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Bacterial nano-factories as a tool for the biosynthesis of TiO < sub > 2 < /sub > nanoparticles: characterization and potential application in wastewater treatment
Appl Biochem Biotechnol. 2024 Jan 4. doi: 10.1007/s12010-023-04839-6. Online ahead of print.ABSTRACTThe development of reliable and eco-conscious processes for nanoparticle synthesis constitutes a significant element in nanotechnology. TiO2 nanoparticles (NPs) are becoming essential due to their potential uses in dentistry, surgery, agriculture, and pharmacy. This leads to the development of various procedures for producing TiO2 NPs using various physicochemical methods. Still, the drawbacks of these conventional methods are associated with the emission of toxic chemicals into the atmosphere and high energy demands in prod...
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Zahra Latifi Azizi Sara Daneshjou Source Type: research

Luffa cylindrica (Sponge Gourd) Fibers in Treatment of Greywater: an Aerobic Fixed-Film Reactor Approach
In this study, a fixed-film reactor was designed with Luffa cylindrica (an annually occurring fruit) as a bio-carrier. The lab-scale reactors were configured with and without Luffa cylindrica and were run for 90 days in fed-batch mode. Scanning electron microscopy (SEM) was performed to validate biofilm production over time. Monitoring COD, nitrogen, and total phosphate removal allowed for analysis of treatment effectiveness. Results demonstrated the treatment efficiency for the experimental reactor was 70.96%, 97.02%, 92.57%, and 81.20% for COD, nitrogen, phosphate, and anionic surfactant (AS), respectively. 16 s rRNA gen...
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Upasana Arora Himanshu Kumar Khuntia H N Chanakya Atya Kapley Source Type: research

Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions
In conclusion, RNAi remains a versatile and pivotal tool in modern plant research, offering novel avenues for understanding gene functions and improving crop traits. Its integration with other biotechnological approaches such as gene editing holds the potential to shape the future of agriculture and sustainable food production.PMID:38175411 | DOI:10.1007/s12010-023-04850-x (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Divya Chaudhary Anand Singh Jeena None Rohit Sonali Gaur Rishi Raj Shefali Mishra None Kajal Om Prakash Gupta Mintu Ram Meena Source Type: research

PICALM as a Novel Prognostic Biomarker and Its Correlation with Immune Infiltration in Breast Cancer
Appl Biochem Biotechnol. 2024 Jan 4. doi: 10.1007/s12010-023-04840-z. Online ahead of print.ABSTRACTPICALM (phosphatidylinositol-binding clathrin assembly protein) mutations have been linked to a number of human disorders, including leukemia, Alzheimer's disease, and Parkinson's disease. Nevertheless, the effect of PICALM on cancer, particularly on prognosis and immune infiltration in individuals with BRCA, is unknown. We obtained the data of breast cancer patients from The Cancer Genome Atlas (TCGA) database, and analyzed the expression of PICALM in breast cancer, its impact on survival' and its role in tumor immune invas...
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Naer A Pengfei Lyu Yue Yu Meiling Liu Shaohua Cheng Meiyan Chen Yunhong Liu Xuchen Cao Source Type: research

Biosynthesis of Zinc Oxide Nanoparticles Using Bacillus paramycoides for In Vitro Biological Activities and In Vivo Assessment Against Hepatorenal Injury Induced by CCl < sub > 4 < /sub > in Rats
In conclusion, ZnONPs were successfully synthesized from the terrestrial Bacillus paramycoides and recorded in vitro antioxidant, anticancer, and antibacterial effects as well as in vivo anti-hepatorenal toxicity effects.PMID:38175413 | DOI:10.1007/s12010-023-04817-y (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Heba A El-Refai Alaa M Saleh Shimaa I A Mohamed Asmaa F Aboul Naser Rania A Zaki Sanaa K Gomaa Manal A Hamed Source Type: research

