Enhancing L-Asparagine Bioproduction Efficiency Through L-Asparagine Synthetase and Polyphosphate Kinase-Coupled Conversion and ATP Regeneration
This study aimed to address the challenges associated with biopreparation of L-asparagine. Firstly, the functionality and characteristics of recombinant L-asparagine synthetase enzymes derived from Escherichia coli and Lactobacillus salivarius were evaluated to determine their practical applicability. Subsequently, recombinant expression of polyphosphate kinase from Erysipelotrichaceae bacterium was conducted. A reaction system for L-asparagine synthesis was established using a dual enzyme-coupled conversion approach. Under the optimal reaction conditions, a maximum yield of 11.67 g/L of L-asparagine was achieved, with an ...
Source: Applied Biochemistry and Biotechnology - February 15, 2024 Category: Biochemistry Authors: Zijia Wei Yuhua Zhang Xuguo Duan Yucheng Fan Source Type: research

Enhancing L-Asparagine Bioproduction Efficiency Through L-Asparagine Synthetase and Polyphosphate Kinase-Coupled Conversion and ATP Regeneration
This study aimed to address the challenges associated with biopreparation of L-asparagine. Firstly, the functionality and characteristics of recombinant L-asparagine synthetase enzymes derived from Escherichia coli and Lactobacillus salivarius were evaluated to determine their practical applicability. Subsequently, recombinant expression of polyphosphate kinase from Erysipelotrichaceae bacterium was conducted. A reaction system for L-asparagine synthesis was established using a dual enzyme-coupled conversion approach. Under the optimal reaction conditions, a maximum yield of 11.67 g/L of L-asparagine was achieved, with an ...
Source: Applied Biochemistry and Biotechnology - February 15, 2024 Category: Biochemistry Authors: Zijia Wei Yuhua Zhang Xuguo Duan Yucheng Fan Source Type: research

Lactobacillus rhamnosus and Lactobacillus delbrueckii Ameliorate the Expression of miR-125a and miR-146a in Systemic Lupus Erythematosus Patients
Appl Biochem Biotechnol. 2024 Feb 14. doi: 10.1007/s12010-023-04827-w. Online ahead of print.ABSTRACTThe microRNAs are non-coding RNA molecules involved in physiological and pathological processes, causing autoimmune diseases such as systemic lupus erythematosus (SLE). Probiotics are living microorganisms that possess beneficial effects on the host immune system and modulate it. The effect of Lactobacillus rhamnosus and Lactobacillus delbrueckii on the expression of miR-125a and miR-146a was studied in peripheral blood mononuclear cells (PBMCs) from newly diagnosed lupus patients in this in vitro study. During this study, ...
Source: Applied Biochemistry and Biotechnology - February 14, 2024 Category: Biochemistry Authors: Zohreh Vahidi Effat Saghi Mahmoud Mahmoudi Zahra RezaieYazdi Seyed-Alireza Esmaeili Fariba Zemorshidi Morteza Samadi Maryam Rastin Source Type: research

Role of Structural Peculiarities of Flavonoids in Suppressing AGEs Generated From HSA/Glucose System
Appl Biochem Biotechnol. 2024 Feb 14. doi: 10.1007/s12010-023-04844-9. Online ahead of print.ABSTRACTThe pathogenesis of diabetes is related to the amount of advanced glycation end products (AGEs) that are naturally generated from the attachment of glucose with tissue and circular proteins. Human serum albumin (HSA) is more susceptible to AGE occurrence than other circular proteins due to its sensitive sites and high abundance. Considering the location of hydroxyl groups in the structure of flavonoids, which play a major role in suppressing of AGEs generating pathways, the present study was conducted to compare the effect ...
Source: Applied Biochemistry and Biotechnology - February 14, 2024 Category: Biochemistry Authors: Alireza Mohammadpour Morteza Sadeghi Mehran Miroliaei Source Type: research

Development of a Competitive Chemiluminescent Assay for Quantitative Determination of TP53 Fusion Protein Using Reagent Strips
In this study, the reagent strip method was initially used to detect TP53 fusion protein by combining the advantages of NSP-DMAE-NHS and immunochromatography. In our experiment, the control and study lines on the strips were immobilized through HRP-conjugated goat anti-rabbit IgG and TP53 antigen, respectively. The optimized concentration of the anti-TP53 antibody-NSP-DMAE-NHS immunoconjugates was then added to the TP53 antigen samples. After, the test strips were inserted and left in the aforementioned buffer solution for an additional 20 min. Finally, a lab-made luminous measurement device was used to analyze the corresp...
Source: Applied Biochemistry and Biotechnology - February 14, 2024 Category: Biochemistry Authors: Linyu Wang Zhifang He Jianye Li Source Type: research

