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Polyethylene Glycol-Based Polymer-Drug Conjugates: Novel Design and Synthesis Strategies for Enhanced Therapeutic Efficacy and Targeted Drug Delivery
This study seeks to investigate recent developments in the design and synthesis of PEG-based polymer-drug conjugates, emphasizing fresh ideas that fill in existing knowledge gaps and satisfy the increasing need for more potent drug delivery methods. Through an extensive review of the existing literature, this study identifies key challenges and proposes innovative strategies for future investigations. The paper presents a comprehensive framework for designing and synthesizing PEG-based polymer-drug conjugates, including rational molecular design, linker selection, conjugation methods, and characterization techniques. To fu...
Source: Applied Biochemistry and Biotechnology - March 23, 2024 Category: Biochemistry Authors: Vinay Sagar Verma Aakansha Pandey Arvind Kumar Jha Hemant Kumar Ramchandra Badwaik Amit Alexander None Ajazuddin Source Type: research

Metabolomics Approach to Explore Bioactive Natural Products Derived From Plant-Root-Associated Streptomyces
This study aims to screen for bioactive metabolites produced by Streptomyces strains in the plant rhizosphere.Six Streptomyces isolates were cultivated using three modified media to induce the production of diverse metabolites, employing the One Strain Many Compounds (OSMAC) approach. The metabolites present in extracts from fermentation broths were examined through a non-targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) approach coupled with Global Natural Products Social Molecular Networking (GNPS MN). The antimicrobial activity of the extracts was assessed using the disc diffusion method.The strains dem...
Source: Applied Biochemistry and Biotechnology - March 21, 2024 Category: Biochemistry Authors: Ekrem Kum Ebru İnce Source Type: research

Chemical Exposure of Synthetic Pyrethroid on Deltamethrin Under the Selection Pressure over the Generations: A Reproductive Potential Study of Anopheles stephensi
Appl Biochem Biotechnol. 2024 Mar 21. doi: 10.1007/s12010-024-04911-9. Online ahead of print.ABSTRACTBiochemical synthetic pyrethroids, deltamethrin are presently used insecticides for the control of mosquito vector-borne diseases in worldwide. Mosquito re-emergence with diseases becoming a serious problem due to development of insecticide resistance. The comprehensive knowledge on the underlying mechanisms of resistance against deltamethrin is required for implementation of an efficient vector control programme. The assessment of the biological fitness of a mosquito strain exposed to insecticide pressure is extremely vita...
Source: Applied Biochemistry and Biotechnology - March 21, 2024 Category: Biochemistry Authors: Palani Aarumugam Kalimuthu Kovendan Siva Kamalakannan Arulsamy Jebanesan Source Type: research

Biochemical Characterization of a Novel Thermostable 1,4- α-Glucoamylase from Aspergillus brasiliensis Strain Isolated in the Brazilian Atlantic Forest
Appl Biochem Biotechnol. 2024 Mar 21. doi: 10.1007/s12010-024-04903-9. Online ahead of print.ABSTRACTGlucoamylases are exo-enzymes that cleave the ends of the starch chain, releasing glucose units. In the current work, we described a novel 1,4-α-glucoamylase from an A. brasiliensis strain isolated from an environmental sample. The purified glucoamylase, GlaAb, has a molecular mass of 69 kDa and showed a starch binding domain. GlaAb showed a similar sequence to other fungal glucoamylases, and the molecular 3D model analysis of GlaAb suggests an overall structure as described in the literature, except by elongation in the l...
Source: Applied Biochemistry and Biotechnology - March 21, 2024 Category: Biochemistry Authors: Paula Zaghetto de Almeida Robson Carlos Alnoch Vanessa Elisa Pinheiro Marita Pereira Gimenez Maria de Lourdes Teixeira de Moraes Polizeli Source Type: research