Rosmarinic Acid Liposomes Downregulate Hepcidin Expression via BMP6-SMAD1/5/8 Pathway in Mice with Iron Overload
The objective of this study is to examine the potential protective effect of rosmarinic acid (RosA) encapsulated within nanoliposomes (RosA-LIP) on hepatic damage induced by iron overload. The characteristics, stability, and release of RosA-LIP in vitro were identified. The mice were randomly assigned to five groups: Control, Model, Model+DFO (DFO), Model+RosA (RosA), and Model+RosA-LIP (RosA-LIP). The iron overload model was induced by administering iron dextran (i.p.). The DFO, RosA, and RosA-LIP groups received iron dextran and were subsequently treated with DFO, RosA, and RosA-LIP for 14 days. We developed a novel form...
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Cui-Ling Jia Bo-Liang Li Zi-Hao Zhao Zhi Zhang None Qi-Chen Ji-Xian Song Yujing Gou Si-Yu Gao Chen-Sha Sun Yingna He En-Sheng Ji Yashuo Zhao Source Type: research

Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes
Appl Biochem Biotechnol. 2024 Jan 4. doi: 10.1007/s12010-023-04838-7. Online ahead of print.ABSTRACTIn recent years, enzyme immobilization technology has been developed, and studies on immobilized enzyme materials have become very prominent. With the immobilization technique, enzymes and compatible carrier materials are combined or enzyme crystals/aggregates are used in a carrier-free fashion, by physical, chemical, or biochemical methods. As a kind of biocatalyst, immobilized enzymes can catalyze certain chemical reactions with high selectivity and high efficiency under relatively mild reaction conditions and eliminate po...
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Yong Jiang Jinxia Zheng Mengna Wang Wanqi Xu Yiquan Wang Li Wen Jian Dong Source Type: research

ω-3PUFAs Inhibit Hypoxia-Induced Retinal Neovascularization via Regulating Microglial Pyroptosis through METTL14-Mediated m6A Modification of IFNB1 mRNA
This study offers a novel potential target for the treatment of retinal neovascular diseases.PMID:38175416 | DOI:10.1007/s12010-023-04795-1 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Shun Wang Jing Zhang Jun Chen Lanlan Tang Min Ke Yanni Xue Ying He Yan Gong Zhi Li Source Type: research

Pan-Cancer Analysis of P3H1 and Experimental Validation in Renal Clear Cell Carcinoma
In this study, for the first time, aspects associated with P3H1, such as the mRNA expression, any mutation, promoter methylation, and prognostic significance, the relationship between P3H1 and clinicopathological parameters, drug sensitivity, and immune cell infiltration were investigated by searching several databases including The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), cBioPortal, and The Tumor Immune Evaluation Resource (TIMER2.0) using bioinformatics tools. The findings indicate significant differential expression of P3H1 in most tumors when compared to normal tissues, with a strong association ...
Source: Applied Biochemistry and Biotechnology - January 4, 2024 Category: Biochemistry Authors: Yongjie Li Ting Wang Feng Jiang Source Type: research

Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA
In this study, we attempted to display the surface of the PET-degrading enzyme, PETase, onto Escherichia coli using the membrane anchor, PgsA, from Bacillus subtilis to omit the need for purification of the enzyme. Immunofluorescence staining confirmed that PETase was successfully displayed on the surface of E. coli cells when a fusion of PgsA and PETase was expressed. The surface-displaying E. coli was able to degrade 94.6% of 1 mM bis(2-hydroxyethyl) terephthalate in 60 min, and the PET films were also degraded in trace amounts. These results indicate that PgsA can be used to present active PETase on the cell surface of ...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Takuma Yamashita Takuya Matsumoto Ryosuke Yamada Hiroyasu Ogino Source Type: research

Proteomic Analysis of Collagen: a Mass Spectrometry Approach to Material Identification of Shadow Puppet Cultural Relics
Appl Biochem Biotechnol. 2024 Jan 2. doi: 10.1007/s12010-023-04822-1. Online ahead of print.ABSTRACTShadow puppets are a popular art form in various regions, including China, Indonesia, and Turkey, and are rich in cultural significance. However, there is a considerable lack of research on the materials, diseases and conservation techniques related to shadow puppet relics. Material identification is the basis for understanding the production process of ancient shadow puppet relics and evaluating their deterioration degree. The microscopic morphology and infrared spectroscopy results in our experiments showed that the tradit...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Yangbo Duan Muzi Zhang Chen Min Yalun Lin Li Li Source Type: research