Targeting Efficient Features of Urate Oxidase to Increase Its Solubility
Appl Biochem Biotechnol. 2024 Feb 3. doi: 10.1007/s12010-023-04819-w. Online ahead of print.ABSTRACTWith the demand for mass production of protein drugs, solubility has become a serious issue. Extrinsic and intrinsic factors both affect this property. A homotetrameric cofactor-free urate oxidase (UOX) is not sufficiently soluble. To engineer UOX for optimum solubility, it is important to identify the most effective factor that influences solubility. The most effective feature to target for protein engineering was determined by measuring various solubility-related factors of UOX. A large library of homologous sequences was ...
Source: Applied Biochemistry and Biotechnology - February 3, 2024 Category: Biochemistry Authors: Mohammad Reza Rahbar Navid Nezafat Mohammad Hossein Morowvat Amir Savardashtaki Mohammad Bagher Ghoshoon Kamran Mehrabani-Zeinabad Younes Ghasemi Source Type: research

Explore Key Genes and Mechanisms Involved in Colon Cancer Progression Based on Bioinformatics Analysis
In this study, 266 common DEGs were obtained from the intersection of two datasets. The GO analysis suggested the common DEGs mainly participated in the one-carbon metabolic process, cell cycle G2/M phase transition, organelle fission, cell cycle phase transition regulation, and regulation of mitotic cell cycle phase transition. The KEGG analysis demonstrated the common DEGs were related to the p53 signaling pathway, nitrogen metabolism, mineral absorption, and cell cycle. 10 hub genes including CCNB1, KIF4A, TPX2, MT1F, PRC1, PLK4, CALD1, MMP9, CLCA1, and MMP1 were identified and CCNB1, CLCA1, and PLK4 were prognosis-rela...
Source: Applied Biochemistry and Biotechnology - January 31, 2024 Category: Biochemistry Authors: Yongting Lan Xiuzhen Yang Yulian Wei Zhaobing Tian Lina Zhang Jian Zhou Source Type: research

Mesenchymal Stem Cell-Induced Neuroprotection in Pediatric Neurological Diseases: Recent Update of Underlying Mechanisms and Clinical Utility
Appl Biochem Biotechnol. 2024 Jan 23. doi: 10.1007/s12010-023-04752-y. Online ahead of print.ABSTRACTPediatric neurological diseases refer to a group of disorders that affect the nervous system in children. These conditions can have a significant impact on a child's development, cognitive function, motor skills, and overall quality of life. Stem cell therapy is a new and innovative approach to treat various neurological conditions by repairing damaged neurons and replacing those that have been lost. Mesenchymal stem cells (MSCs) have gained significant recognition in this regard due to their ability to differentiate into d...
Source: Applied Biochemistry and Biotechnology - January 23, 2024 Category: Biochemistry Authors: Wei Chen Qiaoling Ren Junchen Zhou Wenchun Liu Source Type: research

Melitoxin Inhibits Proliferation, Metastasis, and Invasion of Glioma U251 Cells by Down-regulating F2RL1
This study was aimed to investigate the effect of melittin in glioma and explore whether F2RL1 is closely involved in glioblastoma cells proliferation. TCGA and GES databases were used to evaluate the role of F2RL1 in gliomas. The U251 cells were divided into a control lentivirus + PBS group (NC-PBS), F2RL1 intervention lentivirus + PBS group (KD-PBS), control lentivirus + melittin group (NC-melittin), and F2RL1 intervention lentivirus + melittin group (KD-melittin). Cell proliferation was detected by MTT and EDU staining assays. The apoptosis rate was assessed by flow cytometry. Expressions of genes related to apoptosis, ...
Source: Applied Biochemistry and Biotechnology - January 22, 2024 Category: Biochemistry Authors: Peng Xu Jie Zhou Xiaohui Xing Yuan Hao Mingxu Gao Zhongchen Li Xin Li Mengyou Li Yilei Xiao Source Type: research

Plasma MCP-1 and TGF- β1 Levels are Associated with Kidney Injury in Children with Congenital Anomalies of the Kidney and Urinary Tract
Appl Biochem Biotechnol. 2024 Jan 20. doi: 10.1007/s12010-023-04808-z. Online ahead of print.ABSTRACTCongenital anomalies of the kidney and urinary tract (CAKUT) are primarily causal for end-stage renal disease and have significant implications for long-term survival. A total of 39 healthy controls and 94 children with chronic kidney disease (CKD) were enrolled (3-12 years old as children, 13-18 years old as adolescents), who were divided into CAKUT and Non-CAKUT according to the etiology of CKD. CKD group was further classified according to estimating glomerular filtration rate (eGFR). Circulating levels of inflammatory m...
Source: Applied Biochemistry and Biotechnology - January 20, 2024 Category: Biochemistry Authors: XiaoQi Xuan Xiao Pu Yue Yang JinLong Yang YongLe Li Hang Wu JianGuo Xu Source Type: research