Metabolomics Approach to Explore Bioactive Natural Products Derived From Plant-Root-Associated Streptomyces
This study aims to screen for bioactive metabolites produced by Streptomyces strains in the plant rhizosphere.Six Streptomyces isolates were cultivated using three modified media to induce the production of diverse metabolites, employing the One Strain Many Compounds (OSMAC) approach. The metabolites present in extracts from fermentation broths were examined through a non-targeted Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) approach coupled with Global Natural Products Social Molecular Networking (GNPS MN). The antimicrobial activity of the extracts was assessed using the disc diffusion method.The strains dem...
Source: Applied Biochemistry and Biotechnology - March 21, 2024 Category: Biochemistry Authors: Ekrem Kum Ebru İnce Source Type: research

Chemical Exposure of Synthetic Pyrethroid on Deltamethrin Under the Selection Pressure over the Generations: A Reproductive Potential Study of Anopheles stephensi
Appl Biochem Biotechnol. 2024 Mar 21. doi: 10.1007/s12010-024-04911-9. Online ahead of print.ABSTRACTBiochemical synthetic pyrethroids, deltamethrin are presently used insecticides for the control of mosquito vector-borne diseases in worldwide. Mosquito re-emergence with diseases becoming a serious problem due to development of insecticide resistance. The comprehensive knowledge on the underlying mechanisms of resistance against deltamethrin is required for implementation of an efficient vector control programme. The assessment of the biological fitness of a mosquito strain exposed to insecticide pressure is extremely vita...
Source: Applied Biochemistry and Biotechnology - March 21, 2024 Category: Biochemistry Authors: Palani Aarumugam Kalimuthu Kovendan Siva Kamalakannan Arulsamy Jebanesan Source Type: research

Biochemical Characterization of a Novel Thermostable 1,4- α-Glucoamylase from Aspergillus brasiliensis Strain Isolated in the Brazilian Atlantic Forest
Appl Biochem Biotechnol. 2024 Mar 21. doi: 10.1007/s12010-024-04903-9. Online ahead of print.ABSTRACTGlucoamylases are exo-enzymes that cleave the ends of the starch chain, releasing glucose units. In the current work, we described a novel 1,4-α-glucoamylase from an A. brasiliensis strain isolated from an environmental sample. The purified glucoamylase, GlaAb, has a molecular mass of 69 kDa and showed a starch binding domain. GlaAb showed a similar sequence to other fungal glucoamylases, and the molecular 3D model analysis of GlaAb suggests an overall structure as described in the literature, except by elongation in the l...
Source: Applied Biochemistry and Biotechnology - March 21, 2024 Category: Biochemistry Authors: Paula Zaghetto de Almeida Robson Carlos Alnoch Vanessa Elisa Pinheiro Marita Pereira Gimenez Maria de Lourdes Teixeira de Moraes Polizeli Source Type: research

Identification of Novel PI3K α Inhibitor Against Gastric Cancer: QSAR-, Molecular Docking-, and Molecular Dynamics Simulation-Based Analysis
Appl Biochem Biotechnol. 2024 Mar 20. doi: 10.1007/s12010-024-04898-3. Online ahead of print.ABSTRACTGastric cancer (GC) is a malignant tumor with global incidence and death ranking fifth and fourth, respectively. GC patients nevertheless have a poor prognosis despite the effectiveness of more advanced chemotherapy and surgical treatment options. The second most frequently mutated gene in GC is PI3Kalpha, a confirmed oncogene that results in abnormal PI3K/AKT/mTOR signaling, causing enhanced translation, proliferation, and survival, and is mutated in 7-25% of GC patients. The protein PI3Kalpha was targeted in the present s...
Source: Applied Biochemistry and Biotechnology - March 20, 2024 Category: Biochemistry Authors: Fang Yuan Ting Li Xinjie Xu Ting Chen Zhiqun Cao Source Type: research

