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Identification of Novel PI3K α Inhibitor Against Gastric Cancer: QSAR-, Molecular Docking-, and Molecular Dynamics Simulation-Based Analysis
Appl Biochem Biotechnol. 2024 Mar 20. doi: 10.1007/s12010-024-04898-3. Online ahead of print.ABSTRACTGastric cancer (GC) is a malignant tumor with global incidence and death ranking fifth and fourth, respectively. GC patients nevertheless have a poor prognosis despite the effectiveness of more advanced chemotherapy and surgical treatment options. The second most frequently mutated gene in GC is PI3Kalpha, a confirmed oncogene that results in abnormal PI3K/AKT/mTOR signaling, causing enhanced translation, proliferation, and survival, and is mutated in 7-25% of GC patients. The protein PI3Kalpha was targeted in the present s...
Source: Applied Biochemistry and Biotechnology - March 20, 2024 Category: Biochemistry Authors: Fang Yuan Ting Li Xinjie Xu Ting Chen Zhiqun Cao Source Type: research

LINC00963 Promotes Cisplatin Resistance in Esophageal Squamous Cell Carcinoma by Interacting with miR-10a to Upregulate SKA1 Expression
This study aims to investigate the effect of LINC00963 on cisplatin resistance in ESCC. After detecting the level of LINC00963 in human esophageal squamous epithelial cells (HET-1 A), ESCC cells (TE-1) and cisplatin resistant cells of ESCC (TE-1/DDP), TE-1/DDP cell line and nude mouse model that interfered with LINC00963 expression were established. Then, the interaction among LINC00963, miR-10a, and SKA1 was clarified by double luciferase and RNA immunoprecipitation (RIP) assays. Meanwhile, the biological behavior changes of TE-1/DDP cells with miR-10a overexpression or SKA1 silencing were observed by CCK-8, flow cytometr...
Source: Applied Biochemistry and Biotechnology - March 20, 2024 Category: Biochemistry Authors: Dongxin Hu Anqun Ma Hongda Lu Zhen Gao Yue Yu Jiaming Fan Shang Liu Yancheng Wang Mingyan Zhang Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research

SNORD45A Affects Content of HIF-1 α and Promotes Endothelial Angiogenic Function
Appl Biochem Biotechnol. 2024 Mar 15. doi: 10.1007/s12010-024-04916-4. Online ahead of print.ABSTRACTTo find out the differentially expressed small nucleolar RNAs (snoRNAs) in corneal neovascularization and their effect on angiogenesis. The rat model of corneal neovascularization induced by alkali burn was established, and the differentially expressed snoRNAs were sifted by high-throughput sequencing. Human genome homologs were screened and verified in cytopathological models. Polymerase chain reactions (PCRs) and Western blot assays were applied to detect mRNA and corresponding proteins affected by the differentially expr...
Source: Applied Biochemistry and Biotechnology - March 15, 2024 Category: Biochemistry Authors: Xi Yang Meng Li Hongqiao Wang Mengyuan Wang Yiming Liu Wenhua Xu Tao Jiang Source Type: research

Modulation of T Cell Differentiation in Mice with COPD Combined with Lung Cancer Through Key Targets of PD-1 by Tao Hong Si Wu Tang
The objective is to assess the anti-inflammatory effect of Tao Hong Si Wu Tang combined with anti-PD-1 in a mouse model of COPD combined with lung cancer, elucidating its mechanism through modulation of PD-1/PD-L binding, regulation of Th1/Th2 and Th17/Treg balance, inhibition of IL-4 and IL-17, and promotion of IFN-γ and TGF-β levels in peripheral blood. One hundred male C57/BL6 mice were randomly allocated to five groups: A (blank control), B (model control), C (THSW), D (anti-PD-1), and E (THSW + anti-PD-1), with 20 mice in each group. The COPD model was induced using fumigation and LPS intra-airway drip, followed by ...
Source: Applied Biochemistry and Biotechnology - March 15, 2024 Category: Biochemistry Authors: Guoli Wang Ge Wang Keming Zhao Aifeng Sui Lina Wang Yanling Xu Nini Qu Xiande Ma Hu Deng Source Type: research

The Potential of Fish Protein Hydrolysate Supplementation in Nile Tilapia Diets: Effects on Growth and Health Performance, Disease Resistance, and Farm Economic Analysis
Appl Biochem Biotechnol. 2024 Mar 15. doi: 10.1007/s12010-024-04913-7. Online ahead of print.ABSTRACTFish protein hydrolysate (FPH) has shown immense potential as a dietary protein supplement and immunostimulant in aquaculture, especially in Nile tilapia production. Four isoproteic diets (30% crude protein) were prepared by including FPH at varying percentages (0%, 0.5%, 1%, and 2%). Nile tilapia fed with FPH diets for 90 days, and their growth performance, feed utilization, blood biochemistry, liver and gut morphology, and resistance against Streptococcus iniae were investigated. The findings revealed that diets physical ...
Source: Applied Biochemistry and Biotechnology - March 15, 2024 Category: Biochemistry Authors: Muhammad Anamul Kabir Shishir Kumar Nandi Afrina Yeasmin Suma Zulhisyam Abdul Kari Suniza Anis Mohamad Sukri Lee Seong Wei Abdullah Al Mamun Paul Seguin Mikael Herault Martina Irwan Khoo Guillermo T éllez-Isaías Source Type: research

