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Expression and Immobilization of Tannase for Tannery Effluent Treatment from Lactobacillus plantarum and Staphylococcus lugdunensis: A Comparative Study
Appl Biochem Biotechnol. 2024 Feb 29. doi: 10.1007/s12010-024-04861-2. Online ahead of print.ABSTRACTAgro-industrial discharges have higher concentrations of tannins and have been a significant cause of pollution to water bodies and soil surrounding the agro-industries. So in this study, toxic tannic acid is into commercially valuable gallic acid from the tannery effluent using immobilized microbial tannase. Tannase genes were isolated from Lactobacillus plantarum JCM 1149 (tanLpl) and Staphylococcus lugdunensis MTCC 3614 (tanA). Further, these isolated tannese genes were cloned and expressed in BL 21 host using pET 28a as...
Source: Applied Biochemistry and Biotechnology - February 29, 2024 Category: Biochemistry Authors: Amballa Chaitanyakumar Prathap Somu Ramachandran Srinivasan Source Type: research

Synthesis of Bimetallic Palladium/Zinc Oxide Nanocomposites Using Crocus sativus and Its Anticancer Activity via the Induction of Apoptosis in Cervical Cancer
In this study, a straightforward and environmentally friendly method for creating bimetallic nanoparticles (NPs) by combining palladium (Pd) and zinc oxide (ZnO) using Crocus sativus extract (CS-Pd/ZnO NCs) was reported; the bio-synthesize bimetallic palladium/zinc oxide nanocomposites and their antioxidant and anti-cancer properties were assessed. The developed Pd/ZnO NPs were characterized using different approaches, including UV-vis, DLS, FTIR, EDX, and SEM analyses. The present investigation shows how nanocomposites are made, their distinctive properties, antioxidant activity, anticancer mechanisms, and their potential...
Source: Applied Biochemistry and Biotechnology - February 29, 2024 Category: Biochemistry Authors: Jun Feng Leilei He Jin Qing Hui Krishnamoorthy Kavithaa Zhengzheng Xu Source Type: research

Correction to: Ginkgo biloba Extract 50 (GBE50) Exerts Antifibrotic and Antioxidant Effects on Pulmonary Fibrosis in Mice by Regulating Nrf2 and TGF- β1/Smad Pathways
Appl Biochem Biotechnol. 2024 Feb 29. doi: 10.1007/s12010-024-04852-3. Online ahead of print.NO ABSTRACTPMID:38421573 | DOI:10.1007/s12010-024-04852-3 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - February 29, 2024 Category: Biochemistry Authors: Wei Liang Hongmei Yang Ling Pan Sizun Wei Zhanhua Li Pengfei Zhang Ruixiang Li Yangcong Wu Maohua Liu Xiaohong Liu Source Type: research

Characterization, Anti-glycation, Anti-inflammation, and Lipase Inhibitory Properties of Rauvolfia vomitoria Leaf Extract: In Vitro and In Silico Evaluations for Obesity Treatment
Appl Biochem Biotechnol. 2024 Feb 28. doi: 10.1007/s12010-024-04865-y. Online ahead of print.ABSTRACTPancreatic lipase (PLP) is an enzyme responsible for the catalytic hydrolysis of fats and its inhibition is relevant for obesity management. Side effects linked with orthodox inhibitors have, however, paved the way for an increased search for safe natural sources. The present study investigated the anti-glycation, anti-inflammatory, and anti-lipase properties of Rauvolfia vomitoria aqueous (ARV), ethanolic (ERV), and methanolic (MRV) leaf extracts coupled with the molecular interactions of selected bioactive compounds with ...
Source: Applied Biochemistry and Biotechnology - February 28, 2024 Category: Biochemistry Authors: Akpovwehwee A Anigboro Oghenetega J Avwioroko Omoerere Oborirhovo Onoriode Akeghware Ernest U Durugbo Augustine Apiamu Victor I Olaoye Uchechukwu S Ezealigo Nyerhovwo J Tonukari Source Type: research