LINC00963 Promotes Cisplatin Resistance in Esophageal Squamous Cell Carcinoma by Interacting with miR-10a to Upregulate SKA1 Expression
This study aims to investigate the effect of LINC00963 on cisplatin resistance in ESCC. After detecting the level of LINC00963 in human esophageal squamous epithelial cells (HET-1 A), ESCC cells (TE-1) and cisplatin resistant cells of ESCC (TE-1/DDP), TE-1/DDP cell line and nude mouse model that interfered with LINC00963 expression were established. Then, the interaction among LINC00963, miR-10a, and SKA1 was clarified by double luciferase and RNA immunoprecipitation (RIP) assays. Meanwhile, the biological behavior changes of TE-1/DDP cells with miR-10a overexpression or SKA1 silencing were observed by CCK-8, flow cytometr...
Source: Applied Biochemistry and Biotechnology - March 20, 2024 Category: Biochemistry Authors: Dongxin Hu Anqun Ma Hongda Lu Zhen Gao Yue Yu Jiaming Fan Shang Liu Yancheng Wang Mingyan Zhang Source Type: research

Identification of Novel PI3K α Inhibitor Against Gastric Cancer: QSAR-, Molecular Docking-, and Molecular Dynamics Simulation-Based Analysis
Appl Biochem Biotechnol. 2024 Mar 20. doi: 10.1007/s12010-024-04898-3. Online ahead of print.ABSTRACTGastric cancer (GC) is a malignant tumor with global incidence and death ranking fifth and fourth, respectively. GC patients nevertheless have a poor prognosis despite the effectiveness of more advanced chemotherapy and surgical treatment options. The second most frequently mutated gene in GC is PI3Kalpha, a confirmed oncogene that results in abnormal PI3K/AKT/mTOR signaling, causing enhanced translation, proliferation, and survival, and is mutated in 7-25% of GC patients. The protein PI3Kalpha was targeted in the present s...
Source: Applied Biochemistry and Biotechnology - March 20, 2024 Category: Biochemistry Authors: Fang Yuan Ting Li Xinjie Xu Ting Chen Zhiqun Cao Source Type: research

LINC00963 Promotes Cisplatin Resistance in Esophageal Squamous Cell Carcinoma by Interacting with miR-10a to Upregulate SKA1 Expression
This study aims to investigate the effect of LINC00963 on cisplatin resistance in ESCC. After detecting the level of LINC00963 in human esophageal squamous epithelial cells (HET-1 A), ESCC cells (TE-1) and cisplatin resistant cells of ESCC (TE-1/DDP), TE-1/DDP cell line and nude mouse model that interfered with LINC00963 expression were established. Then, the interaction among LINC00963, miR-10a, and SKA1 was clarified by double luciferase and RNA immunoprecipitation (RIP) assays. Meanwhile, the biological behavior changes of TE-1/DDP cells with miR-10a overexpression or SKA1 silencing were observed by CCK-8, flow cytometr...
Source: Applied Biochemistry and Biotechnology - March 20, 2024 Category: Biochemistry Authors: Dongxin Hu Anqun Ma Hongda Lu Zhen Gao Yue Yu Jiaming Fan Shang Liu Yancheng Wang Mingyan Zhang Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research

Unravelling Post-harvest Ripening Metabolomics of a New White Variety Guava Fruit (Cv Arka Mridula) with Special Emphasis on Phenolics and Corresponding Antioxidants
Appl Biochem Biotechnol. 2024 Mar 16. doi: 10.1007/s12010-024-04907-5. Online ahead of print.ABSTRACTThe phenolic, antioxidant and metabolic profiling of a new white variety guava fruit Arka Mridula (AM) was performed during its storage at the room temperature (28 ± 2 °C). The comparative profiles were generated at three ripening stages (pre-ripe, ripe and over-ripe) of the fruit. Generally, a steady decrease of the phenolic and antioxidant content from the pre-ripe to the ripe stage and a subsequent increase from the ripe to over-ripe stage was observed. Further, a powerful correlation between the phenolic content and a...
Source: Applied Biochemistry and Biotechnology - March 16, 2024 Category: Biochemistry Authors: Yatheesharadhya Bylappa Anish Nag Source Type: research