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A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Appl Biochem Biotechnol. 2023 Dec 16. doi: 10.1007/s12010-023-04806-1. Online ahead of print.ABSTRACTGene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological characteristics. Herein, we developed a rapid, efficient, and simple method for the knockout of long gene cassettes in Pseudomonas spp., based on a traditional allelic exchange strategy. The upstream and downstream sequences of the target gene cluster to be deleted were amplified using primers with 5'-end sequences identical to the multiple cloning sites of a suicide plasmid (...
Source: Applied Biochemistry and Biotechnology - December 16, 2023 Category: Biochemistry Authors: Feng Han Xiaoya Zhang Yunfei Chen Haixia Zhao Jieer Wu Yongxin Yu Yongjie Wang Source Type: research

A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Appl Biochem Biotechnol. 2023 Dec 16. doi: 10.1007/s12010-023-04806-1. Online ahead of print.ABSTRACTGene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological characteristics. Herein, we developed a rapid, efficient, and simple method for the knockout of long gene cassettes in Pseudomonas spp., based on a traditional allelic exchange strategy. The upstream and downstream sequences of the target gene cluster to be deleted were amplified using primers with 5'-end sequences identical to the multiple cloning sites of a suicide plasmid (...
Source: Applied Biochemistry and Biotechnology - December 16, 2023 Category: Biochemistry Authors: Feng Han Xiaoya Zhang Yunfei Chen Haixia Zhao Jieer Wu Yongxin Yu Yongjie Wang Source Type: research

A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Appl Biochem Biotechnol. 2023 Dec 16. doi: 10.1007/s12010-023-04806-1. Online ahead of print.ABSTRACTGene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological characteristics. Herein, we developed a rapid, efficient, and simple method for the knockout of long gene cassettes in Pseudomonas spp., based on a traditional allelic exchange strategy. The upstream and downstream sequences of the target gene cluster to be deleted were amplified using primers with 5'-end sequences identical to the multiple cloning sites of a suicide plasmid (...
Source: Applied Biochemistry and Biotechnology - December 16, 2023 Category: Biochemistry Authors: Feng Han Xiaoya Zhang Yunfei Chen Haixia Zhao Jieer Wu Yongxin Yu Yongjie Wang Source Type: research

A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Appl Biochem Biotechnol. 2023 Dec 16. doi: 10.1007/s12010-023-04806-1. Online ahead of print.ABSTRACTGene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological characteristics. Herein, we developed a rapid, efficient, and simple method for the knockout of long gene cassettes in Pseudomonas spp., based on a traditional allelic exchange strategy. The upstream and downstream sequences of the target gene cluster to be deleted were amplified using primers with 5'-end sequences identical to the multiple cloning sites of a suicide plasmid (...
Source: Applied Biochemistry and Biotechnology - December 16, 2023 Category: Biochemistry Authors: Feng Han Xiaoya Zhang Yunfei Chen Haixia Zhao Jieer Wu Yongxin Yu Yongjie Wang Source Type: research

circ-ATAD1 as Competing Endogenous RNA for miR-191-5p Forces Non-small Cell Lung Cancer Progression
Appl Biochem Biotechnol. 2023 Dec 11. doi: 10.1007/s12010-023-04770-w. Online ahead of print.ABSTRACTThe association of circular RNAs (circRNAs) with non-small cell lung cancer (NSCLC) has been recognized extensively. In view of this, our study particularly surveyed the underlying mechanism of circ-ATAD1 in the disease. First, an analysis of the clinical expression of circ-ATPase family AAA domain containing 1 (ATAD1) was performed, followed by further evaluation of the relationship between circ-ATAD1 expression and prognosis. Then, A549 cells were treated with single transfection or combined transfection with the plasmid ...
Source: Applied Biochemistry and Biotechnology - December 11, 2023 Category: Biochemistry Authors: Zhihua Wan Shanshan Jia Junhua Lu Xiangjing Ge Qinghua Chen Source Type: research