Waste Sludge: Entirely Waste or a Sustainable Source of Biocrude? A Review
Appl Biochem Biotechnol. 2024 Jan 18. doi: 10.1007/s12010-023-04846-7. Online ahead of print.ABSTRACTBiomass-derived biocrude is gaining greater recognition from people in general as an alternative fuel source to traditional fossil fuels. Worldwide, a great deal of research is being done to develop fuels made from sustainable biomass in order to replace the current conventional energy sources. Waste sludge has been thought of as a viable raw biomass source because of its accessibility, affordability, high lignin content, and higher heating value. Additionally, considering sludge contains a high proportion of moisture and w...
Source: Applied Biochemistry and Biotechnology - January 18, 2024 Category: Biochemistry Authors: Farin Tasnuva Dhara Md Atik Fayshal Source Type: research

Th9 Cytokines Inhibit Proliferation, Promote Apoptosis, and Immune Escape in Thyroid Carcinoma Cells
Appl Biochem Biotechnol. 2024 Jan 15. doi: 10.1007/s12010-023-04821-2. Online ahead of print.ABSTRACTTo investigate the regulatory effects of T helper 9 (Th9) cytokines on the proliferation, apoptosis and immune escape of thyroid cancer cells. The survival rate of human thyroid cancer cell line TPC-1 after treatment with 0, 1, 2.5, 5, 10, 20 ng/ml IL-9 (or IL-21) was determined by CCK-8 method and suitable concentrations of IL-9 and IL-21 were screened out. The TPC-1 cells cultured in vitro were randomly grouped into control group, IL-9 group, IL-21 group and IL-9+IL-21 group. After treatment with IL-9 and IL-21 factors, t...
Source: Applied Biochemistry and Biotechnology - January 15, 2024 Category: Biochemistry Authors: Xinyu Ying Xinyi Ma Ziru Yang Bo Zhou Source Type: research

Characterization of Collagen from Jellyfish Aurelia aurita and Investigation of Biomaterials Potentials
Appl Biochem Biotechnol. 2024 Jan 15. doi: 10.1007/s12010-023-04848-5. Online ahead of print.ABSTRACTMarine collagen sources are potent alternatives due to abundant yield, low pathogen infection risk, high biocompatibility, and any religious and ethical restrictions compared to terrestrial collagen sources. In this research, we aim to investigate the biomaterials potential of the collagen from Aurelia aurita, which is a native jellyfish species in the Marmara Sea. Spectroscopic techniques were used to investigate the structure of jellyfish collagen (JCol) from acid-soluble fraction and compared to Jellagen® from Rhizostom...
Source: Applied Biochemistry and Biotechnology - January 15, 2024 Category: Biochemistry Authors: Elif Balikci Erkan T ürker Baran Aydin Tahmasebifar Bengi Yilmaz Source Type: research

Treatment of Intracerebral Hemorrhage with Traditional Chinese Medicine Monomer Wogonin by Modifying NLRP3 with METTL14 to Inhibit Neuronal Cell Pyroptosis
Appl Biochem Biotechnol. 2024 Jan 15. doi: 10.1007/s12010-023-04849-4. Online ahead of print.ABSTRACTThe aim of this study was to investigate the alleviating effect of wogonin on intracerebral hemorrhage (ICH) and its mechanism. The hemin-treated PC-12 cells were constructed to mimic ICH in vitro. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis was used for cell viability measurement and flow cytometry was for pyroptosis detection. Enzyme-linked immunosorbent assay (ELISA) assay and western blot were used to detect the protein levels of pyroptosis-related proteins. The modification level of N6-m...
Source: Applied Biochemistry and Biotechnology - January 15, 2024 Category: Biochemistry Authors: Libo Li Jinbing Gong Wenjia Zhang Source Type: research

Melatonin as an Ameliorative Agent Against Cadmium- and Lead-Induced Toxicity in Fish: an Overview
Appl Biochem Biotechnol. 2024 Jan 15. doi: 10.1007/s12010-023-04723-3. Online ahead of print.ABSTRACTDiverse anthropogenic activities and lack of knowledge on its consequences have promoted serious heavy metal contaminations in different aquatic systems throughout the globe. The non-biodegradable nature of most of these toxic heavy metals has increased the concern on their possible bioaccumulation in aquatic organisms as well as in other vertebrates. Among these aquatic species, fish are most sensitive to such contaminated water that not only decreases their chance of survivability in the nature but also increases the prob...
Source: Applied Biochemistry and Biotechnology - January 15, 2024 Category: Biochemistry Authors: Ashis Kumar Panigrahi Palash Kumar Pal Dipanwita Sarkar Paria Source Type: research