wounds, the differentiation of resident fibroblasts into myofibroblasts occurs, which is accompanied by the rearrangement of actin cytoskeleton with the expression of alpha-smooth muscle actin. This transformation is a prerequisite for a successful wound healing. At the same time, different studies indicate that extracellular matrix may be involved in regulation of this process. Since the connection between cells and matrix ...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Natalya Bildyug Source Type: research

An Integrated Computational Approaches for Designing of Potential Piperidine based Inhibitors of Alzheimer Disease by Targeting Cholinesterase and Monoamine Oxidases Isoenzymes
Appl Biochem Biotechnol. 2024 Jan 2. doi: 10.1007/s12010-023-04815-0. Online ahead of print.ABSTRACTThe study aimed to evaluate the potential of piperidine-based 2H chromen-2-one derivatives against targeted enzymes, i.e., cholinesterase's and monoamine oxidase enzymes. The compounds were divided into three groups, i.e., 4a-m ((3,4-dimethyl-7-((1-methylpiperidin-4-yl)oxy)-2H-chromen-2-one derivatives), 5a-e (3,4-dimethyl-7-((1-methypipridin-3-yl)methoxy)-2H-chromen-2-one derivatives), and 7a-b (7-(3-(3,4-dihydroisoquinolin-2(1H)-yl)propoxy)-3,4-dimethyl-2H-chromen-2-one derivatives) with slight difference in the basic stru...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Muhammad Sarfraz Muhammad Khurrum Ibrahim Syeda Abida Ejaz Hafiz Muhammad Attaullah Mubashir Aziz Mosab Arafat Tahira Shamim Muawya Elhadi Tahira Ruby Hafiz Kashif Mahmood Source Type: research

Effects of ADSC-Derived Exosome LRRC75A-AS1 on Anti-inflammatory Function After SCI
This study aimed to examine how adipose-derived mesenchymal stem cell exosomes (ADSC-exos) can be used to treat spinal cord injury. We analysed differentially expressed mRNAs in SCI using bioinformatics data, gene expression profiles in inflammatory cell models, RT-qPCR and WB. Apoptosis was detected with flow cytometry. Starbase provides the control mechanism for FDFT1. Target interactions were detected with dual-luciferase reporter and RIP assays. Exosomes were isolated from adipose tissue-derived mesenchymal stem cells and subsequently characterized with western blot analysis, transmission electron microscopy and nanopa...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Xiaohui Xing Peng Xu Xiaoyang Xing Zhentao Xu Zhen Huang Zhongchen Li Xueyuan Li Yilei Xiao Source Type: research

Erianin Impedes the Proliferation and Metastatic Migration Through Suppression of STAT-3 Phosphorylation in Human Esophageal Cancer Cells
In this study, we have investigated erianin, a natural phenolic drug that impedes proliferation and metastatic migration through suppression of STAT-3 phosphorylation in human esophageal cancer cells. Eca-109 cells were treated with different concentrations of erianin (4, 8, 12 µM) for 24 h, and then cell proliferation, apoptosis, and metastatic markers were evaluated. Erianin-induced cytotoxicity and cell proliferation were examined using MTT and crystal violet staining techniques. The measurement of reactive oxygen species (ROS) and the study of apoptotic changes were conducted through flow cytometry. Furthermore, prote...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Anxi Hu Kunkun Li Source Type: research