Display of Lignin Peroxidase on the Surface of Bacillus subtilis
In this study, the Escherichia coli-Bacillus subtilis (E. coli-B. subtilis) shuttle expression vector pHS-cotG-lip was constructed and displayed on the surface of Bacillus subtilis spores. The analysis of enzymatic properties showed that the optimal catalytic temperature and pH of the immobilized LiP were 55 °C and 4.5, respectively. Compared with free LiP (42 °C and pH4.0), the optimal reaction temperature increased by 13 °C. After incubation at 70 °C for 1 h, its activity remained above 30%, while the free LiP completely lost its activity under the same conditions. Adding Mn2+, DL-lactic acid, and PEG-4000 increased ...
Source: Applied Biochemistry and Biotechnology - February 27, 2024 Category: Biochemistry Authors: Shouzhi Li Lu He Na Shi Zhong Ni Quan Bu Daochen Zhu Huayou Chen Source Type: research

Recent Progress in Enzymatic Preparation of Chitooligosaccharides with a Single Degree of Polymerization and Their Potential Applications in the Food Sector
This article provides a comprehensive overview of emerging strategies for the enzymatic preparation of single COS, encompassing protein engineering, enzymatic membrane bioreactors, and transglycosylation reactions. Furthermore, the bioactivities of single COS, including anti-tumor, antioxidant, antibacterial, anti-inflammatory, and plant defense inducer properties, exhibit close associations with DP values. The potential applications of single COS, such as in functional food, food preservation, and crop planting, are also elucidated.PMID:38411934 | DOI:10.1007/s12010-024-04876-9 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - February 27, 2024 Category: Biochemistry Authors: Na Li Yuting Lu Xian Sheng Yi Cao Wei Liu Zhi Zhou Ling Jiang Source Type: research

High-Level Extracellular Expression of Hyaluronate Lyase HylP in Bacillus subtilis for Hyaluronan Degradation
In this study, HylP, an HA lyase from Streptococcus pyogenes phage (SPB) was successfully expressed in Bacillus subtilis. To improve the extracellular enzyme activity of HylP in B. subtilis, signal peptide engineering systematic optimization was carried out, and cultured it from shake flasks and fermenters, followed by purification, characterization, and analysis of degradation products. The results showed that the replacement of the signal peptide increased the extracellular enzyme activity of HylP from 1.0 × 104 U/mL to 1.86 × 104 U/mL in the shake flask assay, and using a 20 L fermenter in a batch fermentation process...
Source: Applied Biochemistry and Biotechnology - February 27, 2024 Category: Biochemistry Authors: Lv Zhu Shubo Li Jia-Yu Jiang Zhi-Yuan Yao Qing Li Shao-Jie Lian Qiang Liu Jin-Song Shi Zheng-Hong Xu Jin-Song Gong Source Type: research

Identification of the Beta Subunit Fas1p of Fatty Acid Synthetase as an Interacting Partner of Yeast Calcium/Calmodulin-Dependent Protein Kinase Cmk2p Through Mass Spectrometry Analysis
In this study, through these approaches, we have identified 65 and 110 potential Cmk2p-interacting proteins in yeast cells in the absence or presence of calcium stress, respectively. In yeast cells expressing both CMK2-HA and FAS1-GFP fusion proteins, in the absence or presence of calcium stress, less amounts of FAS1-GFP proteins are present in cell lysates after IP with anti-HA antibody than cell lysates before IP, while FAS1-GFP proteins are detected on both types of IP beads. However, as an internal control, similar amounts of Pgk1p proteins were detected in both after-IP and before-IP cell lysates but not on the IP bea...
Source: Applied Biochemistry and Biotechnology - February 27, 2024 Category: Biochemistry Authors: Linghuo Jiang Yiwu Li Yiying Gu Jiashi Zheng Liudan Wei Min Wei Jie Zou Chunyu Wei Bei Mo Lingxin Pan Lijiao Zhao Dan Wang Source Type: research