Effects of Hydrothermal Pretreatment and Anaerobic Digestion of Pig Manure on the Antibiotic Removal and Methane Production
This study investigated the outcome of antibiotics and methane productivity during pig manure treatment via HTP, AD, and HTP + AD. Results showed improved removal efficiency of sulfadiazine (SDZ), oxytetracycline (OTC), and enrofloxacin (ENR) with increased HTP temperatures (70, 90, 120, 150, and 170 °C). OTC achieved the highest removal efficiency of 86.8% at 170 °C because of its high sensitivity to heat treatment. For AD, SDZ exhibited resistance with a removal efficiency of 52.8%. However, OTC and ENR could be removed completely within 30 days. When HTP was used prior to AD, OTC and ENR could achieve complete removal...
Source: Applied Biochemistry and Biotechnology - March 14, 2024 Category: Biochemistry Authors: Chunchun Guo Yanfang Ma Yitao Li Zhiwu Wang Shupeng Lin Renjie Dong Shan Liu Source Type: research

Rutaecarpine Protects Against Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease (COPD) in Rats
Appl Biochem Biotechnol. 2024 Mar 14. doi: 10.1007/s12010-024-04896-5. Online ahead of print.ABSTRACTChronic obstructive pulmonary disease (COPD) is a chronic lung inflammatory disease that causes restricted airflow and breathing difficulties. In this work, we attempted to explore the salutary effects of rutaecarpine on COPD-induced rats. Healthy Wistar rats were employed in this study and exposed to cigarette smoke to initiate COPD. The rutaecarpine was given to the rats at 20 and 30 mg/kg dosages, respectively, for 12 weeks. Body weight gain, food uptake, and food efficiency were assessed after treatment completion. The ...
Source: Applied Biochemistry and Biotechnology - March 14, 2024 Category: Biochemistry Authors: Zexuan Ji Changhong Zhang Ping Feng Jianqing Zhao Source Type: research

Biofabricated Green Synthesized Hibiscus Silver Nanoparticles Potentiate Antibacterial Activity and Cytotoxicity in Human Lung Cancer Cells
Appl Biochem Biotechnol. 2024 Mar 14. doi: 10.1007/s12010-024-04901-x. Online ahead of print.ABSTRACTTraditional medicine has long employed the shrub Hibiscus sabdariffa to treat a variety of illnesses. The biochemical characteristics of silver nanoparticles made using the plant extract of Hibiscus sabdariffa were examined in this work. According to the results, the plant extract of Hibiscus sabdariffa had a total phenolic quantity of 84.9 mg/gm and a total quantity of flavonoids of 41.50 mg/gm. The extract also showed antibacterial action against Escherichia coli and Staphylococcus aureus (75.15% scavenging activity). The...
Source: Applied Biochemistry and Biotechnology - March 14, 2024 Category: Biochemistry Authors: Bharti Ahirwar Dheeraj Ahirwar Ritesh Jain Bhagyashri Agrawal Parvin Sahu Kalyani Sakure Hemant Badwaik Source Type: research

Effects of Hydrothermal Pretreatment and Anaerobic Digestion of Pig Manure on the Antibiotic Removal and Methane Production
This study investigated the outcome of antibiotics and methane productivity during pig manure treatment via HTP, AD, and HTP + AD. Results showed improved removal efficiency of sulfadiazine (SDZ), oxytetracycline (OTC), and enrofloxacin (ENR) with increased HTP temperatures (70, 90, 120, 150, and 170 °C). OTC achieved the highest removal efficiency of 86.8% at 170 °C because of its high sensitivity to heat treatment. For AD, SDZ exhibited resistance with a removal efficiency of 52.8%. However, OTC and ENR could be removed completely within 30 days. When HTP was used prior to AD, OTC and ENR could achieve complete removal...
Source: Applied Biochemistry and Biotechnology - March 14, 2024 Category: Biochemistry Authors: Chunchun Guo Yanfang Ma Yitao Li Zhiwu Wang Shupeng Lin Renjie Dong Shan Liu Source Type: research

Rutaecarpine Protects Against Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease (COPD) in Rats
Appl Biochem Biotechnol. 2024 Mar 14. doi: 10.1007/s12010-024-04896-5. Online ahead of print.ABSTRACTChronic obstructive pulmonary disease (COPD) is a chronic lung inflammatory disease that causes restricted airflow and breathing difficulties. In this work, we attempted to explore the salutary effects of rutaecarpine on COPD-induced rats. Healthy Wistar rats were employed in this study and exposed to cigarette smoke to initiate COPD. The rutaecarpine was given to the rats at 20 and 30 mg/kg dosages, respectively, for 12 weeks. Body weight gain, food uptake, and food efficiency were assessed after treatment completion. The ...
Source: Applied Biochemistry and Biotechnology - March 14, 2024 Category: Biochemistry Authors: Zexuan Ji Changhong Zhang Ping Feng Jianqing Zhao Source Type: research

Biofabricated Green Synthesized Hibiscus Silver Nanoparticles Potentiate Antibacterial Activity and Cytotoxicity in Human Lung Cancer Cells
Appl Biochem Biotechnol. 2024 Mar 14. doi: 10.1007/s12010-024-04901-x. Online ahead of print.ABSTRACTTraditional medicine has long employed the shrub Hibiscus sabdariffa to treat a variety of illnesses. The biochemical characteristics of silver nanoparticles made using the plant extract of Hibiscus sabdariffa were examined in this work. According to the results, the plant extract of Hibiscus sabdariffa had a total phenolic quantity of 84.9 mg/gm and a total quantity of flavonoids of 41.50 mg/gm. The extract also showed antibacterial action against Escherichia coli and Staphylococcus aureus (75.15% scavenging activity). The...
Source: Applied Biochemistry and Biotechnology - March 14, 2024 Category: Biochemistry Authors: Bharti Ahirwar Dheeraj Ahirwar Ritesh Jain Bhagyashri Agrawal Parvin Sahu Kalyani Sakure Hemant Badwaik Source Type: research