Inhibition of Integrin-Associated Kinases FAK and ILK on the In Vitro Model of Skin Wound Healing
Appl Biochem Biotechnol. 2024 Jan 2. doi: 10.1007/s12010-023-04842-x. Online ahead of print.ABSTRACTDermal fibroblasts are essential cells of skin tissue responsible for its normal functioning. In skin wounds, the differentiation of resident fibroblasts into myofibroblasts occurs, which is accompanied by the rearrangement of actin cytoskeleton with the expression of alpha-smooth muscle actin. This transformation is a prerequisite for a successful wound healing. At the same time, different studies indicate that extracellular matrix may be involved in regulation of this process. Since the connection between cells and matrix ...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Natalya Bildyug Source Type: research

An Integrated Computational Approaches for Designing of Potential Piperidine based Inhibitors of Alzheimer Disease by Targeting Cholinesterase and Monoamine Oxidases Isoenzymes
Appl Biochem Biotechnol. 2024 Jan 2. doi: 10.1007/s12010-023-04815-0. Online ahead of print.ABSTRACTThe study aimed to evaluate the potential of piperidine-based 2H chromen-2-one derivatives against targeted enzymes, i.e., cholinesterase's and monoamine oxidase enzymes. The compounds were divided into three groups, i.e., 4a-m ((3,4-dimethyl-7-((1-methylpiperidin-4-yl)oxy)-2H-chromen-2-one derivatives), 5a-e (3,4-dimethyl-7-((1-methypipridin-3-yl)methoxy)-2H-chromen-2-one derivatives), and 7a-b (7-(3-(3,4-dihydroisoquinolin-2(1H)-yl)propoxy)-3,4-dimethyl-2H-chromen-2-one derivatives) with slight difference in the basic stru...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Muhammad Sarfraz Muhammad Khurrum Ibrahim Syeda Abida Ejaz Hafiz Muhammad Attaullah Mubashir Aziz Mosab Arafat Tahira Shamim Muawya Elhadi Tahira Ruby Hafiz Kashif Mahmood Source Type: research

Effects of ADSC-Derived Exosome LRRC75A-AS1 on Anti-inflammatory Function After SCI
This study aimed to examine how adipose-derived mesenchymal stem cell exosomes (ADSC-exos) can be used to treat spinal cord injury. We analysed differentially expressed mRNAs in SCI using bioinformatics data, gene expression profiles in inflammatory cell models, RT-qPCR and WB. Apoptosis was detected with flow cytometry. Starbase provides the control mechanism for FDFT1. Target interactions were detected with dual-luciferase reporter and RIP assays. Exosomes were isolated from adipose tissue-derived mesenchymal stem cells and subsequently characterized with western blot analysis, transmission electron microscopy and nanopa...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Xiaohui Xing Peng Xu Xiaoyang Xing Zhentao Xu Zhen Huang Zhongchen Li Xueyuan Li Yilei Xiao Source Type: research

Erianin Impedes the Proliferation and Metastatic Migration Through Suppression of STAT-3 Phosphorylation in Human Esophageal Cancer Cells
In this study, we have investigated erianin, a natural phenolic drug that impedes proliferation and metastatic migration through suppression of STAT-3 phosphorylation in human esophageal cancer cells. Eca-109 cells were treated with different concentrations of erianin (4, 8, 12 µM) for 24 h, and then cell proliferation, apoptosis, and metastatic markers were evaluated. Erianin-induced cytotoxicity and cell proliferation were examined using MTT and crystal violet staining techniques. The measurement of reactive oxygen species (ROS) and the study of apoptotic changes were conducted through flow cytometry. Furthermore, prote...
Source: Applied Biochemistry and Biotechnology - January 2, 2024 Category: Biochemistry Authors: Anxi Hu Kunkun Li Source Type: research