Investigation of Physicochemical Characteristics of Aspergillus niger Biomass and Examination of Its Ability to Separate Butyl Acetate Isomers
Appl Biochem Biotechnol. 2024 Feb 27. doi: 10.1007/s12010-024-04881-y. Online ahead of print.ABSTRACTAspergillus niger is a species of fungus that is widely found in natural ecosystems and has an important role in various industrial fields and is readily available. To study the adhesion of microbial cells to solid substrates and to improve their properties, physicochemical characterization of microorganisms is extremely important. For this purpose, in this study, the surface properties of A. niger biomass were determined at low cost and with high accuracy by inverse gas chromatography (IGC), a physicochemical characterizat...
Source: Applied Biochemistry and Biotechnology - February 27, 2024 Category: Biochemistry Authors: Birol Isik Mesut Bilgi Source Type: research

16S rDNA-Based Amplicon Analysis Unveiled a Correlation Between the Bacterial Diversity and Antibiotic Resistance Genes of Bacteriome of Commercial Smokeless Tobacco Products
Appl Biochem Biotechnol. 2024 Feb 26. doi: 10.1007/s12010-024-04857-y. Online ahead of print.ABSTRACTThe distribution of bacterial-derived antibiotic resistance genes (ARGs) in smokeless tobacco products is less explored and encourages understanding of the ARG profile of Indian smokeless tobacco products. Therefore, in the present investigation, ten commercial smokeless tobacco products were assessed for their bacterial diversity to understand the correlation between the inhabitant bacteria and predicted ARGs using a 16S rDNA-based metagenome analysis. Overall analysis showed the dominance of two phyla, i.e., Firmicutes (4...
Source: Applied Biochemistry and Biotechnology - February 26, 2024 Category: Biochemistry Authors: Akanksha Vishwakarma Digvijay Verma Source Type: research

High-Level Expression and Biochemical Characterization of a Maltotetraose Amylase in Pichia pastoris X-33 for Maltotetraose Production
In this study, high-level production of a maltotetraose amylase mutant (referred to as Pp-Mta∆CBM) from Pseudomonas saccharophila was achieved in Pichia pastoris X-33. First, the gene of maltotetraose amylase with the carbohydrate-binding module (CBM) removed was codon-optimized and cloned into the pPICZαA vector, followed by transformation into P. pastoris X-33 for expression. Using the promoter PAOX1 and signal peptide α-factor, high-level production of Pp-Mta∆CBM with minimal extracellular impurity proteins was achieved, resulting in an extracellular activity of 367.9 U/mL after 7 days of cultivation in shake flas...
Source: Applied Biochemistry and Biotechnology - February 26, 2024 Category: Biochemistry Authors: Xinyu Wang Ming Xu Xiaopeng Ren Mingyu Li Conggang Wang Fan Yang Xianzhen Li Source Type: research

Exonuclease-III Assisted the Target Recycling Coupling with Hybridization Chain Reaction for Sensitive mecA Gene Analysis by Using PGM
Appl Biochem Biotechnol. 2024 Feb 24. doi: 10.1007/s12010-024-04862-1. Online ahead of print.ABSTRACTIn the field of neonatal infections nursing, methicillin-resistant Staphylococcus aureus (MRSA) is a major bacterial pathogen. Here, we present a portable biosensor for MRSA detection that is both highly sensitive and portable, owing to its implementation on the personal glucose meter (PGM) platform. The H probe was fixed on the magnetic bead for mecA gene analysis. A blunt 3' terminus appeared in the MBs-H probe when the mecA gene was present. Exonuclease-III (Exo-III) recognized the blunt terminus and cleaved it, freeing ...
Source: Applied Biochemistry and Biotechnology - February 24, 2024 Category: Biochemistry Authors: Yan Du Ning Xiu Source